Corrado Pedrazzani - Academia.edu (original) (raw)

Papers by Corrado Pedrazzani

Journal of Gastrointestinal Surgery, Aug 3, 2020

Journal of Clinical Oncology, Mar 1, 2007

Scandinavian Journal of Gastroenterology, 2008

1. Scand J Gastroenterol. 2008;43(6):765-6. Influence of age on soluble E-cadherin serum levels p... more 1. Scand J Gastroenterol. 2008;43(6):765-6. Influence of age on soluble E-cadherin serum levels prevents its utility as a disease marker in gastric cancer patients. Pedrazzani C, Caruso S, Corso G, Marrelli D, Neri A, Berardi A, Roviello F. ...

Annals of Surgical Oncology, Oct 8, 2009

We read with great interest the article by Kim and colleagues entitled ''Disparities in Gastric C... more We read with great interest the article by Kim and colleagues entitled ''Disparities in Gastric Cancer Outcomes among Asian Ethnicities in the USA.'' 1 In that study, notable differences in survival rates were observed among Asian patients with gastric cancer treated in Los Angeles. Several studies previously reported better outcome in Asian Americans when compared with other ethnicities, and these findings are confirmed in recent reports. 2-4 A peculiarity of this study is the demonstration of heterogeneous survival among Asian ethnicities. Geographic variability in prognosis of gastric cancer patients is well documented, as in Eastern series (primarily from Japan and Korea) higher survival rates were reported when compared with Western patients at the same stage of disease. 5-7 However, differences are also observed among European countries, and gastric cancer shows the largest variability in prognosis, when compared with other neoplasms, in a recent report of data from European cancer registries. 8 Epidemiological studies suggested a linear

European Journal of Surgical Oncology

Introduction: A series of T1N0Mx breast cancer was analyzed to identify features that may predict... more Introduction: A series of T1N0Mx breast cancer was analyzed to identify features that may predict outcome and influence treatment strategy. Methods: Data from 621 female patients with T1N0M0 breast carcinoma with a median follow-up of 7 years were analyzed. Results: The majority of the carcinomas were ductal type (78.9%). The greater part (52.7%) of primary lesion was classified as moderate grade (G2), while 20.9% as G1 and 14.8% G3. Peritumoral lymphatic/blood vessel invasion was detected in 81 (13%) tumorsThe strongest single feature influencing overall survival was LVI (p 0.0007). At multivariate analysis statistically significant prognostic features were also age 60 (p 0.008), premenopausal status (p 0.017), immunohistochemically detected positivity of estrogen hormone receptors (ER) (p 0.001), Mib1 (p 0.004) and BCL2 (p 0.050). Discussion: Identifying aggressive disease in T1N0M0 breast cancer would give valuable information for planning adjuvant therapy. Our analysis revealed that presence of LVI is the most reliable indicators of unfavourable outcome in T1N0M0 breast cancer. In addition, high Mib1 and BCL2 immunopositivity and low ER immunopositivity also have predictive value. The risk in women aged 60 years or younger was greater than that in women aged older than 60 years. No statistical difference was found between smaller and larger tumors (pT, p 0.46

Introduction: Elevation of E-cadherin serum levels has been reported in several tumors. The aim o... more Introduction: Elevation of E-cadherin serum levels has been reported in several tumors. The aim of this study was to evaluate the role of soluble E-cadherin in gastric cancer patients. Methods: Concentrations of soluble E-cadherin from 124 gastric cancer patients and 45 controls were analyzed. Results: All patients were prospectively studied with a median follow-up of 38 months. Soluble E-cadherin levels were not statistically different between gastric cancer patients and control subjects (p 0.060). A low sensitivity and specificity were estimated by the ROC curve offsetting its utility as a diagnostic marker. Soluble E-cadherin levels did not correlate with survival and no significant elevation was noticed according to the different clinicopathological characteristics, if not in relation to the age (p 0.008) or the presence of systemic metastases (p 0.044). Conversely, statistically significant differences in 5-year survival rates were observed according to the tumour site (p 0.013), the type of surgery (p 0.001), pT (p 0.001), pN (p 0.001) and M (p 0.001) categories. The correlation between soluble E cadherin and age was confirmed by linear regression analysis both for gastric cancer patients (p 0.015) and control subjects (p 0.030). Discussion: Soluble E-cadherins did not seem useful in gastric cancer patients. The correlation with age claims for age matched comparisons

European Journal of Surgical Oncology (EJSO), 2016

Early experience of robotic gastrectomy and comparison with laparoscopic gastrectomy for distal g... more Early experience of robotic gastrectomy and comparison with laparoscopic gastrectomy for distal gastric cancer

JAMA network open, Jun 4, 2024

Familial Cancer, Jan 17, 2009

About 90% of gastric cancer (GC) cases appear in a sporadic setting. Nonetheless, in high inciden... more About 90% of gastric cancer (GC) cases appear in a sporadic setting. Nonetheless, in high incidence areas high familial aggregation rates have been recently described. Microsatellite instability (MSI) is thought to be an important molecular phenotype both in sporadic GC and in tumors of the HNPCC spectrum. The aim of this study was to assess the frequency of MSI in GC with familial aggregation. Five quasimonomorphic mononucleotide repeats (BAT-26, BAT-25, NR-24, NR-21 and NR-27) were analyzed in 250 GC patients. Seventy-five patients (30%) had at least one-first-degree family member affected by GC and 63 patients (25.2%) showed MSI. The frequency of MSI was significantly higher in patients with a positive family history of GC (38.7%) compared to patients with other tumor types within the family (15.7%) or with a negative oncological familial history (21.9%, P = 0.004). Within cases with a positive familial oncological history, the MSI frequency in families with GC only was similar to the one observed in families with GC and colon cancer (P = 0.96). Nonetheless, in families with GC and lung cancer, the frequency of MSI was significantly lower (5.6%, P = 0.007). MSI occurs in GCs with familial aggregation. Similar MSI rates have been observed in GC patients with other family members affected by GC or colon cancer. The same does not occur in families with other members affected by lung cancer. Our data seem to suggest that familial aggregation for either GC alone or gastric and colon cancer share common etiological factors in contrast to families with gastric and lung cancers. Keywords Gastric cancer Á Family history Á Microsatellite instability C. Pedrazzani and G. Corso are contributed equally.

European Journal of Cancer, Feb 1, 2011

Mitogen-activated protein kinase (MAPK) cascade and phosphatidylinositol 3-kinase (PI3K) survival... more Mitogen-activated protein kinase (MAPK) cascade and phosphatidylinositol 3-kinase (PI3K) survival pathways are frequently activated in the progression of gastrointestinal malignancies. In this study, we aimed to determine the frequency of gene mutations in members of these pathways--Epithelial Growth Factor Receptor (EGFR), KRAS, BRAF, PIK3CA and MLK3 in a series of 63 gastric carcinomas with high levels of microsatellite instability (MSI). Gene mutation analysis was performed by PCR amplification followed by direct sequencing. In selected tumour cases, EGFR expression was evaluated by immunohistochemistry. Association studies between molecular data and clinicopathologic characteristics were performed. Mutations in EGFR (3'-untranslated region [UTR] polyA repeat), KRAS, PIK3CA and MLK3 genes occurred in 30 (47.6%), 11 (17.5%), 9 (14.3%) and 2 (3.2%) of the MSI gastric cancer (GC) cases, respectively. No BRAF or EGFR hotspot mutations were identified. Overall, mutations in at least one of these genes were found in 55.6% (35/63) of gastric carcinomas. From those mutant cases 40.0% (14/35) of them had concomitant gene mutations, always involving EGFR polyA deletions. Interestingly, we observed significant associations between oncogenic mutations and female gender (p = 0.046) old age of diagnosis (p = 0.001) and intestinal subtype (p = 0.043). Our results show that MSI gastric carcinoma frequently shows activation of EGFR-MAPK and PI3K pathways. Within all alterations found, deletions of the A13 repeats of EGFR were common, suggesting this molecular event as an important biomarker for stratification of GC patients for treatment with EGFR inhibitors.

World Journal of Surgery, Mar 18, 2010

We thank Dr. Biondi and colleagues for their comments and questions, which allow us further clari... more We thank Dr. Biondi and colleagues for their comments and questions, which allow us further clarification about this controversial issue [1]. The lymph node ratio has been extensively studied in gastric cancer, and several articles reported it as a more accurate prognostic factor with respect to the current UICC and JGCA classifications. However, most of these studies were performed at institutions with fair or good experience with gastric cancer surgery and pathologic assessment. In a recent report from the Italian Research Group for Gastric Cancer [2], we confirmed these results in a large cohort of gastric cancer patients operated on in six specialized Italian centers, where the quality of lymph node dissection, nodal retrieval, and pathologic assessment had been standardized for years. Indeed, patients treated by ''limited'' or ''extended'' lymphadenectomy had a median of 11 and 30 examined lymph nodes, respectively, and rarely fewer than 10. In patients with such numbers of removed lymph nodes, the lymph node ratio acts very well. The present study aimed to evaluate if the lymph node ratio is applicable and accurate similarly to the data from the Latvian Oncology Center, which resembles what may happen in nondedicated institutions around the world. In this series of 526 consecutive patients operated on between

Annals of Surgical Oncology, Apr 12, 2017

Background. The purpose of this retrospective study was to evaluate the incidence and prognostic ... more Background. The purpose of this retrospective study was to evaluate the incidence and prognostic value of metastases to ''posterior'' (8p, 12b/p, 13) and para-aortic lymph nodes in a large cohort of Western patients submitted to D2 plus lymphadenectomy. Methods. Removal of ''posterior'' nodes was performed in 743 patients, and para-aortic lymphadenectomy in a subgroup of 390 patients. After lymph node mapping and retrieval on the fresh specimen, a median number of 41 total lymph nodes were analyzed. The median follow-up period was 37 months for the entire series and 68 months for survivors. Results. Of 743 included patients, 23 (3.1%) had metastases in station 8p, 12 (1.6%) in station 12b/p, and 19 (2.6%) in station 13. On the whole, 47 of 743 patients (6.3%) had positive ''posterior'' nodes. Para-aortic metastases were present in 42 of 390 patients (10.8%). Metastases to ''posterior'' stations were significantly related to depth of invasion, number of positive nodes, and surgical radicality. Distal tumors showed higher trend to metastasize to ''posterior'' nodes than upper third, whereas for para-aortic metastases it was the reverse. 5-year survival in patients with positivity to ''posterior'' nodes was 17%, with no significant difference according to 8p, 12b/p, and 13 stations; long-term outcome was overlapping to pN3b stage. 5-year survival in para-aortic positive cases was 11%, and a trend to better outcome was observed in proximal tumors. Conclusions. Although metastases to ''posterior'' and para-aortic nodes are expression of an advanced nodal stage, not negligible survival rates are observed in subgroups of patients.

Journal of Gastrointestinal Surgery, Aug 7, 2009

Purpose X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins ... more Purpose X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the basexcision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the-77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the-77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls. Methods GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1-77T>C was detected using restriction enzyme BsrB I and analyzed in a 3% agarose gel. Results The-77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p=0.023) with an odds ratio of 1.65 (95% confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p=0.043) between the-77T>C polymorphism and GC cases with familial lung cancer aggregation. Conclusions Our results suggest that the XRCC1-77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.

Journal of Gastrointestinal Surgery, Aug 3, 2020

Journal of Clinical Oncology, Mar 1, 2007

Scandinavian Journal of Gastroenterology, 2008

1. Scand J Gastroenterol. 2008;43(6):765-6. Influence of age on soluble E-cadherin serum levels p... more 1. Scand J Gastroenterol. 2008;43(6):765-6. Influence of age on soluble E-cadherin serum levels prevents its utility as a disease marker in gastric cancer patients. Pedrazzani C, Caruso S, Corso G, Marrelli D, Neri A, Berardi A, Roviello F. ...

Annals of Surgical Oncology, Oct 8, 2009

We read with great interest the article by Kim and colleagues entitled ''Disparities in Gastric C... more We read with great interest the article by Kim and colleagues entitled ''Disparities in Gastric Cancer Outcomes among Asian Ethnicities in the USA.'' 1 In that study, notable differences in survival rates were observed among Asian patients with gastric cancer treated in Los Angeles. Several studies previously reported better outcome in Asian Americans when compared with other ethnicities, and these findings are confirmed in recent reports. 2-4 A peculiarity of this study is the demonstration of heterogeneous survival among Asian ethnicities. Geographic variability in prognosis of gastric cancer patients is well documented, as in Eastern series (primarily from Japan and Korea) higher survival rates were reported when compared with Western patients at the same stage of disease. 5-7 However, differences are also observed among European countries, and gastric cancer shows the largest variability in prognosis, when compared with other neoplasms, in a recent report of data from European cancer registries. 8 Epidemiological studies suggested a linear

European Journal of Surgical Oncology

Introduction: A series of T1N0Mx breast cancer was analyzed to identify features that may predict... more Introduction: A series of T1N0Mx breast cancer was analyzed to identify features that may predict outcome and influence treatment strategy. Methods: Data from 621 female patients with T1N0M0 breast carcinoma with a median follow-up of 7 years were analyzed. Results: The majority of the carcinomas were ductal type (78.9%). The greater part (52.7%) of primary lesion was classified as moderate grade (G2), while 20.9% as G1 and 14.8% G3. Peritumoral lymphatic/blood vessel invasion was detected in 81 (13%) tumorsThe strongest single feature influencing overall survival was LVI (p 0.0007). At multivariate analysis statistically significant prognostic features were also age 60 (p 0.008), premenopausal status (p 0.017), immunohistochemically detected positivity of estrogen hormone receptors (ER) (p 0.001), Mib1 (p 0.004) and BCL2 (p 0.050). Discussion: Identifying aggressive disease in T1N0M0 breast cancer would give valuable information for planning adjuvant therapy. Our analysis revealed that presence of LVI is the most reliable indicators of unfavourable outcome in T1N0M0 breast cancer. In addition, high Mib1 and BCL2 immunopositivity and low ER immunopositivity also have predictive value. The risk in women aged 60 years or younger was greater than that in women aged older than 60 years. No statistical difference was found between smaller and larger tumors (pT, p 0.46

Introduction: Elevation of E-cadherin serum levels has been reported in several tumors. The aim o... more Introduction: Elevation of E-cadherin serum levels has been reported in several tumors. The aim of this study was to evaluate the role of soluble E-cadherin in gastric cancer patients. Methods: Concentrations of soluble E-cadherin from 124 gastric cancer patients and 45 controls were analyzed. Results: All patients were prospectively studied with a median follow-up of 38 months. Soluble E-cadherin levels were not statistically different between gastric cancer patients and control subjects (p 0.060). A low sensitivity and specificity were estimated by the ROC curve offsetting its utility as a diagnostic marker. Soluble E-cadherin levels did not correlate with survival and no significant elevation was noticed according to the different clinicopathological characteristics, if not in relation to the age (p 0.008) or the presence of systemic metastases (p 0.044). Conversely, statistically significant differences in 5-year survival rates were observed according to the tumour site (p 0.013), the type of surgery (p 0.001), pT (p 0.001), pN (p 0.001) and M (p 0.001) categories. The correlation between soluble E cadherin and age was confirmed by linear regression analysis both for gastric cancer patients (p 0.015) and control subjects (p 0.030). Discussion: Soluble E-cadherins did not seem useful in gastric cancer patients. The correlation with age claims for age matched comparisons

European Journal of Surgical Oncology (EJSO), 2016

Early experience of robotic gastrectomy and comparison with laparoscopic gastrectomy for distal g... more Early experience of robotic gastrectomy and comparison with laparoscopic gastrectomy for distal gastric cancer

JAMA network open, Jun 4, 2024

Familial Cancer, Jan 17, 2009

About 90% of gastric cancer (GC) cases appear in a sporadic setting. Nonetheless, in high inciden... more About 90% of gastric cancer (GC) cases appear in a sporadic setting. Nonetheless, in high incidence areas high familial aggregation rates have been recently described. Microsatellite instability (MSI) is thought to be an important molecular phenotype both in sporadic GC and in tumors of the HNPCC spectrum. The aim of this study was to assess the frequency of MSI in GC with familial aggregation. Five quasimonomorphic mononucleotide repeats (BAT-26, BAT-25, NR-24, NR-21 and NR-27) were analyzed in 250 GC patients. Seventy-five patients (30%) had at least one-first-degree family member affected by GC and 63 patients (25.2%) showed MSI. The frequency of MSI was significantly higher in patients with a positive family history of GC (38.7%) compared to patients with other tumor types within the family (15.7%) or with a negative oncological familial history (21.9%, P = 0.004). Within cases with a positive familial oncological history, the MSI frequency in families with GC only was similar to the one observed in families with GC and colon cancer (P = 0.96). Nonetheless, in families with GC and lung cancer, the frequency of MSI was significantly lower (5.6%, P = 0.007). MSI occurs in GCs with familial aggregation. Similar MSI rates have been observed in GC patients with other family members affected by GC or colon cancer. The same does not occur in families with other members affected by lung cancer. Our data seem to suggest that familial aggregation for either GC alone or gastric and colon cancer share common etiological factors in contrast to families with gastric and lung cancers. Keywords Gastric cancer Á Family history Á Microsatellite instability C. Pedrazzani and G. Corso are contributed equally.

European Journal of Cancer, Feb 1, 2011

Mitogen-activated protein kinase (MAPK) cascade and phosphatidylinositol 3-kinase (PI3K) survival... more Mitogen-activated protein kinase (MAPK) cascade and phosphatidylinositol 3-kinase (PI3K) survival pathways are frequently activated in the progression of gastrointestinal malignancies. In this study, we aimed to determine the frequency of gene mutations in members of these pathways--Epithelial Growth Factor Receptor (EGFR), KRAS, BRAF, PIK3CA and MLK3 in a series of 63 gastric carcinomas with high levels of microsatellite instability (MSI). Gene mutation analysis was performed by PCR amplification followed by direct sequencing. In selected tumour cases, EGFR expression was evaluated by immunohistochemistry. Association studies between molecular data and clinicopathologic characteristics were performed. Mutations in EGFR (3'-untranslated region [UTR] polyA repeat), KRAS, PIK3CA and MLK3 genes occurred in 30 (47.6%), 11 (17.5%), 9 (14.3%) and 2 (3.2%) of the MSI gastric cancer (GC) cases, respectively. No BRAF or EGFR hotspot mutations were identified. Overall, mutations in at least one of these genes were found in 55.6% (35/63) of gastric carcinomas. From those mutant cases 40.0% (14/35) of them had concomitant gene mutations, always involving EGFR polyA deletions. Interestingly, we observed significant associations between oncogenic mutations and female gender (p = 0.046) old age of diagnosis (p = 0.001) and intestinal subtype (p = 0.043). Our results show that MSI gastric carcinoma frequently shows activation of EGFR-MAPK and PI3K pathways. Within all alterations found, deletions of the A13 repeats of EGFR were common, suggesting this molecular event as an important biomarker for stratification of GC patients for treatment with EGFR inhibitors.

World Journal of Surgery, Mar 18, 2010

We thank Dr. Biondi and colleagues for their comments and questions, which allow us further clari... more We thank Dr. Biondi and colleagues for their comments and questions, which allow us further clarification about this controversial issue [1]. The lymph node ratio has been extensively studied in gastric cancer, and several articles reported it as a more accurate prognostic factor with respect to the current UICC and JGCA classifications. However, most of these studies were performed at institutions with fair or good experience with gastric cancer surgery and pathologic assessment. In a recent report from the Italian Research Group for Gastric Cancer [2], we confirmed these results in a large cohort of gastric cancer patients operated on in six specialized Italian centers, where the quality of lymph node dissection, nodal retrieval, and pathologic assessment had been standardized for years. Indeed, patients treated by ''limited'' or ''extended'' lymphadenectomy had a median of 11 and 30 examined lymph nodes, respectively, and rarely fewer than 10. In patients with such numbers of removed lymph nodes, the lymph node ratio acts very well. The present study aimed to evaluate if the lymph node ratio is applicable and accurate similarly to the data from the Latvian Oncology Center, which resembles what may happen in nondedicated institutions around the world. In this series of 526 consecutive patients operated on between

Annals of Surgical Oncology, Apr 12, 2017

Background. The purpose of this retrospective study was to evaluate the incidence and prognostic ... more Background. The purpose of this retrospective study was to evaluate the incidence and prognostic value of metastases to ''posterior'' (8p, 12b/p, 13) and para-aortic lymph nodes in a large cohort of Western patients submitted to D2 plus lymphadenectomy. Methods. Removal of ''posterior'' nodes was performed in 743 patients, and para-aortic lymphadenectomy in a subgroup of 390 patients. After lymph node mapping and retrieval on the fresh specimen, a median number of 41 total lymph nodes were analyzed. The median follow-up period was 37 months for the entire series and 68 months for survivors. Results. Of 743 included patients, 23 (3.1%) had metastases in station 8p, 12 (1.6%) in station 12b/p, and 19 (2.6%) in station 13. On the whole, 47 of 743 patients (6.3%) had positive ''posterior'' nodes. Para-aortic metastases were present in 42 of 390 patients (10.8%). Metastases to ''posterior'' stations were significantly related to depth of invasion, number of positive nodes, and surgical radicality. Distal tumors showed higher trend to metastasize to ''posterior'' nodes than upper third, whereas for para-aortic metastases it was the reverse. 5-year survival in patients with positivity to ''posterior'' nodes was 17%, with no significant difference according to 8p, 12b/p, and 13 stations; long-term outcome was overlapping to pN3b stage. 5-year survival in para-aortic positive cases was 11%, and a trend to better outcome was observed in proximal tumors. Conclusions. Although metastases to ''posterior'' and para-aortic nodes are expression of an advanced nodal stage, not negligible survival rates are observed in subgroups of patients.

Journal of Gastrointestinal Surgery, Aug 7, 2009

Purpose X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins ... more Purpose X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the basexcision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the-77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the-77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls. Methods GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1-77T>C was detected using restriction enzyme BsrB I and analyzed in a 3% agarose gel. Results The-77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p=0.023) with an odds ratio of 1.65 (95% confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p=0.043) between the-77T>C polymorphism and GC cases with familial lung cancer aggregation. Conclusions Our results suggest that the XRCC1-77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.