Dale Blevins - Academia.edu (original) (raw)

Papers by Dale Blevins

Agronomy Journal, May 1, 1978

Plant Physiology, Jul 1, 1974

The influence of NO<-uptake and reduction on ionic balance in barley seedlings (Hordeumn vulgare,... more The influence of NO<-uptake and reduction on ionic balance in barley seedlings (Hordeumn vulgare, cv. Compana) was studied. KNO3 and KCI treatment solutions were used for comparison of cation and anion uptake. The rate of Cl-uptake was more rapid than the rate of NO3-uptake during the first 2 to 4 hours of treatment. There was an acceleration in rate of NOuptake after 4 hours resulting in a sustained rate of NO3-uptake which exceeded the rate of Cl-uptake. The initial (2 to 4 hours) rate of K+ uptake appeared to be independent of the rate of anion uptake. After 4 hours the rate of K+ uptake was greater with the KNO3 treatnient than with the KCI treatment, and the solution pH, cell sap pH, and organic acid levels with KNO3 increased, relative to those with the KCI treatment. When absorption experiments were conducted in darkness, K+ uptake fron KNO3 did not exceed K+ uptake from KCI. We suggest that the greater uptake and accumulation of K+ in NO3-treated plants resulted from (a) a more rapid, sustained uptake and transport of NO3providing a mobile counteranion for K+ transport, and (b) the synthesis of organic acids in response to NO;reduction increasing the capacity for K+ accumulation by providing a source of nondiffusible organic anions.

Soil Science Society of America Journal, 1983

ASSA, CSSA and SSSA, Nov 2, 2015

Plant Physiology, May 1, 2002

ASSA, CSSA and SSSA, Nov 2, 2015

Green beans and spinach were grown in field plots in a 2 3 x 3 factorially arranged split plot de... more Green beans and spinach were grown in field plots in a 2 3 x 3 factorially arranged split plot design to study the effect of soil fertility treatments on the chemical composition of the edible plant parts. Two levels each of lime, phosphorus, and potassium and three levels of nitrogen were established on a Mexico silt loam soil that was initially low in fertility. No green bean yield response from the treatment was obtained because of pre-planting management and weather. Significant yield increases of spinach were obtained from all main treatments ; nutrient interaction effects on yield were also observed. N0 3-N, Ca, K, Mn, Zn, Mg, Na, and P concentrations in the edible parts were determined. The concentrations of most of these elements in both vegetables were affected by soil treatment. The net effect of any one soil treatment depended on the levels of the other treatments.

Plant and Soil

Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacit... more Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacity in response to iron (Fe) deficiency stress. In squash (Cucurbita pepo L.) seedlings, Fe(III) reducing capacity was quantified during early vegetative growth. When plants were grown in Fe-free solution, the Fe(III) reducing capacity was greatly elevated, reached peak activity on day 4, then declined through day 6. Root ethylene production exhibited a temporal pattern that closely matched that of Fe(III) reducing capacity through day 6. On the 7th day of Fe deficiency, cluster root morphology developed, which coincided with a sharp increase in the root Fe(III) reducing capacity, although ethylene production decreased. Localization of Fe(III) reducing capacity activity was observed during the onset of Fe deficiency and through the development of the root clusters. It was noted that localization shifted from an initial pattern which occurred along the main and primary lateral root axes, ex...

Plant Nitrogen Metabolism, 1989

The predominant sources of nitrogen for higher plants are NH4 +, NO3 - and N2. In most forests, w... more The predominant sources of nitrogen for higher plants are NH4 +, NO3 - and N2. In most forests, where soils are acidic and low in nitrogen, NH4 + is the major nitrogen source. In agricultural soils, NO3 - is the primary source of nitrogen for plant growth and development, even though NH4 + or urea fertilizers may have been applied. Soil microorganisms, under normal conditions, oxidize the NH4 + or urea-N to NO3 - by the process of nitrification. In this review, the metabolism of nitrogen obtained by higher plants from NH4 +, N03 -; or N2 will be discussed.

PLANT PHYSIOLOGY, 1976

Levels of nitrate reductase activity (EC 1.9.6.1.) as high as 11 ,lmoles nitrite produced/hour gr... more Levels of nitrate reductase activity (EC 1.9.6.1.) as high as 11 ,lmoles nitrite produced/hour gram fresh weight were found in barley (Hordeum vulgare cv. Compana) roots grown under low oxygen conditions. Roots of plants given identical treatment under sterile conditions did not develop the high levels of nitrate reductase activity. The results suggest that the buildup of particulate, reduced viologen-utilizing nitrate reductase reported in barley roots may be caused by bacterial contamination. The nitrate reductase activity in roots grown under low oxygen conditions was not specific for reduced nicotinamide adenine dinucleotide like the assmilatory nitrate reductase (EC 1.6.6.1.) normally found in aerated plant roots.

Plant Physiology, 1987

ABSTRACr MATERIAIS AND METHODS Under certain sss citions roots exude orgnic molecules, which my f... more ABSTRACr MATERIAIS AND METHODS Under certain sss citions roots exude orgnic molecules, which my faciitate the uptake of e objective of this resenrc was to identify and measue the effect of low P upon the exudation of orgaic adds by roots of alfalfa (Medceg shat L) eings Surface-sterilized alfalfa seeds were grown asepficayll in sterile sand using an appartus specially desiWed for the addition of ±P nutient sointions and for the cdoction of root exudates. Citric, maric, and succnc acids were detected in the root exudates of 24-day-old alfaf seedings. Citrate exudation from the roots of P-stressed alflfa was 182% that of plants receiving a Complete nutrient sonton rela of citrate my provide a mecis by whic P-stressed plants enhance the avaiblity of P in the rhizosp_ere.

Plant and Soil, 1981

SummaryOnly legumes of the cowpea cross-inoculation group, including the winged bean (Psophocarpu... more SummaryOnly legumes of the cowpea cross-inoculation group, including the winged bean (Psophocarpus tetragonolobus) were found to form nodules in a temperate zone soil with no previous history of legume cropping. Isolates from root nodules from uninoculated winged beans grown in the field only nodulated legumes in the cowpea cross-inoculation group.Rhizobium japonicum formed ineffective nodules with the winged bean.

Journal of Plant Nutrition, 1995

... isolated from roots. Auteur(s) / Author(s). BLEVINS DG ; DROPKIN VH ; LUEDDERS VD ; Affiliati... more ... isolated from roots. Auteur(s) / Author(s). BLEVINS DG ; DROPKIN VH ; LUEDDERS VD ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). Univ. Missouri, plant sci. unit, Columbia MO 65211, ETATS-UNIS Résumé / Abstract. ...

These proceedings represent papers presented at the Fifth Annual Plant Biochemistry and Physiolog... more These proceedings represent papers presented at the Fifth Annual Plant Biochemistry and Physiology Symposium held at the University of Missouri-Columbia, April 2-4, 1986, and hosted by the Interdisciplinary Plant Biochemistry and Physiology Group. The Interdisciplinary Group was organized to facilitate research and training through interdisciplinary and cooperative approaches to problems facing Plant Biochemistry and Physiology. This annual symposium is a

The Proceedings of the International Plant Nutrition Colloquium Xvi, Apr 10, 2009

Archives of Biochemistry and Biophysics, Apr 1, 1983

Xanthine dehydrogenase was purified from soybean nodules and the kinetic properties were studied ... more Xanthine dehydrogenase was purified from soybean nodules and the kinetic properties were studied at pH 7.5. K, values of 5.0 + 0.6 and 12.5 + 2.5 PM were obtained for xanthine and NAD+, respectively. The pattern of substrate dependence suggested a Ping-Pong mechanism. Reaction with hypoxanthine gave K,'s of 52 + 3 and 20 f 2.5 PM for hypoxanthine and NAD+, respectively. The V,,, for this reaction was twice that for the xanthine-dependent reaction. The pH dependence of V,,, gave a pK, of 7.6 f 0.1 for either xanthine or hypoxanthine oxidation. In addition the K, for xanthine had a pK, of 7.5 consistent with the protonated form of xanthine being the true substrate. K, for hypoxanthine varied only 2.5-fold between pH 6 and 10.7. Product inhibition studies were carried out with urate and NADH. Both products gave mixed inhibition with respect to both substrates. Xanthine dehydrogenase was able to use APAD+ as an electron acceptor for xanthine oxidation, with a K, at pH 7.5 of 21.2 +-2.5 I.

The peroxisomal NADH-hydroxypyruvate reductase (HPR-I) is one of a series of reactions comprising... more The peroxisomal NADH-hydroxypyruvate reductase (HPR-I) is one of a series of reactions comprising the photorespiratory pathway (1). The enzyme prefers NADH and hydroxypyruvate as substrates, but uses NADPH and glyoxylate (Km = 20 mM) rather inefficiently (2). Besides HPR-I, leaves contain a specific glyoxylate reductase (Gr-I) which utilizes only NADPH (3) and is localized in chloroplasts (4). The hydroxypyruvate- or glyoxylate-dependent rates with NADH and/or NADPH reported for crude leaf extracts have usually been attributed to either NADH-HPR-I or NADPH-GR-I or both of these enzymes. We report here the identification and partial characterization of two novel reductases utilizing hydroxypyruvate and/or glyoxylate as substrates and prefer NADPH as a cofactor. This is the first report describing leaf NADPH(NADH)HPR-II, while the purification and some characteristics of the second new reductase, NADPH(NADH)-Gr-II, have already been published (5).

Forage and Grazinglands, 2011

Forage and Grazinglands, 2010

Agronomy Journal, May 1, 1978

Plant Physiology, Jul 1, 1974

The influence of NO<-uptake and reduction on ionic balance in barley seedlings (Hordeumn vulgare,... more The influence of NO<-uptake and reduction on ionic balance in barley seedlings (Hordeumn vulgare, cv. Compana) was studied. KNO3 and KCI treatment solutions were used for comparison of cation and anion uptake. The rate of Cl-uptake was more rapid than the rate of NO3-uptake during the first 2 to 4 hours of treatment. There was an acceleration in rate of NOuptake after 4 hours resulting in a sustained rate of NO3-uptake which exceeded the rate of Cl-uptake. The initial (2 to 4 hours) rate of K+ uptake appeared to be independent of the rate of anion uptake. After 4 hours the rate of K+ uptake was greater with the KNO3 treatnient than with the KCI treatment, and the solution pH, cell sap pH, and organic acid levels with KNO3 increased, relative to those with the KCI treatment. When absorption experiments were conducted in darkness, K+ uptake fron KNO3 did not exceed K+ uptake from KCI. We suggest that the greater uptake and accumulation of K+ in NO3-treated plants resulted from (a) a more rapid, sustained uptake and transport of NO3providing a mobile counteranion for K+ transport, and (b) the synthesis of organic acids in response to NO;reduction increasing the capacity for K+ accumulation by providing a source of nondiffusible organic anions.

Soil Science Society of America Journal, 1983

ASSA, CSSA and SSSA, Nov 2, 2015

Plant Physiology, May 1, 2002

ASSA, CSSA and SSSA, Nov 2, 2015

Green beans and spinach were grown in field plots in a 2 3 x 3 factorially arranged split plot de... more Green beans and spinach were grown in field plots in a 2 3 x 3 factorially arranged split plot design to study the effect of soil fertility treatments on the chemical composition of the edible plant parts. Two levels each of lime, phosphorus, and potassium and three levels of nitrogen were established on a Mexico silt loam soil that was initially low in fertility. No green bean yield response from the treatment was obtained because of pre-planting management and weather. Significant yield increases of spinach were obtained from all main treatments ; nutrient interaction effects on yield were also observed. N0 3-N, Ca, K, Mn, Zn, Mg, Na, and P concentrations in the edible parts were determined. The concentrations of most of these elements in both vegetables were affected by soil treatment. The net effect of any one soil treatment depended on the levels of the other treatments.

Plant and Soil

Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacit... more Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacity in response to iron (Fe) deficiency stress. In squash (Cucurbita pepo L.) seedlings, Fe(III) reducing capacity was quantified during early vegetative growth. When plants were grown in Fe-free solution, the Fe(III) reducing capacity was greatly elevated, reached peak activity on day 4, then declined through day 6. Root ethylene production exhibited a temporal pattern that closely matched that of Fe(III) reducing capacity through day 6. On the 7th day of Fe deficiency, cluster root morphology developed, which coincided with a sharp increase in the root Fe(III) reducing capacity, although ethylene production decreased. Localization of Fe(III) reducing capacity activity was observed during the onset of Fe deficiency and through the development of the root clusters. It was noted that localization shifted from an initial pattern which occurred along the main and primary lateral root axes, ex...

Plant Nitrogen Metabolism, 1989

The predominant sources of nitrogen for higher plants are NH4 +, NO3 - and N2. In most forests, w... more The predominant sources of nitrogen for higher plants are NH4 +, NO3 - and N2. In most forests, where soils are acidic and low in nitrogen, NH4 + is the major nitrogen source. In agricultural soils, NO3 - is the primary source of nitrogen for plant growth and development, even though NH4 + or urea fertilizers may have been applied. Soil microorganisms, under normal conditions, oxidize the NH4 + or urea-N to NO3 - by the process of nitrification. In this review, the metabolism of nitrogen obtained by higher plants from NH4 +, N03 -; or N2 will be discussed.

PLANT PHYSIOLOGY, 1976

Levels of nitrate reductase activity (EC 1.9.6.1.) as high as 11 ,lmoles nitrite produced/hour gr... more Levels of nitrate reductase activity (EC 1.9.6.1.) as high as 11 ,lmoles nitrite produced/hour gram fresh weight were found in barley (Hordeum vulgare cv. Compana) roots grown under low oxygen conditions. Roots of plants given identical treatment under sterile conditions did not develop the high levels of nitrate reductase activity. The results suggest that the buildup of particulate, reduced viologen-utilizing nitrate reductase reported in barley roots may be caused by bacterial contamination. The nitrate reductase activity in roots grown under low oxygen conditions was not specific for reduced nicotinamide adenine dinucleotide like the assmilatory nitrate reductase (EC 1.6.6.1.) normally found in aerated plant roots.

Plant Physiology, 1987

ABSTRACr MATERIAIS AND METHODS Under certain sss citions roots exude orgnic molecules, which my f... more ABSTRACr MATERIAIS AND METHODS Under certain sss citions roots exude orgnic molecules, which my faciitate the uptake of e objective of this resenrc was to identify and measue the effect of low P upon the exudation of orgaic adds by roots of alfalfa (Medceg shat L) eings Surface-sterilized alfalfa seeds were grown asepficayll in sterile sand using an appartus specially desiWed for the addition of ±P nutient sointions and for the cdoction of root exudates. Citric, maric, and succnc acids were detected in the root exudates of 24-day-old alfaf seedings. Citrate exudation from the roots of P-stressed alflfa was 182% that of plants receiving a Complete nutrient sonton rela of citrate my provide a mecis by whic P-stressed plants enhance the avaiblity of P in the rhizosp_ere.

Plant and Soil, 1981

SummaryOnly legumes of the cowpea cross-inoculation group, including the winged bean (Psophocarpu... more SummaryOnly legumes of the cowpea cross-inoculation group, including the winged bean (Psophocarpus tetragonolobus) were found to form nodules in a temperate zone soil with no previous history of legume cropping. Isolates from root nodules from uninoculated winged beans grown in the field only nodulated legumes in the cowpea cross-inoculation group.Rhizobium japonicum formed ineffective nodules with the winged bean.

Journal of Plant Nutrition, 1995

... isolated from roots. Auteur(s) / Author(s). BLEVINS DG ; DROPKIN VH ; LUEDDERS VD ; Affiliati... more ... isolated from roots. Auteur(s) / Author(s). BLEVINS DG ; DROPKIN VH ; LUEDDERS VD ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). Univ. Missouri, plant sci. unit, Columbia MO 65211, ETATS-UNIS Résumé / Abstract. ...

These proceedings represent papers presented at the Fifth Annual Plant Biochemistry and Physiolog... more These proceedings represent papers presented at the Fifth Annual Plant Biochemistry and Physiology Symposium held at the University of Missouri-Columbia, April 2-4, 1986, and hosted by the Interdisciplinary Plant Biochemistry and Physiology Group. The Interdisciplinary Group was organized to facilitate research and training through interdisciplinary and cooperative approaches to problems facing Plant Biochemistry and Physiology. This annual symposium is a

The Proceedings of the International Plant Nutrition Colloquium Xvi, Apr 10, 2009

Archives of Biochemistry and Biophysics, Apr 1, 1983

Xanthine dehydrogenase was purified from soybean nodules and the kinetic properties were studied ... more Xanthine dehydrogenase was purified from soybean nodules and the kinetic properties were studied at pH 7.5. K, values of 5.0 + 0.6 and 12.5 + 2.5 PM were obtained for xanthine and NAD+, respectively. The pattern of substrate dependence suggested a Ping-Pong mechanism. Reaction with hypoxanthine gave K,'s of 52 + 3 and 20 f 2.5 PM for hypoxanthine and NAD+, respectively. The V,,, for this reaction was twice that for the xanthine-dependent reaction. The pH dependence of V,,, gave a pK, of 7.6 f 0.1 for either xanthine or hypoxanthine oxidation. In addition the K, for xanthine had a pK, of 7.5 consistent with the protonated form of xanthine being the true substrate. K, for hypoxanthine varied only 2.5-fold between pH 6 and 10.7. Product inhibition studies were carried out with urate and NADH. Both products gave mixed inhibition with respect to both substrates. Xanthine dehydrogenase was able to use APAD+ as an electron acceptor for xanthine oxidation, with a K, at pH 7.5 of 21.2 +-2.5 I.

The peroxisomal NADH-hydroxypyruvate reductase (HPR-I) is one of a series of reactions comprising... more The peroxisomal NADH-hydroxypyruvate reductase (HPR-I) is one of a series of reactions comprising the photorespiratory pathway (1). The enzyme prefers NADH and hydroxypyruvate as substrates, but uses NADPH and glyoxylate (Km = 20 mM) rather inefficiently (2). Besides HPR-I, leaves contain a specific glyoxylate reductase (Gr-I) which utilizes only NADPH (3) and is localized in chloroplasts (4). The hydroxypyruvate- or glyoxylate-dependent rates with NADH and/or NADPH reported for crude leaf extracts have usually been attributed to either NADH-HPR-I or NADPH-GR-I or both of these enzymes. We report here the identification and partial characterization of two novel reductases utilizing hydroxypyruvate and/or glyoxylate as substrates and prefer NADPH as a cofactor. This is the first report describing leaf NADPH(NADH)HPR-II, while the purification and some characteristics of the second new reductase, NADPH(NADH)-Gr-II, have already been published (5).

Forage and Grazinglands, 2011

Forage and Grazinglands, 2010