Dinesh Dikshit - Academia.edu (original) (raw)
Papers by Dinesh Dikshit
Biochemistry, 1981
The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a... more The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a pyridoxal phosphate (PLP) linked catalyst which fragments the cyclopropane substrate to a-ketobutyrate and ammonia [Honma, M., & Shimomura, T. (1978) Agric. Biol. Chem. 42, 18251. Enzymatic incubations in D20 yield a-ketobutyrate with one deuterium at the C-4 methyl group and one deuterium at one of the C-3 prochiral methylene hydrogens. Stereochemical analysis of the location of the C-3 deuteron was accomplished by in situ enzymatic reduction to (2S)-2hydroxybutyrate with L-lactate dehydrogenase and conversion to the phenacyl ester. The C-3 hydrogens of the (2S)-2hydroxybutyryl moiety are fully resolved in a 250-MHz NMR spectrum. Absolute assignment of 3 s and 3R loci was obtained with phenacyl(2S,3S)-2-hydro~y[3-~H] butyrate generated enzymatically by D-serine dehydratase action on D-%e cyclopropanoid amino acid 1 -aminocyclopropane-1
Biochemistry, 1981
The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a... more The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a pyridoxal phosphate (PLP) linked catalyst which fragments the cyclopropane substrate to a-ketobutyrate and ammonia [Honma, M., & Shimomura, T. (1978) Agric. Biol. Chem. 42, 18251. Enzymatic incubations in D20 yield a-ketobutyrate with one deuterium at the C-4 methyl group and one deuterium at one of the C-3 prochiral methylene hydrogens. Stereochemical analysis of the location of the C-3 deuteron was accomplished by in situ enzymatic reduction to (2S)-2hydroxybutyrate with L-lactate dehydrogenase and conversion to the phenacyl ester. The C-3 hydrogens of the (2S)-2hydroxybutyryl moiety are fully resolved in a 250-MHz NMR spectrum. Absolute assignment of 3 s and 3R loci was obtained with phenacyl(2S,3S)-2-hydro~y[3-~H] butyrate generated enzymatically by D-serine dehydratase action on D-%e cyclopropanoid amino acid 1 -aminocyclopropane-1
Journal of the Chemical Society, Perkin Transactions 1, 1977
... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the f... more ... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the furanone (1) with lithium aluminium 2 DK Dikshit, K. L. Munshi, RS Kapil, and N. Anand, Indian J ... Nmr spectra were recorded with a Varian A-60D spec-trometer (Me,Si as internal reference ...
Journal of The Chemical Society-perkin Transactions 1, 1977
... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the f... more ... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the furanone (1) with lithium aluminium 2 DK Dikshit, K. L. Munshi, RS Kapil, and N. Anand, Indian J ... Nmr spectra were recorded with a Varian A-60D spec-trometer (Me,Si as internal reference ...
Tetrahedron Letters, 1999
O, C-Di-lithio di-anions derived from N-substituted pyroglutamic acids react with electrophiles t... more O, C-Di-lithio di-anions derived from N-substituted pyroglutamic acids react with electrophiles to give C-4 α- substituted products.O, C- Di-lithio di-anions derived from N-substituted pyroglutamic acids react with electrophiles to give C-4 α- substituted products.
Cytometry Part A, 2015
A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human periphe... more A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD41 cell counting including the development of higher order reference measurement procedures and has been evaluated in the pilot study CCQM-P102. This study was conducted across 16 laboratories from eight countries to assess the ability of participants to quantify the CD41 cell count of this reference material and to document cross-laboratory variability plus associated measurement uncertainties. Twelve different flow cytometer platforms were evaluated using a standard protocol that included calibration beads used to obtain quantitative measurements of CD41 T cell counts. There was good overall cross-platform and counting method agreement with a grand mean of the laboratory calculated means of (301.7 6 4.9) lL 21 CD41 cells. Excluding outliers, greater than 90% of participant data agreed within 615%. A major contribution to variation of sLL CD41 cell counts was tube to tube variation of the calibration beads, amounting to an uncertainty of 3.6%. Variation due to preparative steps equated to an uncertainty of 2.6%. There was no reduction in variability when data files were centrally reanalyzed. Remaining variation was attributed to instrument specific differences. CD41 cell counts obtained in CCQM-P102 are in excellent agreement and show the robustness of both the measurements and the data analysis and hence the suitability of sLL as a reference material for interlaboratory comparisons and external quality assessment.
Journal of Biomaterials and Tissue Engineering, 2014
A substituted 5-(piperidine-1-carbonyl) pyrrolidin-2-one (CDRI compound S007-867) is a potent ant... more A substituted 5-(piperidine-1-carbonyl) pyrrolidin-2-one (CDRI compound S007-867) is a potent antithrombotic candidate drug under development at our Institute. An HPLC method for determination of S007-867 with its intermediates has been developed and validated for in process quality control and stability studies. The analytical performance parameters such as specificity, limit of detection (LOD), lower limit of quantification (LLOQ), linearity, precision, robustness, and accuracy were determined according to International Conference on Harmonization ICH Q2(R1) guidelines. HPLC separation was achieved on C18 Lichrospheres column (250 mm, 4 mm, 5 m, Merck) using water containing 0.1% glacial acetic acid and acetonitrile as the mobile phase in an isocratic elution. The eluents were monitored by a photo diode array detector at 220 nm. Based on signal to noise ratio of 3 and 10 the LOD of S007-867 was 0.98 g/mL, while the LLOQ was 3.9 g/mL. The calibration curves were linear in the range of 3.9-125 g/mL. Precision of the method was determined by inter-and intra-assay variations within the range.
ChemInform, 2004
... Preparative Organic Chemistry. Use of S-Proline as Chiral Auxiliary in α-Alkylations of Carbo... more ... Preparative Organic Chemistry. Use of S-Proline as Chiral Auxiliary in α-Alkylations of Carboxylic Acids. Stuti Srivastava,; Lalit N. Goswami,; Dinesh K. Dikshit. Article first published online: 8 JAN 2004. DOI: 10.1002/chin.200404025. ...
ChemInform, 2009
ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Tetrahedron Letters, 1995
Journal of Medicinal Chemistry, 1989
A series of 2,3-diaryl-1-benzopyran analogues substituted at position 4 of 2-phenyl with a hydrox... more A series of 2,3-diaryl-1-benzopyran analogues substituted at position 4 of 2-phenyl with a hydroxy or pyrrolidinoethoxy residue were synthesized as models for (E)-triarylpropenones constrained in the s-trans conformation. The prototypes, belonging to five chemical series, were evaluated for their estrogen receptor affinity and for estrogen agonist-antagonist activities. The 4H-1-benzopyran-4-one, the 2,3-dihydro-4H-1-benzopyran-4-one, the 4H-1-benzopyran, and the 2,3-dihydro-1-benzopyran derivatives were found to be inactive or only marginally activate as receptor ligands or estrogen agonists-antagonists. In the 2H-1-benzopyran category the parent phenol was also inactive whereas the basic ethers 16 and 26 were modest receptor ligands while being quite active as antiestrogens. In a comparative study the benzopyran 16 was found to be more effective antiestrogen than tamoxifen while being as effective as LY-117018. The benzopyrans have thus emerged as a new class of potent antiestrogens.
Cheminform, 1991
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Journal of Organic Chemistry, 1980
Tetrahedron Letters, 1995
... KVR; Roy, R.; Singh,J.; Anand,N. Tetrahedron 1992,48, 4985. 4. (a) Ezquerra, J.; Pedregal, C.... more ... KVR; Roy, R.; Singh,J.; Anand,N. Tetrahedron 1992,48, 4985. 4. (a) Ezquerra, J.; Pedregal, C.; Rubio, A.; Yruretagoyena, B.; Escribano, A.; Ferrando, ES. Tetrahedron 1993, 49, 8665. (b) Dikshit DK; Panday, SK ]. Org. Chem. 1992, 57, 1920. (c) Baldwin, JE; Miranda, T.; Moloney ...
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Tetrahedron, 2011
Aza variant of intramolecular catalytic, asymmetric nucleophile-catalyzed, aldol lactonization (N... more Aza variant of intramolecular catalytic, asymmetric nucleophile-catalyzed, aldol lactonization (NCAL) reaction has been explored to synthesize β-lactone fused nitrogen heterocycles as aza sugars’ precursors by employing achiral amino acids. The utility of these bicyclic β-lactones is presented by the formal synthesis of aza sugars, (3S,4R) and (3R,4S) 4-(hydroxymethyl)pyrrolidin-3-ol.
Synlett, 2003
LETTER, Synlett 2003(11): 1737-1739 DOI: 10.1055/s-2003-41433. © Georg Thieme Verlag Stuttgart · ... more LETTER, Synlett 2003(11): 1737-1739 DOI: 10.1055/s-2003-41433. © Georg Thieme Verlag Stuttgart · New York. A Short Synthesis of Preussin: Use of Allyldimethylsilyl as Masked Hydroxyl [1]. Dinesh K. Dikshit*, Lalit N. Goswami, Vishnu S. Singh. ...
Indian Journal of Public Health Research & Development, 2014
ChemInform, 2003
Skip to Main Content. ...
Biochemistry, 1981
The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a... more The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a pyridoxal phosphate (PLP) linked catalyst which fragments the cyclopropane substrate to a-ketobutyrate and ammonia [Honma, M., & Shimomura, T. (1978) Agric. Biol. Chem. 42, 18251. Enzymatic incubations in D20 yield a-ketobutyrate with one deuterium at the C-4 methyl group and one deuterium at one of the C-3 prochiral methylene hydrogens. Stereochemical analysis of the location of the C-3 deuteron was accomplished by in situ enzymatic reduction to (2S)-2hydroxybutyrate with L-lactate dehydrogenase and conversion to the phenacyl ester. The C-3 hydrogens of the (2S)-2hydroxybutyryl moiety are fully resolved in a 250-MHz NMR spectrum. Absolute assignment of 3 s and 3R loci was obtained with phenacyl(2S,3S)-2-hydro~y[3-~H] butyrate generated enzymatically by D-serine dehydratase action on D-%e cyclopropanoid amino acid 1 -aminocyclopropane-1
Biochemistry, 1981
The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a... more The enzyme 1 -aminocyclopropane-1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a pyridoxal phosphate (PLP) linked catalyst which fragments the cyclopropane substrate to a-ketobutyrate and ammonia [Honma, M., & Shimomura, T. (1978) Agric. Biol. Chem. 42, 18251. Enzymatic incubations in D20 yield a-ketobutyrate with one deuterium at the C-4 methyl group and one deuterium at one of the C-3 prochiral methylene hydrogens. Stereochemical analysis of the location of the C-3 deuteron was accomplished by in situ enzymatic reduction to (2S)-2hydroxybutyrate with L-lactate dehydrogenase and conversion to the phenacyl ester. The C-3 hydrogens of the (2S)-2hydroxybutyryl moiety are fully resolved in a 250-MHz NMR spectrum. Absolute assignment of 3 s and 3R loci was obtained with phenacyl(2S,3S)-2-hydro~y[3-~H] butyrate generated enzymatically by D-serine dehydratase action on D-%e cyclopropanoid amino acid 1 -aminocyclopropane-1
Journal of the Chemical Society, Perkin Transactions 1, 1977
... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the f... more ... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the furanone (1) with lithium aluminium 2 DK Dikshit, K. L. Munshi, RS Kapil, and N. Anand, Indian J ... Nmr spectra were recorded with a Varian A-60D spec-trometer (Me,Si as internal reference ...
Journal of The Chemical Society-perkin Transactions 1, 1977
... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the f... more ... the present data it is not possible to assign the stereo-chemistry at C-2. Reduction of the furanone (1) with lithium aluminium 2 DK Dikshit, K. L. Munshi, RS Kapil, and N. Anand, Indian J ... Nmr spectra were recorded with a Varian A-60D spec-trometer (Me,Si as internal reference ...
Tetrahedron Letters, 1999
O, C-Di-lithio di-anions derived from N-substituted pyroglutamic acids react with electrophiles t... more O, C-Di-lithio di-anions derived from N-substituted pyroglutamic acids react with electrophiles to give C-4 α- substituted products.O, C- Di-lithio di-anions derived from N-substituted pyroglutamic acids react with electrophiles to give C-4 α- substituted products.
Cytometry Part A, 2015
A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human periphe... more A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD41 cell counting including the development of higher order reference measurement procedures and has been evaluated in the pilot study CCQM-P102. This study was conducted across 16 laboratories from eight countries to assess the ability of participants to quantify the CD41 cell count of this reference material and to document cross-laboratory variability plus associated measurement uncertainties. Twelve different flow cytometer platforms were evaluated using a standard protocol that included calibration beads used to obtain quantitative measurements of CD41 T cell counts. There was good overall cross-platform and counting method agreement with a grand mean of the laboratory calculated means of (301.7 6 4.9) lL 21 CD41 cells. Excluding outliers, greater than 90% of participant data agreed within 615%. A major contribution to variation of sLL CD41 cell counts was tube to tube variation of the calibration beads, amounting to an uncertainty of 3.6%. Variation due to preparative steps equated to an uncertainty of 2.6%. There was no reduction in variability when data files were centrally reanalyzed. Remaining variation was attributed to instrument specific differences. CD41 cell counts obtained in CCQM-P102 are in excellent agreement and show the robustness of both the measurements and the data analysis and hence the suitability of sLL as a reference material for interlaboratory comparisons and external quality assessment.
Journal of Biomaterials and Tissue Engineering, 2014
A substituted 5-(piperidine-1-carbonyl) pyrrolidin-2-one (CDRI compound S007-867) is a potent ant... more A substituted 5-(piperidine-1-carbonyl) pyrrolidin-2-one (CDRI compound S007-867) is a potent antithrombotic candidate drug under development at our Institute. An HPLC method for determination of S007-867 with its intermediates has been developed and validated for in process quality control and stability studies. The analytical performance parameters such as specificity, limit of detection (LOD), lower limit of quantification (LLOQ), linearity, precision, robustness, and accuracy were determined according to International Conference on Harmonization ICH Q2(R1) guidelines. HPLC separation was achieved on C18 Lichrospheres column (250 mm, 4 mm, 5 m, Merck) using water containing 0.1% glacial acetic acid and acetonitrile as the mobile phase in an isocratic elution. The eluents were monitored by a photo diode array detector at 220 nm. Based on signal to noise ratio of 3 and 10 the LOD of S007-867 was 0.98 g/mL, while the LLOQ was 3.9 g/mL. The calibration curves were linear in the range of 3.9-125 g/mL. Precision of the method was determined by inter-and intra-assay variations within the range.
ChemInform, 2004
... Preparative Organic Chemistry. Use of S-Proline as Chiral Auxiliary in α-Alkylations of Carbo... more ... Preparative Organic Chemistry. Use of S-Proline as Chiral Auxiliary in α-Alkylations of Carboxylic Acids. Stuti Srivastava,; Lalit N. Goswami,; Dinesh K. Dikshit. Article first published online: 8 JAN 2004. DOI: 10.1002/chin.200404025. ...
ChemInform, 2009
ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Tetrahedron Letters, 1995
Journal of Medicinal Chemistry, 1989
A series of 2,3-diaryl-1-benzopyran analogues substituted at position 4 of 2-phenyl with a hydrox... more A series of 2,3-diaryl-1-benzopyran analogues substituted at position 4 of 2-phenyl with a hydroxy or pyrrolidinoethoxy residue were synthesized as models for (E)-triarylpropenones constrained in the s-trans conformation. The prototypes, belonging to five chemical series, were evaluated for their estrogen receptor affinity and for estrogen agonist-antagonist activities. The 4H-1-benzopyran-4-one, the 2,3-dihydro-4H-1-benzopyran-4-one, the 4H-1-benzopyran, and the 2,3-dihydro-1-benzopyran derivatives were found to be inactive or only marginally activate as receptor ligands or estrogen agonists-antagonists. In the 2H-1-benzopyran category the parent phenol was also inactive whereas the basic ethers 16 and 26 were modest receptor ligands while being quite active as antiestrogens. In a comparative study the benzopyran 16 was found to be more effective antiestrogen than tamoxifen while being as effective as LY-117018. The benzopyrans have thus emerged as a new class of potent antiestrogens.
Cheminform, 1991
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Journal of Organic Chemistry, 1980
Tetrahedron Letters, 1995
... KVR; Roy, R.; Singh,J.; Anand,N. Tetrahedron 1992,48, 4985. 4. (a) Ezquerra, J.; Pedregal, C.... more ... KVR; Roy, R.; Singh,J.; Anand,N. Tetrahedron 1992,48, 4985. 4. (a) Ezquerra, J.; Pedregal, C.; Rubio, A.; Yruretagoyena, B.; Escribano, A.; Ferrando, ES. Tetrahedron 1993, 49, 8665. (b) Dikshit DK; Panday, SK ]. Org. Chem. 1992, 57, 1920. (c) Baldwin, JE; Miranda, T.; Moloney ...
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Cheminform, 2010
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Tetrahedron, 2011
Aza variant of intramolecular catalytic, asymmetric nucleophile-catalyzed, aldol lactonization (N... more Aza variant of intramolecular catalytic, asymmetric nucleophile-catalyzed, aldol lactonization (NCAL) reaction has been explored to synthesize β-lactone fused nitrogen heterocycles as aza sugars’ precursors by employing achiral amino acids. The utility of these bicyclic β-lactones is presented by the formal synthesis of aza sugars, (3S,4R) and (3R,4S) 4-(hydroxymethyl)pyrrolidin-3-ol.
Synlett, 2003
LETTER, Synlett 2003(11): 1737-1739 DOI: 10.1055/s-2003-41433. © Georg Thieme Verlag Stuttgart · ... more LETTER, Synlett 2003(11): 1737-1739 DOI: 10.1055/s-2003-41433. © Georg Thieme Verlag Stuttgart · New York. A Short Synthesis of Preussin: Use of Allyldimethylsilyl as Masked Hydroxyl [1]. Dinesh K. Dikshit*, Lalit N. Goswami, Vishnu S. Singh. ...
Indian Journal of Public Health Research & Development, 2014
ChemInform, 2003
Skip to Main Content. ...