Dalia Kheirallah - Academia.edu (original) (raw)

Papers by Dalia Kheirallah

The rise of urbanism is responsible for numerous environmental changes including pollution. Pimel... more The rise of urbanism is responsible for numerous environmental changes including pollution. Pimelia latreillei was used as a biomonitoring insect inhabiting a polluted site at Zawya Abd El-Qader, Alexandria, Egypt that harbor many industrial activities. A significant elevation in metals percentages in soil samples and midgut tissues of beetles sampled from the polluted site compared to the reference site (garden of faculty of science, Alexandria, Egypt) was detected by using energy-dispersive X-ray mi-croanalysis. The biochemical parameters of oxida-tive stress have been affected by pollution. A significant increase in the activity level of Malon-dialde-hyde (MDA) was recorded, while there was a significant decrease in glutathione-S-transferase (GST), ascorbate peroxidase (APOX), acetylcholinesterase (AChE), superoxide dismutase (SOD) and catalase (CAT) activities. As an indication for genotoxicity, a significant increase in the expression levels of metallothionein (PlMT1) was observed in in beetle's midgut tissues sampled from the polluted site. His-tological and ultrastructure alterations were also observed in the midgut tissues of beetles sampled from the polluted site compared to the reference site. Disruption at the cellular and subcellular levels was recorded .

Original article KHEIRALLAH D.A., EL-SAMAD L.M. 2019. Oogenesis anomalies induced by heavy metal ... more Original article KHEIRALLAH D.A., EL-SAMAD L.M. 2019. Oogenesis anomalies induced by heavy metal contamination in two tenebrionid beetles (Blaps polycresta and Trachyderma hispida). Folia Biologica (Kraków) 67: 9-23. Histological and ultrastructure analysis of the ovaries of Blaps polycresta and Trachyderma hispida were performed to illuminate the effect of industrial pollution from a plastic factory located in Khorshed district, Alexandria, Egypt. Detection of heavy metals in the ovarian tissues was achieved by using X-ray microanalysis. The study revealed various anomalies in oocytes and trophocytes. These basic anomalies included exfoliation and vacuolation of follicular epithelium, vacuolated trophocyst, nuclear abnormalities and morphological changes in cytoplasmic organelles. Conclusively, the study was initiated to assess the extent of reproductive corruptions induced by industrial pollution in two insects as a biomarker of exposure which may impair reproduction in humans.

The expression levels of Esterase Isoenzyme and total soluble protein fractionation were studied ... more The expression levels of Esterase Isoenzyme and total soluble protein fractionation were studied in two coleopteran insects Blaps polycresta and Trachyderma hispida to evaluate the possible hazards from ceramic and plastic factories in the Khorshed Region , East of Alexandria, Egypt. Two insect collection sites were selected. The first site was the garden of the Faculty of Science, Moharram Bek, Alexandria University , which is considered a non-polluted site, and Khorshed district, considered as the polluted site. Percentages of heavy metals were estimated using SEM-X-ray microanalysis of soft tissues of both sexes of the two coleopteran insects. Esterase Isoenzymes were found to be overexpressed in B. Polycresta but not T. hispida. Female B. polycresta from the polluted site exhibited overexpression of the second and third loci. Furthermore , the females were found to be more affected than males, which only showed the overexpression of the second loci. T. hispida (females and males) collected from the reference site were found to have increased esterase activity compared with those sampled from the polluted site. The Snake-Skin™ Dialysis tubing technique , used for optimizing the protein extraction method , reflected the highest quantified proteins compared to other, traditional methods. SDS polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the whole-body protein reflected definite variations between T. hispida and B. polycresta in fraction number and activity at the two sites. Varied expression levels for metallothionein (MT) heavy metal resistance proteins for B. polycresta and T. hispida were also detected in the study. Based on these results, we suggest that biochemical biomarkers could infer environmental hazards, B. polycresta and T. hispida are successful biomarkers for heavy metal pollution.