Didier Alard - Academia.edu (original) (raw)
Papers by Didier Alard
Forest Policy and Economics, 2005
In this paper, circumstances where various human activities and interests clash with the conserva... more In this paper, circumstances where various human activities and interests clash with the conservation of forest biodiversity are examined, with particular focus on the drivers behind the conflicts. After identifying past and current human-related threats potentially leading to conflicts in forests, the paper will focus on conflict management and monitoring, with an emphasis on inclusionary stakeholder networks and a range
Acta Botanica Gallica, 2014
Forest edges are key features in human-dominated landscape. Located between forest and non-forest... more Forest edges are key features in human-dominated landscape. Located between forest and non-forest habitats, edges induce biotic and abiotic changes, which may have profound consequences on vegetation diversity. Recent studies suggest the importance of different edge types in the modulation of edge-related responses. However, edge effect on the spatial dynamic of vegetation, from forest to non-forest habitats, remains unclear. Our aim was to compare the species richness and diversity of vegetation communities between forest and open habitats with their respective edges, in highcontrast versus low-contrast situations. The degree of contrast was defined according to the disturbance regimen of nonforest habitats. We surveyed vascular vegetation along transects in forest and open habitats and in their respective edges, in three regions of France. We showed that edge effects occur on plant diversity, whatever the region, but asymmetrically. Edge effect tends to be greater on the open side than on the forest side of the border. Species richness and diversity were generally higher in open edge than in open habitat, whereas no significant difference was observed between forest edge and forest habitat, whatever the contrast situation encountered. This study shows that the edge effects detected along a forest-edge-exterior habitat gradient may depend in large part on the disturbance regimen in open habitats as well as the vegetation pool size. We highlighted the need to carefully consider the edge types, e.g. their contrast with adjoining non-forest habitat, in further studies to identify the relevant factors and mechanisms behind edge-related response patterns of biodiversity in human-dominated landscapes.
Issues in Environmental Science and Technology, 2007
Nitrogen Deposition, Critical Loads and Biodiversity, 2014
Ecosystem services are defined as the ecological and socio-economic value of goods and services p... more Ecosystem services are defined as the ecological and socio-economic value of goods and services provided by natural and semi-natural ecosystems. Ecosystem services are being impacted by many human induced stresses, one of them being nitrogen (N) deposition and its interactions with other pollutants and climate change. It is concluded that N directly or indirectly affects a wide range of provi-sioning, regulating, supporting and cultural ecosystem services, many of which are interrelated. When considering the effects of N on ecosystem services, it is important to distinguish between different types of ecosystems/species and the protection against N impacts should include other aspects related to N, in addition to biodi-495 51 Nitrogen Deposition Effects on Ecosystem Services and Interactions … greenhouse gas ecosystem services and biodiversity protection; up to some point of increasing N inputs, net greenhouse gas uptake is improved, while biodiversity is already adversely affected.
Global Change Biology, 2014
Nutrient pollution presents a serious threat to biodiversity conservation. In terrestrial ecosyst... more Nutrient pollution presents a serious threat to biodiversity conservation. In terrestrial ecosystems, the deleterious effects of nitrogen pollution are increasingly understood and several mitigating environmental policies have been developed. Compared to nitrogen, the effects of increased phosphorus have received far less attention, although some studies have indicated that phosphorus pollution may be detrimental for biodiversity as well. On the basis of a dataset covering 501 grassland plots throughout Europe, we demonstrate that, independent of the level of atmospheric nitrogen deposition and soil acidity, plant species richness was consistently negatively related to soil phosphorus. We also identified thresholds in soil phosphorus above which biodiversity appears to remain at a constant low level. Our results indicate that nutrient management policies biased toward reducing nitrogen pollution will fail to preserve biodiversity. As soil phosphorus is known to be extremely persistent and we found no evidence for a critical threshold below which no environmental harm is expected, we suggest that agro-environmental schemes should include grasslands that are permanently free from phosphorus fertilization.
Water, Air, & Soil Pollution, 2013
Emissions and deposition of ammonia and nitrogen oxides have strongly increased since the 1950s. ... more Emissions and deposition of ammonia and nitrogen oxides have strongly increased since the 1950s. This has led to significant changes in the nitrogen (N) cycle, vegetation composition and plant diversity in many ecosystems of high conservation value in Europe. As a consequence of different regional pollution levels and of the increased importance of reduced N in the near future, determining the effect of different forms of N is an important task for understanding the consequences of atmospheric N inputs. We have initiated three replicated N addition experiments in species-rich, acidic grasslands spanning a climatic gradient in the Atlantic Water Air Soil Pollut (
Restoration Ecology, 1998
Proceedings of the National Academy of Sciences, 2013
In Europe and, increasingly, the rest of the world, the key policy tool for the control of air po... more In Europe and, increasingly, the rest of the world, the key policy tool for the control of air pollution is the critical load, a level of pollution below which there are no known significant harmful effects on the environment. Critical loads are used to map sensitive regions and habitats, permit individual polluting activities, and frame international negotiations on transboundary air pollution. Despite their fundamental importance in environmental science and policy, there has been no systematic attempt to verify a critical load with field survey data. Here, we use a large dataset of European grasslands along a gradient of nitrogen (N) deposition to show statistically significant declines in the abundance of species from the lowest level of N deposition at which it is possible to identify a change. Approximately 60% of species change points occur at or below the range of the currently established critical load. If this result is found more widely, the underlying principle of no harm in pollution policy may need to be modified to one of informed decisions on how much harm is acceptable. Our results highlight the importance of protecting currently unpolluted areas from new pollution sources, because we cannot rule out ecological impacts from even relatively small increases in reactive N deposition. plant ecology | Threshold Indicator Taxon Analysis | gradient survey S ince the 1980s, the key policy tool for the control of pollution in Europe has been the critical load (1). A critical load is defined as a "quantitative estimate of an exposure to one or more pollutants below which significant harmful effects on specified sensitive elements of the environment do not occur according to present knowledge" (1). Empirical critical load values are currently set for pollutants and habitats based primarily on pollution addition experiments and expert judgment, and they were most recently revised in 2010 (2). Critical loads have a central role in pollution management and are used for mapping pollution impacts, controlling and permitting individual pollution sources, and framing international negotiations on transboundary air pollution. They have recently been applied in the United States (3) and Canada (4), and they are under active consideration and development in many parts of the developing world (5).
Pedobiologia, 2003
The effect of canopy composition on soil macro-invertebrate communities in two deciduous temperat... more The effect of canopy composition on soil macro-invertebrate communities in two deciduous temperate forests: a pure beech (Fagus sylvatica L.) and a mixed beech-hornbeam (Carpinus betulus L.) stand was investigated. The initial hypothesis was that heterogeneity of trophic resources within the mixed stand might increase diversity and heterogeneity of soil macro-invertebrate communities at stand level. The macrofauna was sampled in autumn and spring by hand sorting 121 (25 × 25 × 30 cm) soil monoliths regularly distributed within each stand. Earthworms were identified to species level while the remaining macro-invertebrates were identified to family level. Results were analysed by univariate and multivariate (PCA) statistical tools as well as a geostatistical tool. Few differences were observed when comparing the total macro-invertebrate density between sites and dates. In contrast, significant patterns were found for several taxonomic or trophic groups taken separately (e.g. Lumbricidae, detritivore and predator densities were significantly higher in the pure beech stand (PS). The first and the third PCA axes respectively revealed a site and a season effect while the second axis revealed a spatial segregation within the detritivore group as it distinguished high densities of Lumbricidae from those of Isopoda and Diplopoda. The variance of record scores on this axis measured for each site and date revealed that spatial variability of soil macrofauna communities was greater for PS on both dates than for MS. Semi-variance analysis performed on record coordinates on the second axis of the PCA revealed that only macro-invertebrate communities sampled under the pure stand in autumn were spatially structured (autocorrelation range about 32 m). These results do not support the general agreement that resource diversity and patchiness increases soil fauna biodiversity and heterogeneity.
Journal of Vegetation Science, 2009
Questions: (i) Can sampling of soil wood charcoals at high spatial resolution produce new evidenc... more Questions: (i) Can sampling of soil wood charcoals at high spatial resolution produce new evidence concerning the presence of chalk grassland before or during the Neolithic, Bronze and Iron Ages? (ii) Are there correlations between vegetation history and archeological data during these periods at this particular site?
Journal of Vegetation Science, 2011
Question: Does long-term grazing exclusion affect spatial patterns of canopy height, plant specie... more Question: Does long-term grazing exclusion affect spatial patterns of canopy height, plant species and traits in subalpine grassland communities? Are spatial patterns of species and traits similarly affected by grazing exclusion? Are changes in spatial patterns of species associated with changes in species abundances?
Journal of Vegetation Science, 2011
Question: Which environmental variables affect floristic species composition of acid grasslands i... more Question: Which environmental variables affect floristic species composition of acid grasslands in the Atlantic biogeographic region of Europe along a gradient of atmospheric N deposition? Location: Transect across the Atlantic biogeographic region of Europe including Ireland, Great Britain, Isle of Man, and Sweden.
Journal of Applied Ecology, 2004
Disturbances and resource availability are key factors affecting plant diversity in managed fores... more Disturbances and resource availability are key factors affecting plant diversity in managed forests. As disturbance regimes vary among silvicultural systems and may simultaneously affect different types of resources, effects on biodiversity can be unpredictable. 2. We compared the effects of two silvicultural systems on understorey plant diversity, including species composition, structural attributes and functional organization. One hundred and thirty-five phytosociological relevés were sampled from 27 forest stands managed under either a traditional coppice-with-standards (CWS, n = 12) or a 'close-tonature' selective cutting system (SC, n = 15), over similar edaphic conditions. Important environmental factors affecting vegetation were deduced using Ellenberg indicator values. Structural diversity was described using traditional indices of α and β diversity. Guilds were defined within the local pool of species using a set of 14 traits and their relationship with silviculture was assessed using correspondence analysis. 3. Post-logged CWS stands share some compositional and structural characteristics with selectively cut stands, including high species richness and a dominance of early successional species. However the species pool for all coppicing areas was higher than for selectively cut areas, suggesting that the high disturbance frequency occurring in the latter may progressively eliminate the most sensitive species. 4. Functional diversity strongly differs between the two systems. Although it is conserved through the silvicultural cycle in the coppice-with-standards system, some guilds were lacking in selectively cut stands. The most negatively impacted guilds were tree and shrub saplings, prostrated ruderals, shade-tolerant perennials and vernal geophytes. The latter two comprise 'true forest species' which may also be considered as 'coppicingmaintained species'. To reach the same values of guild richness (i.e. number of guilds) or redundancy (i.e. proportion of the maximal species richness within each guild), larger areas were required in SC compared with CWS systems. 5. In the SC system, the high proportion of light reaching the forest floor induced a spectacular spread of blackberries Rubus fruticosus agg., which decreased species richness. It also caused shifts in guild composition: graminoids and ferns grew strongly to the detriment of true forest species. 6. Synthesis and applications . Our results suggest long-term negative effects of selective cutting on both structural and functional plant diversity, compared with coppice-withstandards. Cutting intervals are shorter than recovery times, so that early successional species-dominated communities are maintained. Vernal geophytes and shade-tolerant perennials seem to be limited by the frequency of disturbance rather than by the severity of disturbance. We conclude that, from a biodiversity point of view, this 'close-to-nature' system does not cope with the objective of sustainable forest management. The rotations currently in use do not match natural disturbances very closely and are applied to a managed system rather than a natural forest. Retaining remnants of old coppice woods and extending rotations to at least 50 years are recommended where biodiversity conservation is a goal of forest management.
Global Change Biology, 2011
Although atmospheric nitrogen (N) deposition and climate changes are both recognized as major com... more Although atmospheric nitrogen (N) deposition and climate changes are both recognized as major components of global change, their interaction at ecosystem level is less well understood. A stratified resampling approach was used to investigate the potential impact of changing levels of atmospheric nitrogen deposition and climate change on species composition of nutrient-poor acid grasslands within the French Atlantic Domain (FAD). The study was based on a comparison, over a period of 25 years, of 162 past and present vegetation records assigned to the species-rich Nardus grasslands and distributed in regional community types (CTs). Similarly, the characterization of N deposition and climate was stratified according to (i) past (1980-1990) and present (1995-2005) periods, and (ii) FAD and CT scales. Despite the relatively short time span between sampling periods, significant N deposition and climate changes were detected as well as vegetation changes. Correspondence analysis showed that the relative importance of N deposition and climate in explaining vegetation changes depended on the spatial scale of investigation (FAD vs. local CTs) and the CT. At the FAD scale, the increase of annual mean temperature and decrease of water availability were clearly related to the changes in floristic composition. At the local scale, the most stable CT experienced no significant climate change and a stable load of N deposition, whereas the CTs characterized by the largest floristic changes were associated with dramatic climate changes and moderate loads in both oxidized and reduced N deposition. Despite the narrow gradient of deposition investigated, N deposition was related to significant grassland community changes, depending on the region, i.e. climate context, and on whether N deposition was in the oxidized or reduced form. Our results suggest that N deposition drives grassland composition at the local scale, in interaction with climate, whereas climate changes remain the predominant driver at the FAD scale.
Forest Policy and Economics, 2005
In this paper, circumstances where various human activities and interests clash with the conserva... more In this paper, circumstances where various human activities and interests clash with the conservation of forest biodiversity are examined, with particular focus on the drivers behind the conflicts. After identifying past and current human-related threats potentially leading to conflicts in forests, the paper will focus on conflict management and monitoring, with an emphasis on inclusionary stakeholder networks and a range
Acta Botanica Gallica, 2014
Forest edges are key features in human-dominated landscape. Located between forest and non-forest... more Forest edges are key features in human-dominated landscape. Located between forest and non-forest habitats, edges induce biotic and abiotic changes, which may have profound consequences on vegetation diversity. Recent studies suggest the importance of different edge types in the modulation of edge-related responses. However, edge effect on the spatial dynamic of vegetation, from forest to non-forest habitats, remains unclear. Our aim was to compare the species richness and diversity of vegetation communities between forest and open habitats with their respective edges, in highcontrast versus low-contrast situations. The degree of contrast was defined according to the disturbance regimen of nonforest habitats. We surveyed vascular vegetation along transects in forest and open habitats and in their respective edges, in three regions of France. We showed that edge effects occur on plant diversity, whatever the region, but asymmetrically. Edge effect tends to be greater on the open side than on the forest side of the border. Species richness and diversity were generally higher in open edge than in open habitat, whereas no significant difference was observed between forest edge and forest habitat, whatever the contrast situation encountered. This study shows that the edge effects detected along a forest-edge-exterior habitat gradient may depend in large part on the disturbance regimen in open habitats as well as the vegetation pool size. We highlighted the need to carefully consider the edge types, e.g. their contrast with adjoining non-forest habitat, in further studies to identify the relevant factors and mechanisms behind edge-related response patterns of biodiversity in human-dominated landscapes.
Issues in Environmental Science and Technology, 2007
Nitrogen Deposition, Critical Loads and Biodiversity, 2014
Ecosystem services are defined as the ecological and socio-economic value of goods and services p... more Ecosystem services are defined as the ecological and socio-economic value of goods and services provided by natural and semi-natural ecosystems. Ecosystem services are being impacted by many human induced stresses, one of them being nitrogen (N) deposition and its interactions with other pollutants and climate change. It is concluded that N directly or indirectly affects a wide range of provi-sioning, regulating, supporting and cultural ecosystem services, many of which are interrelated. When considering the effects of N on ecosystem services, it is important to distinguish between different types of ecosystems/species and the protection against N impacts should include other aspects related to N, in addition to biodi-495 51 Nitrogen Deposition Effects on Ecosystem Services and Interactions … greenhouse gas ecosystem services and biodiversity protection; up to some point of increasing N inputs, net greenhouse gas uptake is improved, while biodiversity is already adversely affected.
Global Change Biology, 2014
Nutrient pollution presents a serious threat to biodiversity conservation. In terrestrial ecosyst... more Nutrient pollution presents a serious threat to biodiversity conservation. In terrestrial ecosystems, the deleterious effects of nitrogen pollution are increasingly understood and several mitigating environmental policies have been developed. Compared to nitrogen, the effects of increased phosphorus have received far less attention, although some studies have indicated that phosphorus pollution may be detrimental for biodiversity as well. On the basis of a dataset covering 501 grassland plots throughout Europe, we demonstrate that, independent of the level of atmospheric nitrogen deposition and soil acidity, plant species richness was consistently negatively related to soil phosphorus. We also identified thresholds in soil phosphorus above which biodiversity appears to remain at a constant low level. Our results indicate that nutrient management policies biased toward reducing nitrogen pollution will fail to preserve biodiversity. As soil phosphorus is known to be extremely persistent and we found no evidence for a critical threshold below which no environmental harm is expected, we suggest that agro-environmental schemes should include grasslands that are permanently free from phosphorus fertilization.
Water, Air, & Soil Pollution, 2013
Emissions and deposition of ammonia and nitrogen oxides have strongly increased since the 1950s. ... more Emissions and deposition of ammonia and nitrogen oxides have strongly increased since the 1950s. This has led to significant changes in the nitrogen (N) cycle, vegetation composition and plant diversity in many ecosystems of high conservation value in Europe. As a consequence of different regional pollution levels and of the increased importance of reduced N in the near future, determining the effect of different forms of N is an important task for understanding the consequences of atmospheric N inputs. We have initiated three replicated N addition experiments in species-rich, acidic grasslands spanning a climatic gradient in the Atlantic Water Air Soil Pollut (
Restoration Ecology, 1998
Proceedings of the National Academy of Sciences, 2013
In Europe and, increasingly, the rest of the world, the key policy tool for the control of air po... more In Europe and, increasingly, the rest of the world, the key policy tool for the control of air pollution is the critical load, a level of pollution below which there are no known significant harmful effects on the environment. Critical loads are used to map sensitive regions and habitats, permit individual polluting activities, and frame international negotiations on transboundary air pollution. Despite their fundamental importance in environmental science and policy, there has been no systematic attempt to verify a critical load with field survey data. Here, we use a large dataset of European grasslands along a gradient of nitrogen (N) deposition to show statistically significant declines in the abundance of species from the lowest level of N deposition at which it is possible to identify a change. Approximately 60% of species change points occur at or below the range of the currently established critical load. If this result is found more widely, the underlying principle of no harm in pollution policy may need to be modified to one of informed decisions on how much harm is acceptable. Our results highlight the importance of protecting currently unpolluted areas from new pollution sources, because we cannot rule out ecological impacts from even relatively small increases in reactive N deposition. plant ecology | Threshold Indicator Taxon Analysis | gradient survey S ince the 1980s, the key policy tool for the control of pollution in Europe has been the critical load (1). A critical load is defined as a "quantitative estimate of an exposure to one or more pollutants below which significant harmful effects on specified sensitive elements of the environment do not occur according to present knowledge" (1). Empirical critical load values are currently set for pollutants and habitats based primarily on pollution addition experiments and expert judgment, and they were most recently revised in 2010 (2). Critical loads have a central role in pollution management and are used for mapping pollution impacts, controlling and permitting individual pollution sources, and framing international negotiations on transboundary air pollution. They have recently been applied in the United States (3) and Canada (4), and they are under active consideration and development in many parts of the developing world (5).
Pedobiologia, 2003
The effect of canopy composition on soil macro-invertebrate communities in two deciduous temperat... more The effect of canopy composition on soil macro-invertebrate communities in two deciduous temperate forests: a pure beech (Fagus sylvatica L.) and a mixed beech-hornbeam (Carpinus betulus L.) stand was investigated. The initial hypothesis was that heterogeneity of trophic resources within the mixed stand might increase diversity and heterogeneity of soil macro-invertebrate communities at stand level. The macrofauna was sampled in autumn and spring by hand sorting 121 (25 × 25 × 30 cm) soil monoliths regularly distributed within each stand. Earthworms were identified to species level while the remaining macro-invertebrates were identified to family level. Results were analysed by univariate and multivariate (PCA) statistical tools as well as a geostatistical tool. Few differences were observed when comparing the total macro-invertebrate density between sites and dates. In contrast, significant patterns were found for several taxonomic or trophic groups taken separately (e.g. Lumbricidae, detritivore and predator densities were significantly higher in the pure beech stand (PS). The first and the third PCA axes respectively revealed a site and a season effect while the second axis revealed a spatial segregation within the detritivore group as it distinguished high densities of Lumbricidae from those of Isopoda and Diplopoda. The variance of record scores on this axis measured for each site and date revealed that spatial variability of soil macrofauna communities was greater for PS on both dates than for MS. Semi-variance analysis performed on record coordinates on the second axis of the PCA revealed that only macro-invertebrate communities sampled under the pure stand in autumn were spatially structured (autocorrelation range about 32 m). These results do not support the general agreement that resource diversity and patchiness increases soil fauna biodiversity and heterogeneity.
Journal of Vegetation Science, 2009
Questions: (i) Can sampling of soil wood charcoals at high spatial resolution produce new evidenc... more Questions: (i) Can sampling of soil wood charcoals at high spatial resolution produce new evidence concerning the presence of chalk grassland before or during the Neolithic, Bronze and Iron Ages? (ii) Are there correlations between vegetation history and archeological data during these periods at this particular site?
Journal of Vegetation Science, 2011
Question: Does long-term grazing exclusion affect spatial patterns of canopy height, plant specie... more Question: Does long-term grazing exclusion affect spatial patterns of canopy height, plant species and traits in subalpine grassland communities? Are spatial patterns of species and traits similarly affected by grazing exclusion? Are changes in spatial patterns of species associated with changes in species abundances?
Journal of Vegetation Science, 2011
Question: Which environmental variables affect floristic species composition of acid grasslands i... more Question: Which environmental variables affect floristic species composition of acid grasslands in the Atlantic biogeographic region of Europe along a gradient of atmospheric N deposition? Location: Transect across the Atlantic biogeographic region of Europe including Ireland, Great Britain, Isle of Man, and Sweden.
Journal of Applied Ecology, 2004
Disturbances and resource availability are key factors affecting plant diversity in managed fores... more Disturbances and resource availability are key factors affecting plant diversity in managed forests. As disturbance regimes vary among silvicultural systems and may simultaneously affect different types of resources, effects on biodiversity can be unpredictable. 2. We compared the effects of two silvicultural systems on understorey plant diversity, including species composition, structural attributes and functional organization. One hundred and thirty-five phytosociological relevés were sampled from 27 forest stands managed under either a traditional coppice-with-standards (CWS, n = 12) or a 'close-tonature' selective cutting system (SC, n = 15), over similar edaphic conditions. Important environmental factors affecting vegetation were deduced using Ellenberg indicator values. Structural diversity was described using traditional indices of α and β diversity. Guilds were defined within the local pool of species using a set of 14 traits and their relationship with silviculture was assessed using correspondence analysis. 3. Post-logged CWS stands share some compositional and structural characteristics with selectively cut stands, including high species richness and a dominance of early successional species. However the species pool for all coppicing areas was higher than for selectively cut areas, suggesting that the high disturbance frequency occurring in the latter may progressively eliminate the most sensitive species. 4. Functional diversity strongly differs between the two systems. Although it is conserved through the silvicultural cycle in the coppice-with-standards system, some guilds were lacking in selectively cut stands. The most negatively impacted guilds were tree and shrub saplings, prostrated ruderals, shade-tolerant perennials and vernal geophytes. The latter two comprise 'true forest species' which may also be considered as 'coppicingmaintained species'. To reach the same values of guild richness (i.e. number of guilds) or redundancy (i.e. proportion of the maximal species richness within each guild), larger areas were required in SC compared with CWS systems. 5. In the SC system, the high proportion of light reaching the forest floor induced a spectacular spread of blackberries Rubus fruticosus agg., which decreased species richness. It also caused shifts in guild composition: graminoids and ferns grew strongly to the detriment of true forest species. 6. Synthesis and applications . Our results suggest long-term negative effects of selective cutting on both structural and functional plant diversity, compared with coppice-withstandards. Cutting intervals are shorter than recovery times, so that early successional species-dominated communities are maintained. Vernal geophytes and shade-tolerant perennials seem to be limited by the frequency of disturbance rather than by the severity of disturbance. We conclude that, from a biodiversity point of view, this 'close-to-nature' system does not cope with the objective of sustainable forest management. The rotations currently in use do not match natural disturbances very closely and are applied to a managed system rather than a natural forest. Retaining remnants of old coppice woods and extending rotations to at least 50 years are recommended where biodiversity conservation is a goal of forest management.
Global Change Biology, 2011
Although atmospheric nitrogen (N) deposition and climate changes are both recognized as major com... more Although atmospheric nitrogen (N) deposition and climate changes are both recognized as major components of global change, their interaction at ecosystem level is less well understood. A stratified resampling approach was used to investigate the potential impact of changing levels of atmospheric nitrogen deposition and climate change on species composition of nutrient-poor acid grasslands within the French Atlantic Domain (FAD). The study was based on a comparison, over a period of 25 years, of 162 past and present vegetation records assigned to the species-rich Nardus grasslands and distributed in regional community types (CTs). Similarly, the characterization of N deposition and climate was stratified according to (i) past (1980-1990) and present (1995-2005) periods, and (ii) FAD and CT scales. Despite the relatively short time span between sampling periods, significant N deposition and climate changes were detected as well as vegetation changes. Correspondence analysis showed that the relative importance of N deposition and climate in explaining vegetation changes depended on the spatial scale of investigation (FAD vs. local CTs) and the CT. At the FAD scale, the increase of annual mean temperature and decrease of water availability were clearly related to the changes in floristic composition. At the local scale, the most stable CT experienced no significant climate change and a stable load of N deposition, whereas the CTs characterized by the largest floristic changes were associated with dramatic climate changes and moderate loads in both oxidized and reduced N deposition. Despite the narrow gradient of deposition investigated, N deposition was related to significant grassland community changes, depending on the region, i.e. climate context, and on whether N deposition was in the oxidized or reduced form. Our results suggest that N deposition drives grassland composition at the local scale, in interaction with climate, whereas climate changes remain the predominant driver at the FAD scale.