Dina Abdelrahman - Academia.edu (original) (raw)

Papers by Dina Abdelrahman

Research paper thumbnail of Untitled Item

File contains data of participants like age, results of each detection technique used in the lab.

Research paper thumbnail of Transmission of yellow fever vaccine virus from breast feeding mothers to their infants: reporting of yellow fever virus (YFV) RNA detection in milk specimens

File contains data of participants like age, results of each detection technique used in the lab.

Research paper thumbnail of Comprehensive Analysis of HEXB Protein Reveal Forty Two Novel nsSNPs That May Lead to Sandhoff disease (SD) Using Bioinformatics

ABSTRACTBackgroundSingle Nucleotide Polymorphisms (SNPs) in the HEXB gene are associated with a n... more ABSTRACTBackgroundSingle Nucleotide Polymorphisms (SNPs) in the HEXB gene are associated with a neurodegenerative disorder called Sandhoff disease (SD) (GM2 gangliosidosis-O variant). This study aimed to predict the possible pathogenic SNPs of this gene and their impact on the protein using different bioinformatics tools.MethodsSNPs retrieved from the NCBI database were analyzed using several bioinformatics tools. The different algorithms collectively predicted the effect of single nucleotide substitution on both structure and function of beta subunit beta subunit of both hexosaminidase A and hexosaminidase B proteins.ResultsForty nine mutations were found to be extremely damaging to the structure and function of the HEXB gene protein.ConclusionAccording to this study, forty two novel nsSNP in HEXB are predicted to have possible role in Sandhoff disease using different bioinformatics tools, beside two SNPs found to have effect on miRNAs binding site affecting expression of HEXB gene...

Research paper thumbnail of Detection of Toxoplasma Gondii Antibodies and DNA Among Renal Transplant Recipient and blood donor in Khartoum State, Sudan

International Journal of Scientific Research in Science and Technology, 2021

Background and Aim: Toxoplasma gondii infection arises in transplant recipient groups, but at var... more Background and Aim: Toxoplasma gondii infection arises in transplant recipient groups, but at varying frequencies. Reactivation of latent T. gondii infection in transplant patients is uncommon, but does occur. The incidence of reactivation is greater in patient groups receiving more aggressive immunosuppressive therapy. Early diagnosis and treatment should be considered in T. gondii-antibody-positive patients subjected to solid organ transplantation. The aim of this study was to estimate the seroprevalence of Toxoplasma gondii infection in renal transplant recipients in Khartoum, Sudan, using serological and molecular methods. Methods: This was a descriptive cross sectional, hospital based study, blood sample were collected from 108 participants; out of them 58 renal transplant recipient individuals and 50 healthy Blood donor attending Sudanese Kidney Association Hospital and Sudan Heart Center Blood Bank. Demographic data were collected by structured questionnaire. All samples were...

Research paper thumbnail of Transmission of Yellow fever vaccine virus from breast feeding mothers to their infants: Reporting of YFV RNA Detection in Milk Specimens

Data presented as Excel sheet containing mother's name, age,address, vaccination date, number... more Data presented as Excel sheet containing mother's name, age,address, vaccination date, number of births, their babies age and Lab results.<br>

Research paper thumbnail of SPSS

clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtaine... more clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtained from Soba Teaching Hospital, Police's Hospital, National Laboratory for Public Health, Ear Nose Throat Hospital and Military Hospital in Khartoum State–Sudan. The samples were collected from patients suffering from urinary tract infections, respiratory tract infections, blood infections, wound and ear infections. Data was collected from hospitals records. This data containing file contain the data of all samples included in this study from identification, sensitivity testing until different beta-lactamases genes detection.

Research paper thumbnail of SPSS

clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtaine... more clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtained from Soba Teaching Hospital, Police's Hospital, National Laboratory for Public Health, Ear Nose Throat Hospital and Military Hospital in Khartoum State–Sudan. The samples were collected from patients suffering from urinary tract infections, respiratory tract infections, blood infections, wound and ear infections. Data was collected from hospitals records. This data containing file contain the data of all samples included in this study from identification, sensitivity testing until different beta-lactamases genes detection.

Research paper thumbnail of Transmission of yellow fever vaccine virus from breast feeding mothers to their infants: reporting of yellow fever virus (YFV) RNA detection in milk specimens

F1000Research, 2022

Background: Because of yellow fever’s serious impact on health, vaccination is the principal stra... more Background: Because of yellow fever’s serious impact on health, vaccination is the principal strategy to control the disease. Administration of the yellow fever vaccine to breastfeeding women should be before they complete 9 months post-delivery, in order to prevent transmission of the yellow fever vaccine virus to their infants through breast feeding. This study aimed to confirm whether the excretion of yellow fever vaccine virus is in milk of vaccinated breastfeeding mothers and to confirm the probable transmission to their infants through breast milk. Methods: Samples were taken as follows: one serum specimen was taken 3-14 days after the date of the vaccination, and breast milk specimens were taken at four different time points between 3-4 days apart. Specimens were obtained from eight nursing mothers, who received the YVF vaccine (17DD). Mothers were asymptomatic before and after the vaccine administration but their infants developed symptoms after administration. Maternal seru...

Research paper thumbnail of Insilico Analysis Reveal Three novel nsSNPs May effect onGM2Aprotein Leading to AB variant of GM2 gangliosidosis

ABSTRACTBackgroundAB variant of GM2 gangliosidosis caused as a result of mutations in GM2 activat... more ABSTRACTBackgroundAB variant of GM2 gangliosidosis caused as a result of mutations in GM2 activator gene (GM2A) which is regarded as neurodegenerative disorder. This study aimed to predict the possible damaging SNPs of this gene and their impact on the protein using different bioinformatics tools.MethodsSNPs retrieved from the NCBI database were analyzed using several bioinformatics tools. The different tools collectively predicted the effect of single nucleotide substitution on both structure and function of GM2 activator.ResultsThree novel mutations were found to be highly damaging to the structure and function of theGM2Agene.ConclusionFour SNPs were found to be highly damaging SNPs that affect function, structure and stability ofGM2Aprotein, which they are: C99Y, C112F, C138S and C138R, three of them are novel SNPs (C99Y, C112F and C138S). Also 46 SNPs were predicted to affect miRNAs binding sites on 3’UTR leading to abnormal expression of the resulting protein. These SNPs should...

Research paper thumbnail of Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with ... more Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases genes frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross sectional study conducted in Khartoum State. Eighty isolates were confirmed as Ps. aeruginosa through conventional identification methods and species specific primers. The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase Open Peer Review

Research paper thumbnail of Molecular Detection of Interleukin 28B Gene rs8099917 Polymorphism in chronic HCV Patients from Khartoum State, Sudan

Journal of Antivirals & Antiretrovirals, 2019

Background: Hepatitis C virus (HCV) infection represents a major health burden According to the w... more Background: Hepatitis C virus (HCV) infection represents a major health burden According to the world health organization (WHO) there are more than 170 million people infected worldwide and 3-4 million new infections are estimated per year. Recent studies have demonstrated the role of interleukin B 28(IL B 28) polymorphism in predicting the treatment induced and spontaneous clearance from hepatitis C virus (HCV) infection and genome-wide association studies have shown that single nucleotide polymorphism (SNPs) near interleukin 28B gene are good predictors of response to treatment. The present study aimed to isolate and identify IL 28 B gene rs8099917 polymorphism from HCV infected individuals DNA. Methods: This cross-sectional study was performed on 50 blood samples from patients with chronic HCV infection as detected by ELISA kit (RIBA-11 and c-200/c-22 ELISA Company and country). DNA was extracted from the samples and the frequency of the polymorphism was analyzed by using PCR-RFL...

Research paper thumbnail of The color change of indirect zirconia containing restorative materials – A comparative in vitro study

Egyptian dental journal, 2021

Aim: to evaluate the influence of immersion time in coffee staining solution on the color coordin... more Aim: to evaluate the influence of immersion time in coffee staining solution on the color coordinates of two Zirconia-containing indirect aesthetic materials. Material and methods: 20 Discs were used in the current study; samples have been divided into two groups in accordance with the material used (monolithic Zirconia restoration group [Zirconia] and indirect Zirconia silicate filled composite restoration group [Ceramage]. The samples were prepared and then immersed in coffee [staining solution]. Spectrophotome‌tric analysis was made immediately after manufacturing of discs at (T0), at 7days (T1), 14 days (T2) and at 21days. Results: At T0-T1, T1-T2, T0-T2 and T0-T3, the mean ΔE of Zirconia showed statistically significantly lower value than Ceramage. At T2-T3; no statistically significant difference between mean ΔE of the two ceramic types was found (P-value = 0.155, Effect size = 0.235). Regardless of time; Zirconia showed a statistically significant lower mean ΔE than Ceramage ...

Research paper thumbnail of β-lactamases (bla TEM, bla SHV, bla CTXM-1, bla VEB, bla OXA-1) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

F1000Research, 2021

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with ... more Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases genes frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross sectional study conducted in Khartoum State. Eighty isolates were confirmed as Ps. aeruginosa through conventional identification methods and species specific primers. The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes (blaTEM, blaSHV, blaCTXM-1, blaVEB, blaOXA-1, blaAmpC and blaDHA). Results: Of the 80 confirmed Ps. aeruginosa isolates, 8 (10%) were resistant t...

Research paper thumbnail of Extended spectrum β-lactamases and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

F1000Research, 2020

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with ... more Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases gene (extended spectrum β-lactamases (ESBLs) genes and class C β-lactamases genes) frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross-sectional study conducted in Khartoum State. A total of 80 isolates were confirmed as Ps. aeruginosa through conventional identification methods and species-specific primers (the remaining 40 isolates were other bacterial species). The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes (blaT...

Research paper thumbnail of Thirty two novel nsSNPs May effect on HEXA protein Leading to Tay-Sachs disease (TSD) Using a Computational Approach

Background: Genetic polymorphisms in the HEXA gene are associated with a neurodegenerative disord... more Background: Genetic polymorphisms in the HEXA gene are associated with a neurodegenerative disorder called Tay-Sachs disease (TSD) (GM2 gangliosidosis type 1). This study aimed to predict the possible pathogenic SNPs of this gene and their impact on the protein using different bioinformatics tools. Methods: SNPs retrieved from the NCBI database were analyzed using several bioinformatics tools. The different algorithms collectively predicted the effect of single nucleotide substitution on both structure and function of the hexosaminidase A protein. Results: Fifty nine mutations were found to be highly damaging to the structure and function of the HEXA gene protein. Conclusion: According to this study, thirty two novel nsSNP in HEXA are predicted to have possible role in Tay-Saches Disease using different bioinformatics tools. Our findings could help in genetic study and diagnosis of Tay-Saches Disease. Keywords: Tay-Sachs disease (TSD), GM2 gangliosidosis, hexosaminidase A, HEXA, a n...

Research paper thumbnail of The use of puppets with elementary school children in reducing stigmatizing attitudes towards mental illness

Journal of Mental Health, 2007

Method: Children received a pre and posttest of their attitudes as measured by the re-factored. O... more Method: Children received a pre and posttest of their attitudes as measured by the re-factored. Opinions About Mental Illness Scale. The experimental group watched a series of three plays in which hand puppets portrayed individuals with schizophrenia, depression/ ...

Research paper thumbnail of Untitled Item

File contains data of participants like age, results of each detection technique used in the lab.

Research paper thumbnail of Transmission of yellow fever vaccine virus from breast feeding mothers to their infants: reporting of yellow fever virus (YFV) RNA detection in milk specimens

File contains data of participants like age, results of each detection technique used in the lab.

Research paper thumbnail of Comprehensive Analysis of HEXB Protein Reveal Forty Two Novel nsSNPs That May Lead to Sandhoff disease (SD) Using Bioinformatics

ABSTRACTBackgroundSingle Nucleotide Polymorphisms (SNPs) in the HEXB gene are associated with a n... more ABSTRACTBackgroundSingle Nucleotide Polymorphisms (SNPs) in the HEXB gene are associated with a neurodegenerative disorder called Sandhoff disease (SD) (GM2 gangliosidosis-O variant). This study aimed to predict the possible pathogenic SNPs of this gene and their impact on the protein using different bioinformatics tools.MethodsSNPs retrieved from the NCBI database were analyzed using several bioinformatics tools. The different algorithms collectively predicted the effect of single nucleotide substitution on both structure and function of beta subunit beta subunit of both hexosaminidase A and hexosaminidase B proteins.ResultsForty nine mutations were found to be extremely damaging to the structure and function of the HEXB gene protein.ConclusionAccording to this study, forty two novel nsSNP in HEXB are predicted to have possible role in Sandhoff disease using different bioinformatics tools, beside two SNPs found to have effect on miRNAs binding site affecting expression of HEXB gene...

Research paper thumbnail of Detection of Toxoplasma Gondii Antibodies and DNA Among Renal Transplant Recipient and blood donor in Khartoum State, Sudan

International Journal of Scientific Research in Science and Technology, 2021

Background and Aim: Toxoplasma gondii infection arises in transplant recipient groups, but at var... more Background and Aim: Toxoplasma gondii infection arises in transplant recipient groups, but at varying frequencies. Reactivation of latent T. gondii infection in transplant patients is uncommon, but does occur. The incidence of reactivation is greater in patient groups receiving more aggressive immunosuppressive therapy. Early diagnosis and treatment should be considered in T. gondii-antibody-positive patients subjected to solid organ transplantation. The aim of this study was to estimate the seroprevalence of Toxoplasma gondii infection in renal transplant recipients in Khartoum, Sudan, using serological and molecular methods. Methods: This was a descriptive cross sectional, hospital based study, blood sample were collected from 108 participants; out of them 58 renal transplant recipient individuals and 50 healthy Blood donor attending Sudanese Kidney Association Hospital and Sudan Heart Center Blood Bank. Demographic data were collected by structured questionnaire. All samples were...

Research paper thumbnail of Transmission of Yellow fever vaccine virus from breast feeding mothers to their infants: Reporting of YFV RNA Detection in Milk Specimens

Data presented as Excel sheet containing mother's name, age,address, vaccination date, number... more Data presented as Excel sheet containing mother's name, age,address, vaccination date, number of births, their babies age and Lab results.<br>

Research paper thumbnail of SPSS

clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtaine... more clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtained from Soba Teaching Hospital, Police's Hospital, National Laboratory for Public Health, Ear Nose Throat Hospital and Military Hospital in Khartoum State–Sudan. The samples were collected from patients suffering from urinary tract infections, respiratory tract infections, blood infections, wound and ear infections. Data was collected from hospitals records. This data containing file contain the data of all samples included in this study from identification, sensitivity testing until different beta-lactamases genes detection.

Research paper thumbnail of SPSS

clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtaine... more clinical isolates initially identified as <i>Pseudomonas aeruginosa</i>, were obtained from Soba Teaching Hospital, Police's Hospital, National Laboratory for Public Health, Ear Nose Throat Hospital and Military Hospital in Khartoum State–Sudan. The samples were collected from patients suffering from urinary tract infections, respiratory tract infections, blood infections, wound and ear infections. Data was collected from hospitals records. This data containing file contain the data of all samples included in this study from identification, sensitivity testing until different beta-lactamases genes detection.

Research paper thumbnail of Transmission of yellow fever vaccine virus from breast feeding mothers to their infants: reporting of yellow fever virus (YFV) RNA detection in milk specimens

F1000Research, 2022

Background: Because of yellow fever’s serious impact on health, vaccination is the principal stra... more Background: Because of yellow fever’s serious impact on health, vaccination is the principal strategy to control the disease. Administration of the yellow fever vaccine to breastfeeding women should be before they complete 9 months post-delivery, in order to prevent transmission of the yellow fever vaccine virus to their infants through breast feeding. This study aimed to confirm whether the excretion of yellow fever vaccine virus is in milk of vaccinated breastfeeding mothers and to confirm the probable transmission to their infants through breast milk. Methods: Samples were taken as follows: one serum specimen was taken 3-14 days after the date of the vaccination, and breast milk specimens were taken at four different time points between 3-4 days apart. Specimens were obtained from eight nursing mothers, who received the YVF vaccine (17DD). Mothers were asymptomatic before and after the vaccine administration but their infants developed symptoms after administration. Maternal seru...

Research paper thumbnail of Insilico Analysis Reveal Three novel nsSNPs May effect onGM2Aprotein Leading to AB variant of GM2 gangliosidosis

ABSTRACTBackgroundAB variant of GM2 gangliosidosis caused as a result of mutations in GM2 activat... more ABSTRACTBackgroundAB variant of GM2 gangliosidosis caused as a result of mutations in GM2 activator gene (GM2A) which is regarded as neurodegenerative disorder. This study aimed to predict the possible damaging SNPs of this gene and their impact on the protein using different bioinformatics tools.MethodsSNPs retrieved from the NCBI database were analyzed using several bioinformatics tools. The different tools collectively predicted the effect of single nucleotide substitution on both structure and function of GM2 activator.ResultsThree novel mutations were found to be highly damaging to the structure and function of theGM2Agene.ConclusionFour SNPs were found to be highly damaging SNPs that affect function, structure and stability ofGM2Aprotein, which they are: C99Y, C112F, C138S and C138R, three of them are novel SNPs (C99Y, C112F and C138S). Also 46 SNPs were predicted to affect miRNAs binding sites on 3’UTR leading to abnormal expression of the resulting protein. These SNPs should...

Research paper thumbnail of Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with ... more Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases genes frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross sectional study conducted in Khartoum State. Eighty isolates were confirmed as Ps. aeruginosa through conventional identification methods and species specific primers. The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase Open Peer Review

Research paper thumbnail of Molecular Detection of Interleukin 28B Gene rs8099917 Polymorphism in chronic HCV Patients from Khartoum State, Sudan

Journal of Antivirals & Antiretrovirals, 2019

Background: Hepatitis C virus (HCV) infection represents a major health burden According to the w... more Background: Hepatitis C virus (HCV) infection represents a major health burden According to the world health organization (WHO) there are more than 170 million people infected worldwide and 3-4 million new infections are estimated per year. Recent studies have demonstrated the role of interleukin B 28(IL B 28) polymorphism in predicting the treatment induced and spontaneous clearance from hepatitis C virus (HCV) infection and genome-wide association studies have shown that single nucleotide polymorphism (SNPs) near interleukin 28B gene are good predictors of response to treatment. The present study aimed to isolate and identify IL 28 B gene rs8099917 polymorphism from HCV infected individuals DNA. Methods: This cross-sectional study was performed on 50 blood samples from patients with chronic HCV infection as detected by ELISA kit (RIBA-11 and c-200/c-22 ELISA Company and country). DNA was extracted from the samples and the frequency of the polymorphism was analyzed by using PCR-RFL...

Research paper thumbnail of The color change of indirect zirconia containing restorative materials – A comparative in vitro study

Egyptian dental journal, 2021

Aim: to evaluate the influence of immersion time in coffee staining solution on the color coordin... more Aim: to evaluate the influence of immersion time in coffee staining solution on the color coordinates of two Zirconia-containing indirect aesthetic materials. Material and methods: 20 Discs were used in the current study; samples have been divided into two groups in accordance with the material used (monolithic Zirconia restoration group [Zirconia] and indirect Zirconia silicate filled composite restoration group [Ceramage]. The samples were prepared and then immersed in coffee [staining solution]. Spectrophotome‌tric analysis was made immediately after manufacturing of discs at (T0), at 7days (T1), 14 days (T2) and at 21days. Results: At T0-T1, T1-T2, T0-T2 and T0-T3, the mean ΔE of Zirconia showed statistically significantly lower value than Ceramage. At T2-T3; no statistically significant difference between mean ΔE of the two ceramic types was found (P-value = 0.155, Effect size = 0.235). Regardless of time; Zirconia showed a statistically significant lower mean ΔE than Ceramage ...

Research paper thumbnail of β-lactamases (bla TEM, bla SHV, bla CTXM-1, bla VEB, bla OXA-1) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

F1000Research, 2021

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with ... more Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases genes frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross sectional study conducted in Khartoum State. Eighty isolates were confirmed as Ps. aeruginosa through conventional identification methods and species specific primers. The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes (blaTEM, blaSHV, blaCTXM-1, blaVEB, blaOXA-1, blaAmpC and blaDHA). Results: Of the 80 confirmed Ps. aeruginosa isolates, 8 (10%) were resistant t...

Research paper thumbnail of Extended spectrum β-lactamases and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

F1000Research, 2020

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with ... more Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases gene (extended spectrum β-lactamases (ESBLs) genes and class C β-lactamases genes) frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross-sectional study conducted in Khartoum State. A total of 80 isolates were confirmed as Ps. aeruginosa through conventional identification methods and species-specific primers (the remaining 40 isolates were other bacterial species). The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes (blaT...

Research paper thumbnail of Thirty two novel nsSNPs May effect on HEXA protein Leading to Tay-Sachs disease (TSD) Using a Computational Approach

Background: Genetic polymorphisms in the HEXA gene are associated with a neurodegenerative disord... more Background: Genetic polymorphisms in the HEXA gene are associated with a neurodegenerative disorder called Tay-Sachs disease (TSD) (GM2 gangliosidosis type 1). This study aimed to predict the possible pathogenic SNPs of this gene and their impact on the protein using different bioinformatics tools. Methods: SNPs retrieved from the NCBI database were analyzed using several bioinformatics tools. The different algorithms collectively predicted the effect of single nucleotide substitution on both structure and function of the hexosaminidase A protein. Results: Fifty nine mutations were found to be highly damaging to the structure and function of the HEXA gene protein. Conclusion: According to this study, thirty two novel nsSNP in HEXA are predicted to have possible role in Tay-Saches Disease using different bioinformatics tools. Our findings could help in genetic study and diagnosis of Tay-Saches Disease. Keywords: Tay-Sachs disease (TSD), GM2 gangliosidosis, hexosaminidase A, HEXA, a n...

Research paper thumbnail of The use of puppets with elementary school children in reducing stigmatizing attitudes towards mental illness

Journal of Mental Health, 2007

Method: Children received a pre and posttest of their attitudes as measured by the re-factored. O... more Method: Children received a pre and posttest of their attitudes as measured by the re-factored. Opinions About Mental Illness Scale. The experimental group watched a series of three plays in which hand puppets portrayed individuals with schizophrenia, depression/ ...