Diego Souza - Academia.edu (original) (raw)

Papers by Diego Souza

Revista Brasileira De Terapia Intensiva, 2007

JUSTIFICATIVA E OBJETIVOS: Tem sido mostrado que o sulfato de magnésio apresenta benefícios em ad... more JUSTIFICATIVA E OBJETIVOS: Tem sido mostrado que o sulfato de magnésio apresenta benefícios em adultos e crianças asmáticos, com fraca resposta aos agentes b-agonistas e corticóides sistêmicos no departamento de emergência. O objetivo deste estudo foi realizar uma revisão não sistemática acerca do tratamento da asma aguda com sulfato de magnésio por via venosa e nebulização na emergência. CONTEÚDO: As primeiras investigações que demonstraram benefício no uso do sulfato de magnésio na asma são de 1938. Os efeitos do sulfato de magnésio podem ser mediados através da ação antagonista do cálcio ou através da função de co-fator em sistemas enzimáticos envolvendo o fluxo iônico através da membrana celular. Foi realizada uma revisão de literatura através da base de dados MedLine nos últimos seis anos (2000 a 2006) e incluídos os artigos publicados na língua inglesa, através do cruzamento dos unitermos asma e sulfato de magnésio. CONCLUSÕES: A administração de rotina do sulfato de magnésio por via venosa e nebulização em pacientes com asma aguda grave que se apresentam no serviço de emergência não é recomendado; entretanto, como um tratamento adjuvante obtém-se algum benefício.

Revista Brasileira De Anestesiologia, 2010

Background and objectives: Statistical analysis is necessary for adequate evaluation of the origi... more Background and objectives: Statistical analysis is necessary for adequate evaluation of the original article by the reader allowing him/her to better visualize and comprehend the results. The objective of the present study was to determine the frequency of the adequate use of statistical tests in original articles published in the Revista Brasileira The use of statistical tests was deemed appropriate when the selection of the tests was adequate for continuous and categorical variables and for parametric and non-parametric tests, the correction factor was described when the use of multiple comparisons was reported, and the specific use of a statistical test for analysis of one variable was mentioned.

Planta, 2011

Proteins from latex of Calotropis procera (CpLP), Plumeria rubra (PrLP), Carica candamarcensis (P... more Proteins from latex of Calotropis procera (CpLP), Plumeria rubra (PrLP), Carica candamarcensis (P1G10) and Euphorbia tirucalli (EtLP) were tested for antifungal activity against phytopathogens. CpLP and P1G10 inhibited each fungi analyzed. PrLP and EtLP did not exert inhibition. CpLP and P1G10 exhibited preferential inhibitory activity towards R. solani (IC50 = 20.7 and 25.3 µg/ml, respectively). The inhibitory activity was lost after heat treatment or proteolysis, providing evidence for the involvement of proteins in the inhibitory effect. Treatment of CpLP or P1G10 with Dithiothreitol improved both, the endogenous proteolytic activity and the antifungal properties. Conversely, pre-treatment of CpLP or P1G10 with iodoacetamide drastically reduced endogenous proteolytic activities and partially abrogated antifungal activity. Similar results were observed when spores were challenged to germinate in the presence of laticifer proteins. The purified cysteine proteinase CMS2MS2 from Carica candamarcensis latex or papain (E.C. 3.4.22.2), a cysteine proteinase from latex of Carica papaya L., but not trypsin (EC 3.4.21.4) or chymotrypsin (EC 3.4.21.1), two serine proteases, replicated the results obtained with CpLP or P1G10, thus restricting the antifungal property to latex plant cysteine proteinases. CpLP, CMS2MS2 and papain induced production of reactive oxygen species in spores of F. solani, suggesting that inhibition could be linked to oxidative stress. Proteome analysis of CpLP by 2-D electrophoresis and MALDI-TOF-TOF confirmed the existence of various pathogenic-related proteins such as chitinases, peroxidases and osmotins. The results support that laticifer proteins are part of plant defense repertoire against phytopathogenic fungi.

Memorias Do Instituto Oswaldo Cruz, 2009

It has been shown previously that the laticifer fluid of Calotropis procera (Ait.) R.Br. is highl... more It has been shown previously that the laticifer fluid of Calotropis procera (Ait.) R.Br. is highly toxic to the egg hatching and larval development of Aedes aegypti L. In the present study, the larvicidal potential of other laticifer fluids obtained from Cryptostegia grandiflora R.Br., Plumeria rubra L. and Euphorbia tirucalli L. was evaluated. We attempted to correlate larvicidal activity with the presence of endogenous proteolytic activity in the protein fraction of the fluids. After collection, the fluids were processed by centrifugation and dialysis to obtain the soluble laticifer protein (LP) fractions and eliminate water insoluble and low molecular mass molecules. LP did not visibly affect egg hatching at the doses assayed. LP from Cr. grandiflora exhibited the highest larval toxicity, while P. rubra was almost inactive. E. tirucalli was slightly active, but its activity could not be correlated to proteins since no protein was detected in the fluid. The larvicidal effects of LP from C. procera and Cr. grandiflora showed a significant relationship with the proteolytic activity of cysteine proteinases, which are present in both materials. A purified cysteine proteinase (papain) from the latex of Carica papaya (obtained from Sigma) was similarly effective, whereas trypsin and chymotrypsin (both serine proteinases) were ineffective. The results provide evidence for the involvement of cysteine proteinase activity in the larvicidal action of some laticifer fluids. C. procera is an invasive species found in areas infested with Ae. aegypti and thus could prove useful for combating mosquito proliferation. This is the first report to present evidence for the use of proteolytic enzymes as chemical agents to destroy Ae. aegypti larvae.

Arthropod-plant Interactions, 2010

The defensive role of the latex of Calotropis procera has recently been reported. In this study, ... more The defensive role of the latex of Calotropis procera has recently been reported. In this study, latex proteins involved in detrimental effects on insects were evaluated on another important crop pest. The latex was fractionated to obtain its major protein fraction, which was then used to evaluate its insecticidal properties against Callosobruchus maculatus (Coleoptera: Bruchidae) in artificial bioassays. Laticifer proteins (LP) were investigated to characterize their action in such an activity. LP was highly insecticidal at doses as low as 0.1% (W/W). This effect was slightly augmented in F1 generation reared in artificial seeds containing LP at similar proportions of F0, but was fully reversed when F1 developed in LP-free seeds. The insecticidal proteins were not retained in a chitin column, and did not lose their insecticidal activity, even after heat treatment or pronase digestion. However, these samples inhibited papain (EC 3.4.22.2) activity and gut proteases of C. maculatus larvae, and a reverse zymogram showed the presence of protein bands resistant to papain digestion. These activities were not observed in unheated LP as they were probably masked by abundant endogenous cysteine protease (EC 3.4.22.16) activity present in unheated LP. LP was resistant to proteolysis when assayed with C. maculatus gut extract. However, gut proteins of C. maculatus were digested when incubated with LP. These observations and the deleterious effects of LP upon C. maculatus, reinforce the hypothesis that laticifer fluids are involved in plant defense against insects and indicate C. procera latex to be a source of promising insecticidal proteins. The inhibitor of proteolysis present in the latex seems to be resistant to heat and proteolysis and is certainly involved in the detrimental effects observed.

Plant Science, 2010

This study describes the digestive protease activity extracted from the gut of fifth-instar Monar... more This study describes the digestive protease activity extracted from the gut of fifth-instar Monarch butterfly larvae, and its proteolytic activity on latex proteins of their host plant, Calotropis procera (the milkweed) and related non-host species from the milkweed family. Gut extracts digested azocasein, BANA and BApNA. Cysteine protease inhibitors such as E-64 and iodoacetamide inhibited proteolytic activity on azocasein; however, the serine protease inhibitors PMSF and leupeptin were more effective. Gut extracts promptly digested LP and were not affected by endogenous latex proteases. Gut extracts, however, did not digest LP from Cryptostegia grandiflora and only slightly digested LP from Plumeria rubra, two plant species that are not consumed by Monarch larvae. The protein profiles of latex proteins extracted from healthy and attacked plants were different. A protein identified as glycoside hydrolase was detected in increased concentrations in latex from damaged plants. Larvae fed on artificial diets containing 1% or 5% latex proteins were not adversely affected and gained weight faster than control larvae. These results provide new information on the resistance of Monarch larvae fed on C. procera and suggest that the ability of Monarch proteolytic enzymes to promptly digest LP can explain (at least in part) how these insects overcome the defensive proteins found in C. procera latex.

Page 1. DIMENSIONAMENTO DE CARGA-HUMANA BASEADO NA EFICIÊNCIA DE PROFISSIONAIS TERCEIRIZADOS VERS... more Page 1. DIMENSIONAMENTO DE CARGA-HUMANA BASEADO NA EFICIÊNCIA DE PROFISSIONAIS TERCEIRIZADOS VERSUS PROFISSIONAIS VERTICALIZADOS WORKFORCE BALANCE BASED ON THE EFFICIENCY ...

Este artigo descreve as bases para a implementação de um jogo com finalidade pedagógica. Neste, o... more Este artigo descreve as bases para a implementação de um jogo com finalidade pedagógica. Neste, o jogador controla um personagem, o qual tem que interagir com outros e com o ambiente, a fim de resolver problemas propostos pelo jogo. Cada personagem do jogo será moldado como um agente inteligente com tarefas bem definidas, de tal forma que o jogo se constitui em uma sociedade de agentes. Após as devidas considerações sobre RPG e agentes inteligentes, será feita uma discussão acerca da modelagem do jogo empregando agentes.