Daniel Guzman - Academia.edu (original) (raw)

Papers by Daniel Guzman

GSTF Journal of Psychology (JPsych), 2016

The Facial Recognition Test is a widely used psychometric instrument for assessing visuoperceptua... more The Facial Recognition Test is a widely used psychometric instrument for assessing visuoperceptual functioning. Only two prior studies have examined the effects of race/ethnicity on this test. Given that the United States has become more culturally diverse since the creation of the test, it is important to re-visit the effects of this demographic variable on performance. Participants were 75 males and 75 females between the ages of 18 and 43 years (M = 21.91, SD = 5.33). Racial/ethnic categories utilized by the U.S. Census Bureau were equally represented. No gender differences were observed. The race/ethnicity main effect was significant. The gender x race/ethnicity interaction was not significant. The data revealed a clear racial/ethnic performance disparity on the Facial Recognition Test.

Biomedicines, 2020

Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increa... more Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increasingly reliable and accessible. Particularly, the identification of circulating cell-free chemical and biochemical substances, cellular and subcellular entities, and extracellular vesicles has demonstrated promising applications in understanding the physiologic and pathologic conditions of an individual. Traditionally, tissue biopsy has been the gold standard for the diagnosis of many diseases, especially cancer. More recently, liquid biopsy for biomarker detection has emerged as a non-invasive or minimally invasive and less costly method for diagnosis of both cancerous and non-cancerous diseases, while also offering information on the progression or improvement of disease. Unfortunately, the standardization of analytical methods to isolate and quantify circulating cells and extracellular vesicles, as well as their extracted biochemical constituents, is still cumbersome, time-consuming, ...

Biomolecules, 2021

Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, progno... more Over the years, multiple biomarkers have been used to aid in disease screening, diagnosis, prognosis, and response to therapy. As of late, protein biomarkers are gaining strength in their role for early disease diagnosis and prognosis in part due to the advancements in identification and characterization of a distinct functional pool of proteins known as proteoforms. Proteoforms are defined as all of the different molecular forms of a protein derived from a single gene caused by genetic variations, alternative spliced RNA transcripts and post-translational modifications. Monitoring the structural changes of each proteoform of a particular protein is essential to elucidate the complex molecular mechanisms that guide the course of disease. Clinical proteomics therefore holds the potential to offer further insight into disease pathology, progression, and prevention. Nevertheless, more technologically advanced diagnostic methods are needed to improve the reliability and clinical applica...

Waste and Biomass Valorization, 2019

The present study determines the best conditions for the fermentation of Schinus molle drupes by ... more The present study determines the best conditions for the fermentation of Schinus molle drupes by the combination of different types of hydrolysis with the search for an adequate yeast strain. Schinus molle seed residues from an essential oil extraction plant (EOEP) have a high potential for ethanol production. Native yeast strains were isolated from the residues and were used to ferment the lignocellulosic residues, along with baker's yeast (Saccharomyces cerevisiae) at 30 °C and pH 5.5 for comparison. Morphological and biochemical characterizations were carried out on the isolated yeast strains. Thermogravimetric and high-performance liquid chromatography analyses were done on the S. molle seeds (fresh and residue) to determine the ethanol production potential. The followed methodology included increasing the sugar content by hydrolysis with chemical (sulphuric acid, acetic acid, and sodium hydroxide), physical (thermal, vacuum, and ultrasound), and enzymatic treatments (amyloglucosidase and α-amylase). Once the optimum combination of yeast-hydrolysis was determined, a comparison of the greenhouse gas emissions between the original and proposed processes was done. The fermentation of the residues might replace methane from uncontrolled decomposition and reduce the solid residues in 50%/day, hence the EOEP global warming potential is reduced by 47%. The yearly income was estimated to increase by USD 2592.50 from 6302.6 L of ethanol produced from the residues.

Neuropsychopharmacology, 2018

The Journal of neuroscience : the official journal of the Society for Neuroscience, Jan 7, 2017

Repeated exposure to cocaine induces lasting epigenetic changes in neurons that promote the devel... more Repeated exposure to cocaine induces lasting epigenetic changes in neurons that promote the development and persistence of addiction. One epigenetic alteration involves reductions in levels of the histone dimethyltransferase, G9a, in nucleus accumbens (NAc) after chronic cocaine administration. This reduction in G9a may enhance cocaine reward since overexpressing G9a in the NAc decreases cocaine-conditioned place preference. Thus, we hypothesized that HSV-mediated G9a overexpression in the NAc shell (NAcSh) would attenuate cocaine self-administration and cocaine-seeking behavior. Instead, we found that G9a overexpression, and the resulting increase in H3K9 histone dimethylation, increases sensitivity to cocaine reinforcement and enhances motivation for cocaine in self-administering male rats. Moreover, when G9a overexpression is limited to the initial 15 days of cocaine self-administration training, it produces an enduring post-expression enhancement in cocaine self-administration, ...

The Journal of neuroscience : the official journal of the Society for Neuroscience, 2017

Cocaine self-administration increases expression of GluA1 subunits in ventral tegmental area (VTA... more Cocaine self-administration increases expression of GluA1 subunits in ventral tegmental area (VTA) dopamine neurons that subsequently enhance the motivation for cocaine. This increase in GluA1 may be dependent on concomitant NMDA receptor (NMDAR) activation during self-administration, similar to cocaine-induced LTP in the VTA. In this study, we used viral-mediated expression of a dominant negative GluN1 subunit (HSV-dnGluN1) in VTA neurons to study the effect of transient NMDAR inactivation on the GluA1 increases induced by chronic cocaine self-administration in male rats. We found that dnGluN1 expression in VTA limited to the 3 weeks of cocaine self-administration prevents the subsequent increase in tissue GluA1 levels when compared to control infusions of HSV-LacZ. Surprisingly, dnGluN1 expression led to an enhancement in the motivation to self-administer cocaine as measured using a progressive ratio reinforcement schedule, and enhanced cocaine seeking measured in extinction/reins...

Applied Neuropsychology: Adult, 2017

The Cognistat is a widely used neurobehavioral screening instrument that addresses functioning ac... more The Cognistat is a widely used neurobehavioral screening instrument that addresses functioning across multiple domains. Unlike many popular neuropsychological tests, the Cognistat does not currently assess learning efficiency for verbal material. The purpose of this study was to develop a screening method for assessing verbal learning efficiency with the Cognistat, investigate the effects of two demographic variables (age and gender) on performance, and to establish cutoff scores for impairment. Participants were 253 volunteers between the ages of 18 and 96 years. Participants were classified into two age groups: 18-64 years and 65 þ years. The data revealed a significant age and gender performance difference. Implications for the present findings and for future research are presented.

Archives of Clinical Neuropsychology, 2015

Objective: Symptoms of depression, a frequent problem in people with multiple sclerosis (MS), may... more Objective: Symptoms of depression, a frequent problem in people with multiple sclerosis (MS), may be directly related to physiological disease processes or a secondary consequence of living with a chronic debilitating illness. This study examined relationships between neuropathology and psychosocial functioning in the experience of depression in MS. Specifically, we hypothesized that measures of microstructural white matter integrity and coping style would interact to predict depression symptoms. Method: 48 participants (33 female) with MS completed a battery of psychosocial measures, including the Chicago Multiscale Depression Inventory and the COPE Inventory. Participants also completed a diffusion tensor imaging scan, and mean fractional anisotropy (FA) was calculated to assess microstructural white matter integrity. Separate regression models were used to analyze the interaction of mean FA with coping style to predict depression. Results: The main effect of mean FA in predicting depression was not statistically significant. However, there was a significant main effect of Active Coping (DR2 ¼ .08; p ¼ .05), and the interaction of FA and Active Coping was also significant (DR2 ¼ .10; p , .05). The total model accounted for 23% of variance in depression symptoms. Conclusion: Consistent with hypotheses, these findings demonstrate an interaction of neural and psychosocial factors in predicting depression among people with MS. Specifically, at lower levels of microstructural white matter integrity, participants showed less depression if they used more Active Coping; at higher levels of white matter integrity, participants displayed low levels of depression regardless of coping style. These findings illustrate that increased use of Active Coping moderates the relationship between structural neuropathology and depression in MS.

Journal of Neurological Surgery Part B: Skull Base, 2016

Introduction Cerebrospinal fluid (CSF) rhinorrhea is a condition in which there is a perforation ... more Introduction Cerebrospinal fluid (CSF) rhinorrhea is a condition in which there is a perforation of the skull base that results in communication between the subarachnoid space and surrounding extracranial spaces leading to leakage of CSF. 1 Common sites of the defect include the frontal, ethmoid, and sphenoid sinuses as well as the cribriform plate. 1,2 Symptoms unique to Keywords ► cerebrospinal fluid rhinorrhea ► CSF rhinorrhea ► Nationwide Inpatient Sample ► NIS ► HCUP ► CSF leak repair ► length of stay ► hospital charges ► complications ► skull base surgery

Journal of Chromatography B, 2016

An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for ... more An emerging micro-scale immuno-analytical diagnostic tool to see the unseen. Holding promise for precision medicine and P4 medicine,

GSTF Journal of Psychology (JPsych), 2014

The purpose of this study was to assess the convergent validity of a single depression question w... more The purpose of this study was to assess the convergent validity of a single depression question with multiple classification options for depression screening. Participants were 40 medical inpatients. The age range of our sample was 18 to 85 years (M = 56.15, SD = 17.66). A clinical interview and the BDI-II were administered. The correlation between patients' self-rating classification of depression and their BDI-II classification was significant, rs(38) = .90, p < .01. Follow-up repeated-measures chisquare revealed a statistically significant association between BDI-II classification and patients' self-rating classification, χ 2 (9, N = 40) = 47.79, p < .005. Significant positive standardized residuals revealed a clear linear relationship between BDI-II and patient self-rating classifications. Our data support the use of a single depression question with multiple classification options as a useful and valid means of quickly screening for the presence of depression by frontline health care professionals.

European Journal of Pharmacology, 2009

In previous work we have shown that rats running a straight alley for intravenous (i.v.) or intra... more In previous work we have shown that rats running a straight alley for intravenous (i.v.) or intracerebroventricular (i.c.v.) injections of cocaine develop an ambivalence about entering the goal box that results from cocaine's mixed reinforcing and anxiogenic properties. What remains unclear is whether or not cocaine's opposing properties stem from actions on a common neuronal system or from dual actions on separate systems-one related to reward and another to anxiogenic responses. One way to address this question is to deliver cocaine into discrete brain areas as a means of assessing whether or not the positive and negative effects of the drug can be spatially dissociated. Given the putative role of mesocorticolimbic dopamine pathways in the mediation of cocaine-reinforced behavior, the current study examined the cocaine-seeking behavior of rats permitted to run an alley once each day for bilateral medial prefrontal cortex microinjections of cocaine (0.0, 12.5, 25 or 50μg/0.5 μl per side) delivered upon goal-box entry. The results demonstrated that undrugged animals are highly motivated to seek medial prefrontal cortex cocaine without any evidence of negative or anxiogenic effects at any dose. These results are therefore consistent with suggestions of a medial prefrontal cortex involvement in the reinforcing actions of cocaine, and indicate that the dual and opposing actions of the drug can be dissociated and hence may be mediated by the drug's actions on separate neuronal systems.

Chemosphere, 2008

The photocatalytic degradation of alazine and gesaprim commercial herbicides was carried out in a... more The photocatalytic degradation of alazine and gesaprim commercial herbicides was carried out in aqueous TiO(2) suspensions under UV light (15W, 352 nm). Degradation of these herbicides was also observed by the combined effects of photocatalysis with sonolysis (sonophotocatalysis) using an ultrasound source of 20kHz. Degradation profiles were recorded by measuring the concentration of the active compounds present in the alazine (alachlor and atrazine) and gesaprim (atrazine) by HPLC as a function of irradiation time (sound and/or light). Over 90% of the active component in the gesaprim was abated and those in alazine were completely degraded. The content of total organic carbon and chemical oxygen demand was also monitored. Mineralization of the commercial herbicides was achieved. Over 80% of chemical oxygen demand abatement was attained for both herbicides with sonophotocatalysis at 150 min of irradiation time. The photocatalytic degradation of the herbicides followed a pseudo-first order kinetics and their rate constant was increased by the combined effects of sonolysis.

Behavioural Pharmacology, 2007

In previous work from our laboratory, animals running for intravenous cocaine developed a unique ... more In previous work from our laboratory, animals running for intravenous cocaine developed a unique approachavoidance 'retreat behavior' that was hypothesized to result from cocaine's well documented reinforcing (positive) and anxiogenic (negative) properties. To assess the role of central mechanisms in producing cocaine's positive and negative effects, we assessed whether or not animals running a straight alley for intracerebroventricular applications of cocaine would produce a similar behavioral profile to that previously observed with intravenous applications. Retreat frequency and location were measured in male Sprague-Dawley rats trained to run an alley for one of four doses of intracerebroventricularadministered cocaine (0, 25, 50 or 100 lg cocaine/ infusion). Testing involved a single trial per day over 14 consecutive days with a single infusion of cocaine delivered upon goal box entry. The 100 and 50 lg intracerebroventricular cocaine groups exhibited significantly higher retreat frequencies than the 25 and 0 lg groups and the nature and magnitude of the behavior was comparable to that previously observed with intravenous cocaine. These results suggest that the intracerebroventricular self-administration of cocaine results in mixed positive and negative consequences and therefore likely stem from the drug's actions within the central nervous system. Behavioural Pharmacology

Archives of Clinical Neuropsychology, 2013

Objective: Reading Disorder (RD) and Disorder of Written Expression (DWE) are among the highest c... more Objective: Reading Disorder (RD) and Disorder of Written Expression (DWE) are among the highest co-occurring learning disabilities in children with Attention-Deficit/Hyperactivity Disorder (ADHD). However, little information is available regarding IQ and achievement tests predicting diagnoses in comorbid groups. The current study examined children diagnosed with ADHD-Inattentive, ADHD-Combined, or ADHD with coexisting RD and/or DWE. Method: Participants included 135 children with ADHD-Inattentive (n ¼ 59), ADHD-Combined (n ¼ 36), and ADHD, RD, and/or DWE (n ¼ 40). Children were 10.2-year old and 66.5% male with an IQ of 100.9. Diagnoses were established in a private practice through comprehensive evaluations, including administration of the Wechsler Intelligence Scale for Children-Fourth Edition (WISC-IV) and Woodcock Johnson Tests of Achievement Third Edition (WJ-III). Results: Discriminate function analysis was used to predict group membership based upon WISC-IV Indexes and WJ-III Broad Reading Cluster scores. An initial analysis examining all three groups provided an overall correct classification rate of 49.6%. The ADHD Inattentive and Combined groups were pooled to form one group in a second discriminate function analysis. Correct classification rates were 71.9% overall, with 75.0% of the ADHD group and 66.7% of the ADHD with RD/DWE group correctly classified. Conclusion: Results suggest the combination of WISC-IV and WJ-III Broad Reading scores are able to provide good classificatory accuracy in differentiating between children with ADHD alone compared with those with ADHD and RD/DWE. Results further suggest that children with ADHD and RD/DWE are at particular risk for poor performance on tasks requiring reading and verbal ability.

Addiction Biology, 2011

Reexposure to cocaine-associated environments promotes relapse to cocaine seeking and represents ... more Reexposure to cocaine-associated environments promotes relapse to cocaine seeking and represents a persistent impediment to successful abstinence. Neurobiological adaptations are thought to underlie the preservation of drug-seeking behavior during protracted withdrawal periods, possibly including changes associated specifically with cocaine-paired contexts. We measured GluR 1 S845 and ERK phosphorylation in rat striatal subregions in an animal model of cocaine relapse. Animals with cocaine self-administration experience and their yoked partners were exposed to extinction conditions for one hour in the drug-paired environmental context after one day or three weeks withdrawal to measure protein phosphorylation induced by the cocainepaired context in the absence of cocaine reinforcement. GluR 1 S845 (an index of PKA activity) and ERK phosphorylation increased in the nucleus accumbens core of self-administering but not yoked animals after three weeks (but not one day) withdrawal, indicating a time-dependent emergence of context-associated protein phosphorylation in this accumbens subregion. In comparison, animals trained to self-administer sucrose displayed a similar increase in ERK, but not GluR 1 S845 , phosphorylation following reexposure to a sucrose-paired environment three weeks later, indicating that GluR 1 S845 phosphorylation did not result solely from lever press behavior per se. In contrast, basal (home cage) GluR 1 S845 phosphorylation was elevated in the nucleus accumbens shell and caudate-putamen after one day or three weeks cocaine withdrawal regardless of context exposure. These results suggest that time-dependent emergence of contextassociated GluR 1 S845 phosphorylation in the nucleus accumbens core may contribute to the persistence of cocaine-seeking behavior, whereas ERK phosphorylation may be a consequence of this behavior.

inpeau.ufsc.br

Mariana Canedo, José Castro, Olga Della Vedova, Daniel Guzman, Lucía Passoni, Ana Petriela, Sandr... more Mariana Canedo, José Castro, Olga Della Vedova, Daniel Guzman, Lucía Passoni, Ana Petriela, Sandra Quiroga, Omar Rodríguez, Silvia Lucifora, Alejandro Ara mcanedo@mdp.edu.ar ... VII Coloquio de Gestión Universitaria de América del Sur