Cristian Aguilera - Academia.edu (original) (raw)

Uploads

Papers by Cristian Aguilera

Research paper thumbnail of Interactive effects of diet and temperature on reproductive conditioning of Argopecten purpuratus broodstock

Aquaculture, 2000

Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile ... more Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile were conditioned at two temperatures (16 and 208C) and three different diets (mixture of pure microalgae, microalgae supplemented with lipids and microalgae supplemented with carbohydrates). The food, equivalent to 3% daily of the animal dry weight was supplied continuously by a peristaltic pump. The rates of different physiological processes were measured on 18 scallops (three replicates per temperature / diet combination) during the third week of conditioning. Nine scallops came from the group conditioned at 168C and other nine from the group conditioned at 208C (each scallop from a different tank). As in the conditioning experiment, physiological measurements were made in controlled temperature rooms using the same experimental diets. Argopecten purpuratus did not show significant differences in clearance rate between 16 and 208C when fed with pure microalgae and microalgae 1 lipids, showing a well known capability of bivalves to acclimate their physiological rates within a certain range of temperature. On the other hand a clear effect of the composition of the diet on the clearance rate was observed. When microalgae were supplemented with a lipid emulsion, clearance rate was highly stimulated, showing values significantly higher in comparison with either pure microalgae, or a mixed diet of microalgae 1 carbohydrate. These highest feeding rates can be related with the presence of essential fatty acids in the diet, which are considered as very important compounds during the gametogenesis of invertebrates as well as during the development of ova into normal larvae. Thus A. purpuratus can actively regulate clearance rate and does not simply switch between feeding and non-feeding states. The data also suggest the presence of chemical receptors at the level of the gills and / or labial palps, which seem able to detect specific nutritive compounds present in the diet. Absorption efficiency was independent of temperature and was higher with pure microalgae and with microalgae 1 lipids. The lowest efficiencies were recorded with the diet *Corresponding supplemented with carbohydrates. The energy expended in oxygen uptake and ammonia excretion was very similar in the different experimental diets and temperatures. Scope for growth in A. purpuratus appears mainly affected by the diet and not by temperature. While the lower SFG seems to be associated with diets composed of pure microalgae and microalgae 1 carbohydrates, the highest values being found for a diet rich in lipids. The data were in agreement with the reproductive conditioning of A. purpuratus, where the highest percentage of ripe scallops occurred in individuals fed with a diet of microalgae supplemented with lipids at both temperatures. Similarly the highest larval survival rate was obtained from gametes released by scallops conditioned with the diet containing lipids.

Research paper thumbnail of Effect of diet and temperature upon muscle metabolic capacities and biochemical composition of gonad and muscle in Argopecten purpuratus Lamarck 1819

Journal of Experimental Marine Biology and Ecology, 2000

Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed th... more Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed three different diets (microalgae, microalgae mixed with lipids and microalgae mixed with carbohydrates) to examine changes in the biochemical composition of gonad and muscle as well as muscle metabolic capacities. During one experiment, scallops were fed at 3% of their dry mass per day whereas during a second experiment, they were fed at 6% of their dry mass per day. During both experiments, total gonadal levels of lipids and protein increased markedly during conditioning with the two mixed diets at 168C. These increases were less pronounced at 208C. Carbohydrate gonadal levels only increased during the second experiment at both temperatures and with the three diets. Of the major biochemical components of the adductor muscle, carbohydrate levels changed most during conditioning. Whereas muscle protein levels increased slightly with gonadal maturation, carbohydrate levels dropped considerably. Despite the marked drop in the levels of glycolytic substrates, only the activities of octopine dehydrogenase in the adductor muscle of the scallops conditioned at 168C consistently decreased. Muscle levels of glycogen phosphorylase were higher in mature than in recently spawned (control) scallops, suggesting a role in the transfer of glucose equivalents from the adductor muscle to other tissues.

Research paper thumbnail of Interactive effects of diet and temperature on the scope for growth of the scallop Argopecten purpuratus during reproductive conditioning

Journal of Experimental Marine Biology and Ecology, 2000

Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile ... more Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile were conditioned at two temperatures (16 and 208C) and three different diets (mixture of pure microalgae, microalgae supplemented with lipids and microalgae supplemented with carbohydrates). The food, equivalent to 3% daily of the animal dry weight was supplied continuously by a peristaltic pump. The rates of different physiological processes were measured on 18 scallops (three replicates per temperature / diet combination) during the third week of conditioning. Nine scallops came from the group conditioned at 168C and other nine from the group conditioned at 208C (each scallop from a different tank). As in the conditioning experiment, physiological measurements were made in controlled temperature rooms using the same experimental diets. Argopecten purpuratus did not show significant differences in clearance rate between 16 and 208C when fed with pure microalgae and microalgae 1 lipids, showing a well known capability of bivalves to acclimate their physiological rates within a certain range of temperature. On the other hand a clear effect of the composition of the diet on the clearance rate was observed. When microalgae were supplemented with a lipid emulsion, clearance rate was highly stimulated, showing values significantly higher in comparison with either pure microalgae, or a mixed diet of microalgae 1 carbohydrate. These highest feeding rates can be related with the presence of essential fatty acids in the diet, which are considered as very important compounds during the gametogenesis of invertebrates as well as during the development of ova into normal larvae. Thus A. purpuratus can actively regulate clearance rate and does not simply switch between feeding and non-feeding states. The data also suggest the presence of chemical receptors at the level of the gills and / or labial palps, which seem able to detect specific nutritive compounds present in the diet. Absorption efficiency was independent of temperature and was higher with pure microalgae and with microalgae 1 lipids. The lowest efficiencies were recorded with the diet *Corresponding supplemented with carbohydrates. The energy expended in oxygen uptake and ammonia excretion was very similar in the different experimental diets and temperatures. Scope for growth in A. purpuratus appears mainly affected by the diet and not by temperature. While the lower SFG seems to be associated with diets composed of pure microalgae and microalgae 1 carbohydrates, the highest values being found for a diet rich in lipids. The data were in agreement with the reproductive conditioning of A. purpuratus, where the highest percentage of ripe scallops occurred in individuals fed with a diet of microalgae supplemented with lipids at both temperatures. Similarly the highest larval survival rate was obtained from gametes released by scallops conditioned with the diet containing lipids.

Research paper thumbnail of violacion franca

Journal of Experimental Marine Biology and Ecology, 2000

Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed th... more Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed three different diets (microalgae, microalgae mixed with lipids and microalgae mixed with carbohydrates) to examine changes in the biochemical composition of gonad and muscle as well as muscle metabolic capacities. During one experiment, scallops were fed at 3% of their dry mass per day whereas during a second experiment, they were fed at 6% of their dry mass per day. During both experiments, total gonadal levels of lipids and protein increased markedly during conditioning with the two mixed diets at 168C. These increases were less pronounced at 208C. Carbohydrate gonadal levels only increased during the second experiment at both temperatures and with the three diets. Of the major biochemical components of the adductor muscle, carbohydrate levels changed most during conditioning. Whereas muscle protein levels increased slightly with gonadal maturation, carbohydrate levels dropped considerably. Despite the marked drop in the levels of glycolytic substrates, only the activities of octopine dehydrogenase in the adductor muscle of the scallops conditioned at 168C consistently decreased. Muscle levels of glycogen phosphorylase were higher in mature than in recently spawned (control) scallops, suggesting a role in the transfer of glucose equivalents from the adductor muscle to other tissues.

Research paper thumbnail of Interactive effects of diet and temperature on reproductive conditioning of Argopecten purpuratus broodstock

Aquaculture, 2000

Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile ... more Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile were conditioned at two temperatures (16 and 208C) and three different diets (mixture of pure microalgae, microalgae supplemented with lipids and microalgae supplemented with carbohydrates). The food, equivalent to 3% daily of the animal dry weight was supplied continuously by a peristaltic pump. The rates of different physiological processes were measured on 18 scallops (three replicates per temperature / diet combination) during the third week of conditioning. Nine scallops came from the group conditioned at 168C and other nine from the group conditioned at 208C (each scallop from a different tank). As in the conditioning experiment, physiological measurements were made in controlled temperature rooms using the same experimental diets. Argopecten purpuratus did not show significant differences in clearance rate between 16 and 208C when fed with pure microalgae and microalgae 1 lipids, showing a well known capability of bivalves to acclimate their physiological rates within a certain range of temperature. On the other hand a clear effect of the composition of the diet on the clearance rate was observed. When microalgae were supplemented with a lipid emulsion, clearance rate was highly stimulated, showing values significantly higher in comparison with either pure microalgae, or a mixed diet of microalgae 1 carbohydrate. These highest feeding rates can be related with the presence of essential fatty acids in the diet, which are considered as very important compounds during the gametogenesis of invertebrates as well as during the development of ova into normal larvae. Thus A. purpuratus can actively regulate clearance rate and does not simply switch between feeding and non-feeding states. The data also suggest the presence of chemical receptors at the level of the gills and / or labial palps, which seem able to detect specific nutritive compounds present in the diet. Absorption efficiency was independent of temperature and was higher with pure microalgae and with microalgae 1 lipids. The lowest efficiencies were recorded with the diet *Corresponding supplemented with carbohydrates. The energy expended in oxygen uptake and ammonia excretion was very similar in the different experimental diets and temperatures. Scope for growth in A. purpuratus appears mainly affected by the diet and not by temperature. While the lower SFG seems to be associated with diets composed of pure microalgae and microalgae 1 carbohydrates, the highest values being found for a diet rich in lipids. The data were in agreement with the reproductive conditioning of A. purpuratus, where the highest percentage of ripe scallops occurred in individuals fed with a diet of microalgae supplemented with lipids at both temperatures. Similarly the highest larval survival rate was obtained from gametes released by scallops conditioned with the diet containing lipids.

Research paper thumbnail of Effect of diet and temperature upon muscle metabolic capacities and biochemical composition of gonad and muscle in Argopecten purpuratus Lamarck 1819

Journal of Experimental Marine Biology and Ecology, 2000

Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed th... more Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed three different diets (microalgae, microalgae mixed with lipids and microalgae mixed with carbohydrates) to examine changes in the biochemical composition of gonad and muscle as well as muscle metabolic capacities. During one experiment, scallops were fed at 3% of their dry mass per day whereas during a second experiment, they were fed at 6% of their dry mass per day. During both experiments, total gonadal levels of lipids and protein increased markedly during conditioning with the two mixed diets at 168C. These increases were less pronounced at 208C. Carbohydrate gonadal levels only increased during the second experiment at both temperatures and with the three diets. Of the major biochemical components of the adductor muscle, carbohydrate levels changed most during conditioning. Whereas muscle protein levels increased slightly with gonadal maturation, carbohydrate levels dropped considerably. Despite the marked drop in the levels of glycolytic substrates, only the activities of octopine dehydrogenase in the adductor muscle of the scallops conditioned at 168C consistently decreased. Muscle levels of glycogen phosphorylase were higher in mature than in recently spawned (control) scallops, suggesting a role in the transfer of glucose equivalents from the adductor muscle to other tissues.

Research paper thumbnail of Interactive effects of diet and temperature on the scope for growth of the scallop Argopecten purpuratus during reproductive conditioning

Journal of Experimental Marine Biology and Ecology, 2000

Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile ... more Mature individuals of Argopecten purpuratus from suspended culture in Tongoy Bay, northern Chile were conditioned at two temperatures (16 and 208C) and three different diets (mixture of pure microalgae, microalgae supplemented with lipids and microalgae supplemented with carbohydrates). The food, equivalent to 3% daily of the animal dry weight was supplied continuously by a peristaltic pump. The rates of different physiological processes were measured on 18 scallops (three replicates per temperature / diet combination) during the third week of conditioning. Nine scallops came from the group conditioned at 168C and other nine from the group conditioned at 208C (each scallop from a different tank). As in the conditioning experiment, physiological measurements were made in controlled temperature rooms using the same experimental diets. Argopecten purpuratus did not show significant differences in clearance rate between 16 and 208C when fed with pure microalgae and microalgae 1 lipids, showing a well known capability of bivalves to acclimate their physiological rates within a certain range of temperature. On the other hand a clear effect of the composition of the diet on the clearance rate was observed. When microalgae were supplemented with a lipid emulsion, clearance rate was highly stimulated, showing values significantly higher in comparison with either pure microalgae, or a mixed diet of microalgae 1 carbohydrate. These highest feeding rates can be related with the presence of essential fatty acids in the diet, which are considered as very important compounds during the gametogenesis of invertebrates as well as during the development of ova into normal larvae. Thus A. purpuratus can actively regulate clearance rate and does not simply switch between feeding and non-feeding states. The data also suggest the presence of chemical receptors at the level of the gills and / or labial palps, which seem able to detect specific nutritive compounds present in the diet. Absorption efficiency was independent of temperature and was higher with pure microalgae and with microalgae 1 lipids. The lowest efficiencies were recorded with the diet *Corresponding supplemented with carbohydrates. The energy expended in oxygen uptake and ammonia excretion was very similar in the different experimental diets and temperatures. Scope for growth in A. purpuratus appears mainly affected by the diet and not by temperature. While the lower SFG seems to be associated with diets composed of pure microalgae and microalgae 1 carbohydrates, the highest values being found for a diet rich in lipids. The data were in agreement with the reproductive conditioning of A. purpuratus, where the highest percentage of ripe scallops occurred in individuals fed with a diet of microalgae supplemented with lipids at both temperatures. Similarly the highest larval survival rate was obtained from gametes released by scallops conditioned with the diet containing lipids.

Research paper thumbnail of violacion franca

Journal of Experimental Marine Biology and Ecology, 2000

Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed th... more Recently spawned Argopecten purpuratus broodstock were conditioned at two temperatures and fed three different diets (microalgae, microalgae mixed with lipids and microalgae mixed with carbohydrates) to examine changes in the biochemical composition of gonad and muscle as well as muscle metabolic capacities. During one experiment, scallops were fed at 3% of their dry mass per day whereas during a second experiment, they were fed at 6% of their dry mass per day. During both experiments, total gonadal levels of lipids and protein increased markedly during conditioning with the two mixed diets at 168C. These increases were less pronounced at 208C. Carbohydrate gonadal levels only increased during the second experiment at both temperatures and with the three diets. Of the major biochemical components of the adductor muscle, carbohydrate levels changed most during conditioning. Whereas muscle protein levels increased slightly with gonadal maturation, carbohydrate levels dropped considerably. Despite the marked drop in the levels of glycolytic substrates, only the activities of octopine dehydrogenase in the adductor muscle of the scallops conditioned at 168C consistently decreased. Muscle levels of glycogen phosphorylase were higher in mature than in recently spawned (control) scallops, suggesting a role in the transfer of glucose equivalents from the adductor muscle to other tissues.