Daniela Marroquín - Academia.edu (original) (raw)

Papers by Daniela Marroquín

Research paper thumbnail of DOI:10.5897/AJB11.3702 ISSN 1684–5315 © 2012 Academic Journals

Changes in the protein profile of Habanero pepper (Capsicum chinense J.) somatic embryos during d... more Changes in the protein profile of Habanero pepper (Capsicum chinense J.) somatic embryos during development

Research paper thumbnail of Ontogenesis of the Somatic Embryogenesis of Habanero Pepper (Capsicum chinense Jacq.)

HortScience, 2009

The ontogenesis of direct high-frequency somatic embryogenesis of C. chinense induced from hypoco... more The ontogenesis of direct high-frequency somatic embryogenesis of C. chinense induced from hypocotyl was characterized through a histological analysis of the different phases in the histodifferentiation process during the development of the somatic embryo. The anatomical analysis was carried out since the hypocotyl segments were placed in the culture medium until 45 days of culture. The somatic embryos were induced and maintained in Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (9.5 μm). Samples of tissues and organs were taken every 24 h, fixed in formalin acetic alcohol, and embedded in plastic resin. They were cut into serial sections (5 μm) and stained with toluidine blue. The analysis revealed that the proembryogenic cells originated just from provascular hypocotyl cells. Provascular cells acquired the embryogenic competence 48 h after induction and an intense mitotic division was observed and embryogenic structures were generated first along the v...

Research paper thumbnail of Capsaicinoids Content in Habanero Pepper (Capsicum chinense Jacq.): Hottest Known Cultivars

HortScience, 2008

The aim of this study was to determine the pungency level of different accessions of Habanero pep... more The aim of this study was to determine the pungency level of different accessions of Habanero peppers. The high-performance liquid chromatography (HPLC) technique was used to evaluate the content of total capsaicinoids in the whole fruit, placenta, and pericarp of 18 accessions of Habanero pepper from the germplasm bank of the Capsicum chinense species maintained in the Scientific Research Center of Yucatan [Centro de Investigación Científica de Yucatán (CICY)]. Thirteen of these accessions belonged to the “orange type”, four to the “red type”, and one to the “yellow type”. During the study, the plants were cultivated and maintained under greenhouse conditions and the fruit was harvested only when it was completely ripe on the plant. The results show considerable intraspecific diversity for this characteristic as well as the existence of cultivars of this species that surpass the levels of pungency reported for Habanero peppers under the conditions evaluated.

Research paper thumbnail of In Vitro Selection of a Salt-tolerant Embryogenic Line in Habanero Pepper (Capsicum chinense Jacq.)

HortScience, 2011

The effect of NaCI salinity on growth and development of somatic embryos of Habanero pepper was e... more The effect of NaCI salinity on growth and development of somatic embryos of Habanero pepper was examined. Addition of 75 and 100 mm NaCI into the medium greatly increased the growth and development of somatic embryos and both of these concentrations favored the proliferation of somatic embryos. However, supplementation of 200 and 300 mm NaCI to the medium showed a negative effect on the growth and development of somatic embryos. Concentration increases of NaCl provoked a significant reduction of the embryos survival rate with the average lethal dose (46%) being registered in the treatment of 100 mm. Furthermore, a lower tolerance to salt stress (NaCl) was observed in deformed somatic embryos. Concentrations of 200 and 300 mm NaCl significantly delayed development in the surviving embryos in both treatments. These embryos remained at the globular stage throughout culture time. At 75 mm NaCl, most of the embryos were observed in the torpedo stage. However, the embryos exposed to 100 m...

Research paper thumbnail of Tissue culture of Capsicum species

Peppers: botany, production and uses

Research paper thumbnail of Changes in the protein profile of Habanero pepper (Capsicum chinense J.) somatic embryos during development

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2012

Protein profile was studied during the development of Capsicum chinense somatic embryos. The tota... more Protein profile was studied during the development of Capsicum chinense somatic embryos. The total protein content and profile of polypeptides (by sodium dodecyl sulfate polyacrylamide gel electrophoresis) of somatic embryos at different developmental stages (globular, heart-shaped, torpedo and cotyledonary stages) were analyzed. The protein profile of zygotic embryos included nine exclusive bands with molecular weights of 4

Research paper thumbnail of DOI:10.5897/AJB11.3702 ISSN 1684–5315 © 2012 Academic Journals

Changes in the protein profile of Habanero pepper (Capsicum chinense J.) somatic embryos during d... more Changes in the protein profile of Habanero pepper (Capsicum chinense J.) somatic embryos during development

Research paper thumbnail of Ontogenesis of the Somatic Embryogenesis of Habanero Pepper (Capsicum chinense Jacq.)

HortScience, 2009

The ontogenesis of direct high-frequency somatic embryogenesis of C. chinense induced from hypoco... more The ontogenesis of direct high-frequency somatic embryogenesis of C. chinense induced from hypocotyl was characterized through a histological analysis of the different phases in the histodifferentiation process during the development of the somatic embryo. The anatomical analysis was carried out since the hypocotyl segments were placed in the culture medium until 45 days of culture. The somatic embryos were induced and maintained in Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (9.5 μm). Samples of tissues and organs were taken every 24 h, fixed in formalin acetic alcohol, and embedded in plastic resin. They were cut into serial sections (5 μm) and stained with toluidine blue. The analysis revealed that the proembryogenic cells originated just from provascular hypocotyl cells. Provascular cells acquired the embryogenic competence 48 h after induction and an intense mitotic division was observed and embryogenic structures were generated first along the v...

Research paper thumbnail of Capsaicinoids Content in Habanero Pepper (Capsicum chinense Jacq.): Hottest Known Cultivars

HortScience, 2008

The aim of this study was to determine the pungency level of different accessions of Habanero pep... more The aim of this study was to determine the pungency level of different accessions of Habanero peppers. The high-performance liquid chromatography (HPLC) technique was used to evaluate the content of total capsaicinoids in the whole fruit, placenta, and pericarp of 18 accessions of Habanero pepper from the germplasm bank of the Capsicum chinense species maintained in the Scientific Research Center of Yucatan [Centro de Investigación Científica de Yucatán (CICY)]. Thirteen of these accessions belonged to the “orange type”, four to the “red type”, and one to the “yellow type”. During the study, the plants were cultivated and maintained under greenhouse conditions and the fruit was harvested only when it was completely ripe on the plant. The results show considerable intraspecific diversity for this characteristic as well as the existence of cultivars of this species that surpass the levels of pungency reported for Habanero peppers under the conditions evaluated.

Research paper thumbnail of In Vitro Selection of a Salt-tolerant Embryogenic Line in Habanero Pepper (Capsicum chinense Jacq.)

HortScience, 2011

The effect of NaCI salinity on growth and development of somatic embryos of Habanero pepper was e... more The effect of NaCI salinity on growth and development of somatic embryos of Habanero pepper was examined. Addition of 75 and 100 mm NaCI into the medium greatly increased the growth and development of somatic embryos and both of these concentrations favored the proliferation of somatic embryos. However, supplementation of 200 and 300 mm NaCI to the medium showed a negative effect on the growth and development of somatic embryos. Concentration increases of NaCl provoked a significant reduction of the embryos survival rate with the average lethal dose (46%) being registered in the treatment of 100 mm. Furthermore, a lower tolerance to salt stress (NaCl) was observed in deformed somatic embryos. Concentrations of 200 and 300 mm NaCl significantly delayed development in the surviving embryos in both treatments. These embryos remained at the globular stage throughout culture time. At 75 mm NaCl, most of the embryos were observed in the torpedo stage. However, the embryos exposed to 100 m...

Research paper thumbnail of Tissue culture of Capsicum species

Peppers: botany, production and uses

Research paper thumbnail of Changes in the protein profile of Habanero pepper (Capsicum chinense J.) somatic embryos during development

AFRICAN JOURNAL OF BIOTECHNOLOGY, 2012

Protein profile was studied during the development of Capsicum chinense somatic embryos. The tota... more Protein profile was studied during the development of Capsicum chinense somatic embryos. The total protein content and profile of polypeptides (by sodium dodecyl sulfate polyacrylamide gel electrophoresis) of somatic embryos at different developmental stages (globular, heart-shaped, torpedo and cotyledonary stages) were analyzed. The protein profile of zygotic embryos included nine exclusive bands with molecular weights of 4