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Papers by Daniel I Zapata
Critical Care Clinics, 2019
When large medical centers become debilitated, patient care needs to be distributed to other func... more When large medical centers become debilitated, patient care needs to be distributed to other functional facilities. "Accommodations must be made for both a disasterrelated surge in patients and the usual intake of patients with unrelated urgent medical and surgical needs." 1
Journal of bronchology & interventional pulmonology, Apr 1, 2018
B48. CRITICAL CARE: MEASURE FOR MEASURE - QUALITY IMPROVEMENT AND IMPLEMENTATION OF BEST PRACTICE, 2019
Toxins, 2013
Smokeless tobacco products have been associated with increased risks of oro-pharyngeal cancers, d... more Smokeless tobacco products have been associated with increased risks of oro-pharyngeal cancers, due in part to the presence of tobacco-specific nitrosamines (TSNAs) such as 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). These potent carcinogens are formed during tobacco curing and as a result of direct nitrosation reactions that occur in the oral cavity. In the current work we describe the isolation and characterization of a hybridoma secreting a high-affinity, NNK-specific monoclonal antibody. A structurally-related benzoyl derivative was synthesized to facilitate coupling to NNK-carrier proteins, which were characterized for the presence of the N-nitroso group using the Griess reaction, and used to immunize BALB/c mice. Splenocytes from mice
Critical Care Medicine, 2016
Critical Care Medicine
Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common ... more Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common practice on the regional medical floors and in intensive care units. Adequately training house staff in the principals of chest tube management is vital given the frequent use of chest tubes. Methods: A questionnaire survey consisting of 17 questions was distributed amongst medical house staff prior to or after group conferences and meetings to assess the level of knowledge, skill and attitude to the fundamentals of chest tube management. The questions utilized in the survey originate from peer-reviewed validated questionnaires and studies. Results: Total of 74% (68/92) of all medicine house staff completed the survey. Among all respondents, 67% (46/68) were not aware of hospital guidelines regarding chest tube management and 54% (37/68) of respondents had never received formal teaching in chest tube management. Of those that received teaching, the modalities were as follows - attendings...
F1000Research, 2015
Kaposi Sarcoma (KS) is an angioproliferative tumor associated with human herpes virus 8 (HHV-8). ... more Kaposi Sarcoma (KS) is an angioproliferative tumor associated with human herpes virus 8 (HHV-8). Often known as one of the acquired immunodeficiency syndrome (AIDS)-defining skin diseases, pulmonary involvement in KS has only been discussed in a handful of case reports, rarely in a non-HIV patient. Herein we report the case of a 77 year-old- male who presented with a 6-week history of progressive dyspnea on exertion accompanied by productive cough of yellow sputum and intermittent hemoptysis. His past medical history was significant for Non-Hodgkin's Follicular B-Cell Lymphoma (NHL). Patient also had biopsy-confirmed cutaneous KS. His physical exam was notable for a 2cm firm, non-tender, mobile right submandibular lymph node. Lungs were clear to auscultation. He had multiple violet non-tender skin lesions localized to the lower extremities. CT scan of the chest showed numerous nodular opacities and small pleural effusions in both lungs. A thoracenthesis was performed, showing sero-sanguineous exudative effusions. Histopathology failed to demonstrate malignant cells or lymphoma. A subsequent bronchoscopy revealed diffusely hyperemic, swollen mucosa of the lower airways with mucopurulent secretions. Bronchoalveolar lavage PCR for HHV-8 showed 5800 DNA copies/mL. It was believed that his pulmonary symptoms were likely due to disseminated KS. This case illustrates the potential for significant lung injury from KS. It also demonstrates the use of PCR for HHV-8 to diagnose KS in a bronchoalveolar lavage sample in a case when bronchoscopic biopsy was not safe. Furthermore, this case is unique in that the patient did not match the typical KS subgroups as HIV infection and other immune disorders were ruled out. Recognition of this syndrome is critical to the institution of appropriate therapy. As such, this case should be of interest to a broad readership across internal medicine including the specialties of Pulmonology and Critical Care.
Cirugía y cirujanos
tuberculosis (TB) is a disease with a high incidence and prevalence worldwide. Renal TB is the se... more tuberculosis (TB) is a disease with a high incidence and prevalence worldwide. Renal TB is the second most common extrapulmonary form of TB. The purpose of this report is to present the case of a patient with renal TB in order to emphasize the importance of this disease. we report the case of a 30-year-old female who presented with fever, cough, diaphoresis and an abdominal right flank mass. Right hydronephrosis, dilated collector system and loss of renal function were documented. A right nephrectomy was performed. Histopathological exam revealed acid-fast mycobacteria, granulomas and multinucleated Langhans-type giant cells. Renal TB was diagnosed and anti-TB treatment was initiated. renal TB is a disease whose incidence has increased in relation to the TB epidemic. Renal TB should be considered in the evaluation of renal masses.
Journal of Strength and Conditioning Research, 2014
The purpose of the study was to compare the effects of progressive volume-based overload to const... more The purpose of the study was to compare the effects of progressive volume-based overload to constant volume-based overload on muscle explosive and endurance performance adaptations during a bi-weekly short-term (i.e. six weeks) plyometric training intervention in young soccer players. Three groups of young soccer players (age 13.0 ± 2.3 y) were divided into: control (CG; n = 8), plyometric training with (PPT; n = 8) and without (NPPT; n = 8) a progressive increase in volume (i.e. 16 jumps per leg/week, with an initial volume of 80 jumps per leg each session). Bilateral and unilateral horizontal and vertical countermovement jump with arms (CMJA), 20 cm drop jump reactive strength index (RSI20), maximal kicking velocity (MKV), 10-m sprint, change of direction speed (CODS) and Yo-Yo intermittent recovery level 1 test (Yo-Yo IR1)
Critical Care Medicine
Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common ... more Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common practice on the regional medical floors and in intensive care units. Adequately training house staff in the principals of chest tube management is vital given the frequent use of chest tubes. Methods: A questionnaire survey consisting of 17 questions was distributed amongst medical house staff prior to or after group conferences and meetings to assess the level of knowledge, skill and attitude to the fundamentals of chest tube management. The questions utilized in the survey originate from peer-reviewed validated questionnaires and studies. Results: Total of 74% (68/92) of all medicine house staff completed the survey. Among all respondents, 67% (46/68) were not aware of hospital guidelines regarding chest tube management and 54% (37/68) of respondents had never received formal teaching in chest tube management. Of those that received teaching, the modalities were as follows - attendings...
Critical Care Clinics, 2019
When large medical centers become debilitated, patient care needs to be distributed to other func... more When large medical centers become debilitated, patient care needs to be distributed to other functional facilities. "Accommodations must be made for both a disasterrelated surge in patients and the usual intake of patients with unrelated urgent medical and surgical needs." 1
Journal of bronchology & interventional pulmonology, Apr 1, 2018
B48. CRITICAL CARE: MEASURE FOR MEASURE - QUALITY IMPROVEMENT AND IMPLEMENTATION OF BEST PRACTICE, 2019
Toxins, 2013
Smokeless tobacco products have been associated with increased risks of oro-pharyngeal cancers, d... more Smokeless tobacco products have been associated with increased risks of oro-pharyngeal cancers, due in part to the presence of tobacco-specific nitrosamines (TSNAs) such as 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). These potent carcinogens are formed during tobacco curing and as a result of direct nitrosation reactions that occur in the oral cavity. In the current work we describe the isolation and characterization of a hybridoma secreting a high-affinity, NNK-specific monoclonal antibody. A structurally-related benzoyl derivative was synthesized to facilitate coupling to NNK-carrier proteins, which were characterized for the presence of the N-nitroso group using the Griess reaction, and used to immunize BALB/c mice. Splenocytes from mice
Critical Care Medicine, 2016
Critical Care Medicine
Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common ... more Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common practice on the regional medical floors and in intensive care units. Adequately training house staff in the principals of chest tube management is vital given the frequent use of chest tubes. Methods: A questionnaire survey consisting of 17 questions was distributed amongst medical house staff prior to or after group conferences and meetings to assess the level of knowledge, skill and attitude to the fundamentals of chest tube management. The questions utilized in the survey originate from peer-reviewed validated questionnaires and studies. Results: Total of 74% (68/92) of all medicine house staff completed the survey. Among all respondents, 67% (46/68) were not aware of hospital guidelines regarding chest tube management and 54% (37/68) of respondents had never received formal teaching in chest tube management. Of those that received teaching, the modalities were as follows - attendings...
F1000Research, 2015
Kaposi Sarcoma (KS) is an angioproliferative tumor associated with human herpes virus 8 (HHV-8). ... more Kaposi Sarcoma (KS) is an angioproliferative tumor associated with human herpes virus 8 (HHV-8). Often known as one of the acquired immunodeficiency syndrome (AIDS)-defining skin diseases, pulmonary involvement in KS has only been discussed in a handful of case reports, rarely in a non-HIV patient. Herein we report the case of a 77 year-old- male who presented with a 6-week history of progressive dyspnea on exertion accompanied by productive cough of yellow sputum and intermittent hemoptysis. His past medical history was significant for Non-Hodgkin's Follicular B-Cell Lymphoma (NHL). Patient also had biopsy-confirmed cutaneous KS. His physical exam was notable for a 2cm firm, non-tender, mobile right submandibular lymph node. Lungs were clear to auscultation. He had multiple violet non-tender skin lesions localized to the lower extremities. CT scan of the chest showed numerous nodular opacities and small pleural effusions in both lungs. A thoracenthesis was performed, showing sero-sanguineous exudative effusions. Histopathology failed to demonstrate malignant cells or lymphoma. A subsequent bronchoscopy revealed diffusely hyperemic, swollen mucosa of the lower airways with mucopurulent secretions. Bronchoalveolar lavage PCR for HHV-8 showed 5800 DNA copies/mL. It was believed that his pulmonary symptoms were likely due to disseminated KS. This case illustrates the potential for significant lung injury from KS. It also demonstrates the use of PCR for HHV-8 to diagnose KS in a bronchoalveolar lavage sample in a case when bronchoscopic biopsy was not safe. Furthermore, this case is unique in that the patient did not match the typical KS subgroups as HIV infection and other immune disorders were ruled out. Recognition of this syndrome is critical to the institution of appropriate therapy. As such, this case should be of interest to a broad readership across internal medicine including the specialties of Pulmonology and Critical Care.
Cirugía y cirujanos
tuberculosis (TB) is a disease with a high incidence and prevalence worldwide. Renal TB is the se... more tuberculosis (TB) is a disease with a high incidence and prevalence worldwide. Renal TB is the second most common extrapulmonary form of TB. The purpose of this report is to present the case of a patient with renal TB in order to emphasize the importance of this disease. we report the case of a 30-year-old female who presented with fever, cough, diaphoresis and an abdominal right flank mass. Right hydronephrosis, dilated collector system and loss of renal function were documented. A right nephrectomy was performed. Histopathological exam revealed acid-fast mycobacteria, granulomas and multinucleated Langhans-type giant cells. Renal TB was diagnosed and anti-TB treatment was initiated. renal TB is a disease whose incidence has increased in relation to the TB epidemic. Renal TB should be considered in the evaluation of renal masses.
Journal of Strength and Conditioning Research, 2014
The purpose of the study was to compare the effects of progressive volume-based overload to const... more The purpose of the study was to compare the effects of progressive volume-based overload to constant volume-based overload on muscle explosive and endurance performance adaptations during a bi-weekly short-term (i.e. six weeks) plyometric training intervention in young soccer players. Three groups of young soccer players (age 13.0 ± 2.3 y) were divided into: control (CG; n = 8), plyometric training with (PPT; n = 8) and without (NPPT; n = 8) a progressive increase in volume (i.e. 16 jumps per leg/week, with an initial volume of 80 jumps per leg each session). Bilateral and unilateral horizontal and vertical countermovement jump with arms (CMJA), 20 cm drop jump reactive strength index (RSI20), maximal kicking velocity (MKV), 10-m sprint, change of direction speed (CODS) and Yo-Yo intermittent recovery level 1 test (Yo-Yo IR1)
Critical Care Medicine
Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common ... more Introduction: The use of chest tubes to evacuate air or fluid from the pleural space is a common practice on the regional medical floors and in intensive care units. Adequately training house staff in the principals of chest tube management is vital given the frequent use of chest tubes. Methods: A questionnaire survey consisting of 17 questions was distributed amongst medical house staff prior to or after group conferences and meetings to assess the level of knowledge, skill and attitude to the fundamentals of chest tube management. The questions utilized in the survey originate from peer-reviewed validated questionnaires and studies. Results: Total of 74% (68/92) of all medicine house staff completed the survey. Among all respondents, 67% (46/68) were not aware of hospital guidelines regarding chest tube management and 54% (37/68) of respondents had never received formal teaching in chest tube management. Of those that received teaching, the modalities were as follows - attendings...