Clare Dempsey - Academia.edu (original) (raw)
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Papers by Clare Dempsey
FEBS Letters, 1994
FarR (formerly P30) has been identified as a fatty acid and fatty acyl-CoA responsive DNA-binding... more FarR (formerly P30) has been identified as a fatty acid and fatty acyl-CoA responsive DNA-binding protein. It is encoded by the farR gene (g30) in the citric acid cycle gene cluster of E. coli (gltA-sdhCDAB-sucABCD-farR). The amplified FarR protein specifically bound to the farR promoter (P&J and exhibited weak binding to the citrate synthase and lipoamide dehydrogenase promoters. Binding at PfWR was abolished by long-chain fatty acids and their CoA thioesters. In DNaseI footprints, FarR binding at PfO,R p rotected two sites, each characterised by two related IO-bp direct repeats. It is suggested that FarR autoregulates farR expression and may modulate citric acid cycle expression in response to long-chain fatty acids.
FEBS Letters, 1994
FarR (formerly P30) has been identified as a fatty acid and fatty acyl-CoA responsive DNA-binding... more FarR (formerly P30) has been identified as a fatty acid and fatty acyl-CoA responsive DNA-binding protein. It is encoded by the farR gene (g30) in the citric acid cycle gene cluster of E. coli (gltA-sdhCDAB-sucABCD-farR). The amplified FarR protein specifically bound to the farR promoter (P&J and exhibited weak binding to the citrate synthase and lipoamide dehydrogenase promoters. Binding at PfWR was abolished by long-chain fatty acids and their CoA thioesters. In DNaseI footprints, FarR binding at PfO,R p rotected two sites, each characterised by two related IO-bp direct repeats. It is suggested that FarR autoregulates farR expression and may modulate citric acid cycle expression in response to long-chain fatty acids.