Rosemeri Dams - Academia.edu (original) (raw)
Papers by Rosemeri Dams
BioEnergy Research, 2019
Crude glycerol has become an attractive feedstock for biohydrogen production via dark fermentatio... more Crude glycerol has become an attractive feedstock for biohydrogen production via dark fermentation. However, it is necessary to determine the inoculation conditions that provide the highest biohydrogen yield. The aim of this work was to evaluate in batch assays the effect of the inoculum and methanogenesis inhibition method on biohydrogen production from crude glycerol. Four inocula were tested: anaerobic sludge treating municipal wastewater, anaerobic sludge treating brewery wastewater, goat ruminal liquid, and a mixture of the first three inocula. Each inoculum was subjected to three different treatments to inhibit methanogenesis: addition of chloroform, acid shock, and heat shock. Denaturing gradient gel electrophoresis (DGGE) of the polymerase chain reaction (PCR) products was used to assess microbial communities present in the pretreated inocula. The results indicate that the ruminal liquid treated with chloroform produced the highest biohydrogen yield (0.208 mol H 2 mol −1 glycerol). The microbial community present after all treatments tested preserved good functionality and stability in terms of species composition, and could endure changing environmental conditions.
Caproic acid (hexanoic acid), which is a six-carbon saturated fatty acid, currently is considered... more Caproic acid (hexanoic acid), which is a six-carbon saturated fatty acid, currently is considered a valuable industrial commodity and has twice the value of ethanol per carbon atom. It can be produced by chain elongation from ethanol and acetic acid and it has been used in several industrial applications such as the food industry, medicine, and chemical industry. The chain elongation process with ethanol was used to evaluate the production of caproic acid in anaerobic granular and flocculent sludge, as well as goat ruminal liquid under different culturing conditions. Three different concentrations of substrate, namely 100/25, 200/50, and 400/100 mM of ethanol and mM acetic acid, respectively, were tested at two different pH levels of 5.5 and 7.0. A ncaproic acid concentration of ~ 3 g/L was obtained with the granular sludge as an inoculum at a pH of 5.5; however, the highest concentrations of ~ 4.5 g/L and 4.9 g/L was obtained with granular sludge and ruminal liquid at a pH 7.0, res...
Boletim do Centro de Pesquisa …, 2009
Resumo Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de ... more Resumo Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de bactérias patógenas e de microrganismos indicadores de contaminação, em amostras colhidas na forma inteira e em filés,in natura e congeladas. Em 80% das ...
Engenharia Sanitaria E Ambiental, Dec 1, 2007
O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenol... more O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenolicaem solo argiloso na presença e ausência de trigo. As concentrações de PCP foram determinadas através de Análises de Alta Performance de Cromatografia Líquida. Os efeitos tóxicos de PCP foram estudados através do monitoramento do crescimento das plantas. A biodegradação de PCP por S. chlorophenolica foi acompanhada por testes de bioluminescência de Escherichia coli HB101 pUCD607 e contagens bacterianas no solo e nas raízes. A degradação de PCP ocorreu de forma mais rápida no solo plantado e inoculado quando comparada ao solo sem plantas. Houve um aumento significativo nas populações dos organismos testados nas raízes quando comparadas com as populações presentes no solo. O monitoramento do crescimento da planta mostrou o papel protetor exercido pela S.chlorophenolica contra a toxicidade do PCP.
Environmental Technology
Chlorophenols are well-known priority pollutants and many different treatments have been assessed... more Chlorophenols are well-known priority pollutants and many different treatments have been assessed to facilitate their removal from industrial wastewater. However, an absolute and optimum solution still has to be practically implemented in an industrial setting. In this work, a series ofphysical, chemical and biochemical treatments have been systematically tested for the removal of 4-chlorophenol, and their results have been compared in order to determine the most effective treatment based on removal efficiency and residual by-product formation. Chemical treatments based on advanced oxidation processes (AOP) produced the best results on rate and extent of pollutant removal. The non-chemical technologies showed advantages in terms of complete (in the case of adsorption) or easy (enzymatic treatments) removal of toxic treatment by-products. The AOP methods led to the production of different photoproducts depending on the chosen treatment. Toxic products remained in most cases following...
Environmental technology, Jan 26, 2016
The aim of this research was to estimate the production of hydrogen, organic acids and alcohols b... more The aim of this research was to estimate the production of hydrogen, organic acids and alcohols by the strain of Clostridium acetobutylicum ATCC 824 using residual glycerol as a carbon source. The experiments were carried out in pure and mixed cultures in batch experiments. Three different sources of inocula for mixed culture were used. Ruminal liquid from goats and sludge collected from two upflow anaerobic sludge blanket reactors treating municipal wastewater and brewery effluent were tested for hydrogen, organic acids and alcohols production with or without C. acetobutylicum ATCC 824. The main detected end-products from the glycerol fermentation were hydrogen, organic acids (acetic, propionic, butyric and caproic) and alcohol (ethanol and 1,3-propanediol - 1,3PD). High hydrogen (0.44 mol H2/mol glycerol consumed) and 1,3PD (0.32 mol 1,3PD/mol glycerol consumed) yields were obtained when the strain C. acetobutylicum ATCC 824 was bioaugmented into the sludge from municipal wastewat...
Boletim do Centro de Pesquisa de Processamento de Alimentos, 2009
Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de bactéri... more Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de bactérias patógenas e de microrganismos indicadores de contaminação, em amostras colhidas na forma inteira e em filés, “in natura” e congeladas. Em 80% das amostras de filé e 40% do pescado inteiro “in natura”, constatou-se Salmonella spp, situando-se a contagem padrão de psicrotróficos entre limites aceitáveis pelo ICMSF. Não se constatou presença de bactérias coliformes fecais, apenas coliformes totais e número não significativo de Staphylococcus aureus. Os resultados indicam deficiência de higiêne e sanificação nas operações rotineiras do processamento.
Engenharia Sanitaria e Ambiental, 2007
O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenol... more O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenolicaem solo argiloso na presença e ausência de trigo. As concentrações de PCP foram determinadas através de Análises de Alta Performance de Cromatografia Líquida. Os efeitos tóxicos de PCP foram estudados através do monitoramento do crescimento das plantas. A biodegradação de PCP por S. chlorophenolica foi acompanhada por testes de bioluminescência de Escherichia coli HB101 pUCD607 e contagens bacterianas no solo e nas raízes. A degradação de PCP ocorreu de forma mais rápida no solo plantado e inoculado quando comparada ao solo sem plantas. Houve um aumento significativo nas populações dos organismos testados nas raízes quando comparadas com as populações presentes no solo. O monitoramento do crescimento da planta mostrou o papel protetor exercido pela S.chlorophenolica contra a toxicidade do PCP.
Boletim do Centro de Pesquisa de Processamento de Alimentos, 2009
Apresenta revisão bibliográfica sobre o Sistema de Análise de Risco e Pontos Críticos de Controle... more Apresenta revisão bibliográfica sobre o Sistema de Análise de Risco e Pontos Críticos de Controle. Comenta a origem do sistema e a sua importância na garantia da qualidade e segurança do produto alimentício. São discutidas as suas aplicações na indústria de alimentos, mostrando seus objetivos e elementos, além de enfatizar os procedimentos necessários para sua implantação.
Boletim do Centro de Pesquisa de Processamento de Alimentos, 2009
Elaborou-se Manual de Boas Práticas de Higiene e Sanificação para Indústria de Pescado Congelado ... more Elaborou-se Manual de Boas Práticas de Higiene e Sanificação para Indústria de Pescado Congelado com o objetivo de orientar, de forma simples e prática os manipuladores e todos os envolvidos no processamento do pescado, quanto às práticas de higiene e de sanificação necessárias para a obtenção de produto de qualidade. Para tanto montou-se fluxograma do processamento de congelamento da indústria de pescado congelado, identificando-se os principais pontos críticos de controle. Levantou-se as condições higiênico-sanitárias dos equipamentos, utensílios e processamento, assim como os hábitos higiênicos dos manipuladores. Acredita-se que o uso do Manual possa propiciar sanificação e condições adequadas de higiene para o trabalho, garantindo a qualidade do produtos.
Pesticidas: Revista de Ecotoxicologia e Meio Ambiente, 2007
O objetivo deste trabalho foi estudar a degradação de pentaclorophenol (PCP) por S. chlorophenoli... more O objetivo deste trabalho foi estudar a degradação de pentaclorophenol (PCP) por S. chlorophenolica em dois diferentes tipos de solo (arenoso e argiloso) na presença e ausência de plantas (trigo - Triticum aestivum). As concentrações de PCP foram determinadas mediante Cromatografia a Líquido de Alta Eficiência (CLAE). Os efeitos tóxicos de PCP foram estudados pelo monitoramento do crescimento das plantas (em peso, g) e medidas das raízes (cm). A biodegradação de PCP por S. chlorophenolica nos dois tipos de solo foi acompanhada por análises de bioluminescência de Escherichia coli HB101 pUCD607. Contagens bacterianas foram realizadas em três meios de cultura: meio mineral para S. chlorophenolica, meio mineral para organismos degradadores/tolerantes ao PCP e ágar triptose caldo de soja para organismos heterotróficos. No solo argiloso com vegetação, a degradação de PCP ocorreu de forma mais rápida após a introdução de S. chlorophenolica que no solo sem plantas. O monitoramento do cresc...
Separation Science and Technology, 2010
4-Chlorophenol removal combining KrCl excimer UV lamp and H2O2 has been studied, using 4-chloroph... more 4-Chlorophenol removal combining KrCl excimer UV lamp and H2O2 has been studied, using 4-chlorophenol concentrations of 100 and 250 mg L and molar ratios H2O2: 4-chlorophenol = 1:1, 10:1, 25:1, and 50:1. A ratio of 25:1 achieves the total removal for both 4-chlorophenol and the photoproducts. Comparing these results with those corresponding to the use of excilamp treatment in the absence of H2O2, there
Journal of Hazardous Materials, 2011
The effect of silver ions, nano-and micro-particles on a luminescent biosensor bacterium Pseudomo... more The effect of silver ions, nano-and micro-particles on a luminescent biosensor bacterium Pseudomonas putida originally isolated from activated sludge was assessed. The bacterium carrying a stable chromosomal copy of the lux operon (luxCDABE) was able to detect toxicity of ionic and particulate silver over short term incubations ranging from 30 to 240 min. The IC 50 values obtained at different time intervals showed that highest toxicity (lowest IC 50) was obtained after 90 min incubation for all toxicants and this is considered the optimum incubation for testing. The data show that ionic silver is the most toxic followed by nanosilver particles with microsilver particles being least toxic. Release of nanomaterials is likely to have an effect on the activated sludge process as indicated by the study using a common sludge bacterium involved in biodegradation of organic wastes.
Journal of Hazardous Materials, 2009
Time-course performance of a phenol-degrading indigenous bacterial consortium, and of Acinetobact... more Time-course performance of a phenol-degrading indigenous bacterial consortium, and of Acinetobacter calcoaceticus var. anitratus, isolated from an industrial coal wastewater treatment plant was evaluated. This bacterial consortium was able to survive in the presence of phenol concentrations as high as 1200 mg L −1 and the consortium was more fast in degrading phenol than a pure culture of the A. calcoaceticus strain. In a batch system, 86% of phenol biodegradation occurred in around 30 h at pH 6.0, while at pH 3.0, 95.2% of phenol biodegradation occurred in 8 h. A high phenol biodegradation (above 95%) by the mixed culture in a bioreactor was obtained in both continuous and batch systems, but when test was carried out in coke gasification wastewater, no biodegradation was observed after 10 days at pH 9-11 for both pure strain or the isolated consortium. An activated sludge with the same bacterial consortium characterized above was mixed with a textile sludge-contaminated soil with a phenol concentration of 19.48 mg kg −1. After 20 days of bioaugmentation, the remanescent phenol concentration of the sludge-soil matrix was 1.13 mg kg −1 .
International Biodeterioration & Biodegradation, 2007
Sphingobium chlorophenolicum is well known as pentachlorophenol (PCP) degrader. The objective of ... more Sphingobium chlorophenolicum is well known as pentachlorophenol (PCP) degrader. The objective of this study was to evaluate the PCP degradation in soil and hydroponic systems using a bioaugmentation technique. Measurements of PCP concentrations were carried out using high-performance liquid chromatography (HPLC) analyses. The toxic effect of PCP on plants was studied through the monitoring of plant weight. An acute toxicity
Environmental Technology, 2012
This article may be used for research, teaching, and private study purposes. Any substantial or s... more This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.
Chemosphere, 2007
Sphingobium chlorophenolicum is well known as a pentachlorophenol (PCP) degrader. The objective o... more Sphingobium chlorophenolicum is well known as a pentachlorophenol (PCP) degrader. The objective of this study was to evaluate PCP degradation in a loamy sandy soil artificially contaminated with PCP using phytoremediation and bioaugmentation. Measurements of PCP concentrations were carried out using high performance liquid chromatography analyses (HPLC). The toxic effect of PCP on plants was studied through the monitoring of weight plant and root length. The biodegradation of PCP by S. chlorophenolicum in soil was assessed with a bioluminescence assay of Escherichia coli HB101 pUCD607. Bacterial analyses were carried out by plating on Mineral Salt Medium (MSM) for S. chlorophenolicum, MSM for PCP-degrading/tolerant organisms and Trypticase Soy Broth Agar (TSBA) for heterotrophic organisms. The introduction of S. chlorophenolicum into soil with plants showed a faster degradation when compared to the non-inoculated soil. The monitoring of the plant growth showed a protective role of S. chlorophenolicum against the toxicity of PCP. The bioassay confirmed that initial toxicity was lowered while degradation progressed. There was a significant increase of organisms tested in the roots in comparison to those in the soil. This study showed that the presence of S. chlorophenolicum enhanced the PCP degradation in a loamy soil and also it had a protective role to prevent phytotoxic effects of PCP on plant growth. The combined use of bioaugmentation and plants suggests that the rhizosphere of certain plant species may be important for facilitating microbial degradation of pesticides in soil with important implications for using vegetation to stabilize and remediate surface soils.
BioEnergy Research, 2019
Crude glycerol has become an attractive feedstock for biohydrogen production via dark fermentatio... more Crude glycerol has become an attractive feedstock for biohydrogen production via dark fermentation. However, it is necessary to determine the inoculation conditions that provide the highest biohydrogen yield. The aim of this work was to evaluate in batch assays the effect of the inoculum and methanogenesis inhibition method on biohydrogen production from crude glycerol. Four inocula were tested: anaerobic sludge treating municipal wastewater, anaerobic sludge treating brewery wastewater, goat ruminal liquid, and a mixture of the first three inocula. Each inoculum was subjected to three different treatments to inhibit methanogenesis: addition of chloroform, acid shock, and heat shock. Denaturing gradient gel electrophoresis (DGGE) of the polymerase chain reaction (PCR) products was used to assess microbial communities present in the pretreated inocula. The results indicate that the ruminal liquid treated with chloroform produced the highest biohydrogen yield (0.208 mol H 2 mol −1 glycerol). The microbial community present after all treatments tested preserved good functionality and stability in terms of species composition, and could endure changing environmental conditions.
Caproic acid (hexanoic acid), which is a six-carbon saturated fatty acid, currently is considered... more Caproic acid (hexanoic acid), which is a six-carbon saturated fatty acid, currently is considered a valuable industrial commodity and has twice the value of ethanol per carbon atom. It can be produced by chain elongation from ethanol and acetic acid and it has been used in several industrial applications such as the food industry, medicine, and chemical industry. The chain elongation process with ethanol was used to evaluate the production of caproic acid in anaerobic granular and flocculent sludge, as well as goat ruminal liquid under different culturing conditions. Three different concentrations of substrate, namely 100/25, 200/50, and 400/100 mM of ethanol and mM acetic acid, respectively, were tested at two different pH levels of 5.5 and 7.0. A ncaproic acid concentration of ~ 3 g/L was obtained with the granular sludge as an inoculum at a pH of 5.5; however, the highest concentrations of ~ 4.5 g/L and 4.9 g/L was obtained with granular sludge and ruminal liquid at a pH 7.0, res...
Boletim do Centro de Pesquisa …, 2009
Resumo Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de ... more Resumo Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de bactérias patógenas e de microrganismos indicadores de contaminação, em amostras colhidas na forma inteira e em filés,in natura e congeladas. Em 80% das ...
Engenharia Sanitaria E Ambiental, Dec 1, 2007
O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenol... more O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenolicaem solo argiloso na presença e ausência de trigo. As concentrações de PCP foram determinadas através de Análises de Alta Performance de Cromatografia Líquida. Os efeitos tóxicos de PCP foram estudados através do monitoramento do crescimento das plantas. A biodegradação de PCP por S. chlorophenolica foi acompanhada por testes de bioluminescência de Escherichia coli HB101 pUCD607 e contagens bacterianas no solo e nas raízes. A degradação de PCP ocorreu de forma mais rápida no solo plantado e inoculado quando comparada ao solo sem plantas. Houve um aumento significativo nas populações dos organismos testados nas raízes quando comparadas com as populações presentes no solo. O monitoramento do crescimento da planta mostrou o papel protetor exercido pela S.chlorophenolica contra a toxicidade do PCP.
Environmental Technology
Chlorophenols are well-known priority pollutants and many different treatments have been assessed... more Chlorophenols are well-known priority pollutants and many different treatments have been assessed to facilitate their removal from industrial wastewater. However, an absolute and optimum solution still has to be practically implemented in an industrial setting. In this work, a series ofphysical, chemical and biochemical treatments have been systematically tested for the removal of 4-chlorophenol, and their results have been compared in order to determine the most effective treatment based on removal efficiency and residual by-product formation. Chemical treatments based on advanced oxidation processes (AOP) produced the best results on rate and extent of pollutant removal. The non-chemical technologies showed advantages in terms of complete (in the case of adsorption) or easy (enzymatic treatments) removal of toxic treatment by-products. The AOP methods led to the production of different photoproducts depending on the chosen treatment. Toxic products remained in most cases following...
Environmental technology, Jan 26, 2016
The aim of this research was to estimate the production of hydrogen, organic acids and alcohols b... more The aim of this research was to estimate the production of hydrogen, organic acids and alcohols by the strain of Clostridium acetobutylicum ATCC 824 using residual glycerol as a carbon source. The experiments were carried out in pure and mixed cultures in batch experiments. Three different sources of inocula for mixed culture were used. Ruminal liquid from goats and sludge collected from two upflow anaerobic sludge blanket reactors treating municipal wastewater and brewery effluent were tested for hydrogen, organic acids and alcohols production with or without C. acetobutylicum ATCC 824. The main detected end-products from the glycerol fermentation were hydrogen, organic acids (acetic, propionic, butyric and caproic) and alcohol (ethanol and 1,3-propanediol - 1,3PD). High hydrogen (0.44 mol H2/mol glycerol consumed) and 1,3PD (0.32 mol 1,3PD/mol glycerol consumed) yields were obtained when the strain C. acetobutylicum ATCC 824 was bioaugmented into the sludge from municipal wastewat...
Boletim do Centro de Pesquisa de Processamento de Alimentos, 2009
Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de bactéri... more Avaliou-se a qualidade sanitária de pescadinha (Cyanoscian striatus) mediante pesquisa de bactérias patógenas e de microrganismos indicadores de contaminação, em amostras colhidas na forma inteira e em filés, “in natura” e congeladas. Em 80% das amostras de filé e 40% do pescado inteiro “in natura”, constatou-se Salmonella spp, situando-se a contagem padrão de psicrotróficos entre limites aceitáveis pelo ICMSF. Não se constatou presença de bactérias coliformes fecais, apenas coliformes totais e número não significativo de Staphylococcus aureus. Os resultados indicam deficiência de higiêne e sanificação nas operações rotineiras do processamento.
Engenharia Sanitaria e Ambiental, 2007
O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenol... more O principal objetivo deste trabalho foi estudar a degradação de PCP por Sphingomonas chlorophenolicaem solo argiloso na presença e ausência de trigo. As concentrações de PCP foram determinadas através de Análises de Alta Performance de Cromatografia Líquida. Os efeitos tóxicos de PCP foram estudados através do monitoramento do crescimento das plantas. A biodegradação de PCP por S. chlorophenolica foi acompanhada por testes de bioluminescência de Escherichia coli HB101 pUCD607 e contagens bacterianas no solo e nas raízes. A degradação de PCP ocorreu de forma mais rápida no solo plantado e inoculado quando comparada ao solo sem plantas. Houve um aumento significativo nas populações dos organismos testados nas raízes quando comparadas com as populações presentes no solo. O monitoramento do crescimento da planta mostrou o papel protetor exercido pela S.chlorophenolica contra a toxicidade do PCP.
Boletim do Centro de Pesquisa de Processamento de Alimentos, 2009
Apresenta revisão bibliográfica sobre o Sistema de Análise de Risco e Pontos Críticos de Controle... more Apresenta revisão bibliográfica sobre o Sistema de Análise de Risco e Pontos Críticos de Controle. Comenta a origem do sistema e a sua importância na garantia da qualidade e segurança do produto alimentício. São discutidas as suas aplicações na indústria de alimentos, mostrando seus objetivos e elementos, além de enfatizar os procedimentos necessários para sua implantação.
Boletim do Centro de Pesquisa de Processamento de Alimentos, 2009
Elaborou-se Manual de Boas Práticas de Higiene e Sanificação para Indústria de Pescado Congelado ... more Elaborou-se Manual de Boas Práticas de Higiene e Sanificação para Indústria de Pescado Congelado com o objetivo de orientar, de forma simples e prática os manipuladores e todos os envolvidos no processamento do pescado, quanto às práticas de higiene e de sanificação necessárias para a obtenção de produto de qualidade. Para tanto montou-se fluxograma do processamento de congelamento da indústria de pescado congelado, identificando-se os principais pontos críticos de controle. Levantou-se as condições higiênico-sanitárias dos equipamentos, utensílios e processamento, assim como os hábitos higiênicos dos manipuladores. Acredita-se que o uso do Manual possa propiciar sanificação e condições adequadas de higiene para o trabalho, garantindo a qualidade do produtos.
Pesticidas: Revista de Ecotoxicologia e Meio Ambiente, 2007
O objetivo deste trabalho foi estudar a degradação de pentaclorophenol (PCP) por S. chlorophenoli... more O objetivo deste trabalho foi estudar a degradação de pentaclorophenol (PCP) por S. chlorophenolica em dois diferentes tipos de solo (arenoso e argiloso) na presença e ausência de plantas (trigo - Triticum aestivum). As concentrações de PCP foram determinadas mediante Cromatografia a Líquido de Alta Eficiência (CLAE). Os efeitos tóxicos de PCP foram estudados pelo monitoramento do crescimento das plantas (em peso, g) e medidas das raízes (cm). A biodegradação de PCP por S. chlorophenolica nos dois tipos de solo foi acompanhada por análises de bioluminescência de Escherichia coli HB101 pUCD607. Contagens bacterianas foram realizadas em três meios de cultura: meio mineral para S. chlorophenolica, meio mineral para organismos degradadores/tolerantes ao PCP e ágar triptose caldo de soja para organismos heterotróficos. No solo argiloso com vegetação, a degradação de PCP ocorreu de forma mais rápida após a introdução de S. chlorophenolica que no solo sem plantas. O monitoramento do cresc...
Separation Science and Technology, 2010
4-Chlorophenol removal combining KrCl excimer UV lamp and H2O2 has been studied, using 4-chloroph... more 4-Chlorophenol removal combining KrCl excimer UV lamp and H2O2 has been studied, using 4-chlorophenol concentrations of 100 and 250 mg L and molar ratios H2O2: 4-chlorophenol = 1:1, 10:1, 25:1, and 50:1. A ratio of 25:1 achieves the total removal for both 4-chlorophenol and the photoproducts. Comparing these results with those corresponding to the use of excilamp treatment in the absence of H2O2, there
Journal of Hazardous Materials, 2011
The effect of silver ions, nano-and micro-particles on a luminescent biosensor bacterium Pseudomo... more The effect of silver ions, nano-and micro-particles on a luminescent biosensor bacterium Pseudomonas putida originally isolated from activated sludge was assessed. The bacterium carrying a stable chromosomal copy of the lux operon (luxCDABE) was able to detect toxicity of ionic and particulate silver over short term incubations ranging from 30 to 240 min. The IC 50 values obtained at different time intervals showed that highest toxicity (lowest IC 50) was obtained after 90 min incubation for all toxicants and this is considered the optimum incubation for testing. The data show that ionic silver is the most toxic followed by nanosilver particles with microsilver particles being least toxic. Release of nanomaterials is likely to have an effect on the activated sludge process as indicated by the study using a common sludge bacterium involved in biodegradation of organic wastes.
Journal of Hazardous Materials, 2009
Time-course performance of a phenol-degrading indigenous bacterial consortium, and of Acinetobact... more Time-course performance of a phenol-degrading indigenous bacterial consortium, and of Acinetobacter calcoaceticus var. anitratus, isolated from an industrial coal wastewater treatment plant was evaluated. This bacterial consortium was able to survive in the presence of phenol concentrations as high as 1200 mg L −1 and the consortium was more fast in degrading phenol than a pure culture of the A. calcoaceticus strain. In a batch system, 86% of phenol biodegradation occurred in around 30 h at pH 6.0, while at pH 3.0, 95.2% of phenol biodegradation occurred in 8 h. A high phenol biodegradation (above 95%) by the mixed culture in a bioreactor was obtained in both continuous and batch systems, but when test was carried out in coke gasification wastewater, no biodegradation was observed after 10 days at pH 9-11 for both pure strain or the isolated consortium. An activated sludge with the same bacterial consortium characterized above was mixed with a textile sludge-contaminated soil with a phenol concentration of 19.48 mg kg −1. After 20 days of bioaugmentation, the remanescent phenol concentration of the sludge-soil matrix was 1.13 mg kg −1 .
International Biodeterioration & Biodegradation, 2007
Sphingobium chlorophenolicum is well known as pentachlorophenol (PCP) degrader. The objective of ... more Sphingobium chlorophenolicum is well known as pentachlorophenol (PCP) degrader. The objective of this study was to evaluate the PCP degradation in soil and hydroponic systems using a bioaugmentation technique. Measurements of PCP concentrations were carried out using high-performance liquid chromatography (HPLC) analyses. The toxic effect of PCP on plants was studied through the monitoring of plant weight. An acute toxicity
Environmental Technology, 2012
This article may be used for research, teaching, and private study purposes. Any substantial or s... more This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae, and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand, or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.
Chemosphere, 2007
Sphingobium chlorophenolicum is well known as a pentachlorophenol (PCP) degrader. The objective o... more Sphingobium chlorophenolicum is well known as a pentachlorophenol (PCP) degrader. The objective of this study was to evaluate PCP degradation in a loamy sandy soil artificially contaminated with PCP using phytoremediation and bioaugmentation. Measurements of PCP concentrations were carried out using high performance liquid chromatography analyses (HPLC). The toxic effect of PCP on plants was studied through the monitoring of weight plant and root length. The biodegradation of PCP by S. chlorophenolicum in soil was assessed with a bioluminescence assay of Escherichia coli HB101 pUCD607. Bacterial analyses were carried out by plating on Mineral Salt Medium (MSM) for S. chlorophenolicum, MSM for PCP-degrading/tolerant organisms and Trypticase Soy Broth Agar (TSBA) for heterotrophic organisms. The introduction of S. chlorophenolicum into soil with plants showed a faster degradation when compared to the non-inoculated soil. The monitoring of the plant growth showed a protective role of S. chlorophenolicum against the toxicity of PCP. The bioassay confirmed that initial toxicity was lowered while degradation progressed. There was a significant increase of organisms tested in the roots in comparison to those in the soil. This study showed that the presence of S. chlorophenolicum enhanced the PCP degradation in a loamy soil and also it had a protective role to prevent phytotoxic effects of PCP on plant growth. The combined use of bioaugmentation and plants suggests that the rhizosphere of certain plant species may be important for facilitating microbial degradation of pesticides in soil with important implications for using vegetation to stabilize and remediate surface soils.