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Research paper thumbnail of Aaknox1, a kn1-like homeobox gene in Acetabularia acetabulum, undergoes developmentally regulated subcellular localization

Plant molecular biology, 1999

Homeobox-containing genes play developmentally important roles in a wide variety of plants, anima... more Homeobox-containing genes play developmentally important roles in a wide variety of plants, animals and fungi. As a way of studying how development is controlled in the unicellular green macroalga Acetabularia acetabulum, we used degenerate PCR to clone a knotted1-like (kn1-like) homeobox gene, Aaknox1 (Acetabularia acetabulum kn1-like homeobox 1). Aaknorx1 is the first knotted1-like homeobox gene to be cloned from a non-vascular plant and shows strong conservation with kn1-like genes from the vascular plants (ca. 56% amino acid identity within the homeodomain). Sequencing of cDNA clones indicates that Aaknor1 possesses at least two distinct polyadenylation sites spaced ca. 600 bp apart. Southern analysis suggests that several other kn1-like homeobox genes exist in the Acetabularia genome. Northern analyses demonstrate that expression of Aaknox1 is developmentally regulated, with peak levels of expression during early reproductive phase. Northern analyses further demonstrate that Aa...

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Research paper thumbnail of Corrigendum - Aaknox1,a kn1-like homeobox gene in Acetabularia acetabulum, undergoes developmentally regulated subcellular localization

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Research paper thumbnail of Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum

BMC plant biology, Jan 12, 2004

Acetabularia acetabulum is a giant unicellular green alga whose size and complex life cycle make ... more Acetabularia acetabulum is a giant unicellular green alga whose size and complex life cycle make it an attractive model for understanding morphogenesis and subcellular compartmentalization. The life cycle of this marine unicell is composed of several developmental phases. Juvenile and adult phases are temporally sequential but physiologically and morphologically distinct. To identify genes specific to juvenile and adult phases, we created two subtracted cDNA libraries, one adult-specific and one juvenile-specific, and analyzed 941 randomly chosen ESTs from them. Clustering analysis suggests virtually no overlap between the two libraries. Preliminary expression data also suggests that we were successful at isolating transcripts differentially expressed between the two developmental phases and that many transcripts are specific to one phase or the other. Comparison of our EST sequences against publicly available sequence databases indicates that ESTs from the adult and the juvenile li...

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Research paper thumbnail of kurkku, a Phenotype of Acetabularia acetabulum That Is Arrested in Vegetative Growth, Can Be Rescued with Wild-Type Cytoplasm

The Plant Cell, 1996

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Research paper thumbnail of Improved mating efficiency inAcetabularia acetabulum: recovery of 48?100% of the expected zygotes

Protoplasma, 1993

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Research paper thumbnail of Calcification and measurements of net proton and oxygen flux reveal subcellular domains in Acetabularia acetabulum

Planta, 2000

Vegetative adults of Acetabularia acetabulum (L.) Silva were studied as a model system for subcel... more Vegetative adults of Acetabularia acetabulum (L.) Silva were studied as a model system for subcellular patterning in plants, and a description of several phenotypic and physiological characteristics that reveal patterns of subcellular differentiation in this unicellular macroalga was undertaken. Initially, calcification patterns were studied. Under favorable conditions, the rhizoid and most of the stalk calcified. Only the apical 10-20% of the stalk and a small region adjacent to the rhizoid remained uncalcified. Calcification in algae has been reported to result from a biologically mediated local increase in alkalinity. To test this model extracellular pH and extracellular hydrogen ion gradients were examined with ion-selective, self-referencing, electrodes. In the light, A. acetabulum displayed a general pattern of extracellular alkalinity around the entire alga, although in some individuals the region near the rhizoid and the rhizoid itself displayed extracellular acidity. Acetabularia acetabulum also displayed net hydrogen ion influx at the rhizoid and the apical half of the stalk, variable flux in the lower part of the stalk, and net hydrogen ion efflux at the base of the stalk next to the rhizoid. The lack of complete correlation between external pH patterns and calcification suggests that other factors contribute to the control of calcification in this alga. To examine whether net hydrogen ion flux patterns correlated with photosynthetic or respiration patterns, oxygen flux was measured along the stalk using self-referencing O2 electrodes. Photosynthetic oxygen evolution occurred at comparable levels throughout the stalk, with less evolution in the rhizoid. Respiration mainly occurred near and in the rhizoid, with less O2 consumption occurring more apically along the stalk. Our studies of calcification patterns, net hydrogen ion flux and O2 flux revealed several overlapping patterns of subcellular differentiation in A. acetabulum.

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Research paper thumbnail of Relationships between growth, morphology and wall stress in the stalk of Acetabularia acetabulum

Planta, 2001

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Research paper thumbnail of The roles of light and the nucleus in the regulation of reproductive onset in Acetabularia acetabulum

Planta, 1999

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Research paper thumbnail of An analysis of morphogenesis of the reproductive whorl of Acetabularia acetabulum

Planta, 1998

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Research paper thumbnail of Phytochrome-Mediated Cellular Photomorphogenesis

PLANT PHYSIOLOGY, 1983

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Research paper thumbnail of Photobiology of Diagravitropic Maize Roots

PLANT PHYSIOLOGY, 1984

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Research paper thumbnail of Asymmetric Subcellular mRNA Distribution Correlates with Carbonic Anhydrase Activity in Acetabularia acetabulum

PLANT PHYSIOLOGY, 2001

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Research paper thumbnail of Development and organization of the central vacuole of Acetabularia acetabulum

New Phytologist, 2005

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Research paper thumbnail of Acetabularia: A Unicellular Model for Understanding Subcellular Localization and Morphogenesis during Development

Journal of Plant Growth Regulation, 2000

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Research paper thumbnail of Physiological Factors That Aid Differentiation of Zygotes and Early Juveniles of Acetabularia Acetabulum (Chlorophyta)

Journal of Phycology, 1999

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Research paper thumbnail of Axenic Cultures of Acetabularia (Chlorophyta): A Decontamination Protocol with Potential Application to Other ALGAE1

Journal of Phycology, 1992

... 28, 407-414 (I992) AXENIC CULTURES OF .~“lCET.~lBL-'LAR1.~l (CHLOROPHYTA): A DECONTAMlNA... more ... 28, 407-414 (I992) AXENIC CULTURES OF .~“lCET.~lBL-'LAR1.~l (CHLOROPHYTA): A DECONTAMlNATl()N PROTOCOL WITH POTENTIAL APPLICATION TO OTHER ALGAE' Bnrmlo E. Hunt' and Dino F. Mondoli Department of Botany KB-I5, University of Washington, Seattle ...

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Research paper thumbnail of A New, Artificial Sea Water That Facilitates Growth of Large Numbers of Cells of Acetabularia Acetabulum (Chlorophyta) and Reduces the Labor Inherent in Cell CULTURE1

Journal of Phycology, 1996

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Research paper thumbnail of 113Toward Resolution of the Fuzzy Nodes in Green Plant Phylogeny

Journal of Phycology, 2003

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Research paper thumbnail of The first complete chloroplast genome sequence of a lycophyte, Huperzia lucidula (Lycopodiaceae)

Gene, 2005

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Research paper thumbnail of Chloroplast genome sequence of the moss Tortula ruralis: gene content, polymorphism, and structural arrangement relative to other green plant chloroplast genomes

BMC Genomics, 2010

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Research paper thumbnail of Aaknox1, a kn1-like homeobox gene in Acetabularia acetabulum, undergoes developmentally regulated subcellular localization

Plant molecular biology, 1999

Homeobox-containing genes play developmentally important roles in a wide variety of plants, anima... more Homeobox-containing genes play developmentally important roles in a wide variety of plants, animals and fungi. As a way of studying how development is controlled in the unicellular green macroalga Acetabularia acetabulum, we used degenerate PCR to clone a knotted1-like (kn1-like) homeobox gene, Aaknox1 (Acetabularia acetabulum kn1-like homeobox 1). Aaknorx1 is the first knotted1-like homeobox gene to be cloned from a non-vascular plant and shows strong conservation with kn1-like genes from the vascular plants (ca. 56% amino acid identity within the homeodomain). Sequencing of cDNA clones indicates that Aaknor1 possesses at least two distinct polyadenylation sites spaced ca. 600 bp apart. Southern analysis suggests that several other kn1-like homeobox genes exist in the Acetabularia genome. Northern analyses demonstrate that expression of Aaknox1 is developmentally regulated, with peak levels of expression during early reproductive phase. Northern analyses further demonstrate that Aa...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Corrigendum - Aaknox1,a kn1-like homeobox gene in Acetabularia acetabulum, undergoes developmentally regulated subcellular localization

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum

BMC plant biology, Jan 12, 2004

Acetabularia acetabulum is a giant unicellular green alga whose size and complex life cycle make ... more Acetabularia acetabulum is a giant unicellular green alga whose size and complex life cycle make it an attractive model for understanding morphogenesis and subcellular compartmentalization. The life cycle of this marine unicell is composed of several developmental phases. Juvenile and adult phases are temporally sequential but physiologically and morphologically distinct. To identify genes specific to juvenile and adult phases, we created two subtracted cDNA libraries, one adult-specific and one juvenile-specific, and analyzed 941 randomly chosen ESTs from them. Clustering analysis suggests virtually no overlap between the two libraries. Preliminary expression data also suggests that we were successful at isolating transcripts differentially expressed between the two developmental phases and that many transcripts are specific to one phase or the other. Comparison of our EST sequences against publicly available sequence databases indicates that ESTs from the adult and the juvenile li...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of kurkku, a Phenotype of Acetabularia acetabulum That Is Arrested in Vegetative Growth, Can Be Rescued with Wild-Type Cytoplasm

The Plant Cell, 1996

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Improved mating efficiency inAcetabularia acetabulum: recovery of 48?100% of the expected zygotes

Protoplasma, 1993

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Calcification and measurements of net proton and oxygen flux reveal subcellular domains in Acetabularia acetabulum

Planta, 2000

Vegetative adults of Acetabularia acetabulum (L.) Silva were studied as a model system for subcel... more Vegetative adults of Acetabularia acetabulum (L.) Silva were studied as a model system for subcellular patterning in plants, and a description of several phenotypic and physiological characteristics that reveal patterns of subcellular differentiation in this unicellular macroalga was undertaken. Initially, calcification patterns were studied. Under favorable conditions, the rhizoid and most of the stalk calcified. Only the apical 10-20% of the stalk and a small region adjacent to the rhizoid remained uncalcified. Calcification in algae has been reported to result from a biologically mediated local increase in alkalinity. To test this model extracellular pH and extracellular hydrogen ion gradients were examined with ion-selective, self-referencing, electrodes. In the light, A. acetabulum displayed a general pattern of extracellular alkalinity around the entire alga, although in some individuals the region near the rhizoid and the rhizoid itself displayed extracellular acidity. Acetabularia acetabulum also displayed net hydrogen ion influx at the rhizoid and the apical half of the stalk, variable flux in the lower part of the stalk, and net hydrogen ion efflux at the base of the stalk next to the rhizoid. The lack of complete correlation between external pH patterns and calcification suggests that other factors contribute to the control of calcification in this alga. To examine whether net hydrogen ion flux patterns correlated with photosynthetic or respiration patterns, oxygen flux was measured along the stalk using self-referencing O2 electrodes. Photosynthetic oxygen evolution occurred at comparable levels throughout the stalk, with less evolution in the rhizoid. Respiration mainly occurred near and in the rhizoid, with less O2 consumption occurring more apically along the stalk. Our studies of calcification patterns, net hydrogen ion flux and O2 flux revealed several overlapping patterns of subcellular differentiation in A. acetabulum.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Relationships between growth, morphology and wall stress in the stalk of Acetabularia acetabulum

Planta, 2001

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The roles of light and the nucleus in the regulation of reproductive onset in Acetabularia acetabulum

Planta, 1999

Bookmarks Related papers MentionsView impact

Research paper thumbnail of An analysis of morphogenesis of the reproductive whorl of Acetabularia acetabulum

Planta, 1998

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Phytochrome-Mediated Cellular Photomorphogenesis

PLANT PHYSIOLOGY, 1983

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Photobiology of Diagravitropic Maize Roots

PLANT PHYSIOLOGY, 1984

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Asymmetric Subcellular mRNA Distribution Correlates with Carbonic Anhydrase Activity in Acetabularia acetabulum

PLANT PHYSIOLOGY, 2001

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Development and organization of the central vacuole of Acetabularia acetabulum

New Phytologist, 2005

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Acetabularia: A Unicellular Model for Understanding Subcellular Localization and Morphogenesis during Development

Journal of Plant Growth Regulation, 2000

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Physiological Factors That Aid Differentiation of Zygotes and Early Juveniles of Acetabularia Acetabulum (Chlorophyta)

Journal of Phycology, 1999

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Axenic Cultures of Acetabularia (Chlorophyta): A Decontamination Protocol with Potential Application to Other ALGAE1

Journal of Phycology, 1992

... 28, 407-414 (I992) AXENIC CULTURES OF .~“lCET.~lBL-'LAR1.~l (CHLOROPHYTA): A DECONTAMlNA... more ... 28, 407-414 (I992) AXENIC CULTURES OF .~“lCET.~lBL-'LAR1.~l (CHLOROPHYTA): A DECONTAMlNATl()N PROTOCOL WITH POTENTIAL APPLICATION TO OTHER ALGAE' Bnrmlo E. Hunt' and Dino F. Mondoli Department of Botany KB-I5, University of Washington, Seattle ...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of A New, Artificial Sea Water That Facilitates Growth of Large Numbers of Cells of Acetabularia Acetabulum (Chlorophyta) and Reduces the Labor Inherent in Cell CULTURE1

Journal of Phycology, 1996

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 113Toward Resolution of the Fuzzy Nodes in Green Plant Phylogeny

Journal of Phycology, 2003

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The first complete chloroplast genome sequence of a lycophyte, Huperzia lucidula (Lycopodiaceae)

Gene, 2005

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Chloroplast genome sequence of the moss Tortula ruralis: gene content, polymorphism, and structural arrangement relative to other green plant chloroplast genomes

BMC Genomics, 2010

Bookmarks Related papers MentionsView impact

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