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Papers by Damir Lukačević

Results of serological testing on canine leishmaniosis (indirect immunofluorescence) conducted du... more Results of serological testing on canine leishmaniosis (indirect immunofluorescence) conducted during the first nine months in 2008 were shown. Among group of dogs suspected to have leishmaniosis, 40.4% were seropositive in enzootic region, while in non-enzootic region seropositive were 28.9 %. This indicates primarily that the tanja.zivicnjak@gmail.com Živičnjak i sur.,SEROLOŠKI MONITORING LIŠMANIOZE PASA U HRVATSKOJ veterinarians have been well skilled in diagnostics. On the other hand, in the enzootic region 14,2% asymptomatic dogs were seropositive too. That indicates that monitoring among the whole canine population in enzootic area should be necessary in order to control this zoonosis.

In Mediterranean country Croatia, canine leishmaniasis has been recognized in the south littoral ... more In Mediterranean country Croatia, canine leishmaniasis has been recognized in the south littoral parts (Dalmatia region) as the re-emerging disease since 1997. Previous entomological surveys carried out in the area, revealed sand fly species of Phlebotomus genus, among which three were belonging to the Larroussius subgenus, being proven Leishmania infantum vectors. Because of the epizootiological pattern of leishmaniasis in Dalmatia, obligatory testing of all dogs exhibiting evident clinical symptoms of leishmaniasis had been decreed by Croatian Ministry of Agriculture, Forestry and Water Management during 2002-2005 period. During this period, efforts on serological monitoring of the asymptomatic canine population in enzootic area had been implemented. The incidence rate of seropositive dogs had decreased during this period from 15% to 8%. In 2005 measures had been perceived by Ministry as too expensive, and in 2006 ultimately abandoned. Since then, we have been witnessing doubts, w...

Anaplazme su alfa-proteobakterije koje se prenose razlicitim vrstama krpelja i prosirene su po cj... more Anaplazme su alfa-proteobakterije koje se prenose razlicitim vrstama krpelja i prosirene su po cjelom svijetu. Molekularnim istraživanjima dokazani su brojni izolati, a danas priznate vrste su Anaplasma phagocytophilum, A. bovis, A. ovis, A. platys, A. centrale i novo opisana vrsta A. odocoieili 2013. godine. Premda se i dalje smatraju "tropskim" uzrocnicima bolesti, prisutne su u Europi stoga je cilj ovog rada prikazati trenutacne spoznaje o anaplazmama u domacih i divljih životinja te kucnih ljubimaca u Republici Hrvatskoj. Pretraženi su serumi 1215 pasa SNAP 4Dx testom (IDEXX), a DNAizdvojena iz krvi 1080 pasa, 15 ovaca kao i 834 organa divljih je pretražena je lancanom reakcijom polimerazom sa neposrednim sekvencioniranjem umnoženih odsjecaka. Molekularnim pretragama dokazane su vrste Anaplasma phagocytophilum, A. bovis, A. ovis, A. platys i A. centrale dok je seroloski bilo 5% pasa pozitivno.

Svrha ovog istraživanja bila je molekularnim i seroloskim metodama pretražiti uzorke krvi naizgle... more Svrha ovog istraživanja bila je molekularnim i seroloskim metodama pretražiti uzorke krvi naizgled zdravih pasa koji žive u poznatim žaristima enzootskog podrucja, izvan tih žarista i u granicnim podrucjima, te dobiti uvid u stupanj rasirenosti lismanioze. Cilj je također bio istražiti mogucnosti kombiniranja dijagnostickih metoda razlicitih osjetljivosti i specificnosti te standardizirati protokol koji ce omoguciti implementaciju kvalitetnog pracenja lismanioze pasa, kako u enzootskom podrucju, tako i na podrucjima gdje se bolest dosad nije pojavljivala. Pretraživanjem na podrucju tri dalmatinske županije obuhvacena su ukupno 242 naizgled zdrava psa, raznih pasmina i kategorija, od cega su najzastupljeniji bili lovacki. Svi uzorci su testirani primjenom cetiri seroloske i tri molekularne metode, te su rezultati uspoređeni i statisticki obrađeni. Prvom primjenom multiplog simultanog testiranja u enzootskom podrucju, postigli smo otkrivanje subklinicki invadiranih pasa, ali i elimina...

Parasitology Research, 2018

Canine vector-borne pathogens (VBPs) are a group of globally distributed and rapidly spreading mi... more Canine vector-borne pathogens (VBPs) are a group of globally distributed and rapidly spreading microorganisms transmitted by arthropods. In the present survey, we investigated Anaplasma spp., Ehrlichia canis, Dirofilaria immitis and Borrelia burgdorferi sensu lato seroprevalence between three groups of dogs (asymptomatic, suspected and deceased) from continental and coastal regions of Croatia with the commercial point-of-care SNAP®4Dx®Plus for VBPs. The overall prevalence for tested pathogens in dogs was 6.1% with the highest prevalence detected for Anaplasma spp. (4.5%), while the remaining pathogens were found at a prevalence of less than 1% (E. canis 0.6%, B. burgdorferi s.l. 0.4%, D. immitis 0.6%). No statistically significant differences in VBP detection between dog cohorts could be found with the exception of D. immitis in the deceased group. Interestingly, no evidence of D. immitis could be found in the hearts of dogs in this group at necropsy, however. This study provides the first data on the seroprevalence of selected VBPs between dogs of different health statuses in Croatia. The results demonstrate that serological evidence of VBPs alone or in combination with co-infections were found just as frequently in asymptomatic dogs as those with suspected or confirmed evidence of VBP disease, raising questions about the pathogenic potential of these organisms in domesticated dogs.

Parasitology Research, 2017

In Croatia, canine leishmaniosis has been recognized in the southern littoral area (Dalmatia) as ... more In Croatia, canine leishmaniosis has been recognized in the southern littoral area (Dalmatia) as a re-In Croatia, canine leishmaniosis has been recognized in the southern littoral area (Dalmatia) as a re-emerging disease since 1997. This paper reports the fi ndings of a survey carried out 2.5 years after the suspension emerging disease since 1997. This paper reports the fi ndings of a survey carried out 2.5 years after the suspension of control measures for canine leishmaniosis. Entomological and cross-sectional serological surveys were of control measures for canine leishmaniosis. Entomological and cross-sectional serological surveys were carried out in the Kaštela municipality in the Split-Dalmatia County (where stable foci of canine leishmaniosis carried out in the Kaštela municipality in the Split-Dalmatia County (where stable foci of canine leishmaniosis were reported previously), and in a few localities from the Šibenik-Knin county where a few unstable foci were were reported ...

Journal of Veterinary Medicine Series B, 2006

The aim of the study was to give an account of the epidemic of abortions in sheep caused by Salmo... more The aim of the study was to give an account of the epidemic of abortions in sheep caused by Salmonella enterica ssp. enterica serovar Abortusovis, which occurred in Dalmatia, south Croatia, in winter 2003-2004. Five sheep flocks with rate of abortion ranging from 22% to 38% during the last-third of gestation were examined. Salmonella Abortusovis was isolated from 13 vaginal smears and two fetuses. Direct inoculation was found to be superior to pre-enrichment and enrichment in selective broth for Salmonella Abortusovis isolation. The isolates were biochemically identified, and characterized by serotyping and polymerase chain reaction based on the amplification of the IS200 sequence specific for Salmonella Abortusovis. A fragment of 900 bp was detected in all Salmonella Abortusovis isolates. The sensitivity testing of the isolates, carried out by the disk diffusion method and the determination of the minimal inhibitory concentrations, resulted in a high sensitivity to almost all antimicrobials used. Only two isolates were moderately sensitive to oxytetracycline, whereas one isolate showed resistance to streptomycin. Campylobacter fetus ssp. fetus and Listeria monocytogenes were excluded as causative agents of abortion in sheep by culture testing, and brucellosis, leptospirosis, Q fever and chlamydiosis by serological testing.

U radu je opisana epizootija giardioze kao uzroka pojave proljeva u mlade teladi. Tijekom trajanj... more U radu je opisana epizootija giardioze kao uzroka pojave proljeva u mlade teladi. Tijekom trajanja bolesti, parazitološkim i bakteriološkim pretragama pretraženi su uzorci izmeta 21 goveda. Uzročnik je dokazan u izmetu klinički bolesnog teleta te u izmetu 4 odrasla klinički zdrava goveda. Brzo ozdravljenje nakon primjene albendazola potvrđuje nametničku osnovu bolesti. Klinički očitovana giardioza u teladi i supklinička invadiranost odraslih goveda očito su prisutne na području Dalmacije, ali zasigurno nisu dovoljno prepoznate. Cilj je ovoga rada skrenuti pozornost veterinara praktičara na ovu parazitozu kako bi pravodobnim prepoznavanjem i adekvatnom terapijom umanjili štete nastale zbog bolesti i uginuća te posredno sprečavali kontaminacije pašnjaka i nastambi cistama uzročnika.The paper describes epizootic giardiosis as the cause of diarrhoea in young calves. During the time of the disease outbreak 21 cattle stool samples were examined, using parasitological and bacteriological t...

Results of serological testing on canine leishmaniosis (indirect immunofluorescence) conducted du... more Results of serological testing on canine leishmaniosis (indirect immunofluorescence) conducted during the first nine months in 2008 were shown. Among group of dogs suspected to have leishmaniosis, 40.4% were seropositive in enzootic region, while in non-enzootic region seropositive were 28.9 %. This indicates primarily that the tanja.zivicnjak@gmail.com Živičnjak i sur.,SEROLOŠKI MONITORING LIŠMANIOZE PASA U HRVATSKOJ veterinarians have been well skilled in diagnostics. On the other hand, in the enzootic region 14,2% asymptomatic dogs were seropositive too. That indicates that monitoring among the whole canine population in enzootic area should be necessary in order to control this zoonosis.

In Mediterranean country Croatia, canine leishmaniasis has been recognized in the south littoral ... more In Mediterranean country Croatia, canine leishmaniasis has been recognized in the south littoral parts (Dalmatia region) as the re-emerging disease since 1997. Previous entomological surveys carried out in the area, revealed sand fly species of Phlebotomus genus, among which three were belonging to the Larroussius subgenus, being proven Leishmania infantum vectors. Because of the epizootiological pattern of leishmaniasis in Dalmatia, obligatory testing of all dogs exhibiting evident clinical symptoms of leishmaniasis had been decreed by Croatian Ministry of Agriculture, Forestry and Water Management during 2002-2005 period. During this period, efforts on serological monitoring of the asymptomatic canine population in enzootic area had been implemented. The incidence rate of seropositive dogs had decreased during this period from 15% to 8%. In 2005 measures had been perceived by Ministry as too expensive, and in 2006 ultimately abandoned. Since then, we have been witnessing doubts, w...

Anaplazme su alfa-proteobakterije koje se prenose razlicitim vrstama krpelja i prosirene su po cj... more Anaplazme su alfa-proteobakterije koje se prenose razlicitim vrstama krpelja i prosirene su po cjelom svijetu. Molekularnim istraživanjima dokazani su brojni izolati, a danas priznate vrste su Anaplasma phagocytophilum, A. bovis, A. ovis, A. platys, A. centrale i novo opisana vrsta A. odocoieili 2013. godine. Premda se i dalje smatraju "tropskim" uzrocnicima bolesti, prisutne su u Europi stoga je cilj ovog rada prikazati trenutacne spoznaje o anaplazmama u domacih i divljih životinja te kucnih ljubimaca u Republici Hrvatskoj. Pretraženi su serumi 1215 pasa SNAP 4Dx testom (IDEXX), a DNAizdvojena iz krvi 1080 pasa, 15 ovaca kao i 834 organa divljih je pretražena je lancanom reakcijom polimerazom sa neposrednim sekvencioniranjem umnoženih odsjecaka. Molekularnim pretragama dokazane su vrste Anaplasma phagocytophilum, A. bovis, A. ovis, A. platys i A. centrale dok je seroloski bilo 5% pasa pozitivno.

Svrha ovog istraživanja bila je molekularnim i seroloskim metodama pretražiti uzorke krvi naizgle... more Svrha ovog istraživanja bila je molekularnim i seroloskim metodama pretražiti uzorke krvi naizgled zdravih pasa koji žive u poznatim žaristima enzootskog podrucja, izvan tih žarista i u granicnim podrucjima, te dobiti uvid u stupanj rasirenosti lismanioze. Cilj je također bio istražiti mogucnosti kombiniranja dijagnostickih metoda razlicitih osjetljivosti i specificnosti te standardizirati protokol koji ce omoguciti implementaciju kvalitetnog pracenja lismanioze pasa, kako u enzootskom podrucju, tako i na podrucjima gdje se bolest dosad nije pojavljivala. Pretraživanjem na podrucju tri dalmatinske županije obuhvacena su ukupno 242 naizgled zdrava psa, raznih pasmina i kategorija, od cega su najzastupljeniji bili lovacki. Svi uzorci su testirani primjenom cetiri seroloske i tri molekularne metode, te su rezultati uspoređeni i statisticki obrađeni. Prvom primjenom multiplog simultanog testiranja u enzootskom podrucju, postigli smo otkrivanje subklinicki invadiranih pasa, ali i elimina...

Parasitology Research, 2018

Canine vector-borne pathogens (VBPs) are a group of globally distributed and rapidly spreading mi... more Canine vector-borne pathogens (VBPs) are a group of globally distributed and rapidly spreading microorganisms transmitted by arthropods. In the present survey, we investigated Anaplasma spp., Ehrlichia canis, Dirofilaria immitis and Borrelia burgdorferi sensu lato seroprevalence between three groups of dogs (asymptomatic, suspected and deceased) from continental and coastal regions of Croatia with the commercial point-of-care SNAP®4Dx®Plus for VBPs. The overall prevalence for tested pathogens in dogs was 6.1% with the highest prevalence detected for Anaplasma spp. (4.5%), while the remaining pathogens were found at a prevalence of less than 1% (E. canis 0.6%, B. burgdorferi s.l. 0.4%, D. immitis 0.6%). No statistically significant differences in VBP detection between dog cohorts could be found with the exception of D. immitis in the deceased group. Interestingly, no evidence of D. immitis could be found in the hearts of dogs in this group at necropsy, however. This study provides the first data on the seroprevalence of selected VBPs between dogs of different health statuses in Croatia. The results demonstrate that serological evidence of VBPs alone or in combination with co-infections were found just as frequently in asymptomatic dogs as those with suspected or confirmed evidence of VBP disease, raising questions about the pathogenic potential of these organisms in domesticated dogs.

Parasitology Research, 2017

In Croatia, canine leishmaniosis has been recognized in the southern littoral area (Dalmatia) as ... more In Croatia, canine leishmaniosis has been recognized in the southern littoral area (Dalmatia) as a re-In Croatia, canine leishmaniosis has been recognized in the southern littoral area (Dalmatia) as a re-emerging disease since 1997. This paper reports the fi ndings of a survey carried out 2.5 years after the suspension emerging disease since 1997. This paper reports the fi ndings of a survey carried out 2.5 years after the suspension of control measures for canine leishmaniosis. Entomological and cross-sectional serological surveys were of control measures for canine leishmaniosis. Entomological and cross-sectional serological surveys were carried out in the Kaštela municipality in the Split-Dalmatia County (where stable foci of canine leishmaniosis carried out in the Kaštela municipality in the Split-Dalmatia County (where stable foci of canine leishmaniosis were reported previously), and in a few localities from the Šibenik-Knin county where a few unstable foci were were reported ...

Journal of Veterinary Medicine Series B, 2006

The aim of the study was to give an account of the epidemic of abortions in sheep caused by Salmo... more The aim of the study was to give an account of the epidemic of abortions in sheep caused by Salmonella enterica ssp. enterica serovar Abortusovis, which occurred in Dalmatia, south Croatia, in winter 2003-2004. Five sheep flocks with rate of abortion ranging from 22% to 38% during the last-third of gestation were examined. Salmonella Abortusovis was isolated from 13 vaginal smears and two fetuses. Direct inoculation was found to be superior to pre-enrichment and enrichment in selective broth for Salmonella Abortusovis isolation. The isolates were biochemically identified, and characterized by serotyping and polymerase chain reaction based on the amplification of the IS200 sequence specific for Salmonella Abortusovis. A fragment of 900 bp was detected in all Salmonella Abortusovis isolates. The sensitivity testing of the isolates, carried out by the disk diffusion method and the determination of the minimal inhibitory concentrations, resulted in a high sensitivity to almost all antimicrobials used. Only two isolates were moderately sensitive to oxytetracycline, whereas one isolate showed resistance to streptomycin. Campylobacter fetus ssp. fetus and Listeria monocytogenes were excluded as causative agents of abortion in sheep by culture testing, and brucellosis, leptospirosis, Q fever and chlamydiosis by serological testing.

U radu je opisana epizootija giardioze kao uzroka pojave proljeva u mlade teladi. Tijekom trajanj... more U radu je opisana epizootija giardioze kao uzroka pojave proljeva u mlade teladi. Tijekom trajanja bolesti, parazitološkim i bakteriološkim pretragama pretraženi su uzorci izmeta 21 goveda. Uzročnik je dokazan u izmetu klinički bolesnog teleta te u izmetu 4 odrasla klinički zdrava goveda. Brzo ozdravljenje nakon primjene albendazola potvrđuje nametničku osnovu bolesti. Klinički očitovana giardioza u teladi i supklinička invadiranost odraslih goveda očito su prisutne na području Dalmacije, ali zasigurno nisu dovoljno prepoznate. Cilj je ovoga rada skrenuti pozornost veterinara praktičara na ovu parazitozu kako bi pravodobnim prepoznavanjem i adekvatnom terapijom umanjili štete nastale zbog bolesti i uginuća te posredno sprečavali kontaminacije pašnjaka i nastambi cistama uzročnika.The paper describes epizootic giardiosis as the cause of diarrhoea in young calves. During the time of the disease outbreak 21 cattle stool samples were examined, using parasitological and bacteriological t...