Dana Smith - Academia.edu (original) (raw)

Papers by Dana Smith

Oral Cavity Reconstruction, 2005

Geriatric Otolaryngology, 2006

International Journal of Pediatric Otorhinolaryngology, 2004

Two case reports of PHACE syndrome (posterior fossa malformations (P), hemangiomas (H), arterial ... more Two case reports of PHACE syndrome (posterior fossa malformations (P), hemangiomas (H), arterial anomalies (A), coarctation of the aorta and cardiac defects (C), and eye abnormalities (E)) are presented. Clinical characteristics consisted of cutaneous and airway hemangiomas, aortic coarctation, and left superior vena cava in one child and cutaneous and airway hemangiomas, sternal clefting, and supraumbilical raphe in the other child. Treatment modalities included systemic and intralesional steroids for cutaneous and airway hemangiomas, submucosal resection and laryngotracheal reconstruction for airway hemangiomas, repair of aortic coarctation, and laser treatment of cutaneous hemangiomas. PHACE syndrome poses a significant potential for airway compromise from hemangiomas, which may require multimodality treatment.

Otolaryngology-head and Neck Surgery, 2005

Treatment of severe epistaxis can encompass many modalities. Control rates with all treatments ar... more Treatment of severe epistaxis can encompass many modalities. Control rates with all treatments are good. Morbidity among treatment groups varies. Angiographic embolization is one such method that has a very low complication rate. Over the last 10 years, it has become the preferred treatment at our institution.Tertiary medical referral centers: OHSU, Portland VAMC.Retrospective review of 70 patients transferred or admitted with posterior epistaxis and treated with selective angiographic embolization from 1993 to 2002.Patients had bleeding for a median of 4.5 days prior to admission. 79% were unilateral. Etiology of bleeding was: idiopathic (61%), previous surgery (11%), anticoagulants (9%), trauma (7%), and other causes (12%). 30% required blood transfusions prior to admission to OHSU (average 4.4 units). No patient required a transfusion postoperatively following angiographic embolization or during their hospitalization. The internal maxillary artery (IMAX) was embolized in 94% (47% unilateral or bilateral IMAX only, 47% unilateral or bilateral IMAX in combination with other vessels, 6% other vessels besides the IMAX). Mean length of stay was 2.5 days. 86% had minor or no complications after the embolization and were discharged within 24 hours. 13% had a major rebleed that required surgical intervention within 6 weeks of the embolization. One patient had a serious neurological complication. Using the data available on 68 of 70 patients, the cost of hospitalization averaged 18,000withdirectcostsofembolizationaveraging18,000 with direct costs of embolization averaging 18,000withdirectcostsofembolizationaveraging11,000.Angiographic embolization is a clinically effective treatment for severe epistaxis.C.

The Cleft Palate-Craniofacial Journal, 2006

To compare speech outcomes after operative intervention for velopharyngeal insufficiency between ... more To compare speech outcomes after operative intervention for velopharyngeal insufficiency between velocardiofacial syndrome patients and nonsyndromic patients. Retrospective cohort study. Tertiary academic center. Cohorts of 14 velocardiofacial syndrome and 15 nonsyndromic patients without overt cleft palate who underwent operative procedures to correct velopharyngeal insufficiency. All velocardiofacial syndrome patients were positive for 22q11.2 microdeletion by fluorescent in situ hybridization and possessed phenotypic features of velocardiofacial syndrome. Operative procedures, including sphincter pharyngoplasty, Furlow palatoplasty, or both, were selected based on preoperative endoscopic assessments of velopharyngeal motion and residual gap size and shape, as well as velocardiofacial syndrome status. Five single and 9 combined procedures were performed in the velocardiofacial syndrome group, whereas 13 single and 2 combined procedures were performed in the nonsyndromic group. Pre- and post-op evaluation was conducted by a speech pathologist. Assessment parameters scored on a numerical scale included speech intelligibility, resonance, nasal air emissions, and overall severity of velopharyngeal insufficiency. Postoperative complications were recorded. Most velocardiofacial syndrome patients and nonsyndromic patients demonstrated significant improvements in all parameters. Comparison of the two groups demonstrated similar improvements in both. Changes in speech resonance were significantly different between the two groups, whereas other speech parameters did not reach significance. There was no difference in airway complications between groups. Velocardiofacial syndrome patients may have comparable outcomes to nonsyndromic patients in selective surgical management of velopharyngeal insufficiency. In addition, the data demonstrate the efficacy of a single-stage combined procedure without increased morbidity.

Molecular Biology of The Cell, 2004

Mutation of the template region in the RNA component of telomerase can cause incorporation of mut... more Mutation of the template region in the RNA component of telomerase can cause incorporation of mutant DNA sequences at telomeres. We made all 63 mutant sequence combinations at template positions 474-476 of the yeast telomerase RNA, TLC1. Mutants contained faithfully incorporated template mutations, as well as misincorporated sequences in telomeres, a phenotype not previously reported for Saccharomyces cerevisiae telomerase template mutants. Although growth rates and telomere profiles varied widely among the tlc1 mutants, chromosome separation and segregation were always aberrant. The mutants showed defects in sister chromatid separation at centromeres as well as telomeres, suggesting activation of a cell cycle checkpoint. Deletion of the DNA damage response genes DDC1, MEC3 or DDC2/SML1 failed to restore chromosome separation in the tlc1 template mutants. These results suggest that mutant telomere sequences elicit a checkpoint that is genetically distinct from those activated by deletion of telomerase or DNA damage.

Accurate chromosome segregation requires the precise coordination of events during the cell cycle... more Accurate chromosome segregation requires the precise coordination of events during the cell cycle. Replicated sister chromatids are held together while they are properly attached to and aligned by the mitotic spindle at metaphase. At anaphase, the links between sisters must be promptly dissolved to allow the mitotic spindle to rapidly separate them to opposite poles. To isolate genes involved in chromosome behavior during mitosis, we microscopically screened a temperature-sensitive collection of budding yeast mutants that contain a GFP-marked chromosome. Nine LOC (loss of cohesion) complementation groups that do not segregate sister chromatids at anaphase were identified. We cloned the corresponding genes and performed secondary tests to determine their function in chromosome behavior. We determined that three LOC genes, PDS1, ESP1, and YCS4, are required for sister chromatid separation and three other LOC genes, CSE4, IPL1, and SMT3, are required for chromosome segregation. We isolated alleles of two genes involved in splicing, PRP16 and PRP19, which impair ␣-tubulin synthesis thus preventing spindle assembly, as well as an allele of CDC7 that is defective in DNA replication. We also report an initial characterization of phenotypes associated with the SMT3/SUMO gene and the isolation of WSS1, a highcopy smt3 suppressor. son 1994; Hirano et al. 1997). The budding yeast 1 Present address: Division of Basic Sciences, Fred Hutchinson Cancer condensin complex contains two related Smc protein Research Ctr., Seattle, WA 98109. homologs, Smc2 and Smc4, as well as three regulatory 2 Present address: Department of Molecular and Cell Biology, Harvard proteins, Brn1, Ycs4, and Ycs5 (Strunnikov et al. 1995;

The spindle checkpoint arrests the cell cycle at metaphase in the presence of defects in the mito... more The spindle checkpoint arrests the cell cycle at metaphase in the presence of defects in the mitotic spindle or in the attachment of chromosomes to the spindle. When spindle assembly is disrupted, the budding yeast mad and bub mutants fail to arrest and rapidly lose viability. We have cloned the MAD2 gene, which encodes a protein of 196 amino acids that remains at a constant level during the cell cycle. Gel filtration and co-immunoprecipitation analyses reveal that Mad2p tightly associates with another spindle checkpoint component, Mad1p. This association is independent of cell cycle stage and the presence or absence of other known checkpoint proteins. In addition, Mad2p binds to all of the different phosphorylated isoforms of Mad1p that can be resolved on SDS-PAGE. Deletion and mutational analysis of both proteins indicate that association of Mad2p with Mad1p is critical for checkpoint function and for hyperphosphorylation of Mad1p.

Journal of Cell Biology, 2000

We show that MAD3 encodes a novel 58-kD nuclear protein which is not essential for viability, but... more We show that MAD3 encodes a novel 58-kD nuclear protein which is not essential for viability, but is an integral component of the spindle checkpoint in budding yeast. Sequence analysis reveals two regions of Mad3p that are 46 and 47% identical to sequences in the NH 2 -terminal region of the budding yeast Bub1 protein kinase. Bub1p is known to bind Bub3p and we use two-hybrid assays and coimmunoprecipitation experiments to show that Mad3p can also bind to Bub3p. In addition, we find that Mad3p interacts with Mad2p and the cell cycle regulator Cdc20p. We show that the two regions of homology between Mad3p and Bub1p are crucial for these interactions and identify loss of function mutations within each domain of Mad3p. We discuss roles for Mad3p and its interactions with other spindle checkpoint proteins and with Cdc20p, the target of the checkpoint.

Science, 1999

20. Resident colonic lamina propria T cells did not respond to CD3 stimulation in vitro nor did t... more 20. Resident colonic lamina propria T cells did not respond to CD3 stimulation in vitro nor did they respond to int280 alone over a dose range of 1 to 25 g/ml, but they did give a proliferative response to Con A (5 g/ml). This response was not enhanced by int280. However, int280 strongly costimulates T cells from the colonic lymphoid follicles. In response to CD3 activation [details as in ], colonic lymphoid follicle T cells gave 9097 Ϯ 1327 cpm, but in the presence of int280 the CD3 response increased to 30,252 Ϯ 4678 cpm.

Handbook of Parenting: Theory and Research for Practice, 2004

Proceedings of the 2009 Winter Simulation Conference (WSC), 2009

... The only way to improve this situation is to become profoundly more productive with the ... W... more ... The only way to improve this situation is to become profoundly more productive with the ... While most people in the industry are using scheduling extensively during construction, the ability ... a sometimes rather violent way, but there are also more tame simulation games that begin ...

Journal of Emotional and Behavioral Disorders, 2004

In this study, the author examined the impact of youth and family preplacement risk factors on mu... more In this study, the author examined the impact of youth and family preplacement risk factors on multidimensional treatment foster care (MTFC) parent—youth interactions,youth treatment completion, and outcomes for boy and girl adolescents who have problems with chronic delinquency. In particular, she looked at (a) how levels of preplacement youth and family risk factors for chronically delinquent boys and girls who

Child Maltreatment, 2006

Several studies have highlighted high levels of risk for girls who have been exposed to traumatic... more Several studies have highlighted high levels of risk for girls who have been exposed to traumatic experiences, but little is known about the exact relationship between traumatic experiences and problems with delinquency and health-risking sexual behavior (e.g., precipitory and/or exacerbatory roles). However, numerous short-and long-term detrimental effects have been linked to trauma, delinquency, and health-risking sexual behavior. The utility of diagnostic and experiential trauma measures in predicting the greatest risk for poor outcomes for delinquent girls was examined in this study. Results indicate that the experiential measures of trauma (cumulative and composite trauma scores) significantly predicted adolescent offending and adolescent health-risking sexual behavior, whereas the diagnostic measures of trauma (full and partial diagnostic criteria) did not.

Journal of Emotional and Behavioral Disorders, 2001

Child Maltreatment, 2007

Parental substance use is a well-documented risk for children. However, little is known about spe... more Parental substance use is a well-documented risk for children. However, little is known about specific effects of prenatal and postnatal substance use on child maltreatment and foster care placement transitions. In this study, the authors unpacked unique effects of (a) prenatal and postnatal parental alcohol and drug use and (b) maternal and paternal substance use as predictors of child maltreatment and foster care placement transitions in a sample of 117 maltreated foster care children. Models were tested with structural equation path modeling. Results indicated that prenatal maternal alcohol use predicted child maltreatment and that combined prenatal maternal alcohol and drug use predicted foster care placement transitions. Prenatal maternal alcohol and drug use also predicted postnatal paternal alcohol and drug use, which in turn predicted foster care placement transitions. Findings highlight the potential integrative role that maternal and paternal substance use has on the risk for child maltreatment and foster care placement transitions.

Handbook of Juvenile Forensic Psychology and Psychiatry, 2012