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Debabrata Dash

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Research paper thumbnail of The association of pp125FAK, pp60Src, CDC42Hs and Rap1B with the cytoskeleton of aggregated platelets is a reversible process regulated by calcium

FEBS Letters, 1995

The integrin etnb/33-mediated redistribution of the tyrosine kinases pp125 rAg and pp60 src and t... more The integrin etnb/33-mediated redistribution of the tyrosine kinases pp125 rAg and pp60 src and the small GTP-binding proteins CDC42Hs and RaplB from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 rain) induced by the thrombin-receptor activating peptide (TRAP) these signalling proteins dissociated from the cytoskeleton and reappeared in the membrane skeleton. Addition of the extracellular Ca 2+ chelator EGTA and the intracellular Ca 2+ chelator BAPTA/AM 30 s after TRAP allowed platelet aggregation and the association of pp125 rAK, pp60 s'c, CDC42Hs and RaplB with the cytoskeleton, but prevented their dissociation from the cytoskeleton. The results indicate that the prolonged elevation of cytosolic Ca 2+ in stimulated platelets leads to the dissociation of signalling proteins from the eytoskeleton.

Research paper thumbnail of The association of pp125FAK, pp60Src, CDC42Hs and Rap1B with the cytoskeleton of aggregated platelets is a reversible process regulated by calcium

FEBS Letters, 1995

The integrin etnb/33-mediated redistribution of the tyrosine kinases pp125 rAg and pp60 src and t... more The integrin etnb/33-mediated redistribution of the tyrosine kinases pp125 rAg and pp60 src and the small GTP-binding proteins CDC42Hs and RaplB from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 rain) induced by the thrombin-receptor activating peptide (TRAP) these signalling proteins dissociated from the cytoskeleton and reappeared in the membrane skeleton. Addition of the extracellular Ca 2+ chelator EGTA and the intracellular Ca 2+ chelator BAPTA/AM 30 s after TRAP allowed platelet aggregation and the association of pp125 rAK, pp60 s'c, CDC42Hs and RaplB with the cytoskeleton, but prevented their dissociation from the cytoskeleton. The results indicate that the prolonged elevation of cytosolic Ca 2+ in stimulated platelets leads to the dissociation of signalling proteins from the eytoskeleton.

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