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Applied biochemistry and biotechnology, Jan 2, 2018
The present study reports beneficial effect of hydroxytyrosol (HT) against arsenic (As)-induced o... more The present study reports beneficial effect of hydroxytyrosol (HT) against arsenic (As)-induced oxidative stress in the rat brain. Rats were orally administered with sodium arsenite dissolved in distilled water (25 ppm, by oral gavage) for 8 weeks or HT (10 mg/kg b. wt.) in combination with As. Results showed increase in protein oxidation and lipid peroxidation, while catalase and superoxide dismutase (SOD) activities as well as GSH content were decreased after As exposure in rat brain. Fourier transform infrared analysis showed significant alteration in peak area values that also validated the oxidative damage to lipids and proteins. In addition, As exposure caused increase in protein expression of caspase-3 and Bax, while Bcl-2 expression was downregulated resulting in translocation of cytochrome c from mitochondria to cytosol. Treatment of HT with As reversed protein oxidation, lipid peroxidation, and increased GSH content as well as catalase and SOD activities. Administration of...
Bottarga, the salted and semidried mullet (Mugil cephalus) ovary product, is proposed as importan... more Bottarga, the salted and semidried mullet (Mugil cephalus) ovary product, is proposed as important source of n-3 PUFA, having high levels of biologically active eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In this work the modulatory effects on cell viability, lipid profile, and oxidative status in normal and cancer intestinal cells after incubation with lipid and hydrophilic extracts obtained from grated bottarga samples at different condition of aging/ browning was investigated. Bottarga lipid extracts did not show a toxic effect on cell viability in normal intestinal epithelial cells, exhibiting a significant viability reduction in colonic cancer cells. Bottarga oil showed the ability to induce significant changes on lipid composition in normal and cancer intestinal cells, with a significant accumulation of EPA, DHA, and 22:5. The obtained results qualify bottarga as an important natural, biodisponible, stable, source of n-3 PUFA, potentially able to induce benefic...
Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinia... more Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinian bottarga is the final product of a number of treatments (basically salting and drying) conducted on the ovaries of mullet. In this work main lipid classes, fatty acids and fatty alcohols compositions, and oxidative status of commercial samples of grated bottarga produced in Sardinia were studied by using different analytical techniques (GC, HPLC and 13C NMR). Wax esters resulted the main lipid fraction (>50 mol%), whereas triacylglycerols and phospholipids, cholesterol and cholesteryl-esters represented a minor fraction, with concentrations of free fatty acids in the range 15-29 mol%. The most represented fatty alcohols were 14:0, 16:0, 16:1 n-7 e 18:0, among fatty acids the most represented were 16:0, 18:0, 16:1 n-7, 18:1 n-9, 20:5 n-3 (EPA, 10-13 mg/g of the edible portion) and 22:6 n-3 (DHA, 20-29 mg/g). The oxidation status was measured by detection of conjugated dienes fatty ac...
Applied biochemistry and biotechnology, Jan 2, 2018
The present study reports beneficial effect of hydroxytyrosol (HT) against arsenic (As)-induced o... more The present study reports beneficial effect of hydroxytyrosol (HT) against arsenic (As)-induced oxidative stress in the rat brain. Rats were orally administered with sodium arsenite dissolved in distilled water (25 ppm, by oral gavage) for 8 weeks or HT (10 mg/kg b. wt.) in combination with As. Results showed increase in protein oxidation and lipid peroxidation, while catalase and superoxide dismutase (SOD) activities as well as GSH content were decreased after As exposure in rat brain. Fourier transform infrared analysis showed significant alteration in peak area values that also validated the oxidative damage to lipids and proteins. In addition, As exposure caused increase in protein expression of caspase-3 and Bax, while Bcl-2 expression was downregulated resulting in translocation of cytochrome c from mitochondria to cytosol. Treatment of HT with As reversed protein oxidation, lipid peroxidation, and increased GSH content as well as catalase and SOD activities. Administration of...
Bottarga, the salted and semidried mullet (Mugil cephalus) ovary product, is proposed as importan... more Bottarga, the salted and semidried mullet (Mugil cephalus) ovary product, is proposed as important source of n-3 PUFA, having high levels of biologically active eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In this work the modulatory effects on cell viability, lipid profile, and oxidative status in normal and cancer intestinal cells after incubation with lipid and hydrophilic extracts obtained from grated bottarga samples at different condition of aging/ browning was investigated. Bottarga lipid extracts did not show a toxic effect on cell viability in normal intestinal epithelial cells, exhibiting a significant viability reduction in colonic cancer cells. Bottarga oil showed the ability to induce significant changes on lipid composition in normal and cancer intestinal cells, with a significant accumulation of EPA, DHA, and 22:5. The obtained results qualify bottarga as an important natural, biodisponible, stable, source of n-3 PUFA, potentially able to induce benefic...
Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinia... more Sardinia has a long tradition in making a high quality mullet bottarga (Mugil cephalus). Sardinian bottarga is the final product of a number of treatments (basically salting and drying) conducted on the ovaries of mullet. In this work main lipid classes, fatty acids and fatty alcohols compositions, and oxidative status of commercial samples of grated bottarga produced in Sardinia were studied by using different analytical techniques (GC, HPLC and 13C NMR). Wax esters resulted the main lipid fraction (>50 mol%), whereas triacylglycerols and phospholipids, cholesterol and cholesteryl-esters represented a minor fraction, with concentrations of free fatty acids in the range 15-29 mol%. The most represented fatty alcohols were 14:0, 16:0, 16:1 n-7 e 18:0, among fatty acids the most represented were 16:0, 18:0, 16:1 n-7, 18:1 n-9, 20:5 n-3 (EPA, 10-13 mg/g of the edible portion) and 22:6 n-3 (DHA, 20-29 mg/g). The oxidation status was measured by detection of conjugated dienes fatty ac...