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Papers by Deepti Pillai
Journal of veterinary medical education, 2021
Practical skills are essential in the veterinary nursing curriculum. Given the increasing impleme... more Practical skills are essential in the veterinary nursing curriculum. Given the increasing implementation of video recording in higher education, this study explored the feasibility and benefits of video recording as a classroom tool in professional education. Concerns regarding the inability to monitor individual students' performance during their laboratory course promoted the implementation of video recording-a blended learning method-in a veterinary nursing course. The approach was personalized for this study, particularly for the Gram staining skill. Students submitted video recordings demonstrating the progression of their skills development, and the instructor reviewed the recordings for assessment. The Participant Perception Indicator, a self-assessment, was used to determine students' experience, knowledge, and confidence gained after performing the skill. Video recording helped students to identify areas for self-improvement. It is also a helpful tool for instructor...
Veterinary Research
This work modifies a loop-mediated isothermal amplification (LAMP) assay to detect the bovine res... more This work modifies a loop-mediated isothermal amplification (LAMP) assay to detect the bovine respiratory disease (BRD) bacterial pathogens Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in a colorimetric format on a farm. BRD causes a significant health and economic burden worldwide that partially stems from the challenges involved in determining the pathogens causing the disease. Methods such as polymerase chain reaction (PCR) have the potential to identify the causative pathogens but require lab equipment and extensive sample processing making the process lengthy and expensive. To combat this limitation, LAMP allows accurate pathogen detection in unprocessed samples by the naked eye allowing for potentially faster and more precise diagnostics on the farm. The assay developed here offers 66.7–100% analytical sensitivity, and 100% analytical specificity (using contrived samples) while providing 60–100% concordance with PCR results when tested on five steers in...
Fusobacterium necrophorum, a Gram-negative anaerobe, is the primary etiologic agent of liver absc... more Fusobacterium necrophorum, a Gram-negative anaerobe, is the primary etiologic agent of liver abscesses of beef cattle. The bacterium, a member of the microbial community of the rumen, travels to the liver via portal circulation to cause abscesses. The severity of liver abscesses vary from mild with one or two small abscesses to severe with medium to large multiple abscesses. Leukotoxin, a secreted protein, is the critical virulence factor involved in the infection. Our objective was to compare leukotoxin production between strains of F. necrophorum isolated from mild and severe liver abscesses collected from slaughtered cattle. The quantification of leukotoxin was based on assays to measure cytotoxicity and protein antigen concentration. One-hundred strains, 50 from mild and 50 from severe abscesses, were utilized in the study. Cell-free supernatants were prepared from cultures grown in anaerobic broth at 9 and 24 h incubations. The leukotoxic activity was quantified by measuring cy...
Journal of veterinary medical education, 2021
Practical skills are essential in the veterinary nursing curriculum. Given the increasing impleme... more Practical skills are essential in the veterinary nursing curriculum. Given the increasing implementation of video recording in higher education, this study explored the feasibility and benefits of video recording as a classroom tool in professional education. Concerns regarding the inability to monitor individual students' performance during their laboratory course promoted the implementation of video recording-a blended learning method-in a veterinary nursing course. The approach was personalized for this study, particularly for the Gram staining skill. Students submitted video recordings demonstrating the progression of their skills development, and the instructor reviewed the recordings for assessment. The Participant Perception Indicator, a self-assessment, was used to determine students' experience, knowledge, and confidence gained after performing the skill. Video recording helped students to identify areas for self-improvement. It is also a helpful tool for instructor...
Veterinary Research
This work modifies a loop-mediated isothermal amplification (LAMP) assay to detect the bovine res... more This work modifies a loop-mediated isothermal amplification (LAMP) assay to detect the bovine respiratory disease (BRD) bacterial pathogens Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in a colorimetric format on a farm. BRD causes a significant health and economic burden worldwide that partially stems from the challenges involved in determining the pathogens causing the disease. Methods such as polymerase chain reaction (PCR) have the potential to identify the causative pathogens but require lab equipment and extensive sample processing making the process lengthy and expensive. To combat this limitation, LAMP allows accurate pathogen detection in unprocessed samples by the naked eye allowing for potentially faster and more precise diagnostics on the farm. The assay developed here offers 66.7–100% analytical sensitivity, and 100% analytical specificity (using contrived samples) while providing 60–100% concordance with PCR results when tested on five steers in...
Fusobacterium necrophorum, a Gram-negative anaerobe, is the primary etiologic agent of liver absc... more Fusobacterium necrophorum, a Gram-negative anaerobe, is the primary etiologic agent of liver abscesses of beef cattle. The bacterium, a member of the microbial community of the rumen, travels to the liver via portal circulation to cause abscesses. The severity of liver abscesses vary from mild with one or two small abscesses to severe with medium to large multiple abscesses. Leukotoxin, a secreted protein, is the critical virulence factor involved in the infection. Our objective was to compare leukotoxin production between strains of F. necrophorum isolated from mild and severe liver abscesses collected from slaughtered cattle. The quantification of leukotoxin was based on assays to measure cytotoxicity and protein antigen concentration. One-hundred strains, 50 from mild and 50 from severe abscesses, were utilized in the study. Cell-free supernatants were prepared from cultures grown in anaerobic broth at 9 and 24 h incubations. The leukotoxic activity was quantified by measuring cy...