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Papers by Deeba Kamil

The Indian Journal of Agricultural Sciences

In the present study 30 gladiolus genotypes were evaluated for different traits and reaction to t... more In the present study 30 gladiolus genotypes were evaluated for different traits and reaction to two virulent strains of Fusarium, viz. IARI isolate (F. oxysporum f. sp. gladioli â€“ Fog isolate 1) and Pantnagar isolate (F. oxysporum f. sp. gladioli - Fog isolate 2). The two healthy uniform sized corms were planted in individual pots (12â€) filled with potting mixture (soil: FYM: sand; 2:1:1) in five replications. Before planting, the corms were treated with suspension of Fusarium (3 Ã— 106/ml) for 24 hr. Among the genotypes, Dhanwantri (14.59%), Pusa Unnati (14.59%), Swarnima (16.54%), and hybrid Smokey Lady Ã— Oscar (22.92%) were found tolerant to both the Fusarium isolates. The maximum plant height, spike length, rachis length and mean number of florets/spike were noted in Pusa Unnati, but the earliest spike emergence took place in Jyotsana (87.37 days), earliest opening of first floret was noted in Melody (103.79 days). The maximum mean corm weight (87.58 g) was noted in Jyotsan...

PLOS ONE

A desirable substitute for chemical pesticides is mycopesticides. In the current investigation, r... more A desirable substitute for chemical pesticides is mycopesticides. In the current investigation, rDNA-ITS (Internal transcribed spacer) and TEF (Transcriptional Elongation Factor) sequencing were used for molecular identification of six Beauveria bassiana strains. Both, leaf discs and potted plant bioassaye were carried out to study their pathogenicity against the cassava mite, Tetranychus truncatus. LC50 and LC90 values of potential B. bassiana strains were estimated. We also discovered a correlation between intraspecific B. bassiana strains pathogenicity and comprehensive metabolome profiles. Bb5, Bb6, Bb8, Bb12, Bb15, and Bb21 strains were identified as B. bassiana by sequencing of rDNA-ITS and TEF segments and sequence comparison to NCBI (National Center for Biotechnology Information) GenBank. Out of the six strains tested for pathogenicity, Bb6, Bb12, and Bb15 strains outperformed against T. truncatus with LC50 values 1.4×106, 1.7×106, and 1.4×106 and with a LC90 values 7.3×107,...

Frontiers in Microbiology

Beauveria bassiana is an entomopathogenic fungus that causes the white muscadine disease in insec... more Beauveria bassiana is an entomopathogenic fungus that causes the white muscadine disease in insects. The majority of entomopathogenic fungi are soil and insect borne, 15 soil samples were collected from seven different locations during 2021, from January to December. Similarly, during 2022, March to December, 15 fungus-infected insect specimens were collected from five different locations hence soil and insect samples from various ecosystems were collected. As a result, 30 B. bassiana isolates from 11 different geographical areas were identified using morphological characteristics and multilocus sequence data in this investigation. The taxonomical positions of the isolates were determined using morphological characteristics and phylogenetic inferences based on three loci (Internal Transcribed Sequence, Elongation Factor-1α, and B. bassiana chitinase 1). In phylogenetic analysis of B. bassiana, the Maximum Likelihood analytical method produced distinct tree topology when compared to ...

FIGURE 1. Phylogenetic tree based on maximum parsimony analysis with 1000 replications of the tef... more FIGURE 1. Phylogenetic tree based on maximum parsimony analysis with 1000 replications of the tef-1dataset.

FIGURE 6. Trichoderma brevicompactum (A) Growth on PDA, (B) Pustules, (C) Reverse of the plate, (... more FIGURE 6. Trichoderma brevicompactum (A) Growth on PDA, (B) Pustules, (C) Reverse of the plate, (D,E,F) Conidiophore branching, (G) Phialide disposition, (H) Spores, (I) Chlamydospores.

FIGURE 7. Trichoderma pubescens (A) Growth on PDA, (B) Pustules, (C) Reverse of the plate, (D)Ste... more FIGURE 7. Trichoderma pubescens (A) Growth on PDA, (B) Pustules, (C) Reverse of the plate, (D)Sterile hairs extending beyond pustules, (E,F,G)Conidiophores consisting of a sterile elongation with phialides clustered at the base, (H) Phialide disposition, (I) Spores.

7. Trichoderma erinaceum Bissett, Kubicek & Szackacs, Can. J. Bot. 8: 8, 00. F... more 7. Trichoderma erinaceum Bissett, Kubicek & Szackacs, Can. J. Bot. 8: 8, 00. Figure 9 Section— Trichoderma Description: Colony: 7.0– 8.5 cm growth was observed in 4 days on PDA medium. White mycelium producing yellow pustules turning to dark green color with the age. The pustules uniformly spread throughout the plate leaving a concentric green ring at the center. The reverse of the plate is colorless. Conidiophores: The main axis terminates in a septate elongation with a single phialide at its tip and fertile branches arising near the base. Conidiophore branches arising at angles of 90° or less concerning the main axis, paired or not, rebranching sparingly to produce phialides directly or at the tips of short secondary branches. Phialides: Phialides arising from branches are solitary or in whorls of 2 or 3, straight, cylindrical, flask-shaped and swollen in the middle. Conidia: Ellipsoidal to broadly ellipsoidal, 4.00–5.50 × 3.5–4.5 µm, green. Chlamydospores: Chla...

0. Trichoderma virens (Miller, Giddens & Foster) Arx, Nova Hedwigia Beih. 87: ... more 0. Trichoderma virens (Miller, Giddens & Foster) Arx, Nova Hedwigia Beih. 87: 88, 987. Figure Section— Pachybasium Description: Colony: 6.0–7.0 cm growth was observed in 4 days on PDA medium. Conidiation effuse covering the entire surface of the plate, or forming spreading, light yellowish green flat pustules concentrated near the margin, later turning dark green. Reverse colourless and sometimes drab coloured. Conidiophores: Conidiophores arising in clusters in lateral branches from undifferentiated aerial mycelium, at the base sterile and un-branched, but the upper part fertile toward the apex,each branch terminating in a penicillus of (2–)3–6 closely appressed phialides. The entire branching system irregular and uncrowded. Phialides: Phialides mainly arising in closely appressed whorls of 2–5 on terminal branches, less frequently in pairs or singly, straight, lageniform to ampulliform and sometimes laginiform to subulate, base constricted, swollen in the middle...

Journal of Plant Pathology, 2014

Aloe vera (Aloe barbadensis Miller) is a drought-resistant perennial succulent plant of the famil... more Aloe vera (Aloe barbadensis Miller) is a drought-resistant perennial succulent plant of the family Liliaceae. In September 2013, plants exhibiting severe leaf blight symptoms were observed in the Experimental Farm of the Indian Agricultural Research Institute (IARI), New Delhi. The disease appeared as small circular brown lesions on the leaves which soon developed into dark-brown diffuse spots that turned grey at the centre. The spots frequently coalesced into extended patches, blightening the leaves and, gradually, the entire foliage. Isolation on potato dextrose agar (PDA) (Rao et al., 1991) yielded colonies that were initially white, but later turned grey to greyish black and produced dark-brown pycnidia. Pycniospores were brown, clavate, straight, aseptate, thick-walled, apex-obtuse, tapered to a truncate base and measured 30-45x10- 16 μm. Based on morphology, the fungus was identified as Sphaeropsis sapinea. To confirm the identification, internal transcribed spacer (ITS) regio...

Journal of Environmental Science and Health, Part B, 2020

Abstract A series of ferrocenyl chalcones using acetylferrocene, with ferrocenyl group at the ket... more Abstract A series of ferrocenyl chalcones using acetylferrocene, with ferrocenyl group at the keto carbonyl group, and different aldehydes were synthesized and their bioefficacy evaluation was done against Sclerotium rolfsii, Alternaria solani and Meloidogyne incognita. In continuation of our quest for potent crop protection products, in the present study, a series of 18 substituted ferrocenyl chalcones were synthesized in which ferrocenyl group was attached to the aldehyde moiety, using ferrocenecarboxyaldehyde and different acetophenones by microwave method (MM) and conventional method (CM) [cf: MM 1 to 5 min; CM 12–40 h] and characterized by various techniques viz. IR, LC-HRMS, 1H-NMR and 13C-NMR. In vitro fungicidal activity showed that compound, (2E)-1-(5-Chloro-2-hydroxyphenyl)-3-ferrocenyl-prop-2-en-1-one (34) (ED50 = 21.50 mg L−1) was found to be most active against S. rolfsii and compound, (2E)-1-(4-Bromophenyl)-3-ferrocenyl-prop-2-en-1-one (21) (ED50 = 31.14 mg L−1) showed highest activity against A. solani. As regards nematicidal activity, compound (2E)-1-(3-Bromophenyl)-3-ferrocenyl-prop-2-en-1-one (29) was more potent with LC50 values of 11.95, 8.07 and 4.34 mg L−1 at 24, 48 and 72 h, respectively. QSAR study revealed that MLR for S. rolfsii (r 2 = 0.9834, q 2= 0.8975) and A. solani (r 2 = 0.9807, q 2= 0.8713) and PLS for M. incognita (r 2 = 0.9023, q 2= 0.7818) were the best models.

Indian Phytopathology, 2021

Phytotaxa

Trichoderma speies are known for their diverse applications as potent bio-control organisms. 34 i... more Trichoderma speies are known for their diverse applications as potent bio-control organisms. 34 isolates of Trichoderma were obtained from various geographical locations in India and were subjected to phenotypic evaluation. Species identification if based on only morphology, may lead to sometimes erroneous, uncertain or remains unclear due to overlapping characters. Therefore, molecular characterization was also performed based on ITS region sequence analysis for reliable identification using both morphological and molecular characters. The isolates were identified into 20 different species viz., T. aggressivum, T. asperellum, T. atroviride, T. brevicompactum, T. citrinoviride, T. crassum, T. erinaceum, T. ghanense, T. hamatum, T. harzianum, T. koningiopsis, T. longibrachiatum, T. longipile (Syn. Hypocrea longipilosa), T. minutisporum, T. pubscenes, T.reesei, T. saturnisporum, T. spirale, T. tomentosum and T. virens based on the integrated approach of both morphological and molecula...

FIGURE 8. Trichoderma reesei (A) Growth on PDA, (B) Pustules, (C) Reverse of the plate, (D,E,F) C... more FIGURE 8. Trichoderma reesei (A) Growth on PDA, (B) Pustules, (C) Reverse of the plate, (D,E,F) Conidiophore branching, (G,H) Phialide disposition, (I) Spores, (J) Chlamydospores.

Journal of environmental biology, 2015

Different isolates of Trichoderma were isolated from soil samples which were collected from diffe... more Different isolates of Trichoderma were isolated from soil samples which were collected from different part of India. These isolates were grouped into four Trichoderma species viz., Trichoderma asperellum (Ta), T. harzianum (Th), T. pseudokoningii (Tp) and T. longibrachiatum (Tl) based on their morphological characters. Identification of the above isolates was also confirmed through ITS region analysis. These Trichoderma isolates were tested for in vitro biological control of Alternaria solani, Bipolaris oryzae, Pyricularia oryzae and Sclerotinia scierotiorum which cause serious diseases like early blight (target spot) of tomato and potato, brown leaf spot disease in rice, rice blast disease, and white mold disease in different plants. Under in vitro conditions, all the four species of Trichoderma (10 isolates) proved 100% potential inhibition against rice blast pathogen Pyracularia oryzae. T. harzianum (Th-01) and T. asperellum (Ta-10) were effective with 86.6% and 97.7%, growth inh...

African journal of microbiology research

The study of morphology and bioefficacy of Trichoderma was undertaken to select the effective iso... more The study of morphology and bioefficacy of Trichoderma was undertaken to select the effective isolates against soil-borne pathogens. Fifty one (51) isolates (23 isolates of Trichoderma virens and 28 isolates of Trichoderma harzianum) were morphologically characterised based on the growth characteristics on PDA medium, the size and shape of phialides and conidia.These isolates were screened for bioefficacy against soil borne plant pathogens (Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii) based on their percent inhibition observed during dual culture, volatile and non-volatile methods. Eight T. virens isolates and 12 T. harzianum isolates were proven to be potential isolates against the soil-borne pathogens tested. No correlation was found between bioefficacy and morphology in both species isolates.

Journal of Environmental Biology, 2021

Aim: Morpho-molecular analyses for taxonomic characterization of nine predominant Penicillium spe... more Aim: Morpho-molecular analyses for taxonomic characterization of nine predominant Penicillium species present in the soil of different parts of India. Methodology: Fifteen Penicillium isolates were isolated from the soil samples collected from the experimental field of ICAR-Indian Agricultural Research Institute (IARI), New Delhi. Another twenty-six isolates were procured from Indian Type Culture Collection (ITCC), Division of Plant Pathology, ICAR-IARI, New Delhi which were isolated from the soil of different parts of India. Total 41 isolates were characterized following distinct macroscopic (colony texture, colony colour exudate production; soluble pigmentation; reverse coloration and mycelial growth) and microscopic observations (type of penicillus; shape of phialides; conidial shape, size and pigmentation). Molecular characterization was done using partial β-tubulin gene sequence which is considered an excellent marker in differentiating Penicillium species. Results: The morphol...

Indian journal of experimental biology, 2013

A total of 75 isolates belonging to five different species of Trichoderma viz., T. asperellum, T.... more A total of 75 isolates belonging to five different species of Trichoderma viz., T. asperellum, T. harzianum, T. longibrachiatum, T. pseudokoningii and T. virens were screened for the production of silver nanoparticles. Although all the isolates produced nanoparticles, T. virens VN-11 could produce maximum nanoparticles as evident from the UV-Vis study. The highest Plasmon band was observed at 420 nm at every 24 h that attained maximum intensity at 120 h (0.543). The high resolution transmission electron microscopy (HRTEM) further provided the morphology of the nanoparticles. These nanoparticles were found single or aggregated with round and uniform in shape and 8-60 nm in size. The nitrate reductase activity of VN-11 was found to be 150 nmol/h/mL which confirmed the production of silver nanoparticles through reduction of Ag+ to Ag0.

Annals of Plant Protection Sciences, 2017

Curvularia is a widespread air-borne facultative pathogen of soil and plants, which mostly surviv... more Curvularia is a widespread air-borne facultative pathogen of soil and plants, which mostly survive as a saprophyte in tropical and sub-tropical regions. It is a dematiceous, filamentous fungus. Curvularia spp. are darkly pigmented fungi with spores (curved conidia) efficiently adapted for most aerial dissemination. A set of 52 Curvularia isolates from Delhi-NCR region, ITCC, MTCC and NFCCI were collected and molecularly characterized and confirmed using ITS sequences from NCBI database as C. aeria, C. affinis, C. australiensis, C. borreriae, C. catenulate, C. clavata, C. eragrostidis, C. geniculate, C. inaequalis, C. lunata, C. pallescens, C. prasadii, C. specifera, C. trifolli, C. tuberculata and C. verruculosa. Identity of these isolates was confirmed through NCBI data base using ITS region sequences. The identification percentage was found to be 90–100%

Indian phytopathology, 2014

Different isolates of Ustilaginoidea virens showed variation in the size, colour and shape of the... more Different isolates of Ustilaginoidea virens showed variation in the size, colour and shape of the conidia, length of spines and width of the hyphae. The conidia were globose, irregularly round to elliptical and warty on the surface with diameters ranging from 4.20 to 6.54 µm. The scanning electron microscopy revealed that the conidial wall was echinulated and ornamented with prominent spines. The spines were pointed at the apex or irregularly curved and ranged from 359.9- 994.5 nm long. Based on colony characters isolates of U. virens were grouped into three groups. The maximum colony diameter was observed in isolate UV3 (40 mm) while minimum was in UV7 (25 mm). The width of the hyphae in different isolates varied from 1.26-2.81 µm. The maximum width of the hypha was 2.81 µm in UV4 isolate and minimum in UV3 (1.26 µm). The genetic diversity of the eight isolates of U. virens, by random amplified polymorphic DNA (RAPD) marker using nine primers, revealed a considerable level of genet...