Elisabetta Damiani - Academia.edu (original) (raw)
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Papers by Elisabetta Damiani
International Journal of Pharmaceutics, 2012
Photostability of suncare products is a great area of interest since several sunscreens on the ma... more Photostability of suncare products is a great area of interest since several sunscreens on the market are photounstable, and this is primarily a problem concerning the UVA region (320-400 nm). Here we report a comparative study on the photostability assessment of two commercial sunscreens with same SPF, spread onto glass plates or onto full thickness pig ear skin or human/pig SCE membranes, and exposed to 183 kJ/m(2) UVA. Absorbance spectra and lipid peroxidation (measured by TBARS production) were determined. The results indicate: (a) sunscreen performance consequent to UVA exposure is independent of whether it is spread onto a non-biological and chemically inert substrate such as glass, or on biological substrates such as skin/SCE membranes; (b) despite the same SPF, sunscreen performance and photostability can be very different; (c) the data on human SCE membranes are similar to those on pig SCE membranes, indicating the suitability of the latter as a model for human skin. However, since the results obtained using skin membranes, akin to the more realistic conditions of use in vivo, do not substantially differ from those obtained on glass plates, the method proposed here using the latter may be applied for rapid, inexpensive, efficacy screening of photostability of sunscreens. Photostability testing should be a mandatory requirement for safer sunscreen protection products, since the results clearly show that some are still far from perfect.
International Journal of Pharmaceutics, 2015
Nanostructured lipid carriers (NLC) are widely used for topical delivery of active ingredients in... more Nanostructured lipid carriers (NLC) are widely used for topical delivery of active ingredients into the skin for both local and systemic treatment. But concerns have been raised regarding their potential nanotoxicity. To understand the role of NLC composition in terms of cytotoxicity and pro-oxidant effects, we investigated cell viability and intracellular levels of ROS (reactive oxygen species) production in human dermal fibroblasts (HDF) incubated with five NLC formulations differing in their solid lipid composition. HDF and NLC were also exposed to UVA irradiation in order to evaluate the behavior of NLC under realistic environmental conditions which might promote their instability. Using the Guava via-count assay, all nanoparticles, except for those formulated with Compritol 888 ATO, showed a significant decrease in live cells and a parallel increase in apoptotic or dead cells compared to the control, either before and/or after UVA irradiation (18J/cm(2)). NLC formulated with Geleol™ Mono Diglycerides resulted the most cytotoxic. A similar trend was also observed when intracellular ROS levels were measured in HDF incubated with NLC: there was increased ROS content compared to the control, further exacerbated following UVA. NLC formulated with Dynasan 118 were particularly susceptible to UVA exposure. The results indicate which could be the most suitable candidates for formulating NLC that are biocompatible and non-cytotoxic even when exposed to UVA and hence help direct future choices during the formulation strategies of these delivery systems. Of those tested, Compritol 888 ATO appears to be the best choice.
Cell Death and Disease, 2015
Journal of the Chemical Society, Perkin Transactions 2, 1994
... Liberato Cardellini,a Patricia Carloni,a Elisabetta Damiani,a Lucedio Greci,*Sa Pierluigi Sti... more ... Liberato Cardellini,a Patricia Carloni,a Elisabetta Damiani,a Lucedio Greci,*Sa Pierluigi Stipa,a Corrado Rizzoli and Paolo Sgarabotto *sb a ... compound 5' (Scheme I). This result was interpreted through the formation of the intermediate 2-phenyl-3-aryli-mino-3H-indoles 4 ...
Journal of the Chemical Society, Chemical Communications, 1993
ABSTRACT
Acta Chemica Scandinavica, 1998
... Acta Chemica Scandinavica ¡998 ACTA CHEMICA SCANDINAVICA ISSN 0904-213X Reactions of 1,2-Dihy... more ... Acta Chemica Scandinavica ¡998 ACTA CHEMICA SCANDINAVICA ISSN 0904-213X Reactions of 1,2-Dihydro-2-methyl-2-phenyl-3H-indole-3-one with Various Halogenating Reagents Patricia Carloni, Elisabetta ... Chem. Soc 85 (1963) 3139: Walling, С and Rieger, AL / Am. ...
Journal of the Chemical Society, Perkin Transactions 2, 2001
ABSTRACT
Clinical and Translational Allergy, 2013
Biochemical Society transactions, 1993
Journal of the Chemical Society, Perkin Transactions 2, 2000
Journal of the Chemical Society, Perkin Transactions 2, 1993
Five of the title N-oxides 1 when reduced either chemically (phenylhydrazine, hydrazobenzene) or ... more Five of the title N-oxides 1 when reduced either chemically (phenylhydrazine, hydrazobenzene) or electrochemically (DMF–TEAP–protonating agent) were found to afford the corresponding, 5-hydroxyindoles 6. The reduction process takes place in two steps ...
Research on Chemical Intermediates, 2000
Research on Chemical Intermediates, 1993
1,3-diphenylisobenzofuran (DPBF) is a fluorescent molecule which possesses a highly specific reac... more 1,3-diphenylisobenzofuran (DPBF) is a fluorescent molecule which possesses a highly specific reactivity towards singlet oxygen (1O2) forming an endoperoxide which decomposes to give 1,2-dibenzoylbenzene. This reaction between DPBF and 1O2 can be followed by measuring the decrease in fluorescence intensity of DPBF. In order to check the specificity of DPBF toward free radicals a series of experiments was carried out
Polymer Degradation and Stability, 1997
Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2003
In order to gain more knowledge on the antioxidant role of nitroxide radicals, in this study we i... more In order to gain more knowledge on the antioxidant role of nitroxide radicals, in this study we investigate their possible protective action against DNA damage induced by nitric oxide (NO) and reactive nitrogen oxide species deriving from it, namely nitroxyl anion (NO(-)) and peroxynitrite (ONOO(-)). Rat trachea epithelial cells were exposed under aerobic conditions to (1) NO generated by 150 microM S-nitrosoglutathione monoethyl ester (GSNO-MEE), (2) NO(-) generated by 200 microM Angeli's salt (Na(2)N(2)O(3)) (3) ONOO(-) generated by 1mM SIN-1 (3-morpholino-sydnonimine) and (4) 100 microM synthesized ONOO(-), in the absence and presence of 5 microM of two indolinonic nitroxides synthesized by us and the piperidine nitroxide TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl). DNA damage was assessed using the comet assay-a rapid and sensitive, single-cell gel electrophoresis technique used to detect primary DNA damage in individual cells. The parameter tail moment, used as an index of DNA damage, showed that in all cases the nitroxides remarkably inhibited DNA strand breaks induced by the different nitrogen oxide species. All three nitroxides protect to the same extent, except in the case of synthesized peroxynitrite where the aromatic nitroxides 1 and 2 are more efficient than TEMPO. These findings are consistent with the antioxidant character of nitroxide compounds and give additional information on the potential implications for their use as therapeutic agents.
International Journal of Pharmaceutics, 2012
Photostability of suncare products is a great area of interest since several sunscreens on the ma... more Photostability of suncare products is a great area of interest since several sunscreens on the market are photounstable, and this is primarily a problem concerning the UVA region (320-400 nm). Here we report a comparative study on the photostability assessment of two commercial sunscreens with same SPF, spread onto glass plates or onto full thickness pig ear skin or human/pig SCE membranes, and exposed to 183 kJ/m(2) UVA. Absorbance spectra and lipid peroxidation (measured by TBARS production) were determined. The results indicate: (a) sunscreen performance consequent to UVA exposure is independent of whether it is spread onto a non-biological and chemically inert substrate such as glass, or on biological substrates such as skin/SCE membranes; (b) despite the same SPF, sunscreen performance and photostability can be very different; (c) the data on human SCE membranes are similar to those on pig SCE membranes, indicating the suitability of the latter as a model for human skin. However, since the results obtained using skin membranes, akin to the more realistic conditions of use in vivo, do not substantially differ from those obtained on glass plates, the method proposed here using the latter may be applied for rapid, inexpensive, efficacy screening of photostability of sunscreens. Photostability testing should be a mandatory requirement for safer sunscreen protection products, since the results clearly show that some are still far from perfect.
International Journal of Pharmaceutics, 2015
Nanostructured lipid carriers (NLC) are widely used for topical delivery of active ingredients in... more Nanostructured lipid carriers (NLC) are widely used for topical delivery of active ingredients into the skin for both local and systemic treatment. But concerns have been raised regarding their potential nanotoxicity. To understand the role of NLC composition in terms of cytotoxicity and pro-oxidant effects, we investigated cell viability and intracellular levels of ROS (reactive oxygen species) production in human dermal fibroblasts (HDF) incubated with five NLC formulations differing in their solid lipid composition. HDF and NLC were also exposed to UVA irradiation in order to evaluate the behavior of NLC under realistic environmental conditions which might promote their instability. Using the Guava via-count assay, all nanoparticles, except for those formulated with Compritol 888 ATO, showed a significant decrease in live cells and a parallel increase in apoptotic or dead cells compared to the control, either before and/or after UVA irradiation (18J/cm(2)). NLC formulated with Geleol™ Mono Diglycerides resulted the most cytotoxic. A similar trend was also observed when intracellular ROS levels were measured in HDF incubated with NLC: there was increased ROS content compared to the control, further exacerbated following UVA. NLC formulated with Dynasan 118 were particularly susceptible to UVA exposure. The results indicate which could be the most suitable candidates for formulating NLC that are biocompatible and non-cytotoxic even when exposed to UVA and hence help direct future choices during the formulation strategies of these delivery systems. Of those tested, Compritol 888 ATO appears to be the best choice.
Cell Death and Disease, 2015
Journal of the Chemical Society, Perkin Transactions 2, 1994
... Liberato Cardellini,a Patricia Carloni,a Elisabetta Damiani,a Lucedio Greci,*Sa Pierluigi Sti... more ... Liberato Cardellini,a Patricia Carloni,a Elisabetta Damiani,a Lucedio Greci,*Sa Pierluigi Stipa,a Corrado Rizzoli and Paolo Sgarabotto *sb a ... compound 5' (Scheme I). This result was interpreted through the formation of the intermediate 2-phenyl-3-aryli-mino-3H-indoles 4 ...
Journal of the Chemical Society, Chemical Communications, 1993
ABSTRACT
Acta Chemica Scandinavica, 1998
... Acta Chemica Scandinavica ¡998 ACTA CHEMICA SCANDINAVICA ISSN 0904-213X Reactions of 1,2-Dihy... more ... Acta Chemica Scandinavica ¡998 ACTA CHEMICA SCANDINAVICA ISSN 0904-213X Reactions of 1,2-Dihydro-2-methyl-2-phenyl-3H-indole-3-one with Various Halogenating Reagents Patricia Carloni, Elisabetta ... Chem. Soc 85 (1963) 3139: Walling, С and Rieger, AL / Am. ...
Journal of the Chemical Society, Perkin Transactions 2, 2001
ABSTRACT
Clinical and Translational Allergy, 2013
Biochemical Society transactions, 1993
Journal of the Chemical Society, Perkin Transactions 2, 2000
Journal of the Chemical Society, Perkin Transactions 2, 1993
Five of the title N-oxides 1 when reduced either chemically (phenylhydrazine, hydrazobenzene) or ... more Five of the title N-oxides 1 when reduced either chemically (phenylhydrazine, hydrazobenzene) or electrochemically (DMF–TEAP–protonating agent) were found to afford the corresponding, 5-hydroxyindoles 6. The reduction process takes place in two steps ...
Research on Chemical Intermediates, 2000
Research on Chemical Intermediates, 1993
1,3-diphenylisobenzofuran (DPBF) is a fluorescent molecule which possesses a highly specific reac... more 1,3-diphenylisobenzofuran (DPBF) is a fluorescent molecule which possesses a highly specific reactivity towards singlet oxygen (1O2) forming an endoperoxide which decomposes to give 1,2-dibenzoylbenzene. This reaction between DPBF and 1O2 can be followed by measuring the decrease in fluorescence intensity of DPBF. In order to check the specificity of DPBF toward free radicals a series of experiments was carried out
Polymer Degradation and Stability, 1997
Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2003
In order to gain more knowledge on the antioxidant role of nitroxide radicals, in this study we i... more In order to gain more knowledge on the antioxidant role of nitroxide radicals, in this study we investigate their possible protective action against DNA damage induced by nitric oxide (NO) and reactive nitrogen oxide species deriving from it, namely nitroxyl anion (NO(-)) and peroxynitrite (ONOO(-)). Rat trachea epithelial cells were exposed under aerobic conditions to (1) NO generated by 150 microM S-nitrosoglutathione monoethyl ester (GSNO-MEE), (2) NO(-) generated by 200 microM Angeli's salt (Na(2)N(2)O(3)) (3) ONOO(-) generated by 1mM SIN-1 (3-morpholino-sydnonimine) and (4) 100 microM synthesized ONOO(-), in the absence and presence of 5 microM of two indolinonic nitroxides synthesized by us and the piperidine nitroxide TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl). DNA damage was assessed using the comet assay-a rapid and sensitive, single-cell gel electrophoresis technique used to detect primary DNA damage in individual cells. The parameter tail moment, used as an index of DNA damage, showed that in all cases the nitroxides remarkably inhibited DNA strand breaks induced by the different nitrogen oxide species. All three nitroxides protect to the same extent, except in the case of synthesized peroxynitrite where the aromatic nitroxides 1 and 2 are more efficient than TEMPO. These findings are consistent with the antioxidant character of nitroxide compounds and give additional information on the potential implications for their use as therapeutic agents.