Cristiano Lerche - Academia.edu (original) (raw)
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Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine... more Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine-disruption testing. To establish a nondestructive swab sampling method, VTG induction in the epidermis of Cypriniformes and Perciformes species was investigated. Both VTG and estrogen receptor genes are expressed in epidermal cells. Immunoaffinity and mass fingerprint analyses show induction of identical VTG peptides in liver and epidermis. Induction of VTG by estradiol (E2) and bisphenol A (BPA) in the epidermis was quantified with homolog enzyme-linked immunosorbent assays. Initial values in juveniles and males were below 1 ng VTG/mL extraction buffer. Exposure to E2 led to values between 200 ng/mL and 4600 ng/mL in cyprinids and between 10 ng/mL and 81 ng/mL in perciforms. Exposure to BPA increased VTG amounts to 250 ng/mL in fathead minnows, 1360 ng/mL in goldfish, 100 ng/mL in zebrafish, and 12 ng/mL in bluegills. Serum VTG contents demonstrated a similar dose–response pattern in t...
This study focuses on some aspects of the natural history of the cave heteropteran Zelurus travas... more This study focuses on some aspects of the natural history of the cave heteropteran Zelurus travassosi Costa-Lima, 1940, which is found in several caves from the Ribeira Valley – state of Sao Paulo. The study includes (1) the population dynamics, using mark-recapture methodology and estimation of the population number in Parque Estadual Intervales; (2) the life cycle, by means of rearing the different stages in the laboratory; and (3) morphometrics of each stage. In addition, observations about predatory behavior, reproductive behavior and spatial distribution within the caves have been made. Grant FAPESP # 00/03384-4
Environmental toxicology and chemistry / SETAC, Dec 6, 2016
Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine... more Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine disruption testing. In order to establish a nondestructive swab sampling method, VTG induction in the epidermis of Cypriniformes and Perciformes species has been investigated. Both vitellogenin and estrogen receptor genes are expressed in epidermal cells. Immunoaffinity and mass fingerprint analyses show induction of identical VTG peptides in liver and epidermis. Vitellogenin induction by estradiol (E2) and bisphenol A (BPA) in the epidermis was quantified with homolog enzyme-linked immunosorbent assays (ELISA). Initial values in juveniles and males were below 1 ng VTG/mL extraction buffer. Exposure to E2 led to values between 200 ng/mL and 4600 ng/mL in cyprinids and between 10 ng/mL and 81 ng/mL in perciforms. Exposure to BPA increased VTG amounts to 250 ng/mL in fathead minnows, 1360 ng/mL in goldfish, 100 ng/mL in zebrafish and 12 ng/mL in bluegills. VTG contents in the serum demons...
Journal of Experimental Zoology Part B-molecular and Developmental Evolution, 2010
The first studies concerning the embryonic development of harvestmen started in the late 19th cen... more The first studies concerning the embryonic development of harvestmen started in the late 19th century, and focused mostly on holarctic species, and only three species of the suborder Laniatores (the largest, among the four suborders considered presently) were studied. Moreover, the last studies on embryology of harvestmen were made during the late 1970s. This study focused on the embryonic development of Ampheres leucopheus (Gonyleptidae, Caelopyginae) and Iporangaia pustulosa (Gonyleptidae, Progonyleptoidellinae). The embryonic development was followed in the field, by taking daily photographs of different eggs during about 2 months. When laid, eggs of A. leucopheus and I. pustulosa have approximately 1.13 and 1.30 mm in diameter, respectively, and the second is embedded in a large amount of mucus. The eggs grow, mainly due to water absorption at the beginning of the process, and they reach a diameter of about 1.35 and 1.59 mm, respectively, close to hatching. It took, respectively, 29–56 days and 35–66 days from egg laying to hatching. For the description of the embryonic development, we use photographs from the field, SEM micrographs, and histological analysis. This allowed us, for instance, to document the progression of structures and pigmentation directly from live embryos in the field, and to record microstructures, such as the presence of perforations in the cuticle of the embryo in the place where eyes are developing. Yet, contrary to what was expected in the literature, we record an egg tooth in one of the studied laniatoreans. J. Exp. Zool. (Mol. Dev. Evol.) 314B:489–502, 2010. © 2010 Wiley-Liss, Inc.
Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine... more Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine-disruption testing. To establish a nondestructive swab sampling method, VTG induction in the epidermis of Cypriniformes and Perciformes species was investigated. Both VTG and estrogen receptor genes are expressed in epidermal cells. Immunoaffinity and mass fingerprint analyses show induction of identical VTG peptides in liver and epidermis. Induction of VTG by estradiol (E2) and bisphenol A (BPA) in the epidermis was quantified with homolog enzyme-linked immunosorbent assays. Initial values in juveniles and males were below 1 ng VTG/mL extraction buffer. Exposure to E2 led to values between 200 ng/mL and 4600 ng/mL in cyprinids and between 10 ng/mL and 81 ng/mL in perciforms. Exposure to BPA increased VTG amounts to 250 ng/mL in fathead minnows, 1360 ng/mL in goldfish, 100 ng/mL in zebrafish, and 12 ng/mL in bluegills. Serum VTG contents demonstrated a similar dose–response pattern in t...
This study focuses on some aspects of the natural history of the cave heteropteran Zelurus travas... more This study focuses on some aspects of the natural history of the cave heteropteran Zelurus travassosi Costa-Lima, 1940, which is found in several caves from the Ribeira Valley – state of Sao Paulo. The study includes (1) the population dynamics, using mark-recapture methodology and estimation of the population number in Parque Estadual Intervales; (2) the life cycle, by means of rearing the different stages in the laboratory; and (3) morphometrics of each stage. In addition, observations about predatory behavior, reproductive behavior and spatial distribution within the caves have been made. Grant FAPESP # 00/03384-4
Environmental toxicology and chemistry / SETAC, Dec 6, 2016
Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine... more Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine disruption testing. In order to establish a nondestructive swab sampling method, VTG induction in the epidermis of Cypriniformes and Perciformes species has been investigated. Both vitellogenin and estrogen receptor genes are expressed in epidermal cells. Immunoaffinity and mass fingerprint analyses show induction of identical VTG peptides in liver and epidermis. Vitellogenin induction by estradiol (E2) and bisphenol A (BPA) in the epidermis was quantified with homolog enzyme-linked immunosorbent assays (ELISA). Initial values in juveniles and males were below 1 ng VTG/mL extraction buffer. Exposure to E2 led to values between 200 ng/mL and 4600 ng/mL in cyprinids and between 10 ng/mL and 81 ng/mL in perciforms. Exposure to BPA increased VTG amounts to 250 ng/mL in fathead minnows, 1360 ng/mL in goldfish, 100 ng/mL in zebrafish and 12 ng/mL in bluegills. VTG contents in the serum demons...
Journal of Experimental Zoology Part B-molecular and Developmental Evolution, 2010
The first studies concerning the embryonic development of harvestmen started in the late 19th cen... more The first studies concerning the embryonic development of harvestmen started in the late 19th century, and focused mostly on holarctic species, and only three species of the suborder Laniatores (the largest, among the four suborders considered presently) were studied. Moreover, the last studies on embryology of harvestmen were made during the late 1970s. This study focused on the embryonic development of Ampheres leucopheus (Gonyleptidae, Caelopyginae) and Iporangaia pustulosa (Gonyleptidae, Progonyleptoidellinae). The embryonic development was followed in the field, by taking daily photographs of different eggs during about 2 months. When laid, eggs of A. leucopheus and I. pustulosa have approximately 1.13 and 1.30 mm in diameter, respectively, and the second is embedded in a large amount of mucus. The eggs grow, mainly due to water absorption at the beginning of the process, and they reach a diameter of about 1.35 and 1.59 mm, respectively, close to hatching. It took, respectively, 29–56 days and 35–66 days from egg laying to hatching. For the description of the embryonic development, we use photographs from the field, SEM micrographs, and histological analysis. This allowed us, for instance, to document the progression of structures and pigmentation directly from live embryos in the field, and to record microstructures, such as the presence of perforations in the cuticle of the embryo in the place where eyes are developing. Yet, contrary to what was expected in the literature, we record an egg tooth in one of the studied laniatoreans. J. Exp. Zool. (Mol. Dev. Evol.) 314B:489–502, 2010. © 2010 Wiley-Liss, Inc.