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Papers by DANIEL STIVEN CAMACHO GUZMAN

Research paper thumbnail of A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

Biomedicines, 2020

Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increa... more Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increasingly reliable and accessible. Particularly, the identification of circulating cell-free chemical and biochemical substances, cellular and subcellular entities, and extracellular vesicles has demonstrated promising applications in understanding the physiologic and pathologic conditions of an individual. Traditionally, tissue biopsy has been the gold standard for the diagnosis of many diseases, especially cancer. More recently, liquid biopsy for biomarker detection has emerged as a non-invasive or minimally invasive and less costly method for diagnosis of both cancerous and non-cancerous diseases, while also offering information on the progression or improvement of disease. Unfortunately, the standardization of analytical methods to isolate and quantify circulating cells and extracellular vesicles, as well as their extracted biochemical constituents, is still cumbersome, time-consuming, ...

Research paper thumbnail of Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

Biomolecules, 2021

Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, progno... more Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, prognosis, and response to therapy. As of late, protein biomarkers are gaining strength in their role for early disease diagnosis and prognosis in part due to the advancements in identification and characterization of a distinct functional pool of proteins known as proteoforms. Proteoforms are defined as all of the different molecular forms of a protein derived from a single gene caused by genetic variations, alternative spliced RNA transcripts and post-translational modifications. Monitoring the structural changes of each proteoform of a particular protein is essential to elucidate the complex molecular mechanisms that guide the course of disease. Clinical proteomics therefore holds the potential to offer further insight into disease pathology, progression, and prevention. Nevertheless, more technologically advanced diagnostic methods are needed to improve the reliability and clinical applica...

Research paper thumbnail of Diversity and Inclusion in the Academic Medicine Workforce: Encouraging Medical Students and Residents to Consider Academic Careers

MedEdPORTAL, 2018

Introduction: The expansion of medical schools and increased faculty attrition call for heightene... more Introduction: The expansion of medical schools and increased faculty attrition call for heightened efforts to encourage medical students and residents to consider academic careers. As diversity serves as a driver of institutional excellence, special attention to the ongoing underrepresentation of certain groups in academia, such as racial and ethnic minorities, women, and lesbian, gay, bisexual, and transgender individuals, is warranted. Methods: We developed a 90-minute workshop to raise medical student and resident awareness of academic medicine careers, and the benefits and challenges of having a diverse faculty. The workshop consists of a didactic PowerPoint presentation and a reflection exercise, shared in small-and large-group format, discussing facilitators and barriers to pursuing academia. The workshop was implemented at nine regional conferences. Results: There were 165 diverse participants. In comparing pre-and postworkshop responses of learners using the sample t test, there was a statistically significant increase in confidence to succeed in academic medicine given learners' gender (2.69 vs. 3.34, p < .001), race and ethnicity (2.53 vs. 3.24, p < .001), or sexual orientation (3.04 vs. 3.42, p < .001). Approximately 95% of learners felt that each of the workshop's learning objectives had been achieved. Participants considered the workshop to be enlightening, motivational, realistic, and validating. Discussion: This workshop was effective in providing an interactive format for medical students and residents to gain awareness of the state, benefits, and challenges of diversity and inclusion in academic medicine, and can affect their perception of being a future faculty member.

Research paper thumbnail of EFECTO DEL VENENO DE Hadruroides charcasus SOBRE EL TEJIDO CARDIACO DE Bufo spinulosus

Pharmaciencia, Sep 9, 2014

EFECTO DEL VENENO DE Hadruroides charcasus SOBRE EL TEJIDO CARDIACO DE Bufo spinulosus Effect of ... more EFECTO DEL VENENO DE Hadruroides charcasus SOBRE EL TEJIDO CARDIACO DE Bufo spinulosus Effect of Hadruroides charcasus venom on cardiac tissue of Bufo spinulosus

Research paper thumbnail of An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for precision medicine and P4 medicine

Journal of Chromatography B, 2016

An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for ... more An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for precision medicine and P4 medicine,

Research paper thumbnail of Sorting out problems manifested by peritoneal irritation

Clinical Pediatric Emergency Medicine, 2002

Research paper thumbnail of Capnometry for noninvasive continuous monitoring of metabolic status in pediatric diabetic ketoacidosis*

Critical Care Medicine, 2003

To determine the utility of continuous noninvasive capnometry for monitoring pediatric patients w... more To determine the utility of continuous noninvasive capnometry for monitoring pediatric patients with diabetic ketoacidosis as assessed by the agreement between end-tidal carbon dioxide (PetCO2) and PCO2 Clinical, prospective, observational study. University affiliated children&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s hospital. Patients with diabetic ketoacidosis were monitored with an oral/nasal carbon dioxide (CO2) sampling cannula while in the emergency department. Laboratory studies were ordered per protocol. PetCO2 values were correlated with respiratory rate, PCO2, and pH. One hundred twenty-one patients were monitored for 5.9 +/- 0.32 hrs. The average (mean +/- sd) initial values for pH were 7.08 +/- 0.18; respiratory rate, 35.1 +/- 9.1 breaths/min; PetCO2, 18.6 +/- 10.8 torr; and venous PCO2, 20.2 +/- 10.6 torr. At the conclusion of the observation period, averages were pH, 7.29 +/- 0.05; respiratory rate, 22.4 +/- 3.7 breaths/min; PetCO2, 35.3 +/- 5.8 torr; and venous PCO2, 36.8 +/- 5.3 torr. For all 592 observations, the correlations between PetCO2 and venous PCO2 (r =.92, p =.0001), PetCO2 and pH (r =.88, p =.0001), Petco2 and respiratory rate (r = -.79, p =.0001), and respiratory rate and pH (r = -.80, p =.0001) were statistically significant and the correlations with respiratory rate were inversely related to pH and PetCO2. The difference scores were not related to the average scores for initial readings (r = -.073, p =.43), final readings (r = -.124, p =.18), and overall readings (r =.057, p =.17). Limits of agreement between the two methods were established with PetCO2 lower than venous PCO2 with 95% limits of agreement 0.8 +/- 8.3 (2 sd) torr. PetCO2 monitoring of patients with diabetic ketoacidosis provides an accurate estimate of PCO2. Noninvasive PetCO2 sampling may be useful in patients with diabetic ketoacidosis to allow for continuous monitoring of patients.

Research paper thumbnail of A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

Biomedicines, 2020

Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increa... more Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increasingly reliable and accessible. Particularly, the identification of circulating cell-free chemical and biochemical substances, cellular and subcellular entities, and extracellular vesicles has demonstrated promising applications in understanding the physiologic and pathologic conditions of an individual. Traditionally, tissue biopsy has been the gold standard for the diagnosis of many diseases, especially cancer. More recently, liquid biopsy for biomarker detection has emerged as a non-invasive or minimally invasive and less costly method for diagnosis of both cancerous and non-cancerous diseases, while also offering information on the progression or improvement of disease. Unfortunately, the standardization of analytical methods to isolate and quantify circulating cells and extracellular vesicles, as well as their extracted biochemical constituents, is still cumbersome, time-consuming, ...

Research paper thumbnail of Immunoaffinity Capillary Electrophoresis in the Era of Proteoforms, Liquid Biopsy and Preventive Medicine: A Potential Impact in the Diagnosis and Monitoring of Disease Progression

Biomolecules, 2021

Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, progno... more Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, prognosis, and response to therapy. As of late, protein biomarkers are gaining strength in their role for early disease diagnosis and prognosis in part due to the advancements in identification and characterization of a distinct functional pool of proteins known as proteoforms. Proteoforms are defined as all of the different molecular forms of a protein derived from a single gene caused by genetic variations, alternative spliced RNA transcripts and post-translational modifications. Monitoring the structural changes of each proteoform of a particular protein is essential to elucidate the complex molecular mechanisms that guide the course of disease. Clinical proteomics therefore holds the potential to offer further insight into disease pathology, progression, and prevention. Nevertheless, more technologically advanced diagnostic methods are needed to improve the reliability and clinical applica...

Research paper thumbnail of Diversity and Inclusion in the Academic Medicine Workforce: Encouraging Medical Students and Residents to Consider Academic Careers

MedEdPORTAL, 2018

Introduction: The expansion of medical schools and increased faculty attrition call for heightene... more Introduction: The expansion of medical schools and increased faculty attrition call for heightened efforts to encourage medical students and residents to consider academic careers. As diversity serves as a driver of institutional excellence, special attention to the ongoing underrepresentation of certain groups in academia, such as racial and ethnic minorities, women, and lesbian, gay, bisexual, and transgender individuals, is warranted. Methods: We developed a 90-minute workshop to raise medical student and resident awareness of academic medicine careers, and the benefits and challenges of having a diverse faculty. The workshop consists of a didactic PowerPoint presentation and a reflection exercise, shared in small-and large-group format, discussing facilitators and barriers to pursuing academia. The workshop was implemented at nine regional conferences. Results: There were 165 diverse participants. In comparing pre-and postworkshop responses of learners using the sample t test, there was a statistically significant increase in confidence to succeed in academic medicine given learners' gender (2.69 vs. 3.34, p < .001), race and ethnicity (2.53 vs. 3.24, p < .001), or sexual orientation (3.04 vs. 3.42, p < .001). Approximately 95% of learners felt that each of the workshop's learning objectives had been achieved. Participants considered the workshop to be enlightening, motivational, realistic, and validating. Discussion: This workshop was effective in providing an interactive format for medical students and residents to gain awareness of the state, benefits, and challenges of diversity and inclusion in academic medicine, and can affect their perception of being a future faculty member.

Research paper thumbnail of EFECTO DEL VENENO DE Hadruroides charcasus SOBRE EL TEJIDO CARDIACO DE Bufo spinulosus

Pharmaciencia, Sep 9, 2014

EFECTO DEL VENENO DE Hadruroides charcasus SOBRE EL TEJIDO CARDIACO DE Bufo spinulosus Effect of ... more EFECTO DEL VENENO DE Hadruroides charcasus SOBRE EL TEJIDO CARDIACO DE Bufo spinulosus Effect of Hadruroides charcasus venom on cardiac tissue of Bufo spinulosus

Research paper thumbnail of An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for precision medicine and P4 medicine

Journal of Chromatography B, 2016

An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for ... more An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for precision medicine and P4 medicine,

Research paper thumbnail of Sorting out problems manifested by peritoneal irritation

Clinical Pediatric Emergency Medicine, 2002

Research paper thumbnail of Capnometry for noninvasive continuous monitoring of metabolic status in pediatric diabetic ketoacidosis*

Critical Care Medicine, 2003

To determine the utility of continuous noninvasive capnometry for monitoring pediatric patients w... more To determine the utility of continuous noninvasive capnometry for monitoring pediatric patients with diabetic ketoacidosis as assessed by the agreement between end-tidal carbon dioxide (PetCO2) and PCO2 Clinical, prospective, observational study. University affiliated children&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s hospital. Patients with diabetic ketoacidosis were monitored with an oral/nasal carbon dioxide (CO2) sampling cannula while in the emergency department. Laboratory studies were ordered per protocol. PetCO2 values were correlated with respiratory rate, PCO2, and pH. One hundred twenty-one patients were monitored for 5.9 +/- 0.32 hrs. The average (mean +/- sd) initial values for pH were 7.08 +/- 0.18; respiratory rate, 35.1 +/- 9.1 breaths/min; PetCO2, 18.6 +/- 10.8 torr; and venous PCO2, 20.2 +/- 10.6 torr. At the conclusion of the observation period, averages were pH, 7.29 +/- 0.05; respiratory rate, 22.4 +/- 3.7 breaths/min; PetCO2, 35.3 +/- 5.8 torr; and venous PCO2, 36.8 +/- 5.3 torr. For all 592 observations, the correlations between PetCO2 and venous PCO2 (r =.92, p =.0001), PetCO2 and pH (r =.88, p =.0001), Petco2 and respiratory rate (r = -.79, p =.0001), and respiratory rate and pH (r = -.80, p =.0001) were statistically significant and the correlations with respiratory rate were inversely related to pH and PetCO2. The difference scores were not related to the average scores for initial readings (r = -.073, p =.43), final readings (r = -.124, p =.18), and overall readings (r =.057, p =.17). Limits of agreement between the two methods were established with PetCO2 lower than venous PCO2 with 95% limits of agreement 0.8 +/- 8.3 (2 sd) torr. PetCO2 monitoring of patients with diabetic ketoacidosis provides an accurate estimate of PCO2. Noninvasive PetCO2 sampling may be useful in patients with diabetic ketoacidosis to allow for continuous monitoring of patients.