Cristina Junta - Academia.edu (original) (raw)
Papers by Cristina Junta
Reproductive BioMedicine Online, 2011
Claudia G Petersen graduated in 1985 in biology from Federal University of São Carlos (UFSCAR), B... more Claudia G Petersen graduated in 1985 in biology from Federal University of São Carlos (UFSCAR), Brazil. She works at the Human Reproduction Center Prof Franco Jr at Ribeirão Preto, Brazil since 1990, coordinating the IVF laboratory team. In 2003, she obtained her Masters degree in clinical embryology at the School of Medicine at the University of Leeds, UK and in 2009 her PhD in science at the Botucatu Medical School, Brazil. Her current research interests have focused on gamete and embryo morphology, implantation and cryobiology.
Human Reproduction, 2010
Results: Native nondecondensed fluorochrome-labeled sperms showed a significant under-staining re... more Results: Native nondecondensed fluorochrome-labeled sperms showed a significant under-staining resulting in FI emission corresponding to a C-value variable around 1, 4 pg for haploid cells. Distributions of FI after our sperm head decondensation protocol proved an increase of MFC of the sample in accordance to the correct stoichiometric staining of sperm DNA (haploid human C-value 3.56 pg), making the diploid over aploid sperms frequency (7.12 pg) quantification very easy. Both DTT and Heparin at specific concentration were ...
Human Reproduction, Jun 1, 2010
Sao Paulo State Univ UNESP, Botucatu Med Sch, Ctr Human Reprod Prof Franco Jr,Res Unit, Paulista ... more Sao Paulo State Univ UNESP, Botucatu Med Sch, Ctr Human Reprod Prof Franco Jr,Res Unit, Paulista Ctr Diag Res & Training,Dept Gynecol & O, Ribeirao Preto, Brazil
Inflammation Research, Jan 17, 2012
Fungal Biology, Sep 1, 2021
Human Reproduction, Jun 1, 2010
Sao Paulo State Univ UNESP, Ctr Human Reprod Prof Franco Jr, Paulista Ctr Diag Res & Training... more Sao Paulo State Univ UNESP, Ctr Human Reprod Prof Franco Jr, Paulista Ctr Diag Res & Training, Dept Gynecol & Obstet,Botucatu Med Sch,Res Unit, Ribeirao Preto, Brazil
Acta Scientiae Veterinariae, 2007
Fungal biology, 2020
In eukaryotes, phosphorylation of the α-subunit of eIF2 is a mechanism to adjust cellular gene ex... more In eukaryotes, phosphorylation of the α-subunit of eIF2 is a mechanism to adjust cellular gene expression profiles in response to specific signals. The eIF2α kinases are a group of serine-threonine kinases that perform important functions in response to infection, proteotoxicity, and nutrient scavenging. The conserved nature of eIF2α kinases among fungi makes them potential evolutionary markers, which may contribute to deeper understanding of taxonomy and evolution. To date, only few studies are available of eIF2α kinases in black yeasts, which are members of Chaetothyriales containing potential agents of a gamut of major human diseases, such as chromoblastomycosis, phaeohyphomycosis and mycetoma. To establish the phylogenetic validity of sequences of eIF2α kinases hypothetical genes, we compared these genes between members of different classes of fungi, including black yeasts and allies, aiming at evaluation of the phylogeny of this group using an alternative molecular marker, comp...
Endocrinology&Metabolism International Journal, 2018
The central nervous system (CNS) plays an important role in food intake and energy expenditure re... more The central nervous system (CNS) plays an important role in food intake and energy expenditure regulation.1 In the CNS, the ARC is suggested to be a key hub for energy balance, reducing food intake and improving energy expenditure through leptin and insulin interactions with their receptors, ObR and IR, respectively (Coll et al., 2007). An important step for physiological ligand-mediated cell signaling is the maintenance of a steady state of functional receptors at the plasma membrane, which involves dynamic intracellular transport mechanisms.2 Leptin and insulin receptors require internalization and retrograde routes upon the binding of their ligands,3 then sorted and transported by vesicles, for direct recycling to the plasma membrane or via the Trans-Golgi Network (TGN) or lysosomal degradation.4 Retromer, a protein trimer, is essential for the recruitment of nucleation-promoting factors to endosomes, including the WASH complex, which has a carboxy-terminal VCA motif that binds to actin, allowing subsequent actin filament nucleation by the Arp2/3 complex.5,6 On the other hand, non-recycled cargoes are directed to lysosomal degradation pathways. Newly synthesized hydrolases acquire a mannose-6-phosphate marker during their maturation and are then transported by the mannose-6-phosphate receptors (CDM6PR, CIM6PR also named IGFR2) in a step for lysosomal formation.7,8 In this study, we evaluated the transcript levels of genes involved in endosomal recycling in a murine model of HFD induced obesity and showed that HFD fed mice presented altered transcript levels for Ube2o, Igfr2, Cdm6pr and Tbc1d5. And showed significant body weight gain and hyperglycemia.
Genetics and Molecular Biology, 1998
Clinical and experimental rheumatology
To evaluate the gene expression profile of fibroblasts from affected and non-affected skin of sys... more To evaluate the gene expression profile of fibroblasts from affected and non-affected skin of systemic sclerosis (SSc) patients and from controls. Labeled cDNA from fibroblast cultures from forearm (affected) and axillary (non-affected) skin from six diffuse SSc patients, from three normal controls, and from MOLT-4/HEp-2/normal fibroblasts (reference pool) was probed in microarrays generated with 4193 human cDNAs from the IMAGE Consortium. Microarray images were converted into numerical data and gene expression was calculated as the ratio between fibroblast cDNA (Cy5) and reference pool cDNA (Cy3) data and analyzed by R environment/Aroma, Cluster, Tree View, and SAM softwares. Differential expression was confirmed by real time PCR for a set of selected genes. Eighty-eight genes were up- and 241 genes down-regulated in SSc fibroblasts. Gene expression correlation was strong between affected and non-affected fibroblast samples from the same patient (r>0.8), moderate among fibroblas...
Molecular and cellular biochemistry, 1998
The maturation of T-cells depends on V(D)J recombination at the TCR alpha/beta and gamma/delta lo... more The maturation of T-cells depends on V(D)J recombination at the TCR alpha/beta and gamma/delta loci that occurs in the thymus during fetal development. Due to the essential role of the TCRbeta in thymocyte differentiation, it is important to define with precision the temporal emergence of the TCRbeta rearrangement and its expression in normal non-manipulated mouse strains. We studied the onset of the V(D)J recombination and transcription of the TCR Valpha3 and Vbeta11 genes during ontogeny in Balb-c, C57B1/6 and CBA inbred mouse strains. Our data show differences in the emergence of recombination in both TCR alpha and beta loci among strains. The transcriptions of these loci followed respective recombinations and we detected an early germline transcript before TCR beta locus recombination in the CBA strain.
Scandinavian Journal of Immunology, 2004
To evaluate the distribution of usage and to quantify the transcription levels of the immunoglobu... more To evaluate the distribution of usage and to quantify the transcription levels of the immunoglobulin lambda variable (IGLV) genes in patients with systemic lupus erythematosus (SLE) and normal individuals (NIs), cDNA samples from peripheral blood lymphocytes were prepared and probed with IGLV-specific oligonucleotides. Because recombinations involving V-lambda pseudogenes are nonproductive, we analysed the IGLV productive repertoire, as cDNAs were copied from IGLV mRNA producing B lymphocytes. Increased expression of the IGLV8a gene in SLE led us to analyse the transcription levels of all IGLV genes. We developed an expression profiling approach to scan the entire V-lambda locus on chromosome 22q11.2. The transcription profiling showed that usually the V-lambda genes located near the Jlambda-Clambda cluster were preferentially expressed in both groups, i.e. patients and NIs, with the expression levels of SLE patients being significantly higher. However, genes displaying peaks of expression independent of Jlambda-Clambda cluster proximity were observed along the IGLV locus. Our data permit us to conclude that there are differences in V-lambda gene expression between SLE patients and NIs, and a preferential usage of genes located near the Jlambda-Clambda cluster. The data also demonstrate the occurrence of Vlambda-Jlambda-Clambda-productive recombinations independent of gene localization along the locus.
Mutation Research/Reviews in Mutation Research, 2003
Cell response to genotoxic agents is complex and involves the participation of different classes ... more Cell response to genotoxic agents is complex and involves the participation of different classes of genes (DNA repair, cell cycle control, signal transduction, apoptosis and oncogenesis). In this report, we present three approaches to document gene expression profiles, dealing with the evaluation of cellular responses to genotoxic agents (gamma-rays from 60Cobalt and cyclophosphamide). We used the method of cDNA arrays to analyze the differential gene expression profiles that were displayed by lymphocytes from radiation-exposed individuals, a human fibroblast cell line, and T lymphocytes from systemic lupus erythematosus (SLE) patients who were treated with cyclophosphamide. A preliminary analysis performed in lymphocytes from three radiation-workers showed that several induced genes can be associated with cell response to ionizing radiation: TRRAP (cell cycle regulation), Ligase IV (DNA repair), MAPK8IP1 and MAPK10 (signal transduction), RASSF2 (apoptosis induction/tumorigenesis), p53 (damage response/maintenance of genetic stability). The in vitro irradiated normal VH16 cell line (primary) showed a complex response to the genotoxic stress at the molecular level. Many apoptotic pathways were concomitantly induced. In addition, several genes involved in signaling and cell cycle arrest/control were significantly modulated after irradiation. Many genes involved in oxidative damage were also induced, indicating that this mechanism seems to be an important component of cell response. After treatment of the SLE patients with cyclophosphamide, 154 genes were differentially and significantly induced. Among them, we identified those associated with drug detoxification, cell cycle control, apoptosis, and tumor-suppressor. These findings indicate that at least two apoptotic pathways were induced after cyclophosphamide treatment. The induction of APAF1 and two genes coding for two subunits of cytochrome c supports a previous report showing increased apoptosis in lymphocytes from SLE patients. The present study provides new information on the molecular mechanism underlying the cell response to genotoxic stress, with relevance to basic and clinical research.
Reproductive BioMedicine Online, 2011
Claudia G Petersen graduated in 1985 in biology from Federal University of São Carlos (UFSCAR), B... more Claudia G Petersen graduated in 1985 in biology from Federal University of São Carlos (UFSCAR), Brazil. She works at the Human Reproduction Center Prof Franco Jr at Ribeirão Preto, Brazil since 1990, coordinating the IVF laboratory team. In 2003, she obtained her Masters degree in clinical embryology at the School of Medicine at the University of Leeds, UK and in 2009 her PhD in science at the Botucatu Medical School, Brazil. Her current research interests have focused on gamete and embryo morphology, implantation and cryobiology.
Human Reproduction, 2010
Results: Native nondecondensed fluorochrome-labeled sperms showed a significant under-staining re... more Results: Native nondecondensed fluorochrome-labeled sperms showed a significant under-staining resulting in FI emission corresponding to a C-value variable around 1, 4 pg for haploid cells. Distributions of FI after our sperm head decondensation protocol proved an increase of MFC of the sample in accordance to the correct stoichiometric staining of sperm DNA (haploid human C-value 3.56 pg), making the diploid over aploid sperms frequency (7.12 pg) quantification very easy. Both DTT and Heparin at specific concentration were ...
Human Reproduction, Jun 1, 2010
Sao Paulo State Univ UNESP, Botucatu Med Sch, Ctr Human Reprod Prof Franco Jr,Res Unit, Paulista ... more Sao Paulo State Univ UNESP, Botucatu Med Sch, Ctr Human Reprod Prof Franco Jr,Res Unit, Paulista Ctr Diag Res & Training,Dept Gynecol & O, Ribeirao Preto, Brazil
Inflammation Research, Jan 17, 2012
Fungal Biology, Sep 1, 2021
Human Reproduction, Jun 1, 2010
Sao Paulo State Univ UNESP, Ctr Human Reprod Prof Franco Jr, Paulista Ctr Diag Res & Training... more Sao Paulo State Univ UNESP, Ctr Human Reprod Prof Franco Jr, Paulista Ctr Diag Res & Training, Dept Gynecol & Obstet,Botucatu Med Sch,Res Unit, Ribeirao Preto, Brazil
Acta Scientiae Veterinariae, 2007
Fungal biology, 2020
In eukaryotes, phosphorylation of the α-subunit of eIF2 is a mechanism to adjust cellular gene ex... more In eukaryotes, phosphorylation of the α-subunit of eIF2 is a mechanism to adjust cellular gene expression profiles in response to specific signals. The eIF2α kinases are a group of serine-threonine kinases that perform important functions in response to infection, proteotoxicity, and nutrient scavenging. The conserved nature of eIF2α kinases among fungi makes them potential evolutionary markers, which may contribute to deeper understanding of taxonomy and evolution. To date, only few studies are available of eIF2α kinases in black yeasts, which are members of Chaetothyriales containing potential agents of a gamut of major human diseases, such as chromoblastomycosis, phaeohyphomycosis and mycetoma. To establish the phylogenetic validity of sequences of eIF2α kinases hypothetical genes, we compared these genes between members of different classes of fungi, including black yeasts and allies, aiming at evaluation of the phylogeny of this group using an alternative molecular marker, comp...
Endocrinology&Metabolism International Journal, 2018
The central nervous system (CNS) plays an important role in food intake and energy expenditure re... more The central nervous system (CNS) plays an important role in food intake and energy expenditure regulation.1 In the CNS, the ARC is suggested to be a key hub for energy balance, reducing food intake and improving energy expenditure through leptin and insulin interactions with their receptors, ObR and IR, respectively (Coll et al., 2007). An important step for physiological ligand-mediated cell signaling is the maintenance of a steady state of functional receptors at the plasma membrane, which involves dynamic intracellular transport mechanisms.2 Leptin and insulin receptors require internalization and retrograde routes upon the binding of their ligands,3 then sorted and transported by vesicles, for direct recycling to the plasma membrane or via the Trans-Golgi Network (TGN) or lysosomal degradation.4 Retromer, a protein trimer, is essential for the recruitment of nucleation-promoting factors to endosomes, including the WASH complex, which has a carboxy-terminal VCA motif that binds to actin, allowing subsequent actin filament nucleation by the Arp2/3 complex.5,6 On the other hand, non-recycled cargoes are directed to lysosomal degradation pathways. Newly synthesized hydrolases acquire a mannose-6-phosphate marker during their maturation and are then transported by the mannose-6-phosphate receptors (CDM6PR, CIM6PR also named IGFR2) in a step for lysosomal formation.7,8 In this study, we evaluated the transcript levels of genes involved in endosomal recycling in a murine model of HFD induced obesity and showed that HFD fed mice presented altered transcript levels for Ube2o, Igfr2, Cdm6pr and Tbc1d5. And showed significant body weight gain and hyperglycemia.
Genetics and Molecular Biology, 1998
Clinical and experimental rheumatology
To evaluate the gene expression profile of fibroblasts from affected and non-affected skin of sys... more To evaluate the gene expression profile of fibroblasts from affected and non-affected skin of systemic sclerosis (SSc) patients and from controls. Labeled cDNA from fibroblast cultures from forearm (affected) and axillary (non-affected) skin from six diffuse SSc patients, from three normal controls, and from MOLT-4/HEp-2/normal fibroblasts (reference pool) was probed in microarrays generated with 4193 human cDNAs from the IMAGE Consortium. Microarray images were converted into numerical data and gene expression was calculated as the ratio between fibroblast cDNA (Cy5) and reference pool cDNA (Cy3) data and analyzed by R environment/Aroma, Cluster, Tree View, and SAM softwares. Differential expression was confirmed by real time PCR for a set of selected genes. Eighty-eight genes were up- and 241 genes down-regulated in SSc fibroblasts. Gene expression correlation was strong between affected and non-affected fibroblast samples from the same patient (r>0.8), moderate among fibroblas...
Molecular and cellular biochemistry, 1998
The maturation of T-cells depends on V(D)J recombination at the TCR alpha/beta and gamma/delta lo... more The maturation of T-cells depends on V(D)J recombination at the TCR alpha/beta and gamma/delta loci that occurs in the thymus during fetal development. Due to the essential role of the TCRbeta in thymocyte differentiation, it is important to define with precision the temporal emergence of the TCRbeta rearrangement and its expression in normal non-manipulated mouse strains. We studied the onset of the V(D)J recombination and transcription of the TCR Valpha3 and Vbeta11 genes during ontogeny in Balb-c, C57B1/6 and CBA inbred mouse strains. Our data show differences in the emergence of recombination in both TCR alpha and beta loci among strains. The transcriptions of these loci followed respective recombinations and we detected an early germline transcript before TCR beta locus recombination in the CBA strain.
Scandinavian Journal of Immunology, 2004
To evaluate the distribution of usage and to quantify the transcription levels of the immunoglobu... more To evaluate the distribution of usage and to quantify the transcription levels of the immunoglobulin lambda variable (IGLV) genes in patients with systemic lupus erythematosus (SLE) and normal individuals (NIs), cDNA samples from peripheral blood lymphocytes were prepared and probed with IGLV-specific oligonucleotides. Because recombinations involving V-lambda pseudogenes are nonproductive, we analysed the IGLV productive repertoire, as cDNAs were copied from IGLV mRNA producing B lymphocytes. Increased expression of the IGLV8a gene in SLE led us to analyse the transcription levels of all IGLV genes. We developed an expression profiling approach to scan the entire V-lambda locus on chromosome 22q11.2. The transcription profiling showed that usually the V-lambda genes located near the Jlambda-Clambda cluster were preferentially expressed in both groups, i.e. patients and NIs, with the expression levels of SLE patients being significantly higher. However, genes displaying peaks of expression independent of Jlambda-Clambda cluster proximity were observed along the IGLV locus. Our data permit us to conclude that there are differences in V-lambda gene expression between SLE patients and NIs, and a preferential usage of genes located near the Jlambda-Clambda cluster. The data also demonstrate the occurrence of Vlambda-Jlambda-Clambda-productive recombinations independent of gene localization along the locus.
Mutation Research/Reviews in Mutation Research, 2003
Cell response to genotoxic agents is complex and involves the participation of different classes ... more Cell response to genotoxic agents is complex and involves the participation of different classes of genes (DNA repair, cell cycle control, signal transduction, apoptosis and oncogenesis). In this report, we present three approaches to document gene expression profiles, dealing with the evaluation of cellular responses to genotoxic agents (gamma-rays from 60Cobalt and cyclophosphamide). We used the method of cDNA arrays to analyze the differential gene expression profiles that were displayed by lymphocytes from radiation-exposed individuals, a human fibroblast cell line, and T lymphocytes from systemic lupus erythematosus (SLE) patients who were treated with cyclophosphamide. A preliminary analysis performed in lymphocytes from three radiation-workers showed that several induced genes can be associated with cell response to ionizing radiation: TRRAP (cell cycle regulation), Ligase IV (DNA repair), MAPK8IP1 and MAPK10 (signal transduction), RASSF2 (apoptosis induction/tumorigenesis), p53 (damage response/maintenance of genetic stability). The in vitro irradiated normal VH16 cell line (primary) showed a complex response to the genotoxic stress at the molecular level. Many apoptotic pathways were concomitantly induced. In addition, several genes involved in signaling and cell cycle arrest/control were significantly modulated after irradiation. Many genes involved in oxidative damage were also induced, indicating that this mechanism seems to be an important component of cell response. After treatment of the SLE patients with cyclophosphamide, 154 genes were differentially and significantly induced. Among them, we identified those associated with drug detoxification, cell cycle control, apoptosis, and tumor-suppressor. These findings indicate that at least two apoptotic pathways were induced after cyclophosphamide treatment. The induction of APAF1 and two genes coding for two subunits of cytochrome c supports a previous report showing increased apoptosis in lymphocytes from SLE patients. The present study provides new information on the molecular mechanism underlying the cell response to genotoxic stress, with relevance to basic and clinical research.