Dr. Vinayagamoorthy nadimuthu - Academia.edu (original) (raw)
Uploads
Papers by Dr. Vinayagamoorthy nadimuthu
ALGAE, 2012
Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacter... more Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacteria especially in the proper functioning of Rubisco and assimilation of carbon via the Calvin cycle. This study evaluates the role of carbon dioxide on carbon concentrating mechanism (CCM) in a cynaobacteria, Spirulina platensis and a microalga, Chlorella sp. 786. The study organisms were grown in both atmospheric (control sample, 0.035%) and high (exposed sample, 10%) CO 2 concentrations. Second dimension (2D) electrophoresis revealed a huge difference in the protein profiles of both organisms suggesting the induction of CCM related proteins in the sample maintained at atmospheric CO 2 concentration and the repression of CCM related proteins in the sample maintained at 10% CO 2. Liquid chromatography-mass spectroscopy analysis revealed the presence of two important C i transporter proteins in the control sample of S. platensis, namely ferredoxin-NADP + reductase and ATP binding cassette (ABC) transport system protein. These proteins were only expressed in the control sample and were downregulated or not expressed at all in the exposed sample. Consequently, this study conclusively proves that CCMs are only inducted at low CO 2 concentrations and are not functional at high CO 2 concentration.
PLoS ONE, 2014
C-reactive protein (CRP) is a general marker of systemic inflammation and cardiovascular disease ... more C-reactive protein (CRP) is a general marker of systemic inflammation and cardiovascular disease (CVD). The genetic contribution to differences in CRP levels remains to be explained, especially in non-European populations. Thus, the aim of this study was to identify genetic loci associated with CRP levels in Korean population. We performed genome-wide association studies (GWAS) using SNPs from 8,529 Korean individuals (7,626 for stage 1 and 903 for stage 2). We also performed pathway analysis. We identified a new genetic locus associated with CRP levels upstream of ARG1 gene (top significant SNP: rs9375813, P meta = 2.85610 28), which encodes a key enzyme of the urea cycle counteract the effects of nitric oxide, in addition to known CRP (rs7553007, P meta = 1.72610 216) and HNF1A loci (rs2259816, P meta = 2.90610 210). When we evaluated the associations between the CRP-related SNPs with cardiovascular disease phenotypes, rs9375813 (ARG1) showed a marginal association with hypertension (P = 0.0440). To identify more variants and pathways, we performed pathway analysis and identified six candidate pathways comprised of genes related to inflammatory processes and CVDs (CRP, HNF1A, PCSK6, CD36, and ABCA1). In addition to the previously reported loci (CRP, HNF1A, and IL6) in diverse ethnic groups, we identified novel variants in the ARG1 locus associated with CRP levels in Korean population and a number of interesting genes related to inflammatory processes and CVD through pathway analysis.
Journal of Occupational and Environmental Medicine, 2007
To assess the oxidative stress induced due to heavy metal exposure. Exposed populations are selec... more To assess the oxidative stress induced due to heavy metal exposure. Exposed populations are selected from an engine tuning station and control from the same area with no occupational exposure. Method: Standard methods were followed for enzymatic assay, and heavy metals in blood and urine were analyzed by using inductively coupled plasma-optical emission spectrophotometer after microwave digestion. Result: Changes in mean blood Pb, Cd, and Ni concentrations in blood and urine of exposed population of all age groups (20 to 35, 35 to 45, and 46 to 58 years) and exposure durations (Յ10, 11 to 20, and Ͼ20 years) were statistically not significant. However, exposed workers exhibited statistically significant higher antioxidant status in terms of serum glutathione-S-transferase activity, malondialdehyde level, and catalase activity. Conclusion: The findings in this article suggest that occupational exposure to diesel exhaust of engine tuning workers causes induction of oxidative stress, which cannot be correlated with the heavy metals status in blood and urine of an exposed population.
Chemosphere, 2010
Manganese (Mn) intoxication is most often regarded as an occupational manifestation and occurs in... more Manganese (Mn) intoxication is most often regarded as an occupational manifestation and occurs in places such as manganese mines, dry cell battery plants and ceramic industries. In the present study, the influence of genetic polymorphism in cytochrome P450 2D6 (CYP2D6*2), glutathione S-transferase M1 (GSTM1) and NAD(P)H quinone oxidoreductase 1 (NQO1) genes on blood manganese and plasma prolactin concentrations in manganese miners was investigated. Genotyping of CYP2D6*2 C ? T 2850 and NQO1 C ? T 609 was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) whereas the genotyping of GSTM1 was carried out by multiplex PCR using beta globin as an internal control. Manganese miners with CYP2D6*2 C ? T 2850 variant genotype had relatively low Mn concentration [GM: 21.4 ± 8.9 lg L À1 ] than the subjects with wild (GM: 36.3 ± 8.5 lg L À1) and heterozygous (GM: 34.4 ± 6.9 lg L À1) genotypes. Miners with CYP2D6*2 variant genotypes showed low prolactin levels (GM: 13.13 ± 1.6 ng mL À1) compared to the wild (GM: 16.4.4 ± 1.5 lg L À1) and heterozygous (GM: 18.7 ± 1.6 ng mL À1) genotypes. Gene-gene interaction studies also revealed that the subjects with CYP2D6*2 C ? T 2850 variant genotypes had low levels of Mn and prolactin. Our new findings suggest that CYP2D6*2 C ? T 2850 variant genotypes can regulate plasma prolactin levels in manganese miners of Central India and could be involved in the fast metabolism of blood manganese, compared to wild and heterozygous genotypes.
Biotechnology and Bioprocess Engineering, 2009
Carbonic anhydrase enzyme, one of the fastest known enzymes, remains largely unexplored in prokar... more Carbonic anhydrase enzyme, one of the fastest known enzymes, remains largely unexplored in prokaryotes when compared to its mammalian counterparts despite its ubiquity. In this study, the enzyme has been purified from Bacillus subtilis SA3 using sequential Sephadex G-75 chromatography, DEAE cellulose chromatography, and sepharose-4B-L-tyrosinesulphanilamide affinity chromatography and characterized to provide additional insights into its properties. The apparent molecular mass of carbonic anhydrase obtained by SDS-PAGE was found to be approximately 37 kDa. Isoelectric focusing of the purified enzyme revealed an isoelectric point (pI) of around 6.1 when compared with marker. The presence of metal ions such as Zn 2+ , Co 2+ , Cu 2+ , Fe 3+ , Mg 2+ , and anion SO 4 − increased enzyme activity while strong inhibition was observed in the presence of Hg 2+ , Cl − , HCO 3 − , and metal chelator EDTA. The optimum pH and temperature for the enzyme were found to be 8.3 and 37°C, respectively. Enzyme kinetics with p-nitrophenyl acetate as substrate at pH 8.3 and 37°C determined the V max and K m values of the enzyme to be 714.28 µmol/mg protein/min and 9.09 mM, respectively. The K i value for acetazolamide was 0.22 mM, compared to 0.099 mM for sulphanilamide. The results from N-terminal amino acid sequencing imply the purified protein is a putative beta-carbonic anhydrase with close similarities to CAs from plants, microorganisms. © KSBB
ALGAE, 2012
Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacter... more Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacteria especially in the proper functioning of Rubisco and assimilation of carbon via the Calvin cycle. This study evaluates the role of carbon dioxide on carbon concentrating mechanism (CCM) in a cynaobacteria, Spirulina platensis and a microalga, Chlorella sp. 786. The study organisms were grown in both atmospheric (control sample, 0.035%) and high (exposed sample, 10%) CO 2 concentrations. Second dimension (2D) electrophoresis revealed a huge difference in the protein profiles of both organisms suggesting the induction of CCM related proteins in the sample maintained at atmospheric CO 2 concentration and the repression of CCM related proteins in the sample maintained at 10% CO 2. Liquid chromatography-mass spectroscopy analysis revealed the presence of two important C i transporter proteins in the control sample of S. platensis, namely ferredoxin-NADP + reductase and ATP binding cassette (ABC) transport system protein. These proteins were only expressed in the control sample and were downregulated or not expressed at all in the exposed sample. Consequently, this study conclusively proves that CCMs are only inducted at low CO 2 concentrations and are not functional at high CO 2 concentration.
PLoS ONE, 2014
C-reactive protein (CRP) is a general marker of systemic inflammation and cardiovascular disease ... more C-reactive protein (CRP) is a general marker of systemic inflammation and cardiovascular disease (CVD). The genetic contribution to differences in CRP levels remains to be explained, especially in non-European populations. Thus, the aim of this study was to identify genetic loci associated with CRP levels in Korean population. We performed genome-wide association studies (GWAS) using SNPs from 8,529 Korean individuals (7,626 for stage 1 and 903 for stage 2). We also performed pathway analysis. We identified a new genetic locus associated with CRP levels upstream of ARG1 gene (top significant SNP: rs9375813, P meta = 2.85610 28), which encodes a key enzyme of the urea cycle counteract the effects of nitric oxide, in addition to known CRP (rs7553007, P meta = 1.72610 216) and HNF1A loci (rs2259816, P meta = 2.90610 210). When we evaluated the associations between the CRP-related SNPs with cardiovascular disease phenotypes, rs9375813 (ARG1) showed a marginal association with hypertension (P = 0.0440). To identify more variants and pathways, we performed pathway analysis and identified six candidate pathways comprised of genes related to inflammatory processes and CVDs (CRP, HNF1A, PCSK6, CD36, and ABCA1). In addition to the previously reported loci (CRP, HNF1A, and IL6) in diverse ethnic groups, we identified novel variants in the ARG1 locus associated with CRP levels in Korean population and a number of interesting genes related to inflammatory processes and CVD through pathway analysis.
Journal of Occupational and Environmental Medicine, 2007
To assess the oxidative stress induced due to heavy metal exposure. Exposed populations are selec... more To assess the oxidative stress induced due to heavy metal exposure. Exposed populations are selected from an engine tuning station and control from the same area with no occupational exposure. Method: Standard methods were followed for enzymatic assay, and heavy metals in blood and urine were analyzed by using inductively coupled plasma-optical emission spectrophotometer after microwave digestion. Result: Changes in mean blood Pb, Cd, and Ni concentrations in blood and urine of exposed population of all age groups (20 to 35, 35 to 45, and 46 to 58 years) and exposure durations (Յ10, 11 to 20, and Ͼ20 years) were statistically not significant. However, exposed workers exhibited statistically significant higher antioxidant status in terms of serum glutathione-S-transferase activity, malondialdehyde level, and catalase activity. Conclusion: The findings in this article suggest that occupational exposure to diesel exhaust of engine tuning workers causes induction of oxidative stress, which cannot be correlated with the heavy metals status in blood and urine of an exposed population.
Chemosphere, 2010
Manganese (Mn) intoxication is most often regarded as an occupational manifestation and occurs in... more Manganese (Mn) intoxication is most often regarded as an occupational manifestation and occurs in places such as manganese mines, dry cell battery plants and ceramic industries. In the present study, the influence of genetic polymorphism in cytochrome P450 2D6 (CYP2D6*2), glutathione S-transferase M1 (GSTM1) and NAD(P)H quinone oxidoreductase 1 (NQO1) genes on blood manganese and plasma prolactin concentrations in manganese miners was investigated. Genotyping of CYP2D6*2 C ? T 2850 and NQO1 C ? T 609 was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) whereas the genotyping of GSTM1 was carried out by multiplex PCR using beta globin as an internal control. Manganese miners with CYP2D6*2 C ? T 2850 variant genotype had relatively low Mn concentration [GM: 21.4 ± 8.9 lg L À1 ] than the subjects with wild (GM: 36.3 ± 8.5 lg L À1) and heterozygous (GM: 34.4 ± 6.9 lg L À1) genotypes. Miners with CYP2D6*2 variant genotypes showed low prolactin levels (GM: 13.13 ± 1.6 ng mL À1) compared to the wild (GM: 16.4.4 ± 1.5 lg L À1) and heterozygous (GM: 18.7 ± 1.6 ng mL À1) genotypes. Gene-gene interaction studies also revealed that the subjects with CYP2D6*2 C ? T 2850 variant genotypes had low levels of Mn and prolactin. Our new findings suggest that CYP2D6*2 C ? T 2850 variant genotypes can regulate plasma prolactin levels in manganese miners of Central India and could be involved in the fast metabolism of blood manganese, compared to wild and heterozygous genotypes.
Biotechnology and Bioprocess Engineering, 2009
Carbonic anhydrase enzyme, one of the fastest known enzymes, remains largely unexplored in prokar... more Carbonic anhydrase enzyme, one of the fastest known enzymes, remains largely unexplored in prokaryotes when compared to its mammalian counterparts despite its ubiquity. In this study, the enzyme has been purified from Bacillus subtilis SA3 using sequential Sephadex G-75 chromatography, DEAE cellulose chromatography, and sepharose-4B-L-tyrosinesulphanilamide affinity chromatography and characterized to provide additional insights into its properties. The apparent molecular mass of carbonic anhydrase obtained by SDS-PAGE was found to be approximately 37 kDa. Isoelectric focusing of the purified enzyme revealed an isoelectric point (pI) of around 6.1 when compared with marker. The presence of metal ions such as Zn 2+ , Co 2+ , Cu 2+ , Fe 3+ , Mg 2+ , and anion SO 4 − increased enzyme activity while strong inhibition was observed in the presence of Hg 2+ , Cl − , HCO 3 − , and metal chelator EDTA. The optimum pH and temperature for the enzyme were found to be 8.3 and 37°C, respectively. Enzyme kinetics with p-nitrophenyl acetate as substrate at pH 8.3 and 37°C determined the V max and K m values of the enzyme to be 714.28 µmol/mg protein/min and 9.09 mM, respectively. The K i value for acetazolamide was 0.22 mM, compared to 0.099 mM for sulphanilamide. The results from N-terminal amino acid sequencing imply the purified protein is a putative beta-carbonic anhydrase with close similarities to CAs from plants, microorganisms. © KSBB