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Papers by Eric Craft

Research paper thumbnail of Employing Immersion Theory as a Public Affairs Instructional Tool in Frontier Conflict Regions

Research paper thumbnail of High-throughput two-dimensional root system phenotyping platform facilitates genetic analysis of root growth and development

Plant, Cell & Environment, 2013

Research paper thumbnail of Transport properties of members of the ZIP family in plants and their role in Zn and Mn homeostasis

Journal of Experimental Botany, 2013

Research paper thumbnail of OPT3 Is a Phloem-Specific Iron Transporter That Is Essential for Systemic Iron Signaling and Redistribution of Iron and Cadmium in Arabidopsis

Research paper thumbnail of Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation

Research paper thumbnail of GEOCHEM-EZ: a chemical speciation program with greater power and flexibility

Research paper thumbnail of Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation

New Phytologist, 2012

• In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the z... more • In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. • We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. • Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. • In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

Research paper thumbnail of COPT6 Is a Plasma Membrane Transporter That Functions in Copper Homeostasis in Arabidopsis and Is a Novel Target of SQUAMOSA Promoter-binding Protein-like 7

Journal of Biological Chemistry, 2012

Research paper thumbnail of Development of a Novel Aluminum Tolerance Phenotyping Platform Used for Comparisons of Cereal Aluminum Tolerance and Investigations into Rice Aluminum Tolerance Mechanisms

Research paper thumbnail of Evolving technologies for growing, imaging, and analyzing 3D root system architecture of crop plants

Journal of Integrative Plant Biology, 2015

A plant&a... more A plant's ability to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, can be strongly influenced by root system architecture, the 3-dimensional distribution of the different root types in the soil. The ability to image, track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying root system architecture. We have designed a hydroponic growth system which retains the 3-dimensional root system architecture (RSA) of the plant root system, while allowing for aeration, solution replenishment, and the imposition of nutrient treatments, as well as high quality imaging of the root system. The simplicity and flexibility of the system allows for modifications tailored the root system architecture of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity (detection of fine roots and other root details), higher efficiency, and a broad array of growing conditions for plants that more closely mimic those found under field conditions.

Research paper thumbnail of Employing Immersion Theory as a Public Affairs Instructional Tool in Frontier Conflict Regions

Research paper thumbnail of High-throughput two-dimensional root system phenotyping platform facilitates genetic analysis of root growth and development

Plant, Cell & Environment, 2013

Research paper thumbnail of Transport properties of members of the ZIP family in plants and their role in Zn and Mn homeostasis

Journal of Experimental Botany, 2013

Research paper thumbnail of OPT3 Is a Phloem-Specific Iron Transporter That Is Essential for Systemic Iron Signaling and Redistribution of Iron and Cadmium in Arabidopsis

Research paper thumbnail of Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation

Research paper thumbnail of GEOCHEM-EZ: a chemical speciation program with greater power and flexibility

Research paper thumbnail of Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation

New Phytologist, 2012

• In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the z... more • In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. • We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. • Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. • In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

Research paper thumbnail of COPT6 Is a Plasma Membrane Transporter That Functions in Copper Homeostasis in Arabidopsis and Is a Novel Target of SQUAMOSA Promoter-binding Protein-like 7

Journal of Biological Chemistry, 2012

Research paper thumbnail of Development of a Novel Aluminum Tolerance Phenotyping Platform Used for Comparisons of Cereal Aluminum Tolerance and Investigations into Rice Aluminum Tolerance Mechanisms

Research paper thumbnail of Evolving technologies for growing, imaging, and analyzing 3D root system architecture of crop plants

Journal of Integrative Plant Biology, 2015

A plant&a... more A plant's ability to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, can be strongly influenced by root system architecture, the 3-dimensional distribution of the different root types in the soil. The ability to image, track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying root system architecture. We have designed a hydroponic growth system which retains the 3-dimensional root system architecture (RSA) of the plant root system, while allowing for aeration, solution replenishment, and the imposition of nutrient treatments, as well as high quality imaging of the root system. The simplicity and flexibility of the system allows for modifications tailored the root system architecture of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity (detection of fine roots and other root details), higher efficiency, and a broad array of growing conditions for plants that more closely mimic those found under field conditions.

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