David Williams - Academia.edu (original) (raw)

Papers by David Williams

Electrochimica Acta, 2011

... Permissions & Reprints. The topography and morphology of low shields and associated l... more ... Permissions & Reprints. The topography and morphology of low shields and associated landforms of plains volcanism in the Tharsis region of Mars. ... Icelandic shields, distinct structures in Hawai'i, and other basaltic landforms also show similarities to Martian plains volcanism. ...

Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 1991

Nuclear Instruments and Methods in Physics Research A307 (1991) 47-51 47 North-Holland Particle i... more Nuclear Instruments and Methods in Physics Research A307 (1991) 47-51 47 North-Holland Particle identification by Cherenkov ring imaging using a neural network approach Tom Francke and Thomas Lindblad Manne Siegbahn Institute of Physics, Stockholm, Sweden Age ...

Neurobiology of Aging, 2004

Mineralium Deposita, 1968

Journal of Veterinary Internal Medicine, 1999

Proteinase inhibitors are proteins in the body that regulate the catalytic activity of proteinase... more Proteinase inhibitors are proteins in the body that regulate the catalytic activity of proteinases. They are important in a large variety of physiologic processes including coagulation, digestion, tumor metastasis and immunity. Proteinase inhibitors are categorized as either nonspecific proteinase inhibitors or class-specific proteinase inhibitors. Nonspecific proteinase inhibitors are comprised soley of the alpha macroglobulins, most notably alpha2-macroglobulin. Class-specific proteinase inhibitors are subcategorized as serine proteinase inhibitors, aspartic proteinase inhibitors, metalloproteinase inhibitors, and cysteine proteinase inhibitors. Each subcategory is made up of numerous inhibitors. As the roles of individual proteinase inhibitors are determined, the therapeutic use of natural and synthetic proteinase inhibitors is also being investigated. The purpose of this article is to review the history and classification of proteinase inhibitors and their relevance to veterinary medicine.

Journal of Veterinary Internal Medicine, 2004

Journal of Magnetic Resonance Imaging, 1999

Journal of Geophysical Research, 2005

[2] Variable features, first seen on Mariner 9 images, were defined by Sagan et al.[1972] for alb... more [2] Variable features, first seen on Mariner 9 images, were defined by Sagan et al.[1972] for albedo patterns that change with time. They are considered to represent sand (62.5–2000 mm), silt (4–62.5 mm), and dust (< 4 mm) particles redistributed by winds. As such, ...

The Journal of Clinical Hypertension, 2001

physica status solidi (c), 2012

Catheterization and Cardiovascular Interventions, 2007

The impact of chronic coronary obstructions on resting blood flow in stable cardiac patients and ... more The impact of chronic coronary obstructions on resting blood flow in stable cardiac patients and the response to percutaneous coronary intervention (PCI) using the TIMI frame count method has not been well documented. We studied the impact of coronary artery stenosis severity on the corrected TIMI frame count (cTFC) in chronically stenosed coronary arteries. We prospectively and quantitatively determined the impact of stenting on the cTFC during elective PCI. In substudy 1, analysis was performed to obtain the mean cTFC for arteries with <50% stenosis (Group A), 51-75% stenosis (Group B), 76-85% stenosis (Group C1), 86-95% stenosis (Group C2) and 96-99% stenosis (Group C3). In substudy 2, the cTFC and quantitative coronary angiography were performed pre- and post-PCI. In substudy 1, the cTFC increased exponentially beyond a diameter stenosis of 75% (P < 0.01). However there was no significant difference in the cTFC for coronary arteries with <75% stenosis. In substudy 2, the overall pre- and poststenting cTFC was 17.1 +/- 11.7 and 7.8 +/- 2.7 (P < 0.01) and the TFC index [calculated by dividing the mean cTFC for the relevant artery by the mean cTFC for the corresponding coronary artery in a previously derived control group in our laboratory] was 1.6 +/- 1 and 0.7 +/- 0.2 (P =…

Cancer, 1989

The compatibility and stability at room temperature for up to 7 days of a three-drug admixture of... more The compatibility and stability at room temperature for up to 7 days of a three-drug admixture of cyclophosphamide, methotrexate, and 5-fluorouracil (5-FU) (CMF) was established permitting the practical delivery of the combination as an infusion in an ambulatory setting. Fourteen patients received 20 courses of CMF administered on a continuous infusion schedule for 14 days of a 28-day cycle. The dose rates were fixed for 5-FU (300 mg/M2/day) and methotrexate (0.75 mg/M2/day). The cyclophosphamide dose was escalated from 25 to 50, 75, and 100 mg/M2/d. Leukopenia and thrombocytopenia were observed in two of five patients receiving the maximal dose of cyclophosphamide. No other toxicities were observed including alopecia, stomatitis or liver function abnormalities. This Phase I trial suggests that the cumulative doses of cyclophosphamide, methotrexate, and 5-FU are comparable to the maximum doses delivered as single agent infusions. Furthermore, when the infusion CMF is compared to the "standard" bolus schedule for CMF, the infusion schedule delivers 116%, 8%, and 350% of the respective three component drugs (cyclophosphamide, methotrexate, and 5-FU).

Biotechnology and Bioengineering, 1981

Biotechnology and Bioengineering, 2009

Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread... more Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2012

Recent evidence suggests that the macrophage scavenger receptor class A (SR-A, aka, CD204) plays ... more Recent evidence suggests that the macrophage scavenger receptor class A (SR-A, aka, CD204) plays a role in the induction of innate immune and inflammatory responses. We investigated whether SR-A will cooperate with Toll-like receptors (TLRs) in response to TLR ligand stimulation. Macrophages (J774/a) were treated with Pam2CSK4, (TLR2 ligand), Polyinosinic:polycytidylic acid (Poly I:C) (TLR3 ligand), and Lipopolysaccharides (LPS) (TLR4 ligand) for 15 min in the presence or absence of fucoidan (the SR-A ligand). The levels of phosphorylated IκBα (p-IκBα) were examined by Western blot. We observed that Poly I:C and LPS alone, but not Pam2CSK4 or fucoidan increased the levels of p-IκBα. However, LPS-induced increases in p-IκBα levels were further enhanced when presence of the fucoidan. Immunoprecipitation and double fluorescent staining showed that LPS stimulation promotes SR-A association with TLR4 in the presence of fucoidan. To further confirm our observation, we isolated peritoneal macrophages from SR-A deficient (SR-A(-/-)), TLR4(-/-) and wild type (WT) mice, respectively. The peritoneal macrophages were treated with LPS for 15min in the presence and absence of fucoidan. We observed that LPS-stimulated TNFα and IL-1β production was further enhanced in the WT macrophages, but did not in either TLR4(-/-) or SR-A(-/-) macrophages, when fucoidan was present. Similarly, in the presence of fucoidan, LPS-induced IκBα phosphorylation, NF-κB binding activity, and association between TLR4 and SR-A were significantly enhanced in WT macrophages compared with LPS stimulation alone. The data suggests that SR-A is needed for LPS-induced inflammatory responses in macrophages.

Applied Biochemistry and Biotechnology, 2001

Electrochimica Acta, 2011

... Permissions & Reprints. The topography and morphology of low shields and associated l... more ... Permissions & Reprints. The topography and morphology of low shields and associated landforms of plains volcanism in the Tharsis region of Mars. ... Icelandic shields, distinct structures in Hawai'i, and other basaltic landforms also show similarities to Martian plains volcanism. ...

Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 1991

Nuclear Instruments and Methods in Physics Research A307 (1991) 47-51 47 North-Holland Particle i... more Nuclear Instruments and Methods in Physics Research A307 (1991) 47-51 47 North-Holland Particle identification by Cherenkov ring imaging using a neural network approach Tom Francke and Thomas Lindblad Manne Siegbahn Institute of Physics, Stockholm, Sweden Age ...

Neurobiology of Aging, 2004

Mineralium Deposita, 1968

Journal of Veterinary Internal Medicine, 1999

Proteinase inhibitors are proteins in the body that regulate the catalytic activity of proteinase... more Proteinase inhibitors are proteins in the body that regulate the catalytic activity of proteinases. They are important in a large variety of physiologic processes including coagulation, digestion, tumor metastasis and immunity. Proteinase inhibitors are categorized as either nonspecific proteinase inhibitors or class-specific proteinase inhibitors. Nonspecific proteinase inhibitors are comprised soley of the alpha macroglobulins, most notably alpha2-macroglobulin. Class-specific proteinase inhibitors are subcategorized as serine proteinase inhibitors, aspartic proteinase inhibitors, metalloproteinase inhibitors, and cysteine proteinase inhibitors. Each subcategory is made up of numerous inhibitors. As the roles of individual proteinase inhibitors are determined, the therapeutic use of natural and synthetic proteinase inhibitors is also being investigated. The purpose of this article is to review the history and classification of proteinase inhibitors and their relevance to veterinary medicine.

Journal of Veterinary Internal Medicine, 2004

Journal of Magnetic Resonance Imaging, 1999

Journal of Geophysical Research, 2005

[2] Variable features, first seen on Mariner 9 images, were defined by Sagan et al.[1972] for alb... more [2] Variable features, first seen on Mariner 9 images, were defined by Sagan et al.[1972] for albedo patterns that change with time. They are considered to represent sand (62.5–2000 mm), silt (4–62.5 mm), and dust (< 4 mm) particles redistributed by winds. As such, ...

The Journal of Clinical Hypertension, 2001

physica status solidi (c), 2012

Catheterization and Cardiovascular Interventions, 2007

The impact of chronic coronary obstructions on resting blood flow in stable cardiac patients and ... more The impact of chronic coronary obstructions on resting blood flow in stable cardiac patients and the response to percutaneous coronary intervention (PCI) using the TIMI frame count method has not been well documented. We studied the impact of coronary artery stenosis severity on the corrected TIMI frame count (cTFC) in chronically stenosed coronary arteries. We prospectively and quantitatively determined the impact of stenting on the cTFC during elective PCI. In substudy 1, analysis was performed to obtain the mean cTFC for arteries with <50% stenosis (Group A), 51-75% stenosis (Group B), 76-85% stenosis (Group C1), 86-95% stenosis (Group C2) and 96-99% stenosis (Group C3). In substudy 2, the cTFC and quantitative coronary angiography were performed pre- and post-PCI. In substudy 1, the cTFC increased exponentially beyond a diameter stenosis of 75% (P < 0.01). However there was no significant difference in the cTFC for coronary arteries with <75% stenosis. In substudy 2, the overall pre- and poststenting cTFC was 17.1 +/- 11.7 and 7.8 +/- 2.7 (P < 0.01) and the TFC index [calculated by dividing the mean cTFC for the relevant artery by the mean cTFC for the corresponding coronary artery in a previously derived control group in our laboratory] was 1.6 +/- 1 and 0.7 +/- 0.2 (P =…

Cancer, 1989

The compatibility and stability at room temperature for up to 7 days of a three-drug admixture of... more The compatibility and stability at room temperature for up to 7 days of a three-drug admixture of cyclophosphamide, methotrexate, and 5-fluorouracil (5-FU) (CMF) was established permitting the practical delivery of the combination as an infusion in an ambulatory setting. Fourteen patients received 20 courses of CMF administered on a continuous infusion schedule for 14 days of a 28-day cycle. The dose rates were fixed for 5-FU (300 mg/M2/day) and methotrexate (0.75 mg/M2/day). The cyclophosphamide dose was escalated from 25 to 50, 75, and 100 mg/M2/d. Leukopenia and thrombocytopenia were observed in two of five patients receiving the maximal dose of cyclophosphamide. No other toxicities were observed including alopecia, stomatitis or liver function abnormalities. This Phase I trial suggests that the cumulative doses of cyclophosphamide, methotrexate, and 5-FU are comparable to the maximum doses delivered as single agent infusions. Furthermore, when the infusion CMF is compared to the "standard" bolus schedule for CMF, the infusion schedule delivers 116%, 8%, and 350% of the respective three component drugs (cyclophosphamide, methotrexate, and 5-FU).

Biotechnology and Bioengineering, 1981

Biotechnology and Bioengineering, 2009

Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread... more Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2012

Recent evidence suggests that the macrophage scavenger receptor class A (SR-A, aka, CD204) plays ... more Recent evidence suggests that the macrophage scavenger receptor class A (SR-A, aka, CD204) plays a role in the induction of innate immune and inflammatory responses. We investigated whether SR-A will cooperate with Toll-like receptors (TLRs) in response to TLR ligand stimulation. Macrophages (J774/a) were treated with Pam2CSK4, (TLR2 ligand), Polyinosinic:polycytidylic acid (Poly I:C) (TLR3 ligand), and Lipopolysaccharides (LPS) (TLR4 ligand) for 15 min in the presence or absence of fucoidan (the SR-A ligand). The levels of phosphorylated IκBα (p-IκBα) were examined by Western blot. We observed that Poly I:C and LPS alone, but not Pam2CSK4 or fucoidan increased the levels of p-IκBα. However, LPS-induced increases in p-IκBα levels were further enhanced when presence of the fucoidan. Immunoprecipitation and double fluorescent staining showed that LPS stimulation promotes SR-A association with TLR4 in the presence of fucoidan. To further confirm our observation, we isolated peritoneal macrophages from SR-A deficient (SR-A(-/-)), TLR4(-/-) and wild type (WT) mice, respectively. The peritoneal macrophages were treated with LPS for 15min in the presence and absence of fucoidan. We observed that LPS-stimulated TNFα and IL-1β production was further enhanced in the WT macrophages, but did not in either TLR4(-/-) or SR-A(-/-) macrophages, when fucoidan was present. Similarly, in the presence of fucoidan, LPS-induced IκBα phosphorylation, NF-κB binding activity, and association between TLR4 and SR-A were significantly enhanced in WT macrophages compared with LPS stimulation alone. The data suggests that SR-A is needed for LPS-induced inflammatory responses in macrophages.

Applied Biochemistry and Biotechnology, 2001

Unsaturated soil mechanics continues to play poor relation to saturated soil mechanics, although ... more Unsaturated soil mechanics continues to play poor relation to saturated soil mechanics, although an unsaturated soil at a given density is stronger, less compressible and less permeable (i.e. performs better) than the same soil in a saturated state. There are many examples of unsaturated conditions in the mining field, including the wetting-up and drain-down of initially dry surface waste rock dumps; the irrigation and drain-down of heap leach materials; the drain-down, desiccation and rewetting of mine tailings; the dewatering of mineral products such as coal; the shear strength and compressibility of stored mine wastes; and the performance of geo-covers placed on mine wastes on rehabilitation. This paper highlights the key unsaturated soil mechanics parameters, overviews the nature of mining and processing wastes, and some products, and discusses the issues involved. Some applications of unsaturated soil mechanics addressing the shear strength, compressibility and permeability of mine wastes, and mineral products, are presented, together with data to highlight them.

Unsaturated soil mechanics continues to play poor relation to saturated soil mechanics, although ... more Unsaturated soil mechanics continues to play poor relation to saturated soil mechanics, although an unsaturated soil at a given density is stronger, less compressible and less permeable (i.e. performs better) than the same soil in a saturated state. There are many examples of unsaturated conditions in the mining field, including the wetting-up and drain-down of initially dry surface waste rock dumps; the irrigation and drain-down of heap leach materials; the drain-down, desiccation and rewetting of mine tailings; the dewatering of mineral products such as coal; the shear strength and compressibility of stored mine wastes; and the performance of geo-covers placed on mine wastes on rehabilitation. This paper highlights the key unsaturated soil mechanics parameters, overviews the nature of mining and processing wastes, and some products, and discusses the issues involved. Some applications of unsaturated soil mechanics addressing the shear strength, compressibility and permeability of mine wastes, and mineral products, are presented, together with data to highlight them.