David Myers - Academia.edu (original) (raw)

Papers by David Myers

This report details findings from an experimental evaluation of four widely used programs for ele... more This report details findings from an experimental evaluation of four widely used programs for elementary school students with reading problems—Corrective Reading, Failure Free Reading, Spell Read P.A.T., and Wilson Reading. Conducted just outside Pittsburgh, Pennsylvania, in the Allegheny Intermediate Unit, the evaluation explored the extent to which the programs affect both word-level reading and reading comprehension skills of students in

Scientific Reports, 2015

Investigating biophysical cellular interactions in the circulation currently requires choosing be... more Investigating biophysical cellular interactions in the circulation currently requires choosing between in vivo models, which are difficult to interpret due in part to the hemodynamic and geometric complexities of the vasculature; or in vitro systems, which suffer from non-physiologic assumptions and/or require specialized microfabrication facilities and expertise. To bridge that gap, we developed an in vitro "do-it-yourself" perfusable vasculature model that recapitulates in vivo geometries, such as aneurysms, stenoses, and bifurcations, and supports endothelial cell culture. These inexpensive, disposable devices can be created rapidly (<2 hours) with high precision and repeatability, using standard off-the-shelf laboratory supplies. Using these "endothelialized" systems, we demonstrate that spatial variation in vascular cell adhesion molecule (VCAM-1) expression correlates with the wall shear stress patterns of vascular geometries. We further observe that the presence of endothelial cells in stenoses reduces platelet adhesion but increases sickle cell disease (SCD) red blood cell (RBC) adhesion in bifurcations. Overall, our method enables researchers from all disciplines to study cellular interactions in physiologically relevant, yet simple-to-make, in vitro vasculature models.

PloS one, 2015

During vascular injury, platelets adhere to exposed subendothelial proteins, such as collagen, on... more During vascular injury, platelets adhere to exposed subendothelial proteins, such as collagen, on the blood vessel walls to trigger clot formation. Although the biochemical signalings of platelet-collagen interactions have been well characterized, little is known about the role microenvironmental biomechanical properties, such as vascular wall stiffness, may have on clot formation. To that end, we investigated how substrates of varying stiffness conjugated with the same concentration of Type I collagen affect platelet adhesion, spreading, and activation. Using collagen-conjugated polyacrylamide (PA) gels of different stiffnesses, we observed that platelets do in fact mechanotransduce the stiffness cues of collagen substrates, manifesting in increased platelet spreading on stiffer substrates. In addition, increasing substrate stiffness also increases phosphatidylserine exposure, a key aspect of platelet activation that initiates coagulation on the platelet surface. Mechanistically, t...

Low-income students and students whose parents have not attended college typically are less likel... more Low-income students and students whose parents have not attended college typically are less likely than middle- and upper-income students to complete high school and attend college, and are thus less likely to reap the benefits of attending college. In 1965, Congress established the Talent Search Program to provide information on the high school courses students should take to prepare for

Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science, 2003

Previous studies from our institution have shown that ventilated caging run at negative pressure ... more Previous studies from our institution have shown that ventilated caging run at negative pressure to a mouse room dramatically reduced exposure of personnel to the major mouse allergen, Mus m 1. The current study was designed to determine whether negative cage ventilation posed an inordinate risk for spread of infectious agents between cages and/or racks. B6;129S-Tnfsf5(tm1Imx)/J (TNF) mice, which were naturally and persistently infected with Pneumocystis carinii, Helicobacter bilis, and Pasteurella pneumotropica, were used as the source of infections. Uninfected C3Smn.CB17-Prkdc(scid)/J (SCID) mice with severe combined immunodeficiency were used to detect transmission. The following methods were used to detect transmission of infections: polymerase chain reaction (PCR) amplification and histological examination of lungs for P. carinii; PCR of fecal specimens or cecal contents for H. bilis; and culture of oropharyngeal, tracheal, or vaginal swabs for P. pneumotropica. We determined w...

Upward Bound is one of the largest and longest running federal programs designed to help economic... more Upward Bound is one of the largest and longest running federal programs designed to help economically disadvantaged students go to college. This report from Mathematica's national evaluation finds that Upward Bound increased the number of high school math credits that participants earned but did not affect other measures of academic preparation in high school. The program also increased enrollment at

We present poly-SiC coating and subsequent operation of a Si-based double-ended tuning fork (DETF... more We present poly-SiC coating and subsequent operation of a Si-based double-ended tuning fork (DETF) resonant strain sensor fabricated in the Bosch commercial foundry process. The coating is applied post release and, hence, has minimal impact on the front end of the microfabrication process. The deposition thickness of nanometer-thin SiC coating was optimized to provide enhanced corrosion resistance to silicon MEMS

Biomicrofluidics, 2014

Cell culture in microfluidic systems has primarily been conducted in devices comprised of polydim... more Cell culture in microfluidic systems has primarily been conducted in devices comprised of polydimethylsiloxane (PDMS) or other elastomers. As polystyrene (PS) is the most characterized and commonly used substrate material for cell culture, microfluidic cell culture would ideally be conducted in PS-based microsystems that also enable tight control of perfusion and hydrodynamic conditions, which are especially important for culture of vascular cell types. Here, we report a simple method to prototype perfusable PS microfluidics for endothelial cell culture under flow that can be fabricated using standard lithography and wet laboratory equipment to enable stable perfusion at shear stresses up to 300 dyn/cm(2) and pumping pressures up to 26 kPa for at least 100 h. This technique can also be extended to fabricate perfusable hybrid PS-PDMS microfluidics of which one application is for increased efficiency of viral transduction in non-adherent suspension cells by leveraging the high surface area to volume ratio of microfluidics and adhesion molecules that are optimized for PS substrates. These biologically compatible microfluidic devices can be made more accessible to biological-based laboratories through the outsourcing of lithography to various available microfluidic foundries.

Proceedings of the National Academy of Sciences, 2014

As platelets aggregate and activate at the site of vascular injury to stem bleeding, they are sub... more As platelets aggregate and activate at the site of vascular injury to stem bleeding, they are subjected to a myriad of biochemical and biophysical signals and cues. As clot formation ensues, platelets interact with polymerizing fibrin scaffolds, exposing platelets to a large range of mechanical microenvironments. Here, we show for the first time (to our knowledge) that platelets, which are anucleate cellular fragments, sense microenvironmental mechanical properties, such as substrate stiffness, and transduce those cues into differential biological signals. Specifically, as platelets mechanosense the stiffness of the underlying fibrin/fibrinogen substrate, increasing substrate stiffness leads to increased platelet adhesion and spreading. Importantly, adhesion on stiffer substrates also leads to higher levels of platelet activation, as measured by integrin αIIbβ3 activation, α-granule secretion, and procoagulant activity. Mechanistically, we determined that Rac1 and actomyosin activity mediate substrate stiffness-dependent platelet adhesion, spreading, and activation to different degrees. This capability of platelets to mechanosense microenvironmental cues in a growing thrombus or hemostatic plug and then mechanotransduce those cues into differential levels of platelet adhesion, spreading, and activation provides biophysical insight into the underlying mechanisms of platelet aggregation and platelet activation heterogeneity during thrombus formation.

Journal of School Health, 1992

The changing context of teen-age childbearing and current related controversies are reviewed. Rec... more The changing context of teen-age childbearing and current related controversies are reviewed. Recent research about the consequences of teen-age childbearing for the teen-age mother, the father, and for the children born is examined. The article also summarizes current knowledge about the consequences of teen-age childbearing with regard to the mother's educational attainment, marital experience, subsequent fertility behavior, labor force experience and occupational attainment, and experience with poverty and welfare.

This first European edition of Social Psychology builds on the factually rigorous and intellectua... more This first European edition of Social Psychology builds on the factually rigorous and intellectually provocative work of the original US book to offer a comprehensive discussion of one of the most captivating, dynamic and popular areas of Psychology. Thoroughly revised and updated to include the most up to date research from Europe, North America as well as more International research, this book serves to help us answer many fundamental and intriguing questions about our selves and those who surround us, including: How does thinking ' both conscious and unconscious ' drive our behaviour? What leads people sometimes to hurt and sometimes to help one another? What fosters group unity, cohesion and mutual influence? This book will help you study this exciting discipline and help you find answers to these and other questions. This text has been written for the student, to be accessible and interesting, helping them to expand their understanding and motivate them to enquire and...

Psychological Bulletin, 1989

APA PsycNET Our Apologies! - The following features are not available with your current Browser c... more APA PsycNET Our Apologies! - The following features are not available with your current Browser configuration. - alerts user that their session is about to expire - display, print, save, export, and email selected records - get My ...

Animal Learning & Behavior, 1983

Page 1. Animal Learning & Behavior 1983.11 (1),107-115 Conditioned flavor prefere... more Page 1. Animal Learning & Behavior 1983.11 (1),107-115 Conditioned flavor preferences based on hunger level during original flavor exposure ELIZABETH D. CAPALDI, DAVID E. MYERS DAVID H. CAMPBELL, and JOAN ...

Laboratory Animals, 2001

Our purpose in this investigation was to determ ine if we could reduce cage changing frequency wi... more Our purpose in this investigation was to determ ine if we could reduce cage changing frequency without adversely affec ting the health of mice. We housed mice at three different cage changing frequencies: 7, 14, and 21 days, each at three different cage ventilat ion rates: 30, 60 and 100 air changes per hour (ACH), for a total of nine experimental conditions. For each condition, we evaluated the health of 12 breeding pairs and 12 breeding trios of C57BL = 6J mice for 7 months. Health was assessed by breeding performance, weanling weight and growth, plasma corticosterone levels, immune function, and histological examinat ion of selected organs. Over a period of 4 months, we monitored the cage microenvironment for ammonia and carbon dioxide concentrations, relative humidity, and temperature one day prior to changing the cage. T he relati ve humidity, carbon dioxide concentrations, and temperature of the cages at all conditions were within acceptable levels. Ammonia concentrations remained below 25 ppm (parts per million) in most cages, but, even at higher concentrations, did not adversely affec t the health of mice. Frequency of cage changing had only one signi®cant effect; pup mortal ity with pair mat ings was greater at the cage changing frequency of 7 days compared with 14 or 21 days. In additi on, pup mortality with pair matings was higher at 30 ACH compared with other ventilat ion rates. In conclusion, under the conditions of this study, cage changes once every 14 days and ventilation rates of 60 ACH provide optim um conditions for anim al health and practical husbandry.

Journal of the American Academy of Dermatology, 2012

Journal of Research on Adolescence, 1993

Page 1. JOURNAL OF RESEARCH ON ADOLESCENCE, 3(4), 393-422 Copyright 0 1993, Lawrence Erlbaum Asso... more Page 1. JOURNAL OF RESEARCH ON ADOLESCENCE, 3(4), 393-422 Copyright 0 1993, Lawrence Erlbaum Associates, Inc. Age at First Childbirth and Later Poverty Kristin Anderson Moore Child Trends, Inc. David E. Myers ...

Journal of Personality and Social Psychology, 1971

... Appreciation is expressed to Neil Blahut for his assistance in collecting the data. 2 Request... more ... Appreciation is expressed to Neil Blahut for his assistance in collecting the data. 2 Requests for reprints should be sent to David Myers, Department of Psychology, Hope College, Holland, Michigan 49423. 3 Now at Yale University. supposed. ...

This report details findings from an experimental evaluation of four widely used programs for ele... more This report details findings from an experimental evaluation of four widely used programs for elementary school students with reading problems—Corrective Reading, Failure Free Reading, Spell Read P.A.T., and Wilson Reading. Conducted just outside Pittsburgh, Pennsylvania, in the Allegheny Intermediate Unit, the evaluation explored the extent to which the programs affect both word-level reading and reading comprehension skills of students in

Scientific Reports, 2015

Investigating biophysical cellular interactions in the circulation currently requires choosing be... more Investigating biophysical cellular interactions in the circulation currently requires choosing between in vivo models, which are difficult to interpret due in part to the hemodynamic and geometric complexities of the vasculature; or in vitro systems, which suffer from non-physiologic assumptions and/or require specialized microfabrication facilities and expertise. To bridge that gap, we developed an in vitro "do-it-yourself" perfusable vasculature model that recapitulates in vivo geometries, such as aneurysms, stenoses, and bifurcations, and supports endothelial cell culture. These inexpensive, disposable devices can be created rapidly (<2 hours) with high precision and repeatability, using standard off-the-shelf laboratory supplies. Using these "endothelialized" systems, we demonstrate that spatial variation in vascular cell adhesion molecule (VCAM-1) expression correlates with the wall shear stress patterns of vascular geometries. We further observe that the presence of endothelial cells in stenoses reduces platelet adhesion but increases sickle cell disease (SCD) red blood cell (RBC) adhesion in bifurcations. Overall, our method enables researchers from all disciplines to study cellular interactions in physiologically relevant, yet simple-to-make, in vitro vasculature models.

PloS one, 2015

During vascular injury, platelets adhere to exposed subendothelial proteins, such as collagen, on... more During vascular injury, platelets adhere to exposed subendothelial proteins, such as collagen, on the blood vessel walls to trigger clot formation. Although the biochemical signalings of platelet-collagen interactions have been well characterized, little is known about the role microenvironmental biomechanical properties, such as vascular wall stiffness, may have on clot formation. To that end, we investigated how substrates of varying stiffness conjugated with the same concentration of Type I collagen affect platelet adhesion, spreading, and activation. Using collagen-conjugated polyacrylamide (PA) gels of different stiffnesses, we observed that platelets do in fact mechanotransduce the stiffness cues of collagen substrates, manifesting in increased platelet spreading on stiffer substrates. In addition, increasing substrate stiffness also increases phosphatidylserine exposure, a key aspect of platelet activation that initiates coagulation on the platelet surface. Mechanistically, t...

Low-income students and students whose parents have not attended college typically are less likel... more Low-income students and students whose parents have not attended college typically are less likely than middle- and upper-income students to complete high school and attend college, and are thus less likely to reap the benefits of attending college. In 1965, Congress established the Talent Search Program to provide information on the high school courses students should take to prepare for

Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science, 2003

Previous studies from our institution have shown that ventilated caging run at negative pressure ... more Previous studies from our institution have shown that ventilated caging run at negative pressure to a mouse room dramatically reduced exposure of personnel to the major mouse allergen, Mus m 1. The current study was designed to determine whether negative cage ventilation posed an inordinate risk for spread of infectious agents between cages and/or racks. B6;129S-Tnfsf5(tm1Imx)/J (TNF) mice, which were naturally and persistently infected with Pneumocystis carinii, Helicobacter bilis, and Pasteurella pneumotropica, were used as the source of infections. Uninfected C3Smn.CB17-Prkdc(scid)/J (SCID) mice with severe combined immunodeficiency were used to detect transmission. The following methods were used to detect transmission of infections: polymerase chain reaction (PCR) amplification and histological examination of lungs for P. carinii; PCR of fecal specimens or cecal contents for H. bilis; and culture of oropharyngeal, tracheal, or vaginal swabs for P. pneumotropica. We determined w...

Upward Bound is one of the largest and longest running federal programs designed to help economic... more Upward Bound is one of the largest and longest running federal programs designed to help economically disadvantaged students go to college. This report from Mathematica's national evaluation finds that Upward Bound increased the number of high school math credits that participants earned but did not affect other measures of academic preparation in high school. The program also increased enrollment at

We present poly-SiC coating and subsequent operation of a Si-based double-ended tuning fork (DETF... more We present poly-SiC coating and subsequent operation of a Si-based double-ended tuning fork (DETF) resonant strain sensor fabricated in the Bosch commercial foundry process. The coating is applied post release and, hence, has minimal impact on the front end of the microfabrication process. The deposition thickness of nanometer-thin SiC coating was optimized to provide enhanced corrosion resistance to silicon MEMS

Biomicrofluidics, 2014

Cell culture in microfluidic systems has primarily been conducted in devices comprised of polydim... more Cell culture in microfluidic systems has primarily been conducted in devices comprised of polydimethylsiloxane (PDMS) or other elastomers. As polystyrene (PS) is the most characterized and commonly used substrate material for cell culture, microfluidic cell culture would ideally be conducted in PS-based microsystems that also enable tight control of perfusion and hydrodynamic conditions, which are especially important for culture of vascular cell types. Here, we report a simple method to prototype perfusable PS microfluidics for endothelial cell culture under flow that can be fabricated using standard lithography and wet laboratory equipment to enable stable perfusion at shear stresses up to 300 dyn/cm(2) and pumping pressures up to 26 kPa for at least 100 h. This technique can also be extended to fabricate perfusable hybrid PS-PDMS microfluidics of which one application is for increased efficiency of viral transduction in non-adherent suspension cells by leveraging the high surface area to volume ratio of microfluidics and adhesion molecules that are optimized for PS substrates. These biologically compatible microfluidic devices can be made more accessible to biological-based laboratories through the outsourcing of lithography to various available microfluidic foundries.

Proceedings of the National Academy of Sciences, 2014

As platelets aggregate and activate at the site of vascular injury to stem bleeding, they are sub... more As platelets aggregate and activate at the site of vascular injury to stem bleeding, they are subjected to a myriad of biochemical and biophysical signals and cues. As clot formation ensues, platelets interact with polymerizing fibrin scaffolds, exposing platelets to a large range of mechanical microenvironments. Here, we show for the first time (to our knowledge) that platelets, which are anucleate cellular fragments, sense microenvironmental mechanical properties, such as substrate stiffness, and transduce those cues into differential biological signals. Specifically, as platelets mechanosense the stiffness of the underlying fibrin/fibrinogen substrate, increasing substrate stiffness leads to increased platelet adhesion and spreading. Importantly, adhesion on stiffer substrates also leads to higher levels of platelet activation, as measured by integrin αIIbβ3 activation, α-granule secretion, and procoagulant activity. Mechanistically, we determined that Rac1 and actomyosin activity mediate substrate stiffness-dependent platelet adhesion, spreading, and activation to different degrees. This capability of platelets to mechanosense microenvironmental cues in a growing thrombus or hemostatic plug and then mechanotransduce those cues into differential levels of platelet adhesion, spreading, and activation provides biophysical insight into the underlying mechanisms of platelet aggregation and platelet activation heterogeneity during thrombus formation.

Journal of School Health, 1992

The changing context of teen-age childbearing and current related controversies are reviewed. Rec... more The changing context of teen-age childbearing and current related controversies are reviewed. Recent research about the consequences of teen-age childbearing for the teen-age mother, the father, and for the children born is examined. The article also summarizes current knowledge about the consequences of teen-age childbearing with regard to the mother's educational attainment, marital experience, subsequent fertility behavior, labor force experience and occupational attainment, and experience with poverty and welfare.

This first European edition of Social Psychology builds on the factually rigorous and intellectua... more This first European edition of Social Psychology builds on the factually rigorous and intellectually provocative work of the original US book to offer a comprehensive discussion of one of the most captivating, dynamic and popular areas of Psychology. Thoroughly revised and updated to include the most up to date research from Europe, North America as well as more International research, this book serves to help us answer many fundamental and intriguing questions about our selves and those who surround us, including: How does thinking ' both conscious and unconscious ' drive our behaviour? What leads people sometimes to hurt and sometimes to help one another? What fosters group unity, cohesion and mutual influence? This book will help you study this exciting discipline and help you find answers to these and other questions. This text has been written for the student, to be accessible and interesting, helping them to expand their understanding and motivate them to enquire and...

Psychological Bulletin, 1989

APA PsycNET Our Apologies! - The following features are not available with your current Browser c... more APA PsycNET Our Apologies! - The following features are not available with your current Browser configuration. - alerts user that their session is about to expire - display, print, save, export, and email selected records - get My ...

Animal Learning & Behavior, 1983

Page 1. Animal Learning & Behavior 1983.11 (1),107-115 Conditioned flavor prefere... more Page 1. Animal Learning & Behavior 1983.11 (1),107-115 Conditioned flavor preferences based on hunger level during original flavor exposure ELIZABETH D. CAPALDI, DAVID E. MYERS DAVID H. CAMPBELL, and JOAN ...

Laboratory Animals, 2001

Our purpose in this investigation was to determ ine if we could reduce cage changing frequency wi... more Our purpose in this investigation was to determ ine if we could reduce cage changing frequency without adversely affec ting the health of mice. We housed mice at three different cage changing frequencies: 7, 14, and 21 days, each at three different cage ventilat ion rates: 30, 60 and 100 air changes per hour (ACH), for a total of nine experimental conditions. For each condition, we evaluated the health of 12 breeding pairs and 12 breeding trios of C57BL = 6J mice for 7 months. Health was assessed by breeding performance, weanling weight and growth, plasma corticosterone levels, immune function, and histological examinat ion of selected organs. Over a period of 4 months, we monitored the cage microenvironment for ammonia and carbon dioxide concentrations, relative humidity, and temperature one day prior to changing the cage. T he relati ve humidity, carbon dioxide concentrations, and temperature of the cages at all conditions were within acceptable levels. Ammonia concentrations remained below 25 ppm (parts per million) in most cages, but, even at higher concentrations, did not adversely affec t the health of mice. Frequency of cage changing had only one signi®cant effect; pup mortal ity with pair mat ings was greater at the cage changing frequency of 7 days compared with 14 or 21 days. In additi on, pup mortality with pair matings was higher at 30 ACH compared with other ventilat ion rates. In conclusion, under the conditions of this study, cage changes once every 14 days and ventilation rates of 60 ACH provide optim um conditions for anim al health and practical husbandry.

Journal of the American Academy of Dermatology, 2012

Journal of Research on Adolescence, 1993

Page 1. JOURNAL OF RESEARCH ON ADOLESCENCE, 3(4), 393-422 Copyright 0 1993, Lawrence Erlbaum Asso... more Page 1. JOURNAL OF RESEARCH ON ADOLESCENCE, 3(4), 393-422 Copyright 0 1993, Lawrence Erlbaum Associates, Inc. Age at First Childbirth and Later Poverty Kristin Anderson Moore Child Trends, Inc. David E. Myers ...

Journal of Personality and Social Psychology, 1971

... Appreciation is expressed to Neil Blahut for his assistance in collecting the data. 2 Request... more ... Appreciation is expressed to Neil Blahut for his assistance in collecting the data. 2 Requests for reprints should be sent to David Myers, Department of Psychology, Hope College, Holland, Michigan 49423. 3 Now at Yale University. supposed. ...