Cornelia Channing - Academia.edu (original) (raw)
Papers by Cornelia Channing
Journal of Steroid Biochemistry, 1979
... These observations along with the earlier studies of Ryan et al. [22, 23] support a twocell t... more ... These observations along with the earlier studies of Ryan et al. [22, 23] support a twocell theory of intrafollicu lar oestrogen biosynthesis. But the m vivo findings of Channing and Coudert[18] and the ultrastructural evidence of Crisp and Channing[25] and Meswerdt et u([24 ...
Human Reproduction, 1989
It has been reported that success at in-vitro fertilization/embryo transfer (IVF/ET) treatment is... more It has been reported that success at in-vitro fertilization/embryo transfer (IVF/ET) treatment is increased by follicular fluid (FF) re-injected into the abdomen. In the present study a possible direct effect of FF on human granulosa cell (GC) progesterone (P4) secretion and LH/human chorionic gonadotrophin (HCG) receptor content was studied in the presence and absence of FSH. Human GC cultured for 8 days in medium alone showed a 40-fold decrease in P4 secretion. Addition of human FSH increased P4 secretion and [125I]HCG specific binding by 12- and 8-fold, respectively, compared to human GC cultured in medium alone. The effect of FF was evaluated in a heterologous system by the addition of FF from large antral porcine follicles (LFF) to human GC in culture. The decline in human GC-P4 secretion after 8 days of culture was not altered by either porcine serum alone or porcine LFF alone. However, the concomitant addition of FSH and LFF significantly increased [125I]HCG specific binding, but did not alter the FSH-induced P4 secretion when both parameters were compared to GC cultured in FSH + porcine serum. Furthermore, the addition of HCG alone significantly increased P4 secretion 33- and 70-fold in GC pre-cultured with either FSH alone or FSH + LFF respectively compared with the stimulatory effect of HCG on GC pre-cultured in medium alone. These results may suggest that FSH and LFF increase the functional content of LH/HCG receptor in luteinized human GC.
Fertility and Sterility, 1984
Inhibin activity, follicle-stimulating hormone (FSH)-suppressing substance, estrogen, progesteron... more Inhibin activity, follicle-stimulating hormone (FSH)-suppressing substance, estrogen, progesterone, and androstenedione were measured in charcoal-treated ovarian tissue and ovarian venous and peripheral blood of eight rhesus monkeys ranging from 12 to 48 months of age. All of the monkeys demonstrated inhibin activity in ovarian tissue, which, if expressed per milligram protein, was relatively constant throughout development. However, if the activity was expressed per ovary, the amount of ovarian FSH-suppressing substance increased between 26 and 48 months; it was present in detectable amounts in ovarian venous blood only in one 26-month-old monkey. Detectable levels of estrogen were present in ovarian venous blood of the 26-month-old and the 48-month-old monkeys but not in the younger monkeys. It is possible that the secretion of inhibin activity may be in part responsible for low levels of serum FSH observed prior to puberty, because it has been observed by others that bilateral ovariectomy in the prepubertal monkey can result in a rise in FSH and that administration of charcoal-treated ovarian follicular fluid can suppress serum FSH in castrated prepubertal and adult rhesus monkeys.
Advances in Experimental Medicine and Biology, 1979
The injection of porcine follicular fluid (pFF1) can suppress both the rise in serum FSH that occ... more The injection of porcine follicular fluid (pFF1) can suppress both the rise in serum FSH that occurs in rats after ovariectomy (1) and FSH surges that occur in rats during proestrus (1600 h and 1900 h) and estrus (0800 h and 1100h)(2). Furthermore, it has been shown that pFF1 inhibits FSH secretion after LHRH infusion in phenobarbital-treated, proestrus rats (2), suggesting that pFFl exerts its suppressive effects at the level of the pituitary gland. Previously, it has been shown in vitro, using monolayers of pituitary cells, that non-steroidal substances of gonadal origin can directly inhibit gonadotropin responses to LHRH (3). Thus, the addition of rete testes fluid (free of steroids) to pituitary cells in a monolayer culture system resulted in an inhibition of both LH and FSH secretion in response to LHRH. The above findings prompted the present investigation which was designed to study the effects of charcoal-treated pFF1 on both basal and LHRH-stimulated secretion of FSH and LH from rat anterior pituitary cells cultured in monolayer.
Advances in Experimental Medicine and Biology, 1979
Porcine granulosa cells from different stages of follicular development were examined for their a... more Porcine granulosa cells from different stages of follicular development were examined for their ability to convert androgens to estrogens and their ability to secrete progesterone. Granulosa cells from all stages of follicular development can convert androgens to estrogens, and the addition of exogenous androgen is required for estrogen secretion. Granulosa cells obtained from medium and large follicles have a greater capacity to convert androgens to estrogens than do granulosa cells obtained from small follicles. The addition of FSH brought about an increase in estrogen secretion in the presence of androgen only in granulosa cells from large follicles. LH attenuated estrogen secretion in granulosa cells from medium follicles treated with testosterone. Granulosa cells from medium follicles were unable to secrete estrogen from days two to four irrespective of treatment. Androgens augment FSH stimulated progesterone secretion in granulosa cells from medium follicles from days two to four, and the addition of follicular fluid from small follicles stimulates progesterone secretion in the presence of FSH and androgens.
Clinics in Endocrinology and Metabolism, 1978
The ovulatory process can be regarded as a series of biochemical and morphological changes ultima... more The ovulatory process can be regarded as a series of biochemical and morphological changes ultimately leading to the release of a mature oocyte and the transformation of the Graafian follicle into the corpus luteum. This process involves acute changes in steroidogenesis, resumption of oocyte meiosis, and finally rupture of the follicular wall and luteinization of the granulosa cells. Normally, all of these changes are induced synchronously by the pre-ovulatory LH surge. Experimentally, however, these changes in steroidogenesis, oocyte maturation and follicular rupture can be dissociated from each other showing that the LH effect is mediated via different cellular messengers. The gonadotrophins act in an orderly sequence to induce follicular maturation. The granulosa cells increase their number of LH receptors and respond to LH with increased stimulation of cyclic AMP accumulation and progesterone secretion. Concomitantly, they decrease in their FSH receptors and their response to FSH diminishes in terms of ability to stimulate cyclic AMP accumulation. The ovulatory process is associated with increased uptake of LH by the follicle; when granulosa cells are obtained from pre-ovulatory follicles and cultured they luteinize spontaneously. Steroid hormones modulate the actions of gonadotrophins on follicular maturation. In addition, there are non-steroidal factors in follicular fluid which regulate follicular maturation: an oocyte maturation inhibitor keeps the oocyte in meiotic arrest; a luteinizing inhibitor prevents the granulosa cells from luteinizing prior to follicular rupture; a folliculostatin inhibits FSH release from the pituitary gland. The functional activity and the lifespan of the corpus luteum depend on adequate pre-ovulatory as well as post-ovulatory gonadotrophic stimulation. Its lifespan may also be regulated by an LH binding inhibitor.
Proceedings of the 1969 Laurentian Hormone Conference, 1970
Publisher Summary In this chapter, in vitro culture of granulosa cells has been chosen as the mod... more Publisher Summary In this chapter, in vitro culture of granulosa cells has been chosen as the model system to study the mechanism of luteinization because spontaneous luteinization has been observed in tissue cultures of equine, porcine human, and rhesus monkey granulosa cells. In a study described in the chapter, in each species, luteinization was observed only in cultures of granulosa cells harvested from large preovulatory follicles, not in cultures of cells harvested from smaller follicles. Granulosa cells were said to luteinize in culture if they accumulated eosinophilic granules and lipid droplets in the cytoplasm, increased in size and cytoplasmic-nuclear ratio, and secreted large amounts of progesterone. The chapter presents results of studies primarily on the rhesus monkey in addition to several other species and also presents a unified theory of the mechanism of luteinization. Granulosa cells from the monkey, pig, human, and mare most likely luteinize according to similar mechanisms. Pituitary gonadotropins appear to be required for the process. Cyclic 3', 5'-AMP is probably a mediator for morphologic and steroidogenic luteinization.
Advances in Experimental Medicine and Biology, 1979
Chemical messengers synthesized both within and without the ovary control follicular and oocyte m... more Chemical messengers synthesized both within and without the ovary control follicular and oocyte maturation and ovulation. The extraovarian messengers, whose roles are better defined, are the pituitary gonadotropins-luteinizing hormone (LH) and follicle stimulating hormone (FSH). Other hormones-insulin, cortisol, thyroxin, and prolactin have poorly defined “permissive” functions. Intraovarian chemical messengers include ovarian steroids (estrogen, progesterone, and androgens) and polypeptide regulators such as oocyte maturation inhibitor (OMI), luteinization inhibitor (LI) ovarian inhibin (folliculostatin), luteinizing hormone binding inhibitor (LHRBI), and a possible follicular fluid luteinizing promoting substance.
Gamete Research, 1980
... Torbjorn Hillensjo and Cornelia P. Channing ... cumuli in culture brings about cellular dispe... more ... Torbjorn Hillensjo and Cornelia P. Channing ... cumuli in culture brings about cellular dispersion in several species including rabbit [Thibault, 19721 , mouse [Neal and Baker, 1973; Eppig, 1979a], rat [ Hillensjo et al, 1976; Dekel and Kraicer, 19781 and sheep [Moor and Trounson ...
Proceedings of the 1981 Laurentian Hormone Conference, 1982
1: Recent Prog Horm Res. 1982;38:331-408. The role of nonsteroidal regulators in control of oocyt... more 1: Recent Prog Horm Res. 1982;38:331-408. The role of nonsteroidal regulators in control of oocyte and follicular maturation. Channing CP, Anderson LD, Hoover DJ, Kolena J, Osteen KG, Pomerantz SH, Tanabe K. ...
Gamete Research, 1985
Page 1. Gamete Research 12: 119-130 (1985) Influence of Hormones on the Inhibitory Activity of Oo... more Page 1. Gamete Research 12: 119-130 (1985) Influence of Hormones on the Inhibitory Activity of Oocyte Maturation Present in Conditioned Media of Porcine Granulosa Cells Larry D. Anderson, Sarah Lipford Stone, and Cornelia P. Channing ...
Advances in Experimental Medicine and Biology, 1979
As porcine corpus luteum tissue ages there is an increase in amount of LH/hCG vinding inhibitor. ... more As porcine corpus luteum tissue ages there is an increase in amount of LH/hCG vinding inhibitor. This inhibitor is absent in non-luteal ovarian tissue, heart and lung.
Proceedings of the National Academy of Sciences, 1983
To examine whether a decline in follicular oocyte maturation inhibitor (OMI) is associated with a... more To examine whether a decline in follicular oocyte maturation inhibitor (OMI) is associated with attainment of oocyte maturation and fertilizability, OMI was measured in follicular fluid (FF) of 39 follicles of 20 normal women given human menopausal gonadotrophin and human chorionic gonadotrophin to induce follicular growth and maturation. Oocytes were aspirated per laparoscope, the fluid was saved, and the egg was observed, incubated, and inseminated with the husband's sperm. Concepti that developed to the 4- to 8-cell stage were transferred to the uterus and the women were followed for pregnancy. OMI activity in each FF was measured by using cultured cumulus-enclosed porcine oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, and delta 4-androstenedione were measured in FF by radioimmunoassay. The FF of 13 preovulatory follicles yielding oocytes that were mature and fertilizable had significantly less OMI...
Proceedings of the National Academy of Sciences, 1977
The present studies were carried out to see if porcine follicular fluid could inhibit increases i... more The present studies were carried out to see if porcine follicular fluid could inhibit increases in serum follicle stimulating hormone (FSH) levels when injected into the rat. For these studies the pentobarbital-treated proestrous rat was chosen as the major test animal model. If an artificial surge of luteinizing hormone (LH) is administered to these rats, it can induce a synchronized secondary rise in FSH secretion rate. Normal saline-treated rats were also used as test animals. They exhibit preovulatory endogenous “surges” of LH and FSH, and also a secondary FSH rise. Porcine follicular fluid was harvested from medium-sized and large (3- to 10-mm diameter) follicles and treated with charcoal to remove endogenous steroids. Charcoal-treated porcine serum served as a control solution. The fluid was injected intraperitoneally in two 0.5-ml doses into pentobarbital-treated proestrous rats immediately and 3 hr after LH injection. Follicular fluid, but not the serum, suppressed the secon...
Metabolism, 1977
The mechanism of action of luteinizing folliculogenesis and follicular growth, hormone (LH) and f... more The mechanism of action of luteinizing folliculogenesis and follicular growth, hormone (LH) and follicle-stimulating hor-oocyte maturation, follicular rupture, and mone (FSH) upon various cell types of the corpus luteum maintenance and steroidomammalian ovary is reviewed. Emphasis genesis. The roles of gonadotropin reis placed upon in vitro studies using organ ceptors, AMP, prostaglandins, protein and cell culture as well as short-term in-kinase, and protein synthesis in these LH cubations. FSH and LH actions upon the and FSH actions are discussed. Intrafollowing ovarian functions are discussed: ovarian regulation of LH and FSH action steroidogenesis and metabolism of the is reviewed, including a discussion of the ovary as a whole and of the isolated possible roles of follicular fluid inhibitors follicle and its component cell types, the upon oocyte maturation and granulosa cell granulosa and thecal cells, as well as luteinization.
Journal of Endocrinology, 1979
The effects of ovine, porcine and human FSH, and ovine and human LH on the accumulation of cyclic... more The effects of ovine, porcine and human FSH, and ovine and human LH on the accumulation of cyclic AMP by porcine granulosa cells obtained from follicles at various stages of maturation were investigated. During incubation periods of 15 min, 10 μg ovine FSH pretreated with antiserum to LH or 10 μg human FSH resulted in an 11- to 18-fold, five-to ninefold, and less than a twofold increase in intracellular accumulation of cyclic AMP by granulosa cells from small (1–2 mm), medium (3–5 mm) and large (6–12 mm) follicles respectively. Similar patterns of response occurred with addition of porcine FSH. After incubation for 30 and 60 min with ovine, porcine or human FSH, significant accumulation of cyclic AMP in the incubation medium occurred with cells obtained from small and medium-sized follicles. After 60 min of incubation with FSH the accumulation of cyclic AMP in the incubation medium exceeded the intracellular cyclic AMP levels in granulosa cells from small and medium-sized follicles....
The Journal of Clinical Endocrinology & Metabolism, 1978
Human follicular fluid (hFFl) was harvested from follicles (5-15 mm) of human ovaries obtained at... more Human follicular fluid (hFFl) was harvested from follicles (5-15 mm) of human ovaries obtained at laparotomy. Addition of native hFFl, or a low molecular weight fraction of it, to the culture medium was found to inhibit the spontaneous maturation of cumulus-enclosed porcine oocytes. It was also found that hFFl inhibited progesterone secretion by the cumulus cells. The results extend earlier observations in other mammalian species, indicating that in the human, also, a specific oocyte maturation inhibitor is responsible for keeping the oocyte in meiotic arrest.
The Journal of Clinical Endocrinology & Metabolism, 1981
Abstract To examine what relationships exist between human follicular fluid inhibin activity and ... more Abstract To examine what relationships exist between human follicular fluid inhibin activity and steroid content, follicular fluid was aspirated from 72 follicles at various stages of follicular development. Twenty samples were collected from cystic ovarian follicles, and 50 ...
Hormone Research, 1985
Follicular fluid obtained from large (6-12 mm) porcine follicles (LFF) was investigated to determ... more Follicular fluid obtained from large (6-12 mm) porcine follicles (LFF) was investigated to determine its stimulatory activity on progesterone secretion and on follicle stimulating hormone (FSH) induction of 125I-human chorionic gonadotropin (hCG)-luteinizing hormone (LH) binding sites in porcine granulosa cells in a 4-day culture. Incubation of granulosa cells harvested from small porcine follicles (1-2 mm) with 50% LFF led to stimulation of LH/hCG binding sites and progesterone secretion. After partial purification of pooled LFF or proteins precipitated with 90% ethanol on Sephadex G-100 the greatest stimulatory activity was found in the second protein peak eluted from the column. Chromatography of part of the active fraction on DEAE Sephacel using a continuous gradient of NH4HCO3 yielded seven protein fractions. The second fraction, which eluted early, contained the majority of the stimulatory activity which was purified about 32-fold compared to native LFF. In contrast, addition of follicular fluid recovered from small porcine follicles inhibited FSH induction of LH/hCG binding sites and progesterone secretion. It can be concluded, that maturation of granulosa cells from small follicles may be enhanced by protein(s) present in LFF, but not in fluid recovered from less mature follicles.
Journal of Steroid Biochemistry, 1979
... These observations along with the earlier studies of Ryan et al. [22, 23] support a twocell t... more ... These observations along with the earlier studies of Ryan et al. [22, 23] support a twocell theory of intrafollicu lar oestrogen biosynthesis. But the m vivo findings of Channing and Coudert[18] and the ultrastructural evidence of Crisp and Channing[25] and Meswerdt et u([24 ...
Human Reproduction, 1989
It has been reported that success at in-vitro fertilization/embryo transfer (IVF/ET) treatment is... more It has been reported that success at in-vitro fertilization/embryo transfer (IVF/ET) treatment is increased by follicular fluid (FF) re-injected into the abdomen. In the present study a possible direct effect of FF on human granulosa cell (GC) progesterone (P4) secretion and LH/human chorionic gonadotrophin (HCG) receptor content was studied in the presence and absence of FSH. Human GC cultured for 8 days in medium alone showed a 40-fold decrease in P4 secretion. Addition of human FSH increased P4 secretion and [125I]HCG specific binding by 12- and 8-fold, respectively, compared to human GC cultured in medium alone. The effect of FF was evaluated in a heterologous system by the addition of FF from large antral porcine follicles (LFF) to human GC in culture. The decline in human GC-P4 secretion after 8 days of culture was not altered by either porcine serum alone or porcine LFF alone. However, the concomitant addition of FSH and LFF significantly increased [125I]HCG specific binding, but did not alter the FSH-induced P4 secretion when both parameters were compared to GC cultured in FSH + porcine serum. Furthermore, the addition of HCG alone significantly increased P4 secretion 33- and 70-fold in GC pre-cultured with either FSH alone or FSH + LFF respectively compared with the stimulatory effect of HCG on GC pre-cultured in medium alone. These results may suggest that FSH and LFF increase the functional content of LH/HCG receptor in luteinized human GC.
Fertility and Sterility, 1984
Inhibin activity, follicle-stimulating hormone (FSH)-suppressing substance, estrogen, progesteron... more Inhibin activity, follicle-stimulating hormone (FSH)-suppressing substance, estrogen, progesterone, and androstenedione were measured in charcoal-treated ovarian tissue and ovarian venous and peripheral blood of eight rhesus monkeys ranging from 12 to 48 months of age. All of the monkeys demonstrated inhibin activity in ovarian tissue, which, if expressed per milligram protein, was relatively constant throughout development. However, if the activity was expressed per ovary, the amount of ovarian FSH-suppressing substance increased between 26 and 48 months; it was present in detectable amounts in ovarian venous blood only in one 26-month-old monkey. Detectable levels of estrogen were present in ovarian venous blood of the 26-month-old and the 48-month-old monkeys but not in the younger monkeys. It is possible that the secretion of inhibin activity may be in part responsible for low levels of serum FSH observed prior to puberty, because it has been observed by others that bilateral ovariectomy in the prepubertal monkey can result in a rise in FSH and that administration of charcoal-treated ovarian follicular fluid can suppress serum FSH in castrated prepubertal and adult rhesus monkeys.
Advances in Experimental Medicine and Biology, 1979
The injection of porcine follicular fluid (pFF1) can suppress both the rise in serum FSH that occ... more The injection of porcine follicular fluid (pFF1) can suppress both the rise in serum FSH that occurs in rats after ovariectomy (1) and FSH surges that occur in rats during proestrus (1600 h and 1900 h) and estrus (0800 h and 1100h)(2). Furthermore, it has been shown that pFF1 inhibits FSH secretion after LHRH infusion in phenobarbital-treated, proestrus rats (2), suggesting that pFFl exerts its suppressive effects at the level of the pituitary gland. Previously, it has been shown in vitro, using monolayers of pituitary cells, that non-steroidal substances of gonadal origin can directly inhibit gonadotropin responses to LHRH (3). Thus, the addition of rete testes fluid (free of steroids) to pituitary cells in a monolayer culture system resulted in an inhibition of both LH and FSH secretion in response to LHRH. The above findings prompted the present investigation which was designed to study the effects of charcoal-treated pFF1 on both basal and LHRH-stimulated secretion of FSH and LH from rat anterior pituitary cells cultured in monolayer.
Advances in Experimental Medicine and Biology, 1979
Porcine granulosa cells from different stages of follicular development were examined for their a... more Porcine granulosa cells from different stages of follicular development were examined for their ability to convert androgens to estrogens and their ability to secrete progesterone. Granulosa cells from all stages of follicular development can convert androgens to estrogens, and the addition of exogenous androgen is required for estrogen secretion. Granulosa cells obtained from medium and large follicles have a greater capacity to convert androgens to estrogens than do granulosa cells obtained from small follicles. The addition of FSH brought about an increase in estrogen secretion in the presence of androgen only in granulosa cells from large follicles. LH attenuated estrogen secretion in granulosa cells from medium follicles treated with testosterone. Granulosa cells from medium follicles were unable to secrete estrogen from days two to four irrespective of treatment. Androgens augment FSH stimulated progesterone secretion in granulosa cells from medium follicles from days two to four, and the addition of follicular fluid from small follicles stimulates progesterone secretion in the presence of FSH and androgens.
Clinics in Endocrinology and Metabolism, 1978
The ovulatory process can be regarded as a series of biochemical and morphological changes ultima... more The ovulatory process can be regarded as a series of biochemical and morphological changes ultimately leading to the release of a mature oocyte and the transformation of the Graafian follicle into the corpus luteum. This process involves acute changes in steroidogenesis, resumption of oocyte meiosis, and finally rupture of the follicular wall and luteinization of the granulosa cells. Normally, all of these changes are induced synchronously by the pre-ovulatory LH surge. Experimentally, however, these changes in steroidogenesis, oocyte maturation and follicular rupture can be dissociated from each other showing that the LH effect is mediated via different cellular messengers. The gonadotrophins act in an orderly sequence to induce follicular maturation. The granulosa cells increase their number of LH receptors and respond to LH with increased stimulation of cyclic AMP accumulation and progesterone secretion. Concomitantly, they decrease in their FSH receptors and their response to FSH diminishes in terms of ability to stimulate cyclic AMP accumulation. The ovulatory process is associated with increased uptake of LH by the follicle; when granulosa cells are obtained from pre-ovulatory follicles and cultured they luteinize spontaneously. Steroid hormones modulate the actions of gonadotrophins on follicular maturation. In addition, there are non-steroidal factors in follicular fluid which regulate follicular maturation: an oocyte maturation inhibitor keeps the oocyte in meiotic arrest; a luteinizing inhibitor prevents the granulosa cells from luteinizing prior to follicular rupture; a folliculostatin inhibits FSH release from the pituitary gland. The functional activity and the lifespan of the corpus luteum depend on adequate pre-ovulatory as well as post-ovulatory gonadotrophic stimulation. Its lifespan may also be regulated by an LH binding inhibitor.
Proceedings of the 1969 Laurentian Hormone Conference, 1970
Publisher Summary In this chapter, in vitro culture of granulosa cells has been chosen as the mod... more Publisher Summary In this chapter, in vitro culture of granulosa cells has been chosen as the model system to study the mechanism of luteinization because spontaneous luteinization has been observed in tissue cultures of equine, porcine human, and rhesus monkey granulosa cells. In a study described in the chapter, in each species, luteinization was observed only in cultures of granulosa cells harvested from large preovulatory follicles, not in cultures of cells harvested from smaller follicles. Granulosa cells were said to luteinize in culture if they accumulated eosinophilic granules and lipid droplets in the cytoplasm, increased in size and cytoplasmic-nuclear ratio, and secreted large amounts of progesterone. The chapter presents results of studies primarily on the rhesus monkey in addition to several other species and also presents a unified theory of the mechanism of luteinization. Granulosa cells from the monkey, pig, human, and mare most likely luteinize according to similar mechanisms. Pituitary gonadotropins appear to be required for the process. Cyclic 3', 5'-AMP is probably a mediator for morphologic and steroidogenic luteinization.
Advances in Experimental Medicine and Biology, 1979
Chemical messengers synthesized both within and without the ovary control follicular and oocyte m... more Chemical messengers synthesized both within and without the ovary control follicular and oocyte maturation and ovulation. The extraovarian messengers, whose roles are better defined, are the pituitary gonadotropins-luteinizing hormone (LH) and follicle stimulating hormone (FSH). Other hormones-insulin, cortisol, thyroxin, and prolactin have poorly defined “permissive” functions. Intraovarian chemical messengers include ovarian steroids (estrogen, progesterone, and androgens) and polypeptide regulators such as oocyte maturation inhibitor (OMI), luteinization inhibitor (LI) ovarian inhibin (folliculostatin), luteinizing hormone binding inhibitor (LHRBI), and a possible follicular fluid luteinizing promoting substance.
Gamete Research, 1980
... Torbjorn Hillensjo and Cornelia P. Channing ... cumuli in culture brings about cellular dispe... more ... Torbjorn Hillensjo and Cornelia P. Channing ... cumuli in culture brings about cellular dispersion in several species including rabbit [Thibault, 19721 , mouse [Neal and Baker, 1973; Eppig, 1979a], rat [ Hillensjo et al, 1976; Dekel and Kraicer, 19781 and sheep [Moor and Trounson ...
Proceedings of the 1981 Laurentian Hormone Conference, 1982
1: Recent Prog Horm Res. 1982;38:331-408. The role of nonsteroidal regulators in control of oocyt... more 1: Recent Prog Horm Res. 1982;38:331-408. The role of nonsteroidal regulators in control of oocyte and follicular maturation. Channing CP, Anderson LD, Hoover DJ, Kolena J, Osteen KG, Pomerantz SH, Tanabe K. ...
Gamete Research, 1985
Page 1. Gamete Research 12: 119-130 (1985) Influence of Hormones on the Inhibitory Activity of Oo... more Page 1. Gamete Research 12: 119-130 (1985) Influence of Hormones on the Inhibitory Activity of Oocyte Maturation Present in Conditioned Media of Porcine Granulosa Cells Larry D. Anderson, Sarah Lipford Stone, and Cornelia P. Channing ...
Advances in Experimental Medicine and Biology, 1979
As porcine corpus luteum tissue ages there is an increase in amount of LH/hCG vinding inhibitor. ... more As porcine corpus luteum tissue ages there is an increase in amount of LH/hCG vinding inhibitor. This inhibitor is absent in non-luteal ovarian tissue, heart and lung.
Proceedings of the National Academy of Sciences, 1983
To examine whether a decline in follicular oocyte maturation inhibitor (OMI) is associated with a... more To examine whether a decline in follicular oocyte maturation inhibitor (OMI) is associated with attainment of oocyte maturation and fertilizability, OMI was measured in follicular fluid (FF) of 39 follicles of 20 normal women given human menopausal gonadotrophin and human chorionic gonadotrophin to induce follicular growth and maturation. Oocytes were aspirated per laparoscope, the fluid was saved, and the egg was observed, incubated, and inseminated with the husband's sperm. Concepti that developed to the 4- to 8-cell stage were transferred to the uterus and the women were followed for pregnancy. OMI activity in each FF was measured by using cultured cumulus-enclosed porcine oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, and delta 4-androstenedione were measured in FF by radioimmunoassay. The FF of 13 preovulatory follicles yielding oocytes that were mature and fertilizable had significantly less OMI...
Proceedings of the National Academy of Sciences, 1977
The present studies were carried out to see if porcine follicular fluid could inhibit increases i... more The present studies were carried out to see if porcine follicular fluid could inhibit increases in serum follicle stimulating hormone (FSH) levels when injected into the rat. For these studies the pentobarbital-treated proestrous rat was chosen as the major test animal model. If an artificial surge of luteinizing hormone (LH) is administered to these rats, it can induce a synchronized secondary rise in FSH secretion rate. Normal saline-treated rats were also used as test animals. They exhibit preovulatory endogenous “surges” of LH and FSH, and also a secondary FSH rise. Porcine follicular fluid was harvested from medium-sized and large (3- to 10-mm diameter) follicles and treated with charcoal to remove endogenous steroids. Charcoal-treated porcine serum served as a control solution. The fluid was injected intraperitoneally in two 0.5-ml doses into pentobarbital-treated proestrous rats immediately and 3 hr after LH injection. Follicular fluid, but not the serum, suppressed the secon...
Metabolism, 1977
The mechanism of action of luteinizing folliculogenesis and follicular growth, hormone (LH) and f... more The mechanism of action of luteinizing folliculogenesis and follicular growth, hormone (LH) and follicle-stimulating hor-oocyte maturation, follicular rupture, and mone (FSH) upon various cell types of the corpus luteum maintenance and steroidomammalian ovary is reviewed. Emphasis genesis. The roles of gonadotropin reis placed upon in vitro studies using organ ceptors, AMP, prostaglandins, protein and cell culture as well as short-term in-kinase, and protein synthesis in these LH cubations. FSH and LH actions upon the and FSH actions are discussed. Intrafollowing ovarian functions are discussed: ovarian regulation of LH and FSH action steroidogenesis and metabolism of the is reviewed, including a discussion of the ovary as a whole and of the isolated possible roles of follicular fluid inhibitors follicle and its component cell types, the upon oocyte maturation and granulosa cell granulosa and thecal cells, as well as luteinization.
Journal of Endocrinology, 1979
The effects of ovine, porcine and human FSH, and ovine and human LH on the accumulation of cyclic... more The effects of ovine, porcine and human FSH, and ovine and human LH on the accumulation of cyclic AMP by porcine granulosa cells obtained from follicles at various stages of maturation were investigated. During incubation periods of 15 min, 10 μg ovine FSH pretreated with antiserum to LH or 10 μg human FSH resulted in an 11- to 18-fold, five-to ninefold, and less than a twofold increase in intracellular accumulation of cyclic AMP by granulosa cells from small (1–2 mm), medium (3–5 mm) and large (6–12 mm) follicles respectively. Similar patterns of response occurred with addition of porcine FSH. After incubation for 30 and 60 min with ovine, porcine or human FSH, significant accumulation of cyclic AMP in the incubation medium occurred with cells obtained from small and medium-sized follicles. After 60 min of incubation with FSH the accumulation of cyclic AMP in the incubation medium exceeded the intracellular cyclic AMP levels in granulosa cells from small and medium-sized follicles....
The Journal of Clinical Endocrinology & Metabolism, 1978
Human follicular fluid (hFFl) was harvested from follicles (5-15 mm) of human ovaries obtained at... more Human follicular fluid (hFFl) was harvested from follicles (5-15 mm) of human ovaries obtained at laparotomy. Addition of native hFFl, or a low molecular weight fraction of it, to the culture medium was found to inhibit the spontaneous maturation of cumulus-enclosed porcine oocytes. It was also found that hFFl inhibited progesterone secretion by the cumulus cells. The results extend earlier observations in other mammalian species, indicating that in the human, also, a specific oocyte maturation inhibitor is responsible for keeping the oocyte in meiotic arrest.
The Journal of Clinical Endocrinology & Metabolism, 1981
Abstract To examine what relationships exist between human follicular fluid inhibin activity and ... more Abstract To examine what relationships exist between human follicular fluid inhibin activity and steroid content, follicular fluid was aspirated from 72 follicles at various stages of follicular development. Twenty samples were collected from cystic ovarian follicles, and 50 ...
Hormone Research, 1985
Follicular fluid obtained from large (6-12 mm) porcine follicles (LFF) was investigated to determ... more Follicular fluid obtained from large (6-12 mm) porcine follicles (LFF) was investigated to determine its stimulatory activity on progesterone secretion and on follicle stimulating hormone (FSH) induction of 125I-human chorionic gonadotropin (hCG)-luteinizing hormone (LH) binding sites in porcine granulosa cells in a 4-day culture. Incubation of granulosa cells harvested from small porcine follicles (1-2 mm) with 50% LFF led to stimulation of LH/hCG binding sites and progesterone secretion. After partial purification of pooled LFF or proteins precipitated with 90% ethanol on Sephadex G-100 the greatest stimulatory activity was found in the second protein peak eluted from the column. Chromatography of part of the active fraction on DEAE Sephacel using a continuous gradient of NH4HCO3 yielded seven protein fractions. The second fraction, which eluted early, contained the majority of the stimulatory activity which was purified about 32-fold compared to native LFF. In contrast, addition of follicular fluid recovered from small porcine follicles inhibited FSH induction of LH/hCG binding sites and progesterone secretion. It can be concluded, that maturation of granulosa cells from small follicles may be enhanced by protein(s) present in LFF, but not in fluid recovered from less mature follicles.