Danny Halim - Academia.edu (original) (raw)

Papers by Danny Halim

Journal of Neurodevelopmental Disorders

Background Subtle abnormalities in children’s intelligence, motor skills, and psychology from var... more Background Subtle abnormalities in children’s intelligence, motor skills, and psychology from various assisted reproductive treatments (ARTs) might be underdiagnosed. Understanding the prognosis of intelligence, motor skills, and psychology in children from ART would provide parents with reasonable expectations and enable them to plan relevant support to achieve the optimum potential in ART children. Methods We searched PubMed, EMBASE, Ovid, Google Scholar, and Scopus databases until April 13, 2021, to identify relevant studies. Thirty-four studies met the inclusion and exclusion criteria. The meta-analysis employed a standardized mean difference model. The outcome of this study is to compare intelligence quotient (IQ), motoric ability, and behavioral problems between all ARTs, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) to naturally conceived (NC) children. Subdomains of intelligence based on the Cattell, Horn, and Carroll Model (CHC Model) of cognitive ar...

Erlangga, 2010

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Pediatric Vascular Neurosurgery, 2021

Human Molecular Genetics

Filamin A (FLNA) is a cytoplasmic actin binding protein, recently shown to be expressed as a long... more Filamin A (FLNA) is a cytoplasmic actin binding protein, recently shown to be expressed as a long and short isoform. Mutations in FLNA are associated with a wide spectrum of disorders, including an X-linked form of chronic intestinal pseudo-obstruction (CIPO). However, the role of FLNA in intestinal development and function is largely unknown. In this study, we show that FLNA is expressed in the muscle layer of the small intestine from early human fetal stages. Expression of FLNA variants associated with CIPO, blocked expression of the long flna isoform and led to an overall reduction of RNA and protein levels. As a consequence, contractility of human intestinal smooth muscle cells was affected. Lastly, our transgenic zebrafish line showed that the flna long isoform is required for intestinal elongation and peristalsis. Histological analysis revealed structural and architectural changes in the intestinal smooth muscle of homozygous fish, likely triggered by the abnormal expression o...

Indonesian Journal of Cancer, Oct 1, 2010

Open Access Macedonian Journal of Medical Sciences, 2020

BACKGROUND: Patients who are diagnosed with trisomy 13 (Patau syndrome) are known to have a poor ... more BACKGROUND: Patients who are diagnosed with trisomy 13 (Patau syndrome) are known to have a poor prognosis. It has been hypothesized that such poor outcomes are suspected to be attributed to their central nervous system (CNS)-malformations and cardiac-malformations. This study was conducted at Division of Neuropediatric, Department of Neurosurgery, Faculty of Medicine, Universitas Padjadjaran – Dr. Hasan Sadikin Hospital, Bandung (2012–2018). AIM: This study aimed to describe clinical characteristics and karyotype findings in patients who were diagnosed with Patau syndrome and treated at our center. Since Indonesia is still categorized as a lower middle-income country with limited resources, we expected that this study would provide a clinical reference on how a congenital disease with chromosomal abnormalities is confirmed. CASE PRESENTATION: Our cases indicate that CNS malformations are likely to be the cause of indirect mortality of patients’ early period of life. The median surv...

Supplemental Table 1. Newcastle Ottawa Scale on Cohort Studies. Supplemental Table 2. Newcastle-O... more Supplemental Table 1. Newcastle Ottawa Scale on Cohort Studies. Supplemental Table 2. Newcastle-Ottawa Scale on Case-Control Studies. Supplemental Table 3. Characteristics of the study. Supplemental Table 4. Statistical Summary of Meta-Analysis. Supplemental Table 5. Summary of findings and study quality assessment with GRADE approach of ART vs Non-ART studies. Supplemental Table 6. Summary of findings and study quality assessment with GRADE approach of ICSI vs IVF studies. Supplemental Table 7. Summary of findings and study quality assessment with GRADE approach of Frozen vs Fresh Embryo Transfer studies. Supplemental Table 8. Summary of findings and study quality assessment with GRADE approach of Confounder Effects studies. Supplemental Table 9. Summary of findings and study quality assessment with GRADE approach of Risk of Multiple Birth, Preterm Birth, Low birth body weight in ART and Non-ART children.

Regenerative Engineering and Translational Medicine, 2021

The COVID-19 disease, which is caused by the novel coronavirus, SARS-CoV-2, has affected the worl... more The COVID-19 disease, which is caused by the novel coronavirus, SARS-CoV-2, has affected the world by increasing the mortality rate in 2020. Currently, there is no definite treatment for COVID-19 patients. Several clinical trials have been proposed to overcome this disease and many are still under investigation. In this review, we will be focusing on the potency of mesenchymal stem cells (MSCs) and MSC-derived secretome for treating COVID-19 patients. Fever, cough, headache, dizziness, and fatigue are the common clinical manifestations in COVID-19 patients. In mild and severe cases, cytokines are released hyperactively which causes a cytokine storm leading to acute respiratory distress syndrome (ARDS). In order to maintain the lung microenvironment in COVID-19 patients, MSCs are used as cell-based therapy approaches as they can act as cell managers which accelerate the immune system to prevent the cytokine storm and promote endogenous repair. Besides, MSCs have shown minimal expression of ACE2 or TMPRSS2, and hence, MSCs are free from SARS-CoV-2 infection. Numerous clinical studies have started worldwide and demonstrated that MSCs have great potential for ARDS treatment in COVID-19 patients. Preliminary data have shown that MSCs and MSC-derived secretome appear to be promising in the treatment of COVID-19. Lay Summary The COVID-19 disease is an infection disease which affects the world in 2020. Currently, there is no definite treatment for COVID-19 patients. However, several clinical trials have been proposed to overcome this disease and one of them is using mesenchymal stem cells (MSCs) and MSC-derived secretome for treating COVID-19 patients. During the infection, cytokines are released hyper-actively which causes a cytokine storm. MSCs play an important role in maintaining the lung microenvironment in COVID-19 patients. They can act as cell managers which accelerate the immune system to prevent the cytokine storm and promote the endogenous repair. Therefore, it is important to explore the clinical trial in the world for treating the COVID-19 disease using MSCs and MSC-derived secretome.

Child's Nervous System, 2021

Hydrocephalus is diagnosed when an accumulating amount of cerebrospinal fluid (CSF) fails to circ... more Hydrocephalus is diagnosed when an accumulating amount of cerebrospinal fluid (CSF) fails to circulate and/or absorbed in the ventricular system. Based on its etiology, hydrocephalus can be classified into infectious and non-infectious hydrocephalus. In children, non-infectious hydrocephalus includes congenital hydrocephalus, posthemorrhagic hydrocephalus, neural tube defect–related hydrocephalus, and tumor-related hydrocephalus. Regardless of the cause, a CSF diversion device is placed to divert the excess fluid from the ventricles into peritoneal cavity. Among all, ventriculoperitoneal (VP) shunt is arguably the most commonly used CSF diversion device to date. Until now, the long-term neurodevelopmental impact of VP shunt placement in non-infectious hydrocephalus patients remained unclear. This study aims to evaluate the neurodevelopmental outcomes in children with non-infectious hydrocephalus who had VP shunt placement. Systematic searches were performed using PubMed, Google Scholar, Scopus databases, and reference lists. Publications that fulfilled the inclusion criteria were included in the meta-analysis. Calculation of Mantel-Haezel risk ratio (RR) was applied, and heterogeneity index (I2) test was used to evaluate the existence of heterogeneity in all studies. Risk of bias was assessed based on the criteria from the Newcastle-Ottawa Scale (NOS). Of the 1929 studies identified, 12 publications were concluded to have fulfilled the inclusion criteria. Results from the meta-analysis showed that the risks of cerebral palsy, visual and hearing impairment, epilepsy, or seizures are significantly higher in children with non-infectious hydrocephalus who already had VP shunt placement (shunted non-infectious hydrocephalus, S-NIH) compared to that of the healthy control. The meta-analysis on intelligent quotient (IQ) and mental development index (MDI) showed that S-NIH children tend to score lower IQ and acquire risk of having mental development delay. On motoric development, S-NIH children scored lower motoric score and have significantly higher risk of motor development delay compared to control. Although normal children tend to have more internalizing behavior compared to S-NIH children, overall assessment on the risk of behavioral abnormalities showed that the differences between these two groups are insignificant. S-NIH children have significantly higher risks of disabilities and mental and motoric development delays; thus, planning on continuous rehabilitation for children with non-infectious hydrocephalus who already had placement of VP shunt is important to acquire their optimum potentials and quality of life.

markdownabstractAll research described in this dissertation is focused on understanding the patho... more markdownabstractAll research described in this dissertation is focused on understanding the pathophysiology of three rare congenital diseases of the intestine, including megacystis microcolon intestinal hypoperistalsis syndrome (MMIHS), congenital short bowel syndrome (CSBS), and hereditary multiple intestinal atresia (HMIA). For MMIHS and HMIA, we follow a comparable path, starting a search for the genetic cause of the disease by SNP arrays and exome sequencing. Subsequently, after identifying the candidate genes, we performed both in vitro and in vivo assays to prove causality and to determine the mechanism underlying the disease development. For CSBS, we performed functional work to unravel disease mechanism.

The conventional method of mononuclear cells (MNCs) isolation from human umbilical cord blood (UC... more The conventional method of mononuclear cells (MNCs) isolation from human umbilical cord blood (UCB) yielded high erythrocyte contamination level. Therefore, the aim of this study was to assess the differences of cell viability and erythrocyte contamination on the population of UCB MNCs in our modified isolation method. This study was also aimed to test the function and characteristic of human MNCs derived from UCB as the basis for the development of UCB banking in Indonesia. The study was conducted in Department of Obstetry and Ginecology RS Dr. Hasan Sadikin Bandung in period of January–October 2010. The modified isolation method (namely Unpad Aster’s modification) yielded 5.1x106 MNC cell/mL has lower erythrocyte contamination level than conventional method. The morphology of MNCs cultured in unrestricted somatic stem cells (USSCs) medium looked like adhered cells (attached at the surface of culture flask), spindle-shaped cells with positive luster of ifferentiation-90 (CD-90) (le...

BACKGROUND: Gliomas are the most common primary brain tumors, representing 50–60% of malignant pr... more BACKGROUND: Gliomas are the most common primary brain tumors, representing 50–60% of malignant primary brain tumors. Gliomas are highly heterogeneous with marked inter- and intratumoral diversity. Gliomas heterogeneity is a challenging issue in the development of personalized treatment. The simplest method for studying heterogeneity is using ex vivo cell cultures; in our case, the cell lines were isolated from patient with glioblastomas. AIM: Here, we reported distinct cell subpopulations heterogeneity in glioblastoma cells. METHODS: Human glioblastoma cells isolation is conducted by enzymatic method with combination of collagenase I, hyaluronidase, and trypsin enzyme in proportional amount from patient. Immunostaining was performed to assess glial fibrillary acidic protein (GFAP), Ki-67, isocitrate dehydrogenase-1 (IDH-1) status, and program death ligand-1 (PD-L1) expression. Primary glioblastoma cell line was characterized by flow cytometry (fluorescence-activated cell sorting) an...

OBJECTIVE This study aimed to evaluate the developmental outcomes in children from cryopreserved ... more OBJECTIVE This study aimed to evaluate the developmental outcomes in children from cryopreserved embryos, with minimum influences of interparental variation that would cause potential bias. Hence we examined siblings, in which the older sibs were from fresh embryo transfers, while the younger sibs were from cryopreserved embryos. METHODS Three pairs of siblings were evaluated. All routine prenatal and neonatal evaluation were performed, while personal-social, language, fine and gross motor evaluation were all evaluated by the Denver Developmental Screening Test (DDST)-II. Wechsler Preschool and Primary Scale of Intelligence (WPPSI) test was used to measure the Intelligent Quotient (IQ) in 5 of 6 children. RESULTS Standard prenatal measurements of all children suggested uneventful pregnancies, followed by uneventful deliveries. DDST-II results showed that the aspects of personal-social, language, fine and gross motor in every child are as expected according to their ages. Results fro...

International Journal of PharmTech Research, 2020

Introduction: Glioblastoma is the deadliest malignant brain tumors in adults. The main challenges... more Introduction: Glioblastoma is the deadliest malignant brain tumors in adults. The main challenges in treating glioblastoma are its resistance to the chemo-radiotherapy, poor outcome and low survival rate. The World Health Organization (WHO) 2016 classification identifies two types of glioblastoma by its mutational status of isocitrate dehydrogenase (IDH); since our national insurance experiences budget-limitation, we could not freely apply it in our institutions. We aims to find the prevalence and outcome of glioblastoma-not otherwise specified (NOS) based on its clinical manifestations and histopathology findings. Methods: We performed retrograde-analysis based on clinical and histology findings on 48 glioblastoma-NOS patients from 2012-2017. We analyzed its characteristic, primary complains, lesions location, macroscopic findings, therapy and the final outcomes. Results: Glioblastoma-NOS is the most common type of gliomas occurs in adults ages 49.29±12.13 years (range 17-72 years)...

Interdisciplinary Neurosurgery, 2020

Coronavirus Infectious Disease 2019 (COVID-19) caused by severe acute respiratory syndrome corona... more Coronavirus Infectious Disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; previously known as 2019 novel coronavirus) is an emerging and rapidly evolving health issue that has been widespread globally and become a pandemic. The typical symptoms of COVID-19 are: a cough, shortness of breath and a fever; from the initial estimates, about 15% of COVID-19 patients present with severe respiratory symptoms and requires hospitalization and intensive care. Recent accumulated evidences showed that the neurological insults also occurred in patients with COVID-19, ranging from mild headache to severe neurological symptoms. In this review, we summarize the COVID-19 and neurological significance of COVID-19.

Majalah Kedokteran Bandung, 2010

Metode konvensional isolasi sel endotel pembuluh darah otak (EPDO) masih tergolong sulit, sehingg... more Metode konvensional isolasi sel endotel pembuluh darah otak (EPDO) masih tergolong sulit, sehingga upaya mendapatkan populasi murni sel ini adalah tantangan. Pada penelitian ini dilakukan isolasi endotel dari tikus Wistar dan mencit C57/Bl6, berdasarkan protokol the care and use of laboratory animals, Universitas Gunma, Jepang. Modifikasi metode isolasi adalah menggunakan gradasi bovine serum albumin (BSA), bukan Dextran-70 yang umumnya dipakai, untuk memisahkan sel EPDO yang bersatu menjadi sel EPDO tunggal. Penelitian ini dilakukan di laboratorium sel kultur, Universitas Padjadjaran bekerjasama dengan Universitas Gunma, Jepang, Januari 2008-Juni 2009. Uji hasil isolasi dan karakteristik sel EPDO dilakukan dengan teknik imunofloresen. Ekspresi tight junction ZO-1, menunjukkan sel EPDO membentuk selapis sel utuh, rapat, tidak bertumpuk dan kompak, sesuai dengan karakteristik dinding EPDO. Fenotip sel EPDO dikonfirmasi dengan acethylated LDL, faktor von Willebrand dan CD31. Penghancuran kapiler dengan collagenase/dispase masih menghasilkan populasi sel yang terkontaminasi perisit. Kontaminasi dimurnikan dengan menggunakan puromycin, tingkat pemurnian sel EPDO mencapai 98,3%. Simpulan, teknik modifikasi berhasil mengisolasi sel EPDO tikus dan mencit, tanpa melakukan intervensi genetik.

Cancer Science, 2009

the purpose of this study was to evaluate the prognostic value of l-type amino acid transporter 1... more the purpose of this study was to evaluate the prognostic value of l-type amino acid transporter 1 (lat1) and 4F2 heavy chain (cd98) expression in patients with stage iii non-small cell lung cancer (nSclc). a total of 188 consecutive patients with pathologic stage iii nSclc were retrospectively reviewed. the expression of lat1, cd98, Ki-67 labeling index, vascular endothelial growth factor (VEGF) as well as microvessel density (mVd) were evaluated immunohistochemically and correlated with the prognosis of patients after complete resection of the tumor. positive expression of lat1 and cd98 was noted in 58% (109/188) and 50% (94/188) of the cases, respectively (p=0.1473). a positive rate of LAT1 expression was significantly higher in squamous cell carcinoma (SQc) (90%, 48/53) and large-cell carcinoma (lcc) (100%, 12/12) than in adenocarcinoma (ac) (40%, 49/123). moreover, a positive rate of lat1 with CD98 expression was also significantly higher in SQC (74%, 39/53) and lcc (75%, 9/12) than ac (34%, 42/123). lat1 expression was significantly higher in patients with mediastinal lymph node metastases than in patients without, and was significantly correlated with CD98, Ki-67 labeling index, VEGF and mVd. the 5-year survival rates of lat1-positive and-negative patients and cd98-positive and-negative patients were 27.9 and 40.6% (p=0.0033), respectively, and 24.1 and 43.6% (p=0.0004), respectively. multivariate analysis confirmed that positive expression of LAT1 and CD98 was an independent factor predicting a poor prognosis. in conclusion, the overexpression of lat1 and cd98 is a pathological factor for predicting the prognosis of patients with surgically resectable stage iii nSclc.