Devanand Fulzele - Academia.edu (original) (raw)

Papers by Devanand Fulzele

Indian Journal of Pharmaceutical Education and Research, 2022

Environmental pollution especially with heavy metals poses serious problem on product synthesis o... more Environmental pollution especially with heavy metals poses serious problem on product synthesis of medicinal plants cultivated in the field. Accordingly, the purpose of this study was to assess in vitro effects of cadmium as abiotic elicitor on phytoestrogens production by Psoralea corylifolia hairy roots. Transformed hairy roots were developed by genetic transformation using Agrobacterium rhizogenes. The stable, transformed and fast growth hairy roots demonstrated high growth rate 7.23% dry weight in growth regulators free Murashige and Skoog's medium enriched with 8 µM of cadmium. Hairy roots were tested with low and high concentrations of cadmium. Correlation of in vitro cadmium exposure and phytoestrogens production showed that low percentage of cadmium and exposure period was stimulated product synthesis, while high levels of cadmium in nutrient medium and more exposure period impaired the product synthesis. Elevated concentrations of cadmium and increased exposure time dec...

Sucrose is a major ingredient of culture medium. Present study was performed to investigate the i... more Sucrose is a major ingredient of culture medium. Present study was performed to investigate the influence of sucrose concentration on growth and ajmalicine production. Maximum packed cell volume (72%), fresh weight (65g 100 ml-1), and dry weight (6.2148g 100 ml-1) was recorded in medium containing 4% sucrose. About 9-fold increase in dry weight was recorded in medium with 4% sucrose. All growth parameters increased upto 25th day of incubation after which a gradual decline was observed. Ajmalicine accumulation started from 15th day of incubation and reached to maximum (60 μg g-1 dry weight) in 25th day of incubation in medium with 4% sucrose, which however gradually reduced on further incubation.

Journal of Plant Growth Regulation

Biotechnology Journal International

Aims: The cosmic production of biomass and bioactive compounds at pilot scale with minimum produc... more Aims: The cosmic production of biomass and bioactive compounds at pilot scale with minimum production costs is an important task to achieve feasible production process of corresponding secondary metabolites at a commercial level. Materials and Methods: The cell suspension cultures of Catharanthus roseus in MS medium supplemented with 2, 4-dichlorophenoxyacetic acid (9.05 µM), kinetin (4.52 µM) were scaled up in a pilot plant bioreactor (100 lit). The cost of production was reduced by addition of substitute carbon source in a basal medium which hardly costs 30% in the medium. Preliminary studies were performed in the 7-lit bioreactor. A 100 lit stainless steel bioreactor equipped with helical impeller top mounted was used for scale-up of C. roseus suspension cultures and ajmalicine production.

Journal of Ayurveda and integrative medicine, Jan 24, 2017

Oldenlandia umbellata L. (Indian madder) is an antique Ayurvedic Indian herb and a source of vari... more Oldenlandia umbellata L. (Indian madder) is an antique Ayurvedic Indian herb and a source of various anthraquinone derivatives. The red dye from its roots has been used in diverse applications since ancient times. To establish reliable and effective protocols for in vitro flowering of O. umbellata. For in vitro flowering, organogenic calli were subcultured onto Murashige and Skoog (MS) medium supplemented with various concentrations of Naphthalene acetic acid (NAA) (0.15-1.0 mg/l) and Benzyladenine(BA) (0.5-1.5 mg/l) with and without 0.4% of coconut milk (CM). The highest number of in vitro flowers (22.8%) and best response (92.73%) was achieved on MS medium supplemented with 0.7 mg/l NAA + 1.5 mg/l BA with 0.4% CM. It was found that MS medium devoid of BA promoted best root development (47.3 per calli) as well as response (100%). It was also observed that when embryogenic calli grown in depletion of required nutrition transferred to fresh media induced more flowering. In vivo and i...

Plant Cell, Tissue and Organ Culture (PCTOC)

Limonium ‘Misty Blue’ is an interspecific hybrid of Limonium latifolium and L. bellidifolium and ... more Limonium ‘Misty Blue’ is an interspecific hybrid of Limonium latifolium and L. bellidifolium and has a huge demand in floriculture business as both fresh and dry flowers with stunning purple-blue blooms. The propagation only through vegetative means restrict the popularization of this plant to the flower growers. We therefore optimized an efficient micropropagation protocol for direct organogenesis from root explants, as leaf is not conducible to respond in culture. 61.43% of root explants directly formed shoot buds on their surface after 4-weeks of culture in media containing ½ MS, 43.82 mM sucrose 2.22 µM BA and 1.07 µM NAA. The shoot buds failed to differentiate into healthy shoots unless the previous medium was replaced by full strength MS, and 87.64 mM sucrose along with 0.44 µM BA and 1.07 µM NAA. Encapsulations of juvenile shoots were carried out by 3% sodium alginate and 100 mM CaCl2 which were again successfully stored at 4 °C for 30 days along with 56.79% of plant recovery in MS + 0.44 µM BA + 4.5 µM IBA + 87.64 mM sucrose containing medium. 150 synthetic seed derived full grown plants were successfully acclimatized in green house, where a total of 101 plants survived after secondary hardening. The ISSR analysis revealed genetic homogeneity of synthetic seed derived hardened plants.

Camptothecin, topoisomerase I-DNA inhibitor, has been evaluated in methanolic extract of various ... more Camptothecin, topoisomerase I-DNA inhibitor, has been evaluated in methanolic extract of various parts of Nothapodytes nimmoniana (J. Graham) Mabberly (Family Icacinaceae) collected in the month of February from different regions (Mahabaleshwar and Patan regions of Maharashtra state and Sirsi region of Karnataka state) of Western Ghats, India, using high performance liquid chromatography. Quantification was performed with the regression analysis and the method was validated as per International Conference of Harmonization (ICH) guidelines. In regard to various plant parts, maximum concentration of camptothecin was found in root (2.62%) collected from Mahabaleshwar, Patan (1.21%) and Sirsi (0.88%) regions followed by stem collected from Patan (1.45%), Sirsi (0.70%) and Mahabaleshwar (0.43%) regions. The lowest concentration of camptothecin was found in leaves collected from Sirsi (0.29) region, followed by Patan (0.37) and Mahabaleshwar (0.70%) region. Fruits collected from Mahabaleshwar region contain maximum concentration of camptothecin (0.63%) whereas fruits from Patan region contain minimum concentration of camptothecin (0.36%). There was a 2-fold higher concentration of camptothecin observed in roots from Mahabaleshwar region than roots from Sirsi and Patan region. So it is evident that geographical and climatic conditions have remarkable influence in the content of camptothecin in N. nimmoniana.

High frequency shoot regeneration and evaluation of product synthesis at various stages in Psoral... more High frequency shoot regeneration and evaluation of product synthesis at various stages in Psoralea corylifolia were investigated. In vitro regenerated shoots were induced from germinated seedling on MS medium supplemented with 2, 4, 8, 20 and 40 µM of thidiazuron and N 6-benzylaminopurine. The results revealed that optimum concentrations of thidiazuron (8 µM) into the medium increased shoot regeneration frequency. Root differentiation was achieved from regenerated shoots on growth regulator free MS medium with frequency of 91.2% and mean number of 4.5 roots per shoot. High concentrations of Indole-3-aceticacid (IAA) into the rooting medium resulted in slow growth. Regenerated shoots and roots enhanced isoflavones production compared to field grown plants. A reverse phase high performance liquid chromatography analysis revealed that in vitro regenerated shoots accumulated 0.85% dry wt of daidzein and 0.06% dry wt of genistein. Maximum daidzein (1.23% dry wt) and genistein (0.38% dry wt) were accumulated by roots which obtained from regenerated shoots, which is 6.3-fold more daidzein and 77-fold more genistein respectively than field grown plants. The regeneration protocol developed successfully in this study showed the possibility for rapid propagation of P. corylifolia and enhanced isoflavones production.

Applied Biochemistry and Biotechnology, 2016

Carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavag... more Carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavage oxygenases (CCOs). The apocarotenoids are economically important as it is known to have therapeutic as well as industrial applications. For instance, bixin from Bixa orellana and crocin from Crocus sativus are commercially used as a food colourant and cosmetics since prehistoric time. In our present study, CCD4a gene has been identified and isolated from leaves of B. orellana for the first time and named as BoCCD4a; phylogenetic analysis was carried out using CLUSTAL W. From sequence analysis, BoCCD4a contains two exons and one intron, which was compared with the selected AtCCD4, RdCCD4, GmCCD4 and CmCCD4a gene. Further, the BoCCD4a gene was cloned into pCAMBIA 1301, transformed into Agrobacterium tumefaciens EHA105 strain and subsequently transferred into hypocotyledons and callus of B. orellana by agro-infection. Selection of stable transformation was screened on the basis of PCR detection by using GUS and hptII specific primer, which was followed by histochemical characterization. The percent transient GUS expression in hypocotyledons and callus was 84.4 and 80 %, respectively. The expression of BoCCD4a gene in B. orellana was confirmed through RT-PCR analysis. From our results, the sequence analysis of BoCCD4a gene of B. orellana was closely related to the CsCCD4 gene of C. sativus, which suggests this gene may have a role in various processes such as fragrance, insect attractant and pollination.

Journal of Basic and Clinical Physiology and Pharmacology, 2015

Oxidative stress in erythrocyte was induced by AAPH. The inhibition of hemolysis, membrane lipid ... more Oxidative stress in erythrocyte was induced by AAPH. The inhibition of hemolysis, membrane lipid peroxidation, and protein damage by chloroform extracts ofApproximately 95% of erythrocytes were lysed by AAPH over 3 h of incubation. Significant reduction in hemolysis was observed by the extracts, and the ICResults indicate that

A bioreactor with helix impeller has been designed and developed at Nuclear Agricultureand Biotec... more A bioreactor with helix impeller has been designed and developed at Nuclear Agricultureand Biotechnology Division capable of growing high density plant cell suspension cultures. The design of this bioreactor has been transferred to Kabra Drugs Limited, Indore under a technology transfer agreement. Mixing studies revealed that the helix impeller speeds of 80 rpm to 200 rpm ensured uniform mixing of swpension cultures. Calharanthw resew cell cultures cultivated in 100 lit bioreactor with an initial 1/,5% packed cell volume to give an initial biomass to liquid ratio 42.6 gr' fresh biomass at Kabra Drugs Limited, Indore. This resulted in production of a biomass 32 Kgfresh weight within 16 days of cultivation, whereas natural grown plant produced 2.3 Kg/m'/year. This work addressed the destgn and development of helix impeller for 50 and 100 lit bioreactor for high density Calharanthw rosew cell swpension cultures.

Leaves of Ficus tsiela were extracted with different solvents and evaluated the phytochemical ana... more Leaves of Ficus tsiela were extracted with different solvents and evaluated the phytochemical analysis and antimicrobial activity. The presence of biological constituents namely terpenoids, tannins, steroids, anthraquinones, flavonoids, saponins, cardiac glycosides and phlobatannin were confirmed through preliminary phytochemical analysis. Alkaloid was absent in all the extracts treated with different solvents investigated. The presence of these bioactive constituents was associated with the antimicrobial activity of the plants. Agar well diffusion method revealed all the solvent extracts exhibited antimicrobial activity. Similarly, no antimicrobial activity was observed with the aqueous extracts, although high activity was exhibited with the ethanol, moderate activity with methanol and mild activity with chloroform extracts. The Gram-negative bacteria appeared to be more susceptible to the antimicrobial effect of the extracts than the Gram-positive organisms. The results confirmed ...

Biotechnology and Bioprocess Engineering, 2015

ABSTRACT The intensity of the hydrodynamic stress on cell aggregates is closely related to the gr... more ABSTRACT The intensity of the hydrodynamic stress on cell aggregates is closely related to the growth and production of secondary metabolites. In this study, we have investigated the effects of cell aggregation in suspension cultures of Psoralea corylifolia on growth and phytoestrogens production. HPLC analysis revealed that cell aggregates of 1.2 mm size produced maximum amount of daidzein (2.84% dry wt.) and genistein (0.47% dry wt.) on day 20. The phytoestrogens production level was ~2-fold more than that of heterogeneous cell aggregates (control) in suspension cultures and 11-fold more than field grown plants. Analysis of spent medium revealed no leaching of phytoestrogens. Results indicated that certain degree of cell aggregation in suspension cultures directly supported phytoestrogen production.

Environmental Toxicology, 2015

Natural toxins from plant sources with wide ranges of biological activities reflect the upswing o... more Natural toxins from plant sources with wide ranges of biological activities reflect the upswing of drug design in the pharmaceutical industry. Rubia cordifolia L. is one of the most important red dye yielding plants. Most of the former researches have focused on the bioactive compounds from the roots of R. cordifolia, while no attention was paid towards the fruits. For the first time, here we report the presence of dibutyl phthalate in the fruits of R. cordifolia. Structural characterization was carried out using Ultraviolet-Visible spectrophotometer (UV-Vis), Fourier transform infrared (FTIR), gas chromatography-mass spectrophotometer (GC-MS), Nuclear magnetic resonance (NMR). Acute toxicity of the crude ethanolic extracts of the R. cordifolia fruits was examined in Swiss albino mice. No mortality was observed in all treated mice with 100, 500, 1000 mg/kg body weight of crude extract of R. cordifolia fruit and it indicates that the LD50 value is higher than 1000 mg/kg body weight. This study exhibited a significant change in the body weight. Alanine transaminase (ALT), total protein, triglycerides, glucose, and also the histopathological analysis of liver for all treated mice showed difference from the control group. The dibutyl phthalate was further evaluated for the toxicity study through in silico analysis. Together, the results highlighted that the toxic potential of R. cordifolia fruits extracts and also the toxicity profile of the fruit should be essential for the future studies dealing with the long term effect in animals. © 2015 Wiley Periodicals, Inc. Environ Toxicol, 2015.

Untransformed root cultures of Nothapodytes foetida were established from immature zygotic embryo... more Untransformed root cultures of Nothapodytes foetida were established from immature zygotic embryos on MS basal medium supplemented with different concentration of growth regulators. Alkaloid contents in untransformed root cultures showed that root elongation and growth regulators accompanied product synthesis. Basal medium supplemented with NAA and BA achieved maximum number of elongated roots. Maximum concentration of camptothecin (0.01% DW) and 9-methoxy camptothecin (0.0016% DW) were synthesised by untransformed root cultures incubated on MS medium supplemented with NAA (71.36 µM) and BA (8.87 µM). Culture medium containing NAA (71.36 µM) and Kn (9.29 µM) proliferated callus interspersed with roots which synthesised camptothecin (0.00017% DW) and 9-methoxy-camptothecin (0.000058% DW).

The effect of modified Zarrouk's medium on the growth response of 6 different Spirulina strains w... more The effect of modified Zarrouk's medium on the growth response of 6 different Spirulina strains was evaluated. Specific growth rate, doubling time, mean daily division rate, biomass, and chlorophyll-A contents were analyzed. Growth patterns of these strains were monitored continuously for 40 days. The results revealed significant differences in the growth parameters for different strains. S. platensis (SP-6) and S. platensis (CCMB) showed the maximum specific growth rates (µ = 6.1, µ = 5.8), doubling times (Td = 6.93, Td = 6.87), mean division rates (k = 0.27, k = 0.23) biomasses (5.1, 5.0 g/l) and chlorophyll A contents (78, 65 µg/ml) respectively, when compared with the other strains used in this study. Therefore, S. platensis (SP-6) and S. platensis (CCMB) strains can be suggested for large scale commercial cultivation with modified Zarrouk's medium. This provides the basis of a low cost medium for cultivating Spirulina, which is known to be a promising microalgae with several benefits.

Journal of Natural Medicines, 2010

Transformed hairy roots of Psoralea corylifolia were established by infection with Agrobacterium ... more Transformed hairy roots of Psoralea corylifolia were established by infection with Agrobacterium rhizogenes LBA 9402. The aim of this work was to elucidate the effects of media constituents on production of the phytoestrogenic isoflavones daidzein and genistein. A. rhizogenes strain LBA 9402 harboring Ri plasmid was used to transform stem segments of in vitro seedlings. The resultant hairy roots were confirmed by polymerase chain reaction (PCR) and exhibited Ri T-DNA. Transformed hairy root clones were cultured in Murashige and Skoog&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s (MS) medium altered with different concentrations of NH(4) (+) and NO(3) (-) and their growth and production of isoflavones were assessed. Biomass and productivity increased when MS medium was supplemented with NH(4) (+) and NO(3) (-) at a ratio of 20:10. Increased yield of daidzein was obtained when sucrose level in the culture medium increased, whereas decreased level of sucrose favored genistein production. The hairy roots produced the highest levels of daidzein (2.06% dry wt.) and genistein (0.37% dry wt.) in the presence of low concentrations of PO(4) (3-). Hairy roots secreted trace amounts of daidzein and genistein into the culture medium. The present results demonstrated that the productivity of daidzein was 2.2-fold more than that of untransformed roots.

Planta Medica, 2001

Callus cultures were initiated from stem parts of Nothapodytes foetida on Murashige and Skoog&amp... more Callus cultures were initiated from stem parts of Nothapodytes foetida on Murashige and Skoog's medium supplemented with different growth regulators. Suspension cultures were established and the cell biomass was higher in the presence of NAA in comparison with 2,4-D. Culture medium supplemented with NAA (10.74 microM) and BA (2.22 microM) attained 31.3 g/l DW during 20 days of cultivation in shake flasks. In the presence of NAA, maximum concentrations of camptothecin (0.035 mg/ml) and 9-methoxycamptothecin (0.026 mg/ml) were found in the medium. Alkaloid production was reduced in presence of 2,4-D in the culture medium. Cells contained trace amount of alkaloids. Alkaloids were detected and identified by means of TLC and HPLC.