Daniel Waisberg - Academia.edu (original) (raw)
Papers by Daniel Waisberg
Einstein (São Paulo), 2015
Objective To determine the presence of glycosaminoglycans in the extracellular matrix of connecti... more Objective To determine the presence of glycosaminoglycans in the extracellular matrix of connective tissue from neoplastic and non-neoplastic colorectal tissues, since it has a central role in tumor development and progression. Methods Tissue samples from neoplastic and non-neoplastic colorectal tissues were obtained from 64 operated patients who had colorectal carcinoma with no distant metastases. Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans after proteolysis and electrophoresis. The statistical analysis included mean, standard deviation, and Student'st test. Results The glycosaminoglycans extracted from colorectal tissue showed three electrophoretic bands in agarose gel. Electrospray ionization mass spectrometry showed characteristic disaccharide fragments from glycosaminoglycans, indicating their structural characterization in the tissues analyzed. Some peaks in the electrospray ionization mass spectrometry were not characterized as fragments of sugars, indicating the presence of fragments of the protein structure of proteoglycans generated during the glycosaminoglycan purification. The average amount of chondroitin and dermatan increased in the neoplastic tissue compared to normal tissue (p=0.01). On the other hand, the average amount of heparan decreased in the neoplastic tissue compared to normal tissue (p= 0.03). Conclusion The method allowed the determination of the glycosaminoglycans structural profile in colorectal tissue from neoplastic and non-neoplastic colorectal tissue. Neoplastic tissues showed greater amounts of chondroitin sulphate and dermatan sulphate compared to non-neoplastic tissues, while heparan sulphate was decreased in neoplastic tissues.
Jornal Brasileiro De Pneumologia Publicacao Oficial Da Sociedade Brasileira De Pneumologia E Tisilogia, Jul 1, 2008
Objetivo: Analisar a taxa de aproveitamento de pulmões disponibilizados em São Paulo no ano de 20... more Objetivo: Analisar a taxa de aproveitamento de pulmões disponibilizados em São Paulo no ano de 2006, bem como caracterizar o perfil dos doadores de pulmão deste período. Métodos: Estudo retrospectivo de 497 prontuários de doadores de pulmão, no período de janeiro a dezembro de 2006. Resultados: Não houve oferta de doação de pulmões para transplante em 149 (30%) dos prontuários analisados, sendo excluídos do estudo. A idade média dos 348 doadores eleitos para o estudo foi de 37,4 ± 16,1 anos, e 56,9% deles eram do sexo masculino. As principais causas da morte cerebral dos doadores foram: acidente vascular cerebral (40,5%); trauma cranioencefálico (34,2%); e hemorragia subaracnóidea (10,9%). A grande maioria dos doadores recebia drogas vasoativas (90,5%), sendo que 13,5% haviam apresentado parada cárdio-respiratória. Do ponto de vista infeccioso, o leucograma médio foi de 15.008 ± 6.467 células/mm 3 , 67,8% recebiam antibioticoterapia e 26,1% apresentavam infecção pulmonar. Quase 40% dos doadores apresentavam alterações radiográficas. Apenas 4,9% dos doadores foram aceitos, representando 28 pulmões (taxa de aproveitamento de órgãos de 4%). Os motivos de recusa foram: alteração gasométrica (30,1%); infecção (23,7%); e distância (10,9%). Conclusões: A taxa de aproveitamento de pulmões em nosso meio é baixa quando comparada às taxas médias de aproveitamento mundial. Além disso, mais de 50% das recusas deveram-se à gasometria arterial inadequada e infecção pulmonar. Aliar melhor cuidado ao potencial doador à menor rigidez nos critérios de seleção poderia aumentar a utilização dos órgãos doados.
Revista Brasileira De Anestesiologia, Dec 1, 2007
... Nara de Cássia Mantovani1, Lúcia Maria Martins Zuliani2, Daniela Tiemi Sano2, Daniel Reis Wai... more ... Nara de Cássia Mantovani1, Lúcia Maria Martins Zuliani2, Daniela Tiemi Sano2, Daniel Reis Waisberg3, Israel Ferreira da Silva, TSA4 Jaques ... Dosis adicionales de atra-curio (1/4 de la dosis inicial) fueron administradas para el mantenimiento de bloqueo neuromuscular y la ...
Revista De Medicina, Dec 6, 2009
Waisberg DR, Galvão RC, Galvão FHF, D'Albuquerque LAC. Transplante intestinal em ratos utilizando... more Waisberg DR, Galvão RC, Galvão FHF, D'Albuquerque LAC. Transplante intestinal em ratos utilizando nova técnica de microanastomose sem sutura com cola e molde tubular (Cuff-Glue). Rev Med (São Paulo). 2009 jul.-dez.;88(3/4):207-18. RESUMO: INTRODUÇÃO: A necessidade de sutura microvascular complexa dificulta a pesquisa do transplante intestinal no rato. A técnica de microanastomose com dispositivo tubular (cuff) é simples, porém, a persistência do dispositivo na anastomose desencadeia reação de corpo estranho e trombose. Neste trabalho descrevemos um modelo de transplante intestinal no rato com nova técnica de anastomose sem sutura com cuff e cola. MÉTODO: O enxerto contém o intestino delgado, colo ascendente e pedículo vascular (conduto aortomesentérico e veia porta). Na extremidade da veia e do coto intestinal, fixa-se o cuff, sobre o qual a parede é evertida. No receptor, realiza-se microanastomose aorto-aórtica manual. O cuff é inserido na veia mesentérica superior e fixado por ligadura circular. A margem proximal é levantada, expondo a camada íntima dos vasos, onde se deposita cola. A margem proximal é abaixada, selando a anastomose. As ligaduras fixando o cuff são retiradas possibilitando a exclusão do cuff da anastomose. Após reperfusão do enxerto, o intestino correspondente do receptor é retirado em bloco e se restabelece o trânsito intestinal utilizando a mesma técnica descrita. RESULTADOS: Observou-se adequada reperfusão do enxerto e boa patência das anastomoses. CONCLUSÃO: O modelo descrito simplifica a realização de microanastomose no transplante intestinal em ratos.
The British journal of surgery, 2015
Although anorectal transplantation is a challenging procedure, it is a promising option for patie... more Although anorectal transplantation is a challenging procedure, it is a promising option for patients who have completely lost anorectal function or in whom it failed to develop, as in congenital malformations. The paucity of animal models with which to test functional outcomes was addressed in this study of anorectal manometry in rats. Wistar rats were assigned randomly to four groups: orthotopic anorectal transplantation, heterotopic transplantation, sham operation, or normal control. Bodyweight and anal pressure were measured immediately before and after operation, and on postoperative days 7 and 14. ANOVA and Tukey's test were used to compare results for bodyweight, anal manometry and length of procedure. Immediately after the procedure, mean(s.d.) anal pressure in the orthotopic group (n = 13) dropped from 31·4(13·1) to 1·6(13·1) cmH2 O (P < 0·001 versus both sham operation (n = 13) and normal control (n = 15)), with partial recovery on postoperative day 7 (14·9(13·9) cmH...
Clinics (São Paulo, Brazil), 2012
This study sought to identify the relationship between fibroblast telomerase expression, myofibro... more This study sought to identify the relationship between fibroblast telomerase expression, myofibroblasts, and telomerase-mediated regulatory signals in idiopathic pulmonary fibrosis. Thirty-four surgical lung biopsies, which had been obtained from patients with idiopathic pulmonary fibrosis and histologically classified as usual interstitial pneumonia, were examined. Immunohistochemistry was used to evaluate fibroblast telomerase expression, myofibroblast α-smooth muscle actin expression and the tissue expression of inter leu kin-4, transforming growth factor-β, and basic fibroblast growth factor. The point-counting technique was used to quantify the expression of these markers in unaffected, collapsed, mural fibrosis, and honeycombing areas. The results were correlated to patient survival. Fibroblast telomerase expression and basic fibroblast growth factor tissue expression were higher in collapsed areas, whereas myofibroblast expression and interleukine-4 tissue expression were hig...
Pediatric Emergency Care, May 1, 2011
Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complica... more Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complication of a neck space infection in children associated with high mortality and morbidity. A 3-year-old boy presented with spontaneous massive epistaxis 45 days after a deep neck space infection caused by a peritonsillar abscess. During nasopharyngeal packing, he evolved with cardiac arrest. Intra-arterial angiography was then performed that revealed a large pseudoaneurysm. Endovascular treatment using detachable balloons achieved complete exclusion of the pseudoaneurysm. The child made an uneventful recovery and was discharged with mild left hemiparesis and no deficit of sensory or cognitive functions. Pseudoaneurysms of the internal carotid artery after a deep neck space infection can be associated with delayed and potentially fatal massive epistaxis. Furthermore, a regional (ie, extranasal) blood vessel should be promptly investigated when there are signs of hypovolemic shock. A high level of suspicion and definitive treatment are essential for successful management of these patients.
Revista Brasileira de Anestesiologia, 2007
... 1. Fisioterapeuta; Encarregada do Serviço de Fisioterapia do Hospital Nossa Senhora de Fátima... more ... 1. Fisioterapeuta; Encarregada do Serviço de Fisioterapia do Hospital Nossa Senhora de Fátima da Sociedade Portuguesa de ... implicará índice menor de complicações decorrentes do tempo menor de perma-nência dos pacientes sob ventilação mecânica e abreviação do ...
Pediatric Emergency Care, 2011
Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complica... more Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complication of a neck space infection in children associated with high mortality and morbidity. A 3-year-old boy presented with spontaneous massive epistaxis 45 days after a deep neck space infection caused by a peritonsillar abscess. During nasopharyngeal packing, he evolved with cardiac arrest. Intra-arterial angiography was then performed that revealed a large pseudoaneurysm. Endovascular treatment using detachable balloons achieved complete exclusion of the pseudoaneurysm. The child made an uneventful recovery and was discharged with mild left hemiparesis and no deficit of sensory or cognitive functions. Pseudoaneurysms of the internal carotid artery after a deep neck space infection can be associated with delayed and potentially fatal massive epistaxis. Furthermore, a regional (ie, extranasal) blood vessel should be promptly investigated when there are signs of hypovolemic shock. A high level of suspicion and definitive treatment are essential for successful management of these patients.
Clinics, 2006
A 57-year-old, white woman presented epigastralgia, steatorrhea, and an 8 kg weight loss, with an... more A 57-year-old, white woman presented epigastralgia, steatorrhea, and an 8 kg weight loss, with an onset 1 year previously. Laboratory exams were normal except for the following: (i) presence of anemia (hemoglobin 7.5 g%), (ii) positive test for occult blood in the stool, and (iii) ...
Clinics (São Paulo, Brazil), 2012
We aimed to evaluate whether the inclusion of videothoracoscopy in a pleural empyema treatment al... more We aimed to evaluate whether the inclusion of videothoracoscopy in a pleural empyema treatment algorithm would change the clinical outcome of such patients. This study performed quality-improvement research. We conducted a retrospective review of patients who underwent pleural decortication for pleural empyema at our institution from 2002 to 2008. With the old algorithm (January 2002 to September 2005), open decortication was the procedure of choice, and videothoracoscopy was only performed in certain sporadic mid-stage cases. With the new algorithm (October 2005 to December 2008), videothoracoscopy became the first-line treatment option, whereas open decortication was only performed in patients with a thick pleural peel (>2 cm) observed by chest scan. The patients were divided into an old algorithm (n = 93) and new algorithm (n = 113) group and compared. The main outcome variables assessed included treatment failure (pleural space reintervention or death up to 60 days after medi...
Jornal Vascular Brasileiro, 2010
Mais Serviços Personalizados Artigo texto em Português pdf em Português ReadCube Artigo em XML Re... more Mais Serviços Personalizados Artigo texto em Português pdf em Português ReadCube Artigo em XML Referências do artigo Como citar este artigo Curriculum ScienTI Tradução automática Enviar este artigo por email Indicadores Citado por SciELO Acessos Links relacionados Compartilhar Permalink Jornal Vascular Brasileiro versão impressa ISSN 1677-5449 J. vasc. bras. vol.9 no.1 Porto Alegre 2010 http://dx.
Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia, 2011
To study the functional behavior of the allograft with immunosuppression of pancreatic islets in ... more To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significant...
Revista Brasileira de Cirurgia Cardiovascular, 2010
Academic Leagues (Academic Interest Group) are entities that belong to medical schools and their ... more Academic Leagues (Academic Interest Group) are entities that belong to medical schools and their hospitals. Their goals are research, medical education and health care. The cardiothoracic Surgery League (Liga de Cirurgia Cardiotorácica--LCCT), University of São Paulo Medical School, was founded 12 years ago, aimed at providing undergraduate medical students an opportunity to expand knowledge in cardiothoracic surgery and to take part in scientific studies in the field. To evaluate the experience gained through LCCT implementation. Current students and former members of LCCT were submitted to a specific evaluation form, delivered personally or by email, which provided data on the general opinion regarding LCCT&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s activities, the reasons for answers provided and choice of medical specialties. Furthermore, LCCT&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s total scientific production was evaluated. Most undergraduate medical students 51/65 (78.5%) and former members 94/121 (77.7%) completed the form. The interest in Cardiothoracic Surgery was the most common reason for joining LCCT 44/161 (27.3%). Most students 32/51 (62.7%)--reported their initial expectations were partially met. Almost every member would recommend a fellow medical student to join LCCT 50/51 (98%) and 25/51 (49%) of them participated in a scientific project linked to LCCT. In total, 47 scientific papers were published having LCCT&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s medical students as authors or co-authors during the existence of the league. Of the former members who chose a surgical career, 7/52 (13.4%) chose thoracic or cardiovascular surgery. LCCT has met its goals, since most current and former members recognized its role in their medical training, besides it is a good way to produce scientific initiation.
Acta Cirurgica Brasileira, 2011
To introduce a model of equipment for inhalation anesthesia in rats that offers better control of... more To introduce a model of equipment for inhalation anesthesia in rats that offers better control of both flow and losses of ether during induction, maintenance, and recuperation. The equipment consists of an air compressor with two outlets, a closed glass induction chamber, a glass reservoir for the anesthetic agent, a pediatric inhalation mask, a three-way stopcock, a Y-connector, and urinary catheters. Three hundred Wistar rats (Rattus norvegicus albinus) were given inhalation anesthesia. The evaluated parameters were equipment operation, duration of each phase of anesthesia, corneal reflex, muscular tonus, respiration during induction and maintenance, and volume of anesthesia. The average time taken for induction was 7.3 minutes; the average anesthetic recuperation time was 6.4 minutes. The amount of anesthetic used varied according to the weight of the animal, with the average volume of ether used being 6.5ml/hour. The availability of oxygen (room air) decreased the recuperation time and averted both respiratory depression and insufficient depth of anesthesia. The proposed equipment is practical, inexpensive, and allows for satisfactory control of anesthetic parameters during the entire procedure, making inhalation anesthesia in rats safe and essentially complication free.
Revista de Medicina, 2008
Junqueira JJM, Waisberg DR, Noleto GS, Samano MN, Pego-Fernandes PM, Jatene FB. Análise das carac... more Junqueira JJM, Waisberg DR, Noleto GS, Samano MN, Pego-Fernandes PM, Jatene FB. Análise das características dos pulmões disponibilizados para transplante no Estado de São Paulo. Rev Med (São Paulo). 2008 jan.-mar.;87(1):44-57. RESUMO: Objetivos: Analisar a taxa de aproveitamento de pulmões disponibilizados em São Paulo em 2006, bem como caracterizar o perfi l dos doadores de pulmão deste período. Métodos: Estudo retrospectivo de 497 prontuários de doadores de pulmão, no período de jan/2006 a dez/2006. Resultados: Dos 497 prontuários analisados, 149 (30%) não tiveram os pulmões doados, sendo excluídos do estudo. Dos 348 doadores eleitos para o estudo, 56,9% eram do sexo masculino, com idade média de 37,4±16,1anos.
Xenotransplantation, 2010
Xenotransplantation and multivisceral transplantation are advanced therapeutic methods that still... more Xenotransplantation and multivisceral transplantation are advanced therapeutic methods that still require a scientific basis. There are no experimental models of multivisceral transplantation available, particularly not the monitoring by endoscopy. Here, we describe the endoscopic features in a model of multivisceral xenotransplantation. The distal esophagus, stomach, intestine, colon, liver, pancreas, and the kidneys with a common vascular pedicle were harvested from rabbits and implanted in swine (group I, n = 9) or in rabbits (group II, n = 4). Endoscopy was performed in the stomach, jejunum, and ascending colon at four consecutive time points (immediate after surgery and 10, 90, and 180 min after reperfusion). Lesions were macroscopically graded as mild, moderate, and severe. Biopsies were taken following sacrifice at 180 min after reperfusion. In group I, the stomach, jejunum, and colon manifested a progression of lesions with predominance of mild lesions after 10 min, mild to moderate lesions after 90 min, and moderate to severe lesions after 180 min. In animals from group II, endoscopy showed normal features at all time points after reperfusion. Histopathologic analysis confirmed the diagnosis of hyperacute rejection in group I. Grafts from group II animals presented normal or mild ischemic/reperfusion injury. All animals subjected to multivisceral xenotransplantation showed a progression of endoscopic lesions with time after transplantation, while animals subjected to allotransplantation showed no aberrations in endoscopy. We conclude that endoscopy is a useful tool in the assessment of hyperacute rejection of a xenograft.
Einstein (São Paulo), 2015
Objective To determine the presence of glycosaminoglycans in the extracellular matrix of connecti... more Objective To determine the presence of glycosaminoglycans in the extracellular matrix of connective tissue from neoplastic and non-neoplastic colorectal tissues, since it has a central role in tumor development and progression. Methods Tissue samples from neoplastic and non-neoplastic colorectal tissues were obtained from 64 operated patients who had colorectal carcinoma with no distant metastases. Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans after proteolysis and electrophoresis. The statistical analysis included mean, standard deviation, and Student'st test. Results The glycosaminoglycans extracted from colorectal tissue showed three electrophoretic bands in agarose gel. Electrospray ionization mass spectrometry showed characteristic disaccharide fragments from glycosaminoglycans, indicating their structural characterization in the tissues analyzed. Some peaks in the electrospray ionization mass spectrometry were not characterized as fragments of sugars, indicating the presence of fragments of the protein structure of proteoglycans generated during the glycosaminoglycan purification. The average amount of chondroitin and dermatan increased in the neoplastic tissue compared to normal tissue (p=0.01). On the other hand, the average amount of heparan decreased in the neoplastic tissue compared to normal tissue (p= 0.03). Conclusion The method allowed the determination of the glycosaminoglycans structural profile in colorectal tissue from neoplastic and non-neoplastic colorectal tissue. Neoplastic tissues showed greater amounts of chondroitin sulphate and dermatan sulphate compared to non-neoplastic tissues, while heparan sulphate was decreased in neoplastic tissues.
Jornal Brasileiro De Pneumologia Publicacao Oficial Da Sociedade Brasileira De Pneumologia E Tisilogia, Jul 1, 2008
Objetivo: Analisar a taxa de aproveitamento de pulmões disponibilizados em São Paulo no ano de 20... more Objetivo: Analisar a taxa de aproveitamento de pulmões disponibilizados em São Paulo no ano de 2006, bem como caracterizar o perfil dos doadores de pulmão deste período. Métodos: Estudo retrospectivo de 497 prontuários de doadores de pulmão, no período de janeiro a dezembro de 2006. Resultados: Não houve oferta de doação de pulmões para transplante em 149 (30%) dos prontuários analisados, sendo excluídos do estudo. A idade média dos 348 doadores eleitos para o estudo foi de 37,4 ± 16,1 anos, e 56,9% deles eram do sexo masculino. As principais causas da morte cerebral dos doadores foram: acidente vascular cerebral (40,5%); trauma cranioencefálico (34,2%); e hemorragia subaracnóidea (10,9%). A grande maioria dos doadores recebia drogas vasoativas (90,5%), sendo que 13,5% haviam apresentado parada cárdio-respiratória. Do ponto de vista infeccioso, o leucograma médio foi de 15.008 ± 6.467 células/mm 3 , 67,8% recebiam antibioticoterapia e 26,1% apresentavam infecção pulmonar. Quase 40% dos doadores apresentavam alterações radiográficas. Apenas 4,9% dos doadores foram aceitos, representando 28 pulmões (taxa de aproveitamento de órgãos de 4%). Os motivos de recusa foram: alteração gasométrica (30,1%); infecção (23,7%); e distância (10,9%). Conclusões: A taxa de aproveitamento de pulmões em nosso meio é baixa quando comparada às taxas médias de aproveitamento mundial. Além disso, mais de 50% das recusas deveram-se à gasometria arterial inadequada e infecção pulmonar. Aliar melhor cuidado ao potencial doador à menor rigidez nos critérios de seleção poderia aumentar a utilização dos órgãos doados.
Revista Brasileira De Anestesiologia, Dec 1, 2007
... Nara de Cássia Mantovani1, Lúcia Maria Martins Zuliani2, Daniela Tiemi Sano2, Daniel Reis Wai... more ... Nara de Cássia Mantovani1, Lúcia Maria Martins Zuliani2, Daniela Tiemi Sano2, Daniel Reis Waisberg3, Israel Ferreira da Silva, TSA4 Jaques ... Dosis adicionales de atra-curio (1/4 de la dosis inicial) fueron administradas para el mantenimiento de bloqueo neuromuscular y la ...
Revista De Medicina, Dec 6, 2009
Waisberg DR, Galvão RC, Galvão FHF, D'Albuquerque LAC. Transplante intestinal em ratos utilizando... more Waisberg DR, Galvão RC, Galvão FHF, D'Albuquerque LAC. Transplante intestinal em ratos utilizando nova técnica de microanastomose sem sutura com cola e molde tubular (Cuff-Glue). Rev Med (São Paulo). 2009 jul.-dez.;88(3/4):207-18. RESUMO: INTRODUÇÃO: A necessidade de sutura microvascular complexa dificulta a pesquisa do transplante intestinal no rato. A técnica de microanastomose com dispositivo tubular (cuff) é simples, porém, a persistência do dispositivo na anastomose desencadeia reação de corpo estranho e trombose. Neste trabalho descrevemos um modelo de transplante intestinal no rato com nova técnica de anastomose sem sutura com cuff e cola. MÉTODO: O enxerto contém o intestino delgado, colo ascendente e pedículo vascular (conduto aortomesentérico e veia porta). Na extremidade da veia e do coto intestinal, fixa-se o cuff, sobre o qual a parede é evertida. No receptor, realiza-se microanastomose aorto-aórtica manual. O cuff é inserido na veia mesentérica superior e fixado por ligadura circular. A margem proximal é levantada, expondo a camada íntima dos vasos, onde se deposita cola. A margem proximal é abaixada, selando a anastomose. As ligaduras fixando o cuff são retiradas possibilitando a exclusão do cuff da anastomose. Após reperfusão do enxerto, o intestino correspondente do receptor é retirado em bloco e se restabelece o trânsito intestinal utilizando a mesma técnica descrita. RESULTADOS: Observou-se adequada reperfusão do enxerto e boa patência das anastomoses. CONCLUSÃO: O modelo descrito simplifica a realização de microanastomose no transplante intestinal em ratos.
The British journal of surgery, 2015
Although anorectal transplantation is a challenging procedure, it is a promising option for patie... more Although anorectal transplantation is a challenging procedure, it is a promising option for patients who have completely lost anorectal function or in whom it failed to develop, as in congenital malformations. The paucity of animal models with which to test functional outcomes was addressed in this study of anorectal manometry in rats. Wistar rats were assigned randomly to four groups: orthotopic anorectal transplantation, heterotopic transplantation, sham operation, or normal control. Bodyweight and anal pressure were measured immediately before and after operation, and on postoperative days 7 and 14. ANOVA and Tukey's test were used to compare results for bodyweight, anal manometry and length of procedure. Immediately after the procedure, mean(s.d.) anal pressure in the orthotopic group (n = 13) dropped from 31·4(13·1) to 1·6(13·1) cmH2 O (P < 0·001 versus both sham operation (n = 13) and normal control (n = 15)), with partial recovery on postoperative day 7 (14·9(13·9) cmH...
Clinics (São Paulo, Brazil), 2012
This study sought to identify the relationship between fibroblast telomerase expression, myofibro... more This study sought to identify the relationship between fibroblast telomerase expression, myofibroblasts, and telomerase-mediated regulatory signals in idiopathic pulmonary fibrosis. Thirty-four surgical lung biopsies, which had been obtained from patients with idiopathic pulmonary fibrosis and histologically classified as usual interstitial pneumonia, were examined. Immunohistochemistry was used to evaluate fibroblast telomerase expression, myofibroblast α-smooth muscle actin expression and the tissue expression of inter leu kin-4, transforming growth factor-β, and basic fibroblast growth factor. The point-counting technique was used to quantify the expression of these markers in unaffected, collapsed, mural fibrosis, and honeycombing areas. The results were correlated to patient survival. Fibroblast telomerase expression and basic fibroblast growth factor tissue expression were higher in collapsed areas, whereas myofibroblast expression and interleukine-4 tissue expression were hig...
Pediatric Emergency Care, May 1, 2011
Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complica... more Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complication of a neck space infection in children associated with high mortality and morbidity. A 3-year-old boy presented with spontaneous massive epistaxis 45 days after a deep neck space infection caused by a peritonsillar abscess. During nasopharyngeal packing, he evolved with cardiac arrest. Intra-arterial angiography was then performed that revealed a large pseudoaneurysm. Endovascular treatment using detachable balloons achieved complete exclusion of the pseudoaneurysm. The child made an uneventful recovery and was discharged with mild left hemiparesis and no deficit of sensory or cognitive functions. Pseudoaneurysms of the internal carotid artery after a deep neck space infection can be associated with delayed and potentially fatal massive epistaxis. Furthermore, a regional (ie, extranasal) blood vessel should be promptly investigated when there are signs of hypovolemic shock. A high level of suspicion and definitive treatment are essential for successful management of these patients.
Revista Brasileira de Anestesiologia, 2007
... 1. Fisioterapeuta; Encarregada do Serviço de Fisioterapia do Hospital Nossa Senhora de Fátima... more ... 1. Fisioterapeuta; Encarregada do Serviço de Fisioterapia do Hospital Nossa Senhora de Fátima da Sociedade Portuguesa de ... implicará índice menor de complicações decorrentes do tempo menor de perma-nência dos pacientes sob ventilação mecânica e abreviação do ...
Pediatric Emergency Care, 2011
Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complica... more Pseudoaneurysm of the cervical internal carotid artery is a very rare, potentially fatal complication of a neck space infection in children associated with high mortality and morbidity. A 3-year-old boy presented with spontaneous massive epistaxis 45 days after a deep neck space infection caused by a peritonsillar abscess. During nasopharyngeal packing, he evolved with cardiac arrest. Intra-arterial angiography was then performed that revealed a large pseudoaneurysm. Endovascular treatment using detachable balloons achieved complete exclusion of the pseudoaneurysm. The child made an uneventful recovery and was discharged with mild left hemiparesis and no deficit of sensory or cognitive functions. Pseudoaneurysms of the internal carotid artery after a deep neck space infection can be associated with delayed and potentially fatal massive epistaxis. Furthermore, a regional (ie, extranasal) blood vessel should be promptly investigated when there are signs of hypovolemic shock. A high level of suspicion and definitive treatment are essential for successful management of these patients.
Clinics, 2006
A 57-year-old, white woman presented epigastralgia, steatorrhea, and an 8 kg weight loss, with an... more A 57-year-old, white woman presented epigastralgia, steatorrhea, and an 8 kg weight loss, with an onset 1 year previously. Laboratory exams were normal except for the following: (i) presence of anemia (hemoglobin 7.5 g%), (ii) positive test for occult blood in the stool, and (iii) ...
Clinics (São Paulo, Brazil), 2012
We aimed to evaluate whether the inclusion of videothoracoscopy in a pleural empyema treatment al... more We aimed to evaluate whether the inclusion of videothoracoscopy in a pleural empyema treatment algorithm would change the clinical outcome of such patients. This study performed quality-improvement research. We conducted a retrospective review of patients who underwent pleural decortication for pleural empyema at our institution from 2002 to 2008. With the old algorithm (January 2002 to September 2005), open decortication was the procedure of choice, and videothoracoscopy was only performed in certain sporadic mid-stage cases. With the new algorithm (October 2005 to December 2008), videothoracoscopy became the first-line treatment option, whereas open decortication was only performed in patients with a thick pleural peel (>2 cm) observed by chest scan. The patients were divided into an old algorithm (n = 93) and new algorithm (n = 113) group and compared. The main outcome variables assessed included treatment failure (pleural space reintervention or death up to 60 days after medi...
Jornal Vascular Brasileiro, 2010
Mais Serviços Personalizados Artigo texto em Português pdf em Português ReadCube Artigo em XML Re... more Mais Serviços Personalizados Artigo texto em Português pdf em Português ReadCube Artigo em XML Referências do artigo Como citar este artigo Curriculum ScienTI Tradução automática Enviar este artigo por email Indicadores Citado por SciELO Acessos Links relacionados Compartilhar Permalink Jornal Vascular Brasileiro versão impressa ISSN 1677-5449 J. vasc. bras. vol.9 no.1 Porto Alegre 2010 http://dx.
Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia, 2011
To study the functional behavior of the allograft with immunosuppression of pancreatic islets in ... more To study the functional behavior of the allograft with immunosuppression of pancreatic islets in the spleen. Five groups of 10 Mongrel dogs were used: Group A (control) underwent biochemical tests; Group B underwent total pancreatectomy; Group C underwent total pancreatectomy and pancreatic islet autotransplant in the spleen; Group D underwent pancreatic islet allograft in the spleen without immunosuppressive therapy; Group E underwent pancreatic islet allograft in the spleen and immunosuppression with cyclosporine. All of the animals with grafts received pancreatic islets prepared by the mechanical-enzymatic method - stationary collagenase digestion and purification with dextran discontinuous density gradient, implanted in the spleen. The animals with autotransplant and those with allografts with immunosuppression that became normoglycemic showed altered results of intravenous tolerance glucose (p < 0.001) and peripheral and splenic vein plasmatic insulin levels were significant...
Revista Brasileira de Cirurgia Cardiovascular, 2010
Academic Leagues (Academic Interest Group) are entities that belong to medical schools and their ... more Academic Leagues (Academic Interest Group) are entities that belong to medical schools and their hospitals. Their goals are research, medical education and health care. The cardiothoracic Surgery League (Liga de Cirurgia Cardiotorácica--LCCT), University of São Paulo Medical School, was founded 12 years ago, aimed at providing undergraduate medical students an opportunity to expand knowledge in cardiothoracic surgery and to take part in scientific studies in the field. To evaluate the experience gained through LCCT implementation. Current students and former members of LCCT were submitted to a specific evaluation form, delivered personally or by email, which provided data on the general opinion regarding LCCT&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s activities, the reasons for answers provided and choice of medical specialties. Furthermore, LCCT&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s total scientific production was evaluated. Most undergraduate medical students 51/65 (78.5%) and former members 94/121 (77.7%) completed the form. The interest in Cardiothoracic Surgery was the most common reason for joining LCCT 44/161 (27.3%). Most students 32/51 (62.7%)--reported their initial expectations were partially met. Almost every member would recommend a fellow medical student to join LCCT 50/51 (98%) and 25/51 (49%) of them participated in a scientific project linked to LCCT. In total, 47 scientific papers were published having LCCT&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s medical students as authors or co-authors during the existence of the league. Of the former members who chose a surgical career, 7/52 (13.4%) chose thoracic or cardiovascular surgery. LCCT has met its goals, since most current and former members recognized its role in their medical training, besides it is a good way to produce scientific initiation.
Acta Cirurgica Brasileira, 2011
To introduce a model of equipment for inhalation anesthesia in rats that offers better control of... more To introduce a model of equipment for inhalation anesthesia in rats that offers better control of both flow and losses of ether during induction, maintenance, and recuperation. The equipment consists of an air compressor with two outlets, a closed glass induction chamber, a glass reservoir for the anesthetic agent, a pediatric inhalation mask, a three-way stopcock, a Y-connector, and urinary catheters. Three hundred Wistar rats (Rattus norvegicus albinus) were given inhalation anesthesia. The evaluated parameters were equipment operation, duration of each phase of anesthesia, corneal reflex, muscular tonus, respiration during induction and maintenance, and volume of anesthesia. The average time taken for induction was 7.3 minutes; the average anesthetic recuperation time was 6.4 minutes. The amount of anesthetic used varied according to the weight of the animal, with the average volume of ether used being 6.5ml/hour. The availability of oxygen (room air) decreased the recuperation time and averted both respiratory depression and insufficient depth of anesthesia. The proposed equipment is practical, inexpensive, and allows for satisfactory control of anesthetic parameters during the entire procedure, making inhalation anesthesia in rats safe and essentially complication free.
Revista de Medicina, 2008
Junqueira JJM, Waisberg DR, Noleto GS, Samano MN, Pego-Fernandes PM, Jatene FB. Análise das carac... more Junqueira JJM, Waisberg DR, Noleto GS, Samano MN, Pego-Fernandes PM, Jatene FB. Análise das características dos pulmões disponibilizados para transplante no Estado de São Paulo. Rev Med (São Paulo). 2008 jan.-mar.;87(1):44-57. RESUMO: Objetivos: Analisar a taxa de aproveitamento de pulmões disponibilizados em São Paulo em 2006, bem como caracterizar o perfi l dos doadores de pulmão deste período. Métodos: Estudo retrospectivo de 497 prontuários de doadores de pulmão, no período de jan/2006 a dez/2006. Resultados: Dos 497 prontuários analisados, 149 (30%) não tiveram os pulmões doados, sendo excluídos do estudo. Dos 348 doadores eleitos para o estudo, 56,9% eram do sexo masculino, com idade média de 37,4±16,1anos.
Xenotransplantation, 2010
Xenotransplantation and multivisceral transplantation are advanced therapeutic methods that still... more Xenotransplantation and multivisceral transplantation are advanced therapeutic methods that still require a scientific basis. There are no experimental models of multivisceral transplantation available, particularly not the monitoring by endoscopy. Here, we describe the endoscopic features in a model of multivisceral xenotransplantation. The distal esophagus, stomach, intestine, colon, liver, pancreas, and the kidneys with a common vascular pedicle were harvested from rabbits and implanted in swine (group I, n = 9) or in rabbits (group II, n = 4). Endoscopy was performed in the stomach, jejunum, and ascending colon at four consecutive time points (immediate after surgery and 10, 90, and 180 min after reperfusion). Lesions were macroscopically graded as mild, moderate, and severe. Biopsies were taken following sacrifice at 180 min after reperfusion. In group I, the stomach, jejunum, and colon manifested a progression of lesions with predominance of mild lesions after 10 min, mild to moderate lesions after 90 min, and moderate to severe lesions after 180 min. In animals from group II, endoscopy showed normal features at all time points after reperfusion. Histopathologic analysis confirmed the diagnosis of hyperacute rejection in group I. Grafts from group II animals presented normal or mild ischemic/reperfusion injury. All animals subjected to multivisceral xenotransplantation showed a progression of endoscopic lesions with time after transplantation, while animals subjected to allotransplantation showed no aberrations in endoscopy. We conclude that endoscopy is a useful tool in the assessment of hyperacute rejection of a xenograft.