DR. KRISHNA KULKARNI - Academia.edu (original) (raw)

Papers by DR. KRISHNA KULKARNI

International journal of engineering research and technology, Nov 15, 2019

International journal of engineering research and technology, 2019

Journal of emerging technologies and innovative research, Dec 1, 2020

Chromatographia, 2001

ABSTRACT A high performance thin layer chromatographic method was developed for the determination... more ABSTRACT A high performance thin layer chromatographic method was developed for the determination of erythromycin. The drug was separated on a silica gel 60 plate and developed in methanol by means of an automatic multiple development. The chromatogram was sprayed with 10% sulphuric acid solution and heated at 100°C for 10–15 minutes. The area of the spot was quantified by a TLC scanner at a wavelength of 410 nm. A linear calibration curve was established over the range of 4–6 μg in 10μl of erythromycin. The relative standard deviation for five replicate determinations was found to be 1.45% for 5 μg in 10 μl of erythromycin standard. The average percentage recovery was found to be 99.87. The method has been applied to the determination of erythromycin in various pharmaceutical dosage forms. Common excipients in formulations do not interfere. After optimizing the solvent system, it was found that the use of silica gel 60 F254 TLC plate with a DVS composed of ethyl acetate, ethanol and 10% sodium acetate pH 9.5 (9:7:8) led to the differentiation and quantitation of erythromycins A, B and C with an R.S.D. of less than 2.0%. The method is simple, precise and inexpensive. It should be used for routine analysis.

Identification of forensic samples by thin layer chromatography (TLC) is the most widely used sep... more Identification of forensic samples by thin layer chromatography (TLC) is the most widely used separation technique. Modern analytical instrumental methods such as spectophotometry, gas- chromatography, HPLC, HPTLC, and mass spectrometry are sensitive and they are widely used in forensic analysis.The identification of organophosphorous, organochlro, carbamate and pyrethroid insecticidespesticide like avant (Indocacarb), monocrotophos, carbosulfan, hydrogen cynamide, tridemorph and pyrethroid insecticide like cypermethrin. Consequently characterization of these pesticide is necessary in forensic case work, their identification in biological material (viscera, stomach wash, blood, etc.) and non-biological material (material found at the site of scene of crime e.g. container of pesticide, strip of drugs, syringes, injection, cloths, etc.) is important task in forensic case work.

JPC - Journal of Planar Chromatography - Modern TLC, 2016

Anuran amphibians are the biological models to assess the influence of environmental contaminatio... more Anuran amphibians are the biological models to assess the influence of environmental contamination. We conducted nuclear abnormality assessment and micronuclei test in erythrocytes of frogs to identify an early influence of environmental contaminations. In Western Ghats of India, farmers use different agrochemicals and obviously, the amphibian habitat is contaminated with combinations of many residues. Many frog species use these agro-ecosystem for breeding and to complete early life stage. In the present study, we used Indian skipper frog (Euphlyctis cyanophlyctis (Anura: Ranidae)), a common inhabitant of water bodies in agro-ecosystems of Western Ghats for the assay. We collected the adult frogs from three different habitats; a) the rice paddy fields contaminated with agrochemicals, b) uncontaminated marsh and c) a shallow water pool. We recorded micronucleus, blebbed, lobed, kidney bean shaped nucleus and nucleus with notches in erythrocytes of these frogs. These nuclear abnormal...

Child: Care, Health and Development, 2020

Abstract Amidst the coronavirus disease 2019 (COVID‐19) pandemic, there is uncertainty regarding ... more Abstract Amidst the coronavirus disease 2019 (COVID‐19) pandemic, there is uncertainty regarding potential lasting impacts on children's health and educational outcomes. Play, a fundamental part of childhood, may be integral to children's health during crises. We undertook a rapid review of the impact of quarantine, isolation and other restrictive environments on play and whether play mitigates adverse effects of such restrictions. Fifteen peer‐reviewed studies were identified, spanning hospitals, juvenile and immigration detention and refugee camps. We found evidence of changes in children's access to play in crises and quarantine. These studies indicated how play might support children enduring isolation but lacked robust investigations of play as an intervention in mitigating impacts of restriction. Studies pertaining to children in isolation due to infectious disease outbreaks were notably absent. It is important that the potential effects of changes to such a crucial aspect of childhood are better understood to support children in this and future crises.

In the wake of the current COVID-19 health crisis, there is uncertainty and concern about the imp... more In the wake of the current COVID-19 health crisis, there is uncertainty and concern about the impact this pandemic will have on children’s health and educational outcomes. Play is a fundamental part of childhood and can be integral to children’s health in moments of crisis. Due to severe lockdown regulations around the world, typical play experiences have needed to adapt to school and playground closures, changes to peer interactions, and social distancing. We undertook a rapid literature review of the impact of quarantine, isolation, or other restrictive environments on children’s play and whether play may mitigate the adverse effects of such restrictions. Fifteen peer-reviewed studies were identified, spanning various environments in which children faced restriction, including hospitalisation, juvenile and immigration detention, and displacement to a refugee camp. We found that the literature provided evidence of changes in children’s access to play under restrictive circumstances...

International Journal of Play, 2019

This paper provides an overview of the work being conducted at the Play in Education, Development... more This paper provides an overview of the work being conducted at the Play in Education, Development and Learning (PEDAL) Research Centre, based in the Faculty of Education, University of Cambridge, UK. PEDAL has three main aims, (1) To conduct worldclass research, (2) To build capacity in play research and (3) To influence policy and practice. The present paper provides an overview of the history and rationale for PEDAL, followed by some detailed examples of the foci of its work. Exemplar research is presented on the theme of 'Measuring Play'; an endeavour that is common to many of the different research projects led by PEDAL investigators and Ph.D. students.

This paper deals with the use of high Performance Thin layer Chromatography with sensitive spray ... more This paper deals with the use of high Performance Thin layer Chromatography with sensitive spray reagents for detection and identification of poisonous plants in autopsy tissues. This laboratory has received human as well as cattle cases with history of death due to ingestion of poisonous plant material in various forms. There can be legal quarries regarding detection and identification of these poisonous plants, which a forensic expert has to answer. In the present paper, a very little effort has been taken to provide a perfect database for forensic toxicologist which can be useful for detection of poisonous plant.

High performance thin layer chromatography has found wide recognition in many fields and in sensi... more High performance thin layer chromatography has found wide recognition in many fields and in sensitivity of detection offers particular advantage to the toxicologist, which has increased, 10 to 100 times as compared to the chemical method. It has become an important analytical tool since it can separate complex mixture in a relatively short time. In existing study, an effort has been taken to determine organ phosphorus insecticide Acephate by using high performance thin layer chromatography. A new specific Sensitive chromogenic reagent 0.1% solution of ferric chloride in 80% ethanol and 1 % Sulfosalicylic acid in 80% ethanol has been developed for detection of Acephate an organophosphorus insecticide with solvent system petroleum ether: methanol (95: 5).

JPC - Journal of Planar Chromatography - Modern TLC, 2009

... A New Chromogenic Spray Reagent for Detection and Identification of Monocrotophos Vijay R. Ch... more ... A New Chromogenic Spray Reagent for Detection and Identification of Monocrotophos Vijay R. Chandegaonkar, Devanand B. Shinde, and Dhananjay V. Mane* Key Words ... References [1] GB Kawale, VD Joglekar, VP Barve, and HS Mahal, Sci. Cult. 38 (1972) 373. ...

IBM Systems Journal, 2006

Relational database systems have dominated the database industry for a quarter century. However, ... more Relational database systems have dominated the database industry for a quarter century. However, the advent of the Web has led to requirements for storage of new kinds of information in which the order of information is important and data structure can vary over time and from one document to another. These evolving requirements have given rise to Extensible Markup Language (XML) as a widely accepted data format and to XQuery as an emerging standard language for querying XML data sources. A set of extensions to the Structured Query Language (SQL) called SQL/XML enables XML data to be stored in relational databases, taking advantage of the mature infrastructure of relational systems and combining the advantages of SQL and XQuery. However, building a bridge between SQL and XQuery is challenging due to the many syntactic and semantic differences between the two languages. This paper describes how IBM DB2t deals with this challenge and provides users with a flexible system for storing and processing both relational and XML data.

Journal of emerging technologies and innovative research, 2020

Abstract: The objective of this project is to design an efficient automatic authorized vehicle id... more Abstract: The objective of this project is to design an efficient automatic authorized vehicle identification system using RFID. Due to rise in the number of accidents and violation of signal rules, we have developed this system which would prevent the problem and thus create a safe crossing for the pedestrian as well as for people travelling in vehicles.. In the developed system, when the signal is red and vehicle is on zebra crossing line, then the system detects the RFID Tag situated on vehicle through RFID Reader. Once the unique Tag number is read by the RFID Reader it compares the Tag number with records on a database. In database there can be specific information about the vehicle and vehicle owner. If the Tag number matches with the database then the associated owners information is verified and then displayed. After retrieval of owners details the RTO has authority to send notification and apply fine to the correspondent owner and thus owner too has authority to view histor...