Dacheng Liang - Academia.edu (original) (raw)

Papers by Dacheng Liang

International Journal of Molecular Sciences, 2021

Root-derived mobile signals play critical roles in coordinating a shoot’s response to underground... more Root-derived mobile signals play critical roles in coordinating a shoot’s response to underground conditions. However, the identification of root-to-shoot long-distance mobile signals has been scant. In this study, we aimed to characterize root-to-shoot endogenous mobile miRNAs by using an Arabidopsis/Nicotiana interfamilial heterograft in which these two taxonomically distant species with clear genetic backgrounds had sufficient diversity in differentiating miRNA sources. Small RNA deep sequencing analysis revealed that 82 miRNAs from the Arabidopsis scion could travel through the graft union to reach the rootstock, whereas only a very small subset of miRNA (6 miRNAs) preferred the root-to-shoot movement. We demonstrated in an ex vivo RNA imaging experiment that the root-to-shoot mobile Nb-miR164, Nb-miR395 and Nb-miR397 were targeted to plasmodesmata using the bacteriophage coat protein MS2 system. Furthermore, the Nb-miR164 was shown to move from the roots to the shoots to induce...

Frontiers in Plant Science

Scion-rootstock union formation is a critical step toward the functional assemblage of heterogene... more Scion-rootstock union formation is a critical step toward the functional assemblage of heterogeneous plants. Interfamilial scion-rootstock interaction often results in graft incompatibility during the assemblage process, and the underlying mechanisms are largely unknown. In this study, we reported that tracheary element (TE) remodeling, including TE segmentation and deformation, rather than de novo formation from callus or adjacent tissues, took place at the early stage of grafting interface between Arabidopsis thaliana and Nicotiana benthamiana (At/Nb). Following cellular deposits, the short TEs from both partners were overlapping, dependent on the homogeneity of contacting TEs, with each other. Without overlapping, the TEs at the interface would grow laterally, and the TEs above and below the interface would undergo self-fusion to form insulating spiraling bundles. Finally, the overlapping TEs constituted a continuous network through alignment. Our results provide a definitive fra...

Journal of Visualized Experiments

The symplastic tracer 5(6)-carboxyfluorescein diacetate (CFDA) has been widely applied in living ... more The symplastic tracer 5(6)-carboxyfluorescein diacetate (CFDA) has been widely applied in living plants to demonstrate the intercellular connection, phloem transport and vascular patterning. This protocol shows bottom-to-top carboxyfluorescein (CF) movement in the Arabidopsis by using the root-cutting and the hypocotyl-pinching procedure respectively. These two different procedures result in different efficiencies of CF movement: about 91% appearance of CF in the shoots with the hypocotyl-pinching procedure, whereas only about 70% appearance of CF with the root-cutting procedure. The simple change of loading sites, resulting in significant changes in the mobile efficiency of this symplastic dye, suggests CF movement might be subject to the symplastic regulation, most probably by the root-hypocotyl junction.

Pakistan Journal of Botany

Frontiers in Cell and Developmental Biology

Background Scion–rootstock union formation is a critical step towards functional assemblage of he... more Background Scion–rootstock union formation is a critical step towards functional assemblage of heterogeneous plants. However, scion-rootstock interaction often results in graft incompatibility during the process of assemblage. So far, the lack of model heterografts involving both clear genetic backgrounds and taxonomically distant species greatly impedes insights into the mechanisms underlying scion-rootstock interaction. Results In this report, we established an Arabidopsis (At)/Nicotiana benthamiana (Nb) heterografting system in which the model plant At and the model plant Nb for plant bioreactor was used as scion and rootstock respectively, to explore the interaction between the two model plants. Regarding to the At scion phenotypes, the At-Nb connection can be characterized into three groups: the mild-stressed, the albino and the dormant grafts. Examination of symplastic and apoplastic flow indicated that a functional inter-order grafting was established in the mild-stressed gro...

Trends in Plant Science, 2011

PeerJ, 2014

In plants and nematodes, RNAi can spread from cells from which it is initiated to other cells in ... more In plants and nematodes, RNAi can spread from cells from which it is initiated to other cells in the organism. The underlying mechanism controlling the mobility of RNAi signals is not known, especially in the case of plants. A genetic screen designed to recover plants impaired in the movement but not the production or effectiveness of the RNAi signal identified RCI3, which encodes a hydrogen peroxide (H2O2)-producing type III peroxidase, as a key regulator of silencing mobility in Arabidopsis thaliana. Silencing initiated in the roots of rci3 plants failed to spread into leaf tissue or floral tissue. Application of exogenous H2O2 reinstated the spread in rci3 plants and accelerated it in wild-type plants. The addition of catalase or MnO2, which breaks down H2O2, slowed the spread of silencing in wild-type plants. We propose that endogenous H2O2, under the control of peroxidases, regulates the spread of gene silencing by altering plasmodesmata permeability through remodelling of loca...