D. Poletaeva - Academia.edu (original) (raw)
Papers by D. Poletaeva
Membranes
This paper shows the biological effects of cationic binuclear tetranitrosyl iron complex with pen... more This paper shows the biological effects of cationic binuclear tetranitrosyl iron complex with penicillamine ligands (TNIC–PA). Interaction with a model membrane was assessed using a fluorescent probes technique. Antioxidant activity was studied using a thiobarbituric acid reactive species assay (TBARS) and a chemiluminescence assay. The catalytic activity of monoamine oxidase (MAO) was determined by measuring liberation of ammonia. Antiglycation activity was determined fluometrically by thermal glycation of albumine by D-glucose. The higher values of Stern–Volmer constants (KSV) obtained for the pyrene located in hydrophobic regions (3.9 × 104 M−1) compared to KSV obtained for eosin Y located in the polar headgroup region (0.9 × 104 M−1) confirms that TNIC–PA molecules prefer to be located in the hydrophobic acyl chain region, close to the glycerol group of lipid molecules. TNIC–PA effectively inhibited the process of spontaneous lipid peroxidation, due to additive contributions fro...
Mendeleev Communications, 2022
Doklady. Physical chemistry, 2016
The potential of surface enhanced Raman spectroscopy (SERS) for the detection of water-soluble fu... more The potential of surface enhanced Raman spectroscopy (SERS) for the detection of water-soluble fullerene derivatives and their covalent conjugates with xanthene dyes was investigated in model biological liposome membranes and in the albumin protein structure. It was shown that in liposomes and in albumin, fullerene derivatives and their covalent conjugates with dyes show characteristic SERS spectra, which allows detection of water-soluble fullerene derivatives in phosphatidylcholine liposomes at the lipid/fullerene derivative ratio of 100 as well as fullerene–dye conjugates in liposomes and albumin.
Journal of Polymer Research, 2021
The complexes of metformin (MET) and the copolymer of N-vinylpyrrolidone with triethylene glycol ... more The complexes of metformin (MET) and the copolymer of N-vinylpyrrolidone with triethylene glycol dimethacrylate with absolute molecular weight ca. 26 kDa and hydrodynamic radius of macromolecules about 4 nm have been prepared and studied in water buffer solution. Their sizes in neutral phosphate buffer solution depended on MET (10–40 wt%) per the copolymer content, temperature and the copolymer concentrations. Quantum chemical simulation has shown the most energetically favorable and stable structure is one with the coordination of neighboring NH2 groups of MET via oxygen carbonyl of the VP fragment. The effective binding constant of MET with the copolymer estimated from the absorption spectroscopy was found to be ~ 1.8 × 103 M−1. The MET-copolymer compositions were analyzed by IR spectroscopy, thermogravimetry, differential scanning calorimetry, and X-ray diffraction. The copolymer complex with 20 wt% of MET affected blood glucose level in streptozotocin-induced diabetic mice like MET and decreased aldose reductase activity in contrast with the drug and MET-PVP complex.
Nitric Oxide, 2021
Nitric oxide (NO) mediates diverse physiological processes in living organisms. Small molecular N... more Nitric oxide (NO) mediates diverse physiological processes in living organisms. Small molecular NO donors usually lack stability and have a short half-life in human tissues, limiting the therapeutic application. The anionic tetranitrosyl iron complex with thiosulfate ligands (TNIC) is one of the most promising NO donors. This study shows that bovine serum albumin (BSA) can effectively stabilize the TNIC complex under aerobic (physiological) conditions, which contributes to its prolonged action as NO donor. Our results demonstrated that TNIC-BSA inhibits formation of TBARS - standard biomarker for the lipid peroxidation induced oxidative stress. Also, it was found that TNIC-BSA inhibits the catalytic activity of mitochondrial membrane-bound enzymes: cytochrome c oxidase and monoamine oxidase A. Together, these results demonstrate that, stabilization of TNIC with BSA opens up the possibility of its practical application in chemotherapy of socially significant diseases.
Molecules, 2020
New hybrids of 4-amino-2,3-polymethylenequinoline with different sizes of the aliphatic ring link... more New hybrids of 4-amino-2,3-polymethylenequinoline with different sizes of the aliphatic ring linked to butylated hydroxytoluene (BHT) by enaminoalkyl (7) or aminoalkyl (8) spacers were synthesized as potential multifunctional agents for Alzheimer’s disease (AD) treatment. All compounds were potent inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with selectivity toward BChE. Lead compound 8c, 2,6-di-tert-butyl-4-{[2-(7,8,9,10- tetrahydro-6H-cyclohepta[b]quinolin-11-ylamino)-ethylimino]-methyl}-phenol exhibited an IC50(AChE) = 1.90 ± 0.16 µM, IC50(BChE) = 0.084 ± 0.008 µM, and 13.6 ± 1.2% propidium displacement at 20 μM. Compounds possessed low activity against carboxylesterase, indicating likely absence of clinically unwanted drug-drug interactions. Kinetics were consistent with mixed-type reversible inhibition of both cholinesterases. Docking indicated binding to catalytic and peripheral AChE sites; peripheral site binding along with propidium displacement...
a Institute of Problems of Chemical Physics Russian Academy of Sciences, 142432, pr. akademika Se... more a Institute of Problems of Chemical Physics Russian Academy of Sciences, 142432, pr. akademika Semenova 1, Chernogolovka, Moscow Region, Russia b A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, 119991, Vavilova St. 28, Moscow, Russia ifaingold@mail.ru The problem of creating contrast agents is of current interest now, because magnetic resonance imaging (MRI) is widely used in medicine for diagnosis of diseases of man. The Institute of Problems of Chemical Physics has developed a method of synthesis of gadofullerenes Gd@C82 in the anionic form, from which new of new water-soluble endometallofullerenes (EMF): Gd@C82(OH)x, Gd@C82-H Pro, Gd@C82-Hydroxyethyl Pro and Gd @C82-Maleimide Pro were obtained by selective modification. This work reports on an investigation of their membranotropic and relaxation properties. An important task of modern physical in chemical biology and pharmacology is the study of the mechanisms of interaction of potential drugs...
Doklady Biochemistry and Biophysics
Bulletin of Experimental Biology and Medicine
Russian Chemical Bulletin
Doklady Physical Chemistry
Doklady Physical Chemistry, 2015
Russian Chemical Bulletin, 2014
Russian Chemical Bulletin, 2014
Russian Chemical Bulletin, 2014
The user has requested enhancement of the downloaded file.
ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2... more ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2(SR′)2(NO)4]2+ with R′ = cysteamine and penicillamine - and of the neutral type - [Fe2(SR″)2(NO)4]0 with R″ = benzthiazoline – on the enzymatic activity of hydrolases – cyclic guanosine monophosphate phosphodiesterase (PDEcGMP) and sarcoplasmic reticulum Ca2+-Mg2+-dependent ATPase (SR-Ca2+-ATPase). All the complexes studied were found to be modulators of both enzymes. They produced weak inhibition of PDEcGMP activity and marked inhibition of active transport by SR Ca2+-ATPase. While having virtually no effect on the hydrolytic center of SR Ca2+-ATPase, the active transport of calcium was completely blocked over the concentration range 1 μM – 0.1 mM, with uncoupling of the hydrolytic and transport functions of this enzyme. This suggests that these complexes can induce structural-functional changes in SR Ca2+-ATPase at concentrations corresponding to an enzyme:inhibitor ratio of 1:1, which is consistent with the antimetastatic action of these compounds.
Nanotechnologies in Russia
Patterns of the interaction between water-soluble polysubstituted fullerene derivatives (PFDs) an... more Patterns of the interaction between water-soluble polysubstituted fullerene derivatives (PFDs) and the lipid bilayer of phosphatidylcholine liposomes were investigated by applying triplet and fluorescent probes. Objective quantitative criteria have been proposed for the evaluation of membranotropic action of chemical substances, notably, fullerene derivatives that quench fluorescent probes with different localizations within the membrane. Thus, the defined criteria are the rate constants for the quenching of the fluorescence of triplet probes and the equilibrium constants for PFD-probe complexes, which characterize their stability. The localization of PFDs in the membrane was determined by comparing rate constants for the quenching of eosin phosphorescence and equilibrium constants for PFD-chromophore complexes. In addition, the efficiency of the interaction of PFDs with various sites of the phospholipid membrane has been seen to depend on the charge of addends that are attached to ...
Russian Chemical Bulletin, 2011
Pharmaceutical Chemistry Journal, 2012
ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2... more ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2(SR′)2(NO)4]2+ with R′ = cysteamine and penicillamine - and of the neutral type - [Fe2(SR″)2(NO)4]0 with R″ = benzthiazoline – on the enzymatic activity of hydrolases – cyclic guanosine monophosphate phosphodiesterase (PDEcGMP) and sarcoplasmic reticulum Ca2+-Mg2+-dependent ATPase (SR-Ca2+-ATPase). All the complexes studied were found to be modulators of both enzymes. They produced weak inhibition of PDEcGMP activity and marked inhibition of active transport by SR Ca2+-ATPase. While having virtually no effect on the hydrolytic center of SR Ca2+-ATPase, the active transport of calcium was completely blocked over the concentration range 1 μM – 0.1 mM, with uncoupling of the hydrolytic and transport functions of this enzyme. This suggests that these complexes can induce structural-functional changes in SR Ca2+-ATPase at concentrations corresponding to an enzyme:inhibitor ratio of 1:1, which is consistent with the antimetastatic action of these compounds.
Membranes
This paper shows the biological effects of cationic binuclear tetranitrosyl iron complex with pen... more This paper shows the biological effects of cationic binuclear tetranitrosyl iron complex with penicillamine ligands (TNIC–PA). Interaction with a model membrane was assessed using a fluorescent probes technique. Antioxidant activity was studied using a thiobarbituric acid reactive species assay (TBARS) and a chemiluminescence assay. The catalytic activity of monoamine oxidase (MAO) was determined by measuring liberation of ammonia. Antiglycation activity was determined fluometrically by thermal glycation of albumine by D-glucose. The higher values of Stern–Volmer constants (KSV) obtained for the pyrene located in hydrophobic regions (3.9 × 104 M−1) compared to KSV obtained for eosin Y located in the polar headgroup region (0.9 × 104 M−1) confirms that TNIC–PA molecules prefer to be located in the hydrophobic acyl chain region, close to the glycerol group of lipid molecules. TNIC–PA effectively inhibited the process of spontaneous lipid peroxidation, due to additive contributions fro...
Mendeleev Communications, 2022
Doklady. Physical chemistry, 2016
The potential of surface enhanced Raman spectroscopy (SERS) for the detection of water-soluble fu... more The potential of surface enhanced Raman spectroscopy (SERS) for the detection of water-soluble fullerene derivatives and their covalent conjugates with xanthene dyes was investigated in model biological liposome membranes and in the albumin protein structure. It was shown that in liposomes and in albumin, fullerene derivatives and their covalent conjugates with dyes show characteristic SERS spectra, which allows detection of water-soluble fullerene derivatives in phosphatidylcholine liposomes at the lipid/fullerene derivative ratio of 100 as well as fullerene–dye conjugates in liposomes and albumin.
Journal of Polymer Research, 2021
The complexes of metformin (MET) and the copolymer of N-vinylpyrrolidone with triethylene glycol ... more The complexes of metformin (MET) and the copolymer of N-vinylpyrrolidone with triethylene glycol dimethacrylate with absolute molecular weight ca. 26 kDa and hydrodynamic radius of macromolecules about 4 nm have been prepared and studied in water buffer solution. Their sizes in neutral phosphate buffer solution depended on MET (10–40 wt%) per the copolymer content, temperature and the copolymer concentrations. Quantum chemical simulation has shown the most energetically favorable and stable structure is one with the coordination of neighboring NH2 groups of MET via oxygen carbonyl of the VP fragment. The effective binding constant of MET with the copolymer estimated from the absorption spectroscopy was found to be ~ 1.8 × 103 M−1. The MET-copolymer compositions were analyzed by IR spectroscopy, thermogravimetry, differential scanning calorimetry, and X-ray diffraction. The copolymer complex with 20 wt% of MET affected blood glucose level in streptozotocin-induced diabetic mice like MET and decreased aldose reductase activity in contrast with the drug and MET-PVP complex.
Nitric Oxide, 2021
Nitric oxide (NO) mediates diverse physiological processes in living organisms. Small molecular N... more Nitric oxide (NO) mediates diverse physiological processes in living organisms. Small molecular NO donors usually lack stability and have a short half-life in human tissues, limiting the therapeutic application. The anionic tetranitrosyl iron complex with thiosulfate ligands (TNIC) is one of the most promising NO donors. This study shows that bovine serum albumin (BSA) can effectively stabilize the TNIC complex under aerobic (physiological) conditions, which contributes to its prolonged action as NO donor. Our results demonstrated that TNIC-BSA inhibits formation of TBARS - standard biomarker for the lipid peroxidation induced oxidative stress. Also, it was found that TNIC-BSA inhibits the catalytic activity of mitochondrial membrane-bound enzymes: cytochrome c oxidase and monoamine oxidase A. Together, these results demonstrate that, stabilization of TNIC with BSA opens up the possibility of its practical application in chemotherapy of socially significant diseases.
Molecules, 2020
New hybrids of 4-amino-2,3-polymethylenequinoline with different sizes of the aliphatic ring link... more New hybrids of 4-amino-2,3-polymethylenequinoline with different sizes of the aliphatic ring linked to butylated hydroxytoluene (BHT) by enaminoalkyl (7) or aminoalkyl (8) spacers were synthesized as potential multifunctional agents for Alzheimer’s disease (AD) treatment. All compounds were potent inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with selectivity toward BChE. Lead compound 8c, 2,6-di-tert-butyl-4-{[2-(7,8,9,10- tetrahydro-6H-cyclohepta[b]quinolin-11-ylamino)-ethylimino]-methyl}-phenol exhibited an IC50(AChE) = 1.90 ± 0.16 µM, IC50(BChE) = 0.084 ± 0.008 µM, and 13.6 ± 1.2% propidium displacement at 20 μM. Compounds possessed low activity against carboxylesterase, indicating likely absence of clinically unwanted drug-drug interactions. Kinetics were consistent with mixed-type reversible inhibition of both cholinesterases. Docking indicated binding to catalytic and peripheral AChE sites; peripheral site binding along with propidium displacement...
a Institute of Problems of Chemical Physics Russian Academy of Sciences, 142432, pr. akademika Se... more a Institute of Problems of Chemical Physics Russian Academy of Sciences, 142432, pr. akademika Semenova 1, Chernogolovka, Moscow Region, Russia b A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, 119991, Vavilova St. 28, Moscow, Russia ifaingold@mail.ru The problem of creating contrast agents is of current interest now, because magnetic resonance imaging (MRI) is widely used in medicine for diagnosis of diseases of man. The Institute of Problems of Chemical Physics has developed a method of synthesis of gadofullerenes Gd@C82 in the anionic form, from which new of new water-soluble endometallofullerenes (EMF): Gd@C82(OH)x, Gd@C82-H Pro, Gd@C82-Hydroxyethyl Pro and Gd @C82-Maleimide Pro were obtained by selective modification. This work reports on an investigation of their membranotropic and relaxation properties. An important task of modern physical in chemical biology and pharmacology is the study of the mechanisms of interaction of potential drugs...
Doklady Biochemistry and Biophysics
Bulletin of Experimental Biology and Medicine
Russian Chemical Bulletin
Doklady Physical Chemistry
Doklady Physical Chemistry, 2015
Russian Chemical Bulletin, 2014
Russian Chemical Bulletin, 2014
Russian Chemical Bulletin, 2014
The user has requested enhancement of the downloaded file.
ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2... more ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2(SR′)2(NO)4]2+ with R′ = cysteamine and penicillamine - and of the neutral type - [Fe2(SR″)2(NO)4]0 with R″ = benzthiazoline – on the enzymatic activity of hydrolases – cyclic guanosine monophosphate phosphodiesterase (PDEcGMP) and sarcoplasmic reticulum Ca2+-Mg2+-dependent ATPase (SR-Ca2+-ATPase). All the complexes studied were found to be modulators of both enzymes. They produced weak inhibition of PDEcGMP activity and marked inhibition of active transport by SR Ca2+-ATPase. While having virtually no effect on the hydrolytic center of SR Ca2+-ATPase, the active transport of calcium was completely blocked over the concentration range 1 μM – 0.1 mM, with uncoupling of the hydrolytic and transport functions of this enzyme. This suggests that these complexes can induce structural-functional changes in SR Ca2+-ATPase at concentrations corresponding to an enzyme:inhibitor ratio of 1:1, which is consistent with the antimetastatic action of these compounds.
Nanotechnologies in Russia
Patterns of the interaction between water-soluble polysubstituted fullerene derivatives (PFDs) an... more Patterns of the interaction between water-soluble polysubstituted fullerene derivatives (PFDs) and the lipid bilayer of phosphatidylcholine liposomes were investigated by applying triplet and fluorescent probes. Objective quantitative criteria have been proposed for the evaluation of membranotropic action of chemical substances, notably, fullerene derivatives that quench fluorescent probes with different localizations within the membrane. Thus, the defined criteria are the rate constants for the quenching of the fluorescence of triplet probes and the equilibrium constants for PFD-probe complexes, which characterize their stability. The localization of PFDs in the membrane was determined by comparing rate constants for the quenching of eosin phosphorescence and equilibrium constants for PFD-chromophore complexes. In addition, the efficiency of the interaction of PFDs with various sites of the phospholipid membrane has been seen to depend on the charge of addends that are attached to ...
Russian Chemical Bulletin, 2011
Pharmaceutical Chemistry Journal, 2012
ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2... more ABSTRACT We report here our studies of new nitrosyl complexes of iron of the cationic type - [Fe2(SR′)2(NO)4]2+ with R′ = cysteamine and penicillamine - and of the neutral type - [Fe2(SR″)2(NO)4]0 with R″ = benzthiazoline – on the enzymatic activity of hydrolases – cyclic guanosine monophosphate phosphodiesterase (PDEcGMP) and sarcoplasmic reticulum Ca2+-Mg2+-dependent ATPase (SR-Ca2+-ATPase). All the complexes studied were found to be modulators of both enzymes. They produced weak inhibition of PDEcGMP activity and marked inhibition of active transport by SR Ca2+-ATPase. While having virtually no effect on the hydrolytic center of SR Ca2+-ATPase, the active transport of calcium was completely blocked over the concentration range 1 μM – 0.1 mM, with uncoupling of the hydrolytic and transport functions of this enzyme. This suggests that these complexes can induce structural-functional changes in SR Ca2+-ATPase at concentrations corresponding to an enzyme:inhibitor ratio of 1:1, which is consistent with the antimetastatic action of these compounds.