Dominika Ďurechová - Academia.edu (original) (raw)
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Papers by Dominika Ďurechová
Round-leaf sundew (Drosera rotundifolia L.) from Droseraceae family belongs among a few plant spe... more Round-leaf sundew (Drosera rotundifolia L.) from Droseraceae family belongs among a few plant species with strong antifungal potential. It was previously shown that chitinases of carnivorous plant species may play role during the insect prey digestion, when hard chitin skeleton is being decomposed. As many phytopathogenic fungi contain chitin in their cell wall our attention in this work was focused on isolation and in silico characterization of genomic DNA sequence of sundew chitinase gene. Subsequently this gene was fused to strong constitutive CaMV35S promoter and cloned into the plant binary vector pBinPlus and tested in A. tumefaciens LBA 4404 for its stability. Next, when transgenic tobacco plants are obtained, increasing of their antifungal potential will be tested.
Droseracae spp. is widely used in folk medicine. In the present study, the antimicrobial activiti... more Droseracae spp. is widely used in folk medicine. In the present study, the antimicrobial activities of the four Drosera rotundifolia L. (D8.11, D15.12, 18.10, 8.11) samples were investigated. The antimicrobial activities were determined by using agar disc diffusion method against grampositive bacteria ( Bacillus thurigiensis, Staphylococcus aureus, Listeria monocytogenes ) and gramnegative bacteria ( Yersinia enterocolitica, Salmonella enteritidis ). The results of the disk diffusion method showed very different activity against all tested strains of microorganisms. The best antimicrobial activity of ethanolic extract Drosera rotundifolia L. against Salmonella enteritidis was found at Drosera rotundifolia (D8.11).
Journal of Microbiology, Biotechnology and Food Sciences, Feb 1, 2016
Journal of Microbiology, Biotechnology and Food Sciences, Feb 2, 2015
Round-leaf sundew (Drosera rotundifolia L.) from Droseraceae family belongs among a few plant spe... more Round-leaf sundew (Drosera rotundifolia L.) from Droseraceae family belongs among a few plant species with strong antifungal potential. It was previously shown that chitinases of carnivorous plant species may play role during the insect prey digestion, when hard chitin skeleton is being decomposed. As many phytopathogenic fungi contain chitin in their cell wall our attention in this work was focused on isolation and in silico characterization of genomic DNA sequence of sundew chitinase gene. Subsequently this gene was fused to strong constitutive CaMV35S promoter and cloned into the plant binary vector pBinPlus and tested in A. tumefaciens LBA 4404 for its stability. Next, when transgenic tobacco plants are obtained, increasing of their antifungal potential will be tested.
Droseracae spp. is widely used in folk medicine. In the present study, the antimicrobial activiti... more Droseracae spp. is widely used in folk medicine. In the present study, the antimicrobial activities of the four Drosera rotundifolia L. (D8.11, D15.12, 18.10, 8.11) samples were investigated. The antimicrobial activities were determined by using agar disc diffusion method against grampositive bacteria ( Bacillus thurigiensis, Staphylococcus aureus, Listeria monocytogenes ) and gramnegative bacteria ( Yersinia enterocolitica, Salmonella enteritidis ). The results of the disk diffusion method showed very different activity against all tested strains of microorganisms. The best antimicrobial activity of ethanolic extract Drosera rotundifolia L. against Salmonella enteritidis was found at Drosera rotundifolia (D8.11).
Journal of Microbiology, Biotechnology and Food Sciences, Feb 1, 2016
Journal of Microbiology, Biotechnology and Food Sciences, Feb 2, 2015