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Papers by Claudio Liberato

Research paper thumbnail of PCR-based assay for the mitochondrial cox1 specific amplification of Eucoleus böhmi

Veterinary Parasitology, 2015

Eucoleus böhmi (syn. Capillaria boehmi) is a trichuroid nematode affecting the epithelium of the ... more Eucoleus böhmi (syn. Capillaria boehmi) is a trichuroid nematode affecting the epithelium of the nasal turbinates, frontal and paranasal sinuses of wild and domestic canids. Knowledge of the geographic distribution of nasal eucoleosis is fragmentary, despite the infection has been described from Europe and North America. Moreover, gaps exist in information available on the importance of the disease in canine clinical practice. The lack of knowledge on E. böhmi is likely due to limitations inherent to diagnostic methodologies. The aim of the present work was to assess a PCR-based assay instrumental to the amplification of a species-specific region of the mitochondrial DNA (mtDNA) gene encoding for the subunit 1 (cox1) gene of E. böhmi. Adult worms of E. böhmi from red foxes and dogs from Norway, Serbia and Italy and individual fecal samples from naturally infected dogs from Italy were included in the study. Stool samples from dogs negative for E. böhmi, but positive for other common parasites in both single and mixed infections, and adult stages of common dog parasites, were used to assess the specificity of this genetic assay. Using the panel of faecal samples, the assay showed a sensitivity of 85.14% and a specificity of 100%.

Research paper thumbnail of Association between the 2001-2003 bluetongue epidemic in Lazio and Tuscany (central Italy) and distribution and abundance of Culicoides imicola and C. obsoletus vectors

Veterinaria italiana

During the epidemic of bluetongue (BT) in Lazio and Tuscany between 2001 and 2003, the distributi... more During the epidemic of bluetongue (BT) in Lazio and Tuscany between 2001 and 2003, the distribution pattern of Culicoides imicola did not always correspond either geographically or seasonally, with virus circulation. Culicoides obsoletus was observed to be abundant, ubiquitous and active throughout the year. The geographical and seasonal distribution of BT virus (BTV), C. imicola and C. obsoletus was compared. The territory of the two regions was divided into 30 cells each measuring 1 600 km(2). The presence of C. obsoletus was recorded in every cell, while C. imicola was detected in 18 of the 30 cells, but was absent in 6 of the 21 cells that indicated the presence of BTV. The occurrence of seroconversions appeared to be positively correlated with maximum C. obsoletus catches. Seroconversions were recorded throughout the year, even when C. imicola was not active, whereas C. obsoletus was detected during the entire period. The occurrence of BTV circulation in areas and periods where...

Research paper thumbnail of Identification of Culicoides obsoletus (Diptera: Ceratopogonidae) as a vector of bluetongue virus in central Italy

The Veterinary record, Jan 5, 2005

In 2001 and 2002, 235 outbreaks of bluetongue were observed in the Lazio and Tuscany regions of c... more In 2001 and 2002, 235 outbreaks of bluetongue were observed in the Lazio and Tuscany regions of central Italy. During entomological surveillance Culicoides imicola, the main vector of bluetongue virus in the Mediterranean region, was detected in only 14 of 28 municipalities affected by outbreaks; Culicoides obsoletus was the most abundant species, contributing 83 per cent of individuals in catches, whereas C. imicola contributed only 2 per cent. In affected municipalities the maximum catch of C. obsoletus was 18,000 specimens, compared with 54 of C. imicola. In October 2002 bluetongue virus serotype 2 was isolated from a single pool of wild-caught, non-blood-engorged parous C. obsoletus inoculated on to BHK-21 cells. Its identity was confirmed by reverse transcriptase-PCR.

Research paper thumbnail of Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing

Veterinary Parasitology, 2004

Research paper thumbnail of Investigation on Opisthorchis felineus occurrence and life cycle in Italy

Veterinary Parasitology, 2011

Research paper thumbnail of Genetic variation within and between G1 and G3 genotypes of Echinococcus granulosus in Italy revealed by multilocus DNA sequencing

Veterinary Parasitology, 2007

Research paper thumbnail of A study of the prevalence and genotypes of Giardia duodenalis infecting kennelled dogs

The Veterinary Journal, 2009

Research paper thumbnail of Geographical and seasonal distribution of the bluetongue virus vector, Culicoides imicola, in central Italy

Medical and Veterinary Entomology, 2003

Research paper thumbnail of Biotic and Abiotic Factors Influencing Distribution and Abundance of <I>Culicoides obsoletus</I> Group (Diptera: Ceratopogonidae) in Central Italy

Journal of Medical Entomology, 2010

Research paper thumbnail of Composition and diversity of helminth communities in eels Anguilla anguilla in the River Tiber: long-term changes and comparison with insular Europe

Journal of Helminthology, 1998

Research paper thumbnail of Presence of Anisakid Larvae in the European Anchovy, <I>Engraulis</I> <I>encrasicolus,</I> Fished Off the Tyrrhenian Coast of Central Italy

Journal of Food Protection, 2013

Research paper thumbnail of Incriminating bluetongue virus vectors with climate envelope models

Journal of Applied Ecology, 2007

Research paper thumbnail of Active circulation of bluetongue vaccine virus serotype-2 among unvaccinated cattle in central Italy

Preventive Veterinary Medicine, 2005

Several seroconversions occurring in 2002 among sentinel cattle during the bluetongue-vaccination... more Several seroconversions occurring in 2002 among sentinel cattle during the bluetongue-vaccination campaign in Lazio and Tuscany (central Italy) led to the suspicion of vaccine-virus circulation. Therefore in 2003, 17 seroconverting sentinel herds were investigated for the characteristics of the virus involved. From these farms, 91 unvaccinated animals and 57 Culicoides pools were tested for the presence of the bluetongue vaccine

Research paper thumbnail of Intestinal protozoans in Rupicapra spp. populations: baseline data in the framework of the relevant Italian project (PRIN)

Research paper thumbnail of PCR-based assay for the mitochondrial cox1 specific amplification of Eucoleus böhmi

Veterinary Parasitology, 2015

Eucoleus böhmi (syn. Capillaria boehmi) is a trichuroid nematode affecting the epithelium of the ... more Eucoleus böhmi (syn. Capillaria boehmi) is a trichuroid nematode affecting the epithelium of the nasal turbinates, frontal and paranasal sinuses of wild and domestic canids. Knowledge of the geographic distribution of nasal eucoleosis is fragmentary, despite the infection has been described from Europe and North America. Moreover, gaps exist in information available on the importance of the disease in canine clinical practice. The lack of knowledge on E. böhmi is likely due to limitations inherent to diagnostic methodologies. The aim of the present work was to assess a PCR-based assay instrumental to the amplification of a species-specific region of the mitochondrial DNA (mtDNA) gene encoding for the subunit 1 (cox1) gene of E. böhmi. Adult worms of E. böhmi from red foxes and dogs from Norway, Serbia and Italy and individual fecal samples from naturally infected dogs from Italy were included in the study. Stool samples from dogs negative for E. böhmi, but positive for other common parasites in both single and mixed infections, and adult stages of common dog parasites, were used to assess the specificity of this genetic assay. Using the panel of faecal samples, the assay showed a sensitivity of 85.14% and a specificity of 100%.

Research paper thumbnail of Association between the 2001-2003 bluetongue epidemic in Lazio and Tuscany (central Italy) and distribution and abundance of Culicoides imicola and C. obsoletus vectors

Veterinaria italiana

During the epidemic of bluetongue (BT) in Lazio and Tuscany between 2001 and 2003, the distributi... more During the epidemic of bluetongue (BT) in Lazio and Tuscany between 2001 and 2003, the distribution pattern of Culicoides imicola did not always correspond either geographically or seasonally, with virus circulation. Culicoides obsoletus was observed to be abundant, ubiquitous and active throughout the year. The geographical and seasonal distribution of BT virus (BTV), C. imicola and C. obsoletus was compared. The territory of the two regions was divided into 30 cells each measuring 1 600 km(2). The presence of C. obsoletus was recorded in every cell, while C. imicola was detected in 18 of the 30 cells, but was absent in 6 of the 21 cells that indicated the presence of BTV. The occurrence of seroconversions appeared to be positively correlated with maximum C. obsoletus catches. Seroconversions were recorded throughout the year, even when C. imicola was not active, whereas C. obsoletus was detected during the entire period. The occurrence of BTV circulation in areas and periods where...

Research paper thumbnail of Identification of Culicoides obsoletus (Diptera: Ceratopogonidae) as a vector of bluetongue virus in central Italy

The Veterinary record, Jan 5, 2005

In 2001 and 2002, 235 outbreaks of bluetongue were observed in the Lazio and Tuscany regions of c... more In 2001 and 2002, 235 outbreaks of bluetongue were observed in the Lazio and Tuscany regions of central Italy. During entomological surveillance Culicoides imicola, the main vector of bluetongue virus in the Mediterranean region, was detected in only 14 of 28 municipalities affected by outbreaks; Culicoides obsoletus was the most abundant species, contributing 83 per cent of individuals in catches, whereas C. imicola contributed only 2 per cent. In affected municipalities the maximum catch of C. obsoletus was 18,000 specimens, compared with 54 of C. imicola. In October 2002 bluetongue virus serotype 2 was isolated from a single pool of wild-caught, non-blood-engorged parous C. obsoletus inoculated on to BHK-21 cells. Its identity was confirmed by reverse transcriptase-PCR.

Research paper thumbnail of Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing

Veterinary Parasitology, 2004

Research paper thumbnail of Investigation on Opisthorchis felineus occurrence and life cycle in Italy

Veterinary Parasitology, 2011

Research paper thumbnail of Genetic variation within and between G1 and G3 genotypes of Echinococcus granulosus in Italy revealed by multilocus DNA sequencing

Veterinary Parasitology, 2007

Research paper thumbnail of A study of the prevalence and genotypes of Giardia duodenalis infecting kennelled dogs

The Veterinary Journal, 2009

Research paper thumbnail of Geographical and seasonal distribution of the bluetongue virus vector, Culicoides imicola, in central Italy

Medical and Veterinary Entomology, 2003

Research paper thumbnail of Biotic and Abiotic Factors Influencing Distribution and Abundance of <I>Culicoides obsoletus</I> Group (Diptera: Ceratopogonidae) in Central Italy

Journal of Medical Entomology, 2010

Research paper thumbnail of Composition and diversity of helminth communities in eels Anguilla anguilla in the River Tiber: long-term changes and comparison with insular Europe

Journal of Helminthology, 1998

Research paper thumbnail of Presence of Anisakid Larvae in the European Anchovy, <I>Engraulis</I> <I>encrasicolus,</I> Fished Off the Tyrrhenian Coast of Central Italy

Journal of Food Protection, 2013

Research paper thumbnail of Incriminating bluetongue virus vectors with climate envelope models

Journal of Applied Ecology, 2007

Research paper thumbnail of Active circulation of bluetongue vaccine virus serotype-2 among unvaccinated cattle in central Italy

Preventive Veterinary Medicine, 2005

Several seroconversions occurring in 2002 among sentinel cattle during the bluetongue-vaccination... more Several seroconversions occurring in 2002 among sentinel cattle during the bluetongue-vaccination campaign in Lazio and Tuscany (central Italy) led to the suspicion of vaccine-virus circulation. Therefore in 2003, 17 seroconverting sentinel herds were investigated for the characteristics of the virus involved. From these farms, 91 unvaccinated animals and 57 Culicoides pools were tested for the presence of the bluetongue vaccine

Research paper thumbnail of Intestinal protozoans in Rupicapra spp. populations: baseline data in the framework of the relevant Italian project (PRIN)