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Papers by Daniel Medynski
FEBS Open Bio, Sep 17, 2018
Native 1-antitrypsin is a 52-kDa glycoprotein that acts as an antiprotease and is the physiologi... more Native 1-antitrypsin is a 52-kDa glycoprotein that acts as an antiprotease and is the physiological inhibitor of neutrophil serine proteases. The main function of 1-antitrypsin is to protect the lung from proteolytic damage induced by inflammation. 1-antitrypsin deficiency is a codominant autosomal disorder caused by pathogenic mutations in SERPINA1 gene, leading to reduced levels of serum 1-antitrypsin. The
Clinical Cancer Research, 2005
Protein Expression and Purification, 2007
Two des-kringle derivatives of human plasminogen, microplasminogen and miniplasminogen, have been... more Two des-kringle derivatives of human plasminogen, microplasminogen and miniplasminogen, have been expressed at high levels as inclusion bodies in Escherichia coli using a T7 expression system. In each case, the isolated inclusion bodies were refolded and purified. A final yield of approximately 10% of total refolded protein was observed in each case. Both refolded molecules were successfully activated to their functional forms, microplasmin and miniplasmin, by the plasminogen activator urokinase. The kinetic properties of the refolded microplasmin and miniplasmin were comparable to full length, native plasmin.
L'invention concerne des procedes destines a produire un polypeptide de plasminogene et de pl... more L'invention concerne des procedes destines a produire un polypeptide de plasminogene et de plasmine de recombinaison replie correctement. Le polypeptide de plasminogene de recombinaison denature est replie en solubilisant premierement le polypeptide avec un chaotrope, et en reduisant et oxydant des agents a un pH eleve, operations suivies par le repliage en presence d'une concentration reduite de chaotrope et la reduction et l'oxydation d'agent en presence d'arginine.
Molecular and Cellular Mechanisms of Anesthetics, 1986
Proceedings of The National Academy of Sciences, 1985
Transducin, a GTP-binding protein involved in phototransduction in the vertebrate retina, belongs... more Transducin, a GTP-binding protein involved in phototransduction in the vertebrate retina, belongs to a family of homologous coupling proteins that also includes G, and G1, the regulatory proteins of adenylate cyclase. Here we Abbreviations: Ta, a subunit of transducin; T /3y, -'y subunit complex of transducin; PDEase, phosphodiesterase, p[NH]ppG, guanosine 5'-[A3,y-imido]triphosphate; R*, photolyzed rhodopsin; G, and Gi, stimulatory and inhibitory coupling proteins of hormone-sensitive adenylate cyclase. cPresent address:
Nucleic Acids Research, 1989
Biochemical and Biophysical Research Communications, 1984
FEBS Open Bio, Sep 17, 2018
Native 1-antitrypsin is a 52-kDa glycoprotein that acts as an antiprotease and is the physiologi... more Native 1-antitrypsin is a 52-kDa glycoprotein that acts as an antiprotease and is the physiological inhibitor of neutrophil serine proteases. The main function of 1-antitrypsin is to protect the lung from proteolytic damage induced by inflammation. 1-antitrypsin deficiency is a codominant autosomal disorder caused by pathogenic mutations in SERPINA1 gene, leading to reduced levels of serum 1-antitrypsin. The
Clinical Cancer Research, 2005
Protein Expression and Purification, 2007
Two des-kringle derivatives of human plasminogen, microplasminogen and miniplasminogen, have been... more Two des-kringle derivatives of human plasminogen, microplasminogen and miniplasminogen, have been expressed at high levels as inclusion bodies in Escherichia coli using a T7 expression system. In each case, the isolated inclusion bodies were refolded and purified. A final yield of approximately 10% of total refolded protein was observed in each case. Both refolded molecules were successfully activated to their functional forms, microplasmin and miniplasmin, by the plasminogen activator urokinase. The kinetic properties of the refolded microplasmin and miniplasmin were comparable to full length, native plasmin.
L'invention concerne des procedes destines a produire un polypeptide de plasminogene et de pl... more L'invention concerne des procedes destines a produire un polypeptide de plasminogene et de plasmine de recombinaison replie correctement. Le polypeptide de plasminogene de recombinaison denature est replie en solubilisant premierement le polypeptide avec un chaotrope, et en reduisant et oxydant des agents a un pH eleve, operations suivies par le repliage en presence d'une concentration reduite de chaotrope et la reduction et l'oxydation d'agent en presence d'arginine.
Molecular and Cellular Mechanisms of Anesthetics, 1986
Proceedings of The National Academy of Sciences, 1985
Transducin, a GTP-binding protein involved in phototransduction in the vertebrate retina, belongs... more Transducin, a GTP-binding protein involved in phototransduction in the vertebrate retina, belongs to a family of homologous coupling proteins that also includes G, and G1, the regulatory proteins of adenylate cyclase. Here we Abbreviations: Ta, a subunit of transducin; T /3y, -'y subunit complex of transducin; PDEase, phosphodiesterase, p[NH]ppG, guanosine 5'-[A3,y-imido]triphosphate; R*, photolyzed rhodopsin; G, and Gi, stimulatory and inhibitory coupling proteins of hormone-sensitive adenylate cyclase. cPresent address:
Nucleic Acids Research, 1989
Biochemical and Biophysical Research Communications, 1984