Dan Gheban - Academia.edu (original) (raw)

Papers by Dan Gheban

Antioxidants, Oct 22, 2019

We have investigated the cardio-protective effects of pretreatment with curcumin nanoparticles (C... more We have investigated the cardio-protective effects of pretreatment with curcumin nanoparticles (CUN) compared to conventional curcumin (CUS) on the changes in oxidative stress parameters and inflammatory cytokine levels during induced acute myocardial infarction (AMI) in rats with diabetes mellitus (DM). DM was induced with streptozotocin, and AMI with isoproterenol. Eight groups of seven Wister Bratislava rats were included in the study. The N-C was the normal control group, AMI-C was the group with AMI, DM-C was the group with DM, and DM-AMI-C was the group with DM and AMI. All four groups received saline solution orally during the whole experiment. S-DM-CUS-AMI and S-DM-CUN-AMI groups received saline for seven days prior to DM induction and continued with CUS (200 mg/kg bw, bw = body weight) for S-DM-CUS-AMI and CUN for S-DM-CUN-AMI (200 mg/kg bw) for 15 days before AMI induction. The CUS-DM-CUS-AMI group received CUS (200 mg/kg bw), while the CUN-DM-CUN-AMI received CUN (200 mg/kg bw) for seven days prior to DM induction, and both groups continued with administration in the same doses for 15 days before AMI induction. CUS and CUN prevented elevation of creatine kinase, creatine kinase-MB, lactate dehydrogenase in all groups, with better results in the CUN (S-DM-CUN-AMI and CUN-DM-CUN-AMI groups). CUS and CUN significantly reduced serum levels of oxidative stress markers (malondialdehyde, the indirect assessment of nitric oxide synthesis, and total oxidative status) and enhanced antioxidative markers (total antioxidative capacity and thiols, up to 2.5 times). All groups that received CUS or CUN showed significantly lower serum levels of tumor necrosis factor-alpha, interleukin-6, and interleukin-1β. The best antioxidative and anti-inflammatory effects were obtained for the group that received CUN before DM induction (CUN-DM-CUN-AMI group). Pretreatment with CUN proved higher cardio-protective effects exerting an important antioxidative and anti-inflammatory impact in the case of AMI in DM.

International Journal of Molecular Sciences, Oct 3, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Antioxidants, Mar 18, 2023

Romanian Journal of Pediatrics

Background. The clinical characteristics of patients with celiac disease cover a wide range of si... more Background. The clinical characteristics of patients with celiac disease cover a wide range of signs and symptoms. Our study aimed to describe the main clinical features and investigations performed on our celiac patients diagnosed in the past five years. Material and methods. We collected data from medical records of patients aged 0 to 18 years diagnosed with celiac disease. We included in the analysis patients newly diagnosed with celiac disease, in our department, between January 2017 and August 2022. Results. In this period, 30 children were diagnosed with celiac disease in our general pediatrics department. The mean age at diagnosis ± standard deviation was 6.3±4.2 years (median age=4.3 years). The period between onset of symptoms ranged between 3 months to 5 years (median=6 months). Twenty children (66%) had features of classical celiac disease. Thirteen/30 patients (43.3%) were found to have iron deficiency anemia at diagnosis. In 23/30 (76%) of the children, the values of th...

International Journal of Molecular Sciences

The present study evaluated the anti-inflammatory and analgesic effects of conventional curcumin ... more The present study evaluated the anti-inflammatory and analgesic effects of conventional curcumin (cC) and curcumin nanoparticles (nC) associated with diclofenac sodium (D) in experimental acute inflammation (AI) induced by carrageenan administration. Seven groups of eight randomly selected Wistar-Bratislava white rats were evaluated. One group was the control (C), and AI was induced in the other six groups. The AI group was treated with saline solution, the AID group was treated with D, the AIcC200 and AInC200 groups were treated with cC and nC, respectively, while AIcC200D and AInC200D were treated with cC and nC, respectively, both associated with D. Conventional curcumin, nC, and D were administered in a single dose of 200 mg/kg b.w. for cC and nC and 5 mg/kg b.w. for D. Association of cC or nC to D resulted in significant antinociceptive activity, and improved mechanical pressure stimulation and heat thresholds at 3, 5, 7 and 24 h (p < 0.03). The association of cC and nC with...

Варненски медицински форум (Varna Medical Forum), Dec 20, 2012

Obstetrica şi Ginecologia, Oct 28, 2021

Romanian Journal of Pediatrics, 2012

Boala Kikuchi este o afecţiune rară, benignă şi autolimitată, de etiologie necunoscută, care ar t... more Boala Kikuchi este o afecţiune rară, benignă şi autolimitată, de etiologie necunoscută, care ar trebui inclusă în algoritmul diagnostic al adenopatiei cervicale. Diagnosticul de certitudine este histopatologic, modifi cările specifi ce fi ind necroza paracorticală ganglionară, histiocitele cu nuclei în formă de semilună şi numeroşii corpi apoptotici. Se prezintă cazul unui pacient de 13 ani cu diagnosticul de boală Kikuchi, afecţiune rar descrisă la vârsta pediatrică.

Minerva gastroenterologica e dietologica, 2010

Eosinophilic esophagitis (EoE) is a chronic, eosinophil-predominant inflammatory condition that c... more Eosinophilic esophagitis (EoE) is a chronic, eosinophil-predominant inflammatory condition that can affect esophageal mucosa at any age. Distinguished from gastroesophageal reflux disease in the mid 1990's, it has seemed to be increasingly prevalent, and is usually a manifestation of food allergy. The endoscopic and histologic features are well described. The clinical manifestations vary considerably by age, with adolescents and adults complaining primarily of dysphagia. Younger children may present with pain, vomiting, other evidence for food allergy, or feeding difficulties. Treatment options include swallowed (non-systemic) steroids and dietary antigen elimination, and must be maintained indefinitely due to the extremely high rate of recurrence off therapy. The complications of untreated disease include fibrosis of the esophageal lamina propria and stricture formation that result in chronic dysphagia, risking food impaction and perforation.

International Journal of Celiac Disease, 2018

Growth is a very reliable index of health assessment, both in children and teenagers. Growth is o... more Growth is a very reliable index of health assessment, both in children and teenagers. Growth is often affected (slowed or arrested) in children who suffer from chronic diseases, including the gastrointestinal disorders. Since the use of the serologic markers and small intestinal biopsy as diagnostic tools, it has become evident that the clinical presentation is highly variable in patients with celiac disease. Some patients present the classic symptoms of the celiac disease (diarrhea, malnutrition and growth failure), while others, unveil their disorder at puberty when growth failure and delayed puberty seem to be the only symptoms of the disease. We present the clinical case of a 16 years old girl, referred to our endocrine unit for short stature, failure to thrive and lack of menarche.

Medicine and Pharmacy Reports, 2020

We present a case of megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS) in a four... more We present a case of megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS) in a four days old female infant who presented with abdominal distension, bilious vomiting, massive hematuria and feeding intolerance which was first interpreted as Prune Belly Syndrome (PBS), referred to our department after iatrogenic gastric and colonic perforation. Berdon syndrome or MMIHS is a rare congenital anomaly characterized by a massive enlarged bladder, distended abdomen, microcolon, functional obstruction of the gastrointestinal tract, and malrotation.

Medicine and Pharmacy Reports, Jul 31, 2016

The article reviews the intestinal ischemia theme on newborn and children. The intestinal ischemi... more The article reviews the intestinal ischemia theme on newborn and children. The intestinal ischemia may be either acute-intestinal infarction (by vascular obstruction or by reduced mesenteric blood flow besides the occlusive mechanism), either chronic. In neonates, acute intestinal ischemia may be caused by aortic thrombosis, volvulus or hypoplastic left heart syndrome. In children, acute intestinal ischemia may be caused by fibromuscular dysplasia, volvulus, abdominal compartment syndrome, Burkitt lymphoma, dermatomyositis (by vascular obstruction) or familial dysautonomia, Addison's disease, situs inversus abdominus (intraoperative), burns, chemotherapy administration (by nonocclusive mesenteric ischemia). Chronic intestinal ischemia is a rare condition in pediatrics and can be seen in abdominal aortic coarctation or hypoplasia, idiopathic infantile arterial calcinosis.

Neurotoxicity Research, Dec 1, 2022

Bisphenol A (BPA) exposure can be associated with neurodevelopmental disorders due to impairment ... more Bisphenol A (BPA) exposure can be associated with neurodevelopmental disorders due to impairment of cell proliferation and synaptic development. Our study evaluated the effects of melatonin (MEL) on ambulatory activity, lipid peroxidation, cytokines, ERK/NF-kB signaling pathway in hippocampus and frontal lobe, and histopathological changes in the hippocampus of the BPA-treated rats. The animals were divided in 4 groups: control, BPA, BPA + MEL I, BPA + MEL II. MEL I (20 mg/kg b.w), and MEL II (40 mg/kg b.w.) were orally administered for 28 days. In the 29th day, BPA (1 mg/kg b.w) was intraperitoneally administered and, after 24 h, Open Field Test (OFT) and Elevated Plus Maze (EPM), were conducted. The results showed that MEL II group made signi cantly more entries in the open arms of EPM, travelled signi cantly greater distance and spent more time in the central part of OFT. Malondialdehyde levels were diminished by MEL II in the hippocampus and by MEL I in the frontal lobe. In the hippocampus, MAPK level was signi cantly lowered by both doses of MEL (p < 0.05) while in frontal lobe, only MEL II reduced the MAPK activation. MEL I and II signi cantly decreased the γH2AX and upregulated the NFkB and pNFkB expressions in the hippocampus while MEL II downregulated the MCP1 expression. Both doses of MEL attenuated the BPA-evoked histopathological alterations in the hippocampus. These data indicate that MEL can mediate the neuroprotection against BPA-induced neurotoxicity and improves the behavioral changes suggesting a real potential as protective agent in brain toxicity.

Journal of Thrombosis and Haemostasis, Mar 1, 2019

protease-activated receptor 4 upregulation, increased platelet aggregation, and coronary lipid de... more protease-activated receptor 4 upregulation, increased platelet aggregation, and coronary lipid deposits induced by long-term dabigatran administrationresults from a diabetes animal model.

Medicina

COVID-19-associated coinfections increase the patient’s risk of developing a severe form of the d... more COVID-19-associated coinfections increase the patient’s risk of developing a severe form of the disease and, consequently, the risk of death. The term “flurona” was proposed to describe the coinfection of the influenza virus and SARS-CoV-2. This report is about a case of a 7-month-old female infant who died due to flurona coinfection. A histopathological exam showed activation of microglia (becoming CD45 positive), bronchial inflammation, diffuse alveolar damage in proliferative phase with vasculitis, a peribronchial infiltrate that was predominantly CD20-positive, and a vascular wall infiltrate that was predominantly CD3-positive. The aggressiveness of the two respiratory viruses added up and they caused extensive lung inflammation, which led to respiratory failure, multiple organ failure, and death. Tissues injuries caused by both the influenza virus and SARS-CoV-2 could be observed, without the ability to certify the dominance of the aggression of one of the two viruses.

Anatomia, Histologia, Embryologia

The pineal gland is a small‐sized, photo neuroendocrine organ in the midline of the brain that sy... more The pineal gland is a small‐sized, photo neuroendocrine organ in the midline of the brain that synthesises and secretes melatonin and serotonin. Chords and islands of pinealocytes constitute the secretory parenchyma, while glial tissue and calcifications represent degenerative changes. This study examined human postmortem pineal glands to microscopically assess morphological changes possibly associated with clinical data, by using digital techniques. A retrospective autopsy study has been performed on 72 paediatric and adult autopsy cases. The glands have been processed for histological analysis and immunohistochemical staining with synaptophysin (SYN), neuron‐specific enolase (NSE), and neurofilament (NF). Slides were digitally scanned. Morphometric data were obtained using CaseViewer and ImageJ. The comorbidities used for correlation with morphometric data were obesity, type 2 diabetes, adrenal gland adenoma, goitre, chronic pancreatitis, arterial hypertension, and mixed dementia....

Medicina

We report the case of a 34-year-old male patient, a bodybuilding trainer and user of anabolic and... more We report the case of a 34-year-old male patient, a bodybuilding trainer and user of anabolic androgenic steroids (AASs) for 16 years. He was found in cardio-respiratory arrest in his home. By performing a medico-legal autopsy, a severe form of COVID-19, aortic atherosclerotic plaques, and an old myocardial infarction was found. The SARS-CoV-2 RT-PCR test on necroptic lung fragments was positive, with a B.1.258 genetic line. The histopathological examinations showed microthrombi with endothelitis in the cerebral tissue, massive pulmonary edema, diffuse alveolar damage grade 1, pulmonary thromboembolism, hepatic peliosis, and severe nesidioblastosis. The immunohistochemical examinations showed SARS-CoV-2 positive in the myocardium, lung, kidneys, and pancreas. ACE-2 receptor was positive in the same organs, but also in the spleen and liver. HLA alleles A*03, A*25, B*18, B*35, C*04, C*12, DRB1*04, DRB1*15, DQB1*03, DQB1*06 were also identified. In conclusion, death was due to a geneti...

Antioxidants, Oct 22, 2019

International Journal of Molecular Sciences, Oct 3, 2022

Antioxidants, Mar 18, 2023

Romanian Journal of Pediatrics

International Journal of Molecular Sciences

Варненски медицински форум (Varna Medical Forum), Dec 20, 2012

Obstetrica şi Ginecologia, Oct 28, 2021

Romanian Journal of Pediatrics, 2012

Minerva gastroenterologica e dietologica, 2010

International Journal of Celiac Disease, 2018

Medicine and Pharmacy Reports, 2020

Medicine and Pharmacy Reports, Jul 31, 2016

Neurotoxicity Research, Dec 1, 2022

Journal of Thrombosis and Haemostasis, Mar 1, 2019

Medicina

Anatomia, Histologia, Embryologia

Medicina

Viral glossitis is an uncommon condition in an immunocompetent patient. We reported a patient wit... more Viral glossitis is an uncommon condition in an immunocompetent patient. We reported a patient with developed painful pseudo-membranes on the tongue. The diagnosis showed multiple viral infections. The patient promptly responded to antiviral therapy. Multiple viral infections should no longer be considered as a diagnosis only in immunosuppressed patients, but in healthy persons as well.