Colleen Scott - Academia.edu (original) (raw)

Papers by Colleen Scott

Research paper thumbnail of Childhood Bereavement: A Qualitative Study

The purpose of this dissertation is to examine the long-term effects of bereavement on adults who... more The purpose of this dissertation is to examine the long-term effects of bereavement on adults who experienced the death of a parent during childhood. By using a qualitative approach, this study is designed to gather information on each participant's unique understanding of how bereavement has impacted his or her life. This information is then used to identify general themes in

Research paper thumbnail of ChemInform Abstract: A Mild Synthesis of Unsymmetrical Bisalkoxysilanes Through Catalyzed Alcoholysis of Hydridosilanes Containing CC Multiple Bonds and Aryl Halides

Research paper thumbnail of A Mild Synthesis of Unsymmetrical Bis-Alkoxysilanes through Catalyzed Alcoholysis of Hydridosilanes

Synthesis-stuttgart, 2004

Research paper thumbnail of A Mild Synthesis of Unsymmetrical Bisalkoxysilanes through Catalyzed Alcoholysis of Hydridosilanes Containing C−C Multiple Bonds and Aryl Halides

Journal of Organic Chemistry, 2010

Research paper thumbnail of Synthesis and Optoelectronic Properties of a Reduced Band Gap Copolymer Derived from Silacyclopentadiene and Diketopyrrolopyrrole

Research paper thumbnail of Binding and Inhibition of Cdc25 Phosphatases by Vitamin K Analogues †

Biochemistry, 2003

A synthetic K vitamin analogue, 2-(2-mercaptothenol)-3-methyl-1,4-naphthoquinone or Cpd 5, was pr... more A synthetic K vitamin analogue, 2-(2-mercaptothenol)-3-methyl-1,4-naphthoquinone or Cpd 5, was previously found to be a potent inhibitor of cell growth [Nishikawa et al., (1995) J. Biol. Chem. 270, 28304-28310]. The mechanisms of cell growth were hypothesized to include the inactivation of cellular protein tyrosine phosphatases, especially the Cdc25 family [Tamura et al. (2000) Cancer Res. 60, 1317-1325]. In this study, we synthesized PD 49, a new biotin containing Cpd 5 derivative, to search for evidence of direct interaction of these arylating analogues with Cdc25A, Cdc25B, and Cdc25C phosphatases. PD 49 was shown to directly bind to GST-Cdc25A, GST-Cdc25B, their catalytic fragments, and GST-Cdc25C. The binding could be competed with excess glutathione or Cpd 5, and a cysteine-to-serine mutation of the catalytic cysteine abolished binding. This was consistent with an involvement in binding of cysteine in the catalytic domain. This interaction between PD 49 and Cdc25 also occurred in lysates of treated cells. PD 49 also bound to protein phosphatases other than Cdc25. We found that the new analogue also inhibited Hep3B human hepatoma cell growth. This growth inhibition involved ERK1/2 phosphorylation and was inhibited by a MEK antagonist. The results demonstrate a direct interaction and binding between this growth-inhibiting K vitamin derivative with both purified as well as with cellular Cdc25A, Cdc25B, and Cdc25C.

Research paper thumbnail of Childhood Bereavement: A Qualitative Study

The purpose of this dissertation is to examine the long-term effects of bereavement on adults who... more The purpose of this dissertation is to examine the long-term effects of bereavement on adults who experienced the death of a parent during childhood. By using a qualitative approach, this study is designed to gather information on each participant's unique understanding of how bereavement has impacted his or her life. This information is then used to identify general themes in

Research paper thumbnail of ChemInform Abstract: A Mild Synthesis of Unsymmetrical Bisalkoxysilanes Through Catalyzed Alcoholysis of Hydridosilanes Containing CC Multiple Bonds and Aryl Halides

Research paper thumbnail of A Mild Synthesis of Unsymmetrical Bis-Alkoxysilanes through Catalyzed Alcoholysis of Hydridosilanes

Synthesis-stuttgart, 2004

Research paper thumbnail of A Mild Synthesis of Unsymmetrical Bisalkoxysilanes through Catalyzed Alcoholysis of Hydridosilanes Containing C−C Multiple Bonds and Aryl Halides

Journal of Organic Chemistry, 2010

Research paper thumbnail of Synthesis and Optoelectronic Properties of a Reduced Band Gap Copolymer Derived from Silacyclopentadiene and Diketopyrrolopyrrole

Research paper thumbnail of Binding and Inhibition of Cdc25 Phosphatases by Vitamin K Analogues †

Biochemistry, 2003

A synthetic K vitamin analogue, 2-(2-mercaptothenol)-3-methyl-1,4-naphthoquinone or Cpd 5, was pr... more A synthetic K vitamin analogue, 2-(2-mercaptothenol)-3-methyl-1,4-naphthoquinone or Cpd 5, was previously found to be a potent inhibitor of cell growth [Nishikawa et al., (1995) J. Biol. Chem. 270, 28304-28310]. The mechanisms of cell growth were hypothesized to include the inactivation of cellular protein tyrosine phosphatases, especially the Cdc25 family [Tamura et al. (2000) Cancer Res. 60, 1317-1325]. In this study, we synthesized PD 49, a new biotin containing Cpd 5 derivative, to search for evidence of direct interaction of these arylating analogues with Cdc25A, Cdc25B, and Cdc25C phosphatases. PD 49 was shown to directly bind to GST-Cdc25A, GST-Cdc25B, their catalytic fragments, and GST-Cdc25C. The binding could be competed with excess glutathione or Cpd 5, and a cysteine-to-serine mutation of the catalytic cysteine abolished binding. This was consistent with an involvement in binding of cysteine in the catalytic domain. This interaction between PD 49 and Cdc25 also occurred in lysates of treated cells. PD 49 also bound to protein phosphatases other than Cdc25. We found that the new analogue also inhibited Hep3B human hepatoma cell growth. This growth inhibition involved ERK1/2 phosphorylation and was inhibited by a MEK antagonist. The results demonstrate a direct interaction and binding between this growth-inhibiting K vitamin derivative with both purified as well as with cellular Cdc25A, Cdc25B, and Cdc25C.