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Papers by Dong Wan Lee

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009

A novel, Gram-negative bacterium, designated CS5-B2 T , was isolated from soil that had been coll... more A novel, Gram-negative bacterium, designated CS5-B2 T , was isolated from soil that had been collected from a cliff on Mara Island, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the radiation of the genus Dyella. The closest relatives were the type strains of Dyella koreensis, Dyella ginsengisoli and Dyella japonica and 16S rRNA gene sequence similarities between strain CS5-B2 T and these strains were 99.0, 97.9 and 97.8 %, respectively. The sequence similarities between the novel isolate and other related taxa compared in the phylogenetic analysis were less than 96.7 %. The cells of strain CS5-B2 T were aerobic, oxidase-negative, catalase-positive, motile rods. The temperature range for growth was 20-37 6C, with optimal growth at 30-37 6C. Growth occurred at pH 5.1-9.1, with optimal growth at pH 6.1-9.1. NaCl tolerance for growth was from 1 to 2 % (w/v). Ubiquinone-8 was the predominant respiratory lipoquinone. The major fatty acids were iso-C 15 : 0 and iso-C 17 : 1 v9c. The G+C content of the DNA was 65.7-66.6 mol%. The level of DNA-DNA relatedness with D. koreensis KCTC 12359 T was 20.2 and 29.6 % in duplicate measurements. On the basis of phenotypic features, phylogenetic analysis and DNA-DNA relatedness, a novel species of the genus Dyella is proposed, with the name Dyella marensis sp. nov. The type strain is CS5-B2 T (5JCM 14959 T 5KCTC 22144 T).

International journal of systematic and evolutionary microbiology, Jan 31, 2017

A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isola... more A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2T belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were 'Echinicola shivajiensis' AK12T (98.1 % 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754T (96.4 %), Echinicola jeungdonensis HMD 3054T (96.0 %), Echinicola pacifica KMM 6172T (96.0 %) and Echinicola rosea JL 3085T (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω6c and summed feature 9 (comprising iso-C17 : 1...

PLOS ONE, 2019

Probiotics are dietary supplements containing viable, non-pathogenic microorganisms that interact... more Probiotics are dietary supplements containing viable, non-pathogenic microorganisms that interact with the gastrointestinal microflora and directly with the immune system. The possible health effects of probiotics include modulating the immune system and exerting antibacterial, anticancer, and anti-mutagenic effects. The purpose of this study was to isolate, identify, and characterize novel strains of probiotics from the faeces of Korean infants. Various assays were conducted to determine the physiological features of candidate probiotic isolates, including Gram staining, 16S rRNA gene sequencing, tolerance assays to stimulated gastric juice and bile salts, adherence ability assays, antibiotic susceptibility testing, and assays of immunomodulatory effects. Based on these morphological and biochemical characteristics, five potential probiotic isolates (Enterococcus faecalis BioE EF71, Lactobacillus fermentum BioE LF11, Lactobacillus plantarum BioE LPL59, Lactobacillus paracasei BioE LP08, and Streptococcus thermophilus BioE ST107) were selected. E. faecalis BioE EF71 and L. plantarum BioE LPL59 showed high tolerance to stimulated gastric juice and bile salts, and S. thermophilus BioE ST107 as well as these two strains exhibited stronger adherence ability than reference strain Lactobacillus rhamnosus GG. All five strains inhibited secretion of lipopolysaccharide-induced pro-inflammatory cytokines IL-6 and TNF-α in RAW264.7 macrophages in vitro. L. fermentum BioE LF11, L. plantarum BioE LPL59, and S. thermophilus BioE ST107 enhanced the production of anti-inflammatory cytokine IL-10. Overall, our findings demonstrate that the five novel strains have potential as safe probiotics and encouraged varying degrees of immunomodulatory effects.

International Journal of Systematic and Evolutionary Microbiology, 2018

A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8, was isolate... more A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea. Cells were aerobic, catalase-negative, oxidase-positive, non-motile and cocci, occurring singly, in pairs or in tetrads, and often tending to form aggregates. The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %). Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Whole-cell sugar analysis of strain GP-S2-8 revealed rhamnose, glucose and mannose as characteristic sugars. The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0. The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content was 74.2 mol%. DNA-DNA relatedness values between strain GP-S2-8 and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %. On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp. nov. is proposed. The type strain is GP-S2-8 (=KCCM 43275=JCM 32354=DSM 106127=KCTC 49078).

International Journal of Systematic and Evolutionary Microbiology, 2018

A novel Gram-stain-negative, moderately halophilic and aerobic bacterium, designated strain SDRB-... more A novel Gram-stain-negative, moderately halophilic and aerobic bacterium, designated strain SDRB-Phe2, was isolated from coastal sediment of the yellow sea in Sindu-ri, Republic of Korea. Cells were oxidase-positive, catalase-positive, rod-shaped and surrounded by a capsule with gliding motility. Colonies were yellow-coloured, circular, pulvinate with entire margins. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SDRB-Phe2 formed a distinct lineage within the genus Maribacter of the family Flavobacteriaceae. Stain SDRB-Phe2 exhibited 16S rRNA gene sequence similarity values of 97.1-98.9 % to the type strains of Maribacterstanieri, Maribacterspongiicola, Maribacter forsetii, Maribacter dokdonensis, Maribacter aquivivus, Maribactercaenipelagi, Maribacterlitorisediminis, Maribactersedimenticola, Maribacterulvicola, Maribacter confluentis and Maribacter orientalis, and of 94.8-96.7 % to the type strains of the other species of the genus Maribacter. Strain SDRB-Phe2 contained MK-6 as the predominant respiratory quinone and iso-C15 : 1 G, iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The polar lipids of strain SDRB-Phe2 were phosphatidylethanolamine, one unidentified amino lipid and two unidentified lipids. The DNA G+C content was 36.2 mol%. DNA-DNA relatedness values of strain SDRB-Phe2 to the type strains of the 11 phylogenetically related species of the genus Maribacter were 21.9-38.6 %. On the basis of the phenotypic features, phylogenetic and DNA-DNA hybridization analyses presented here, strain SDRB-Phe2 (=JCM 32373=KCTC 62273=DSM 106042) represents a novel species of the genus Maribacter, for which the name Maribacterlitoralis sp. nov. is proposed.

Environmental pollution (Barking, Essex : 1987), 2018

Crude oil and its derivatives are considered as one group of the most pervasive environmental pol... more Crude oil and its derivatives are considered as one group of the most pervasive environmental pollutants in marine environments. Bioremediation using oil-degrading bacteria has emerged as a promising green cleanup alternative in more recent years. The employment of biosurfactant-producing and hydrocarbon-utilizing indigenous bacteria enhances the effectiveness of bioremediation by making hydrocarbons bioavailable for degradation. In this study, the best candidates of biosurfactant-producing indigenous bacteria were selected by screening of biochemical tests. The selected bacteria include Bacillus algicola (003-Phe1), Rhodococcus soli (102-Na5), Isoptericola chiayiensis (103-Na4), and Pseudoalteromonas agarivorans (SDRB-Py1). In general, these isolated species caused low surface tension values (33.9-41.3 mN m), high oil spreading (1.2-2.4 cm), and hydrocarbon emulsification (up to 65%) warranting active degradation of hydrocarbons. FT-IR and LC-MS analyses indicated that the monorham...

International journal of systematic and evolutionary microbiology, Jan 24, 2018

A Gram-stain-negative, strictly aerobic, motile and rod-shaped bacterial strain, designated 102-N... more A Gram-stain-negative, strictly aerobic, motile and rod-shaped bacterial strain, designated 102-Na3, was isolated from sediment of Sinduri beach in Taean, Republic of Korea. Strain 102-Na3 grew optimally at 28-37 °C, at pH 7.0-11.0 and in the presence of 1-3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain 102-Na3 joined the clade comprising the type strains of Oceanimonasspecies. Strain 102-Na3 exhibited 16S rRNA gene sequence similarity values of 98.8, 98.3 and 98.0 % to the type strains of Oceanimonas doudoroffii MBIC1298, Oceanimonas baumannii GB6 and Oceanimonas smirnovii 31-13, respectively. Strain 102-Na3 contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C12 : 0 as major fatty acids. The major quinone was ubiquinone-8. The polar lipids were composed of phosphatidylethanolamine,...

International journal of systematic and evolutionary microbiology, Jan 21, 2018

A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4, was isolate... more A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1-3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4 clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671 (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1 (96.4 %) and Zobellella taiwanensis ZT1 (96.0 %). The major fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C12 : 0, summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. Th...

The Journal of antibiotics, 2018

Hahella chejuensis MB-1084 is a Gram-negative marine bacterial strain that produces unusual 17-me... more Hahella chejuensis MB-1084 is a Gram-negative marine bacterial strain that produces unusual 17-membered carbocyclic tetraenes, chejuenolide A and B. Two fosmid clones responsible for chejuenolide production were identified from the genomic DNA library of the MB-1084 strain. Systematic inactivation of the open reading frames (ORFs) in the sequenced region defines the boundaries of the chejuenolide (che) biosynthetic gene cluster (24.9 kbp) that encodes one non-ribosomal peptide synthase (NRPS)-polyketide synthase (PKS) hybrid protein, three modular PKSs, two PKS domains, and an amine oxidase homolog. Based on the results, we found that the che PKSs have non-canonical features such as trans-AT system and insufficient number of KS domains (five KS domains) for chejuenolide production (requires eight rounds of Claisen condensation reaction). Heterologous expression of the che PKSs in the E. coli BAP1 strain provides strong evidence of the iterative characteristic of the modular PKSs. Ad...

Environmental pollution (Barking, Essex : 1987), Jan 4, 2017

The tidal flats near Sinduri beach in Taean, Korea, have been severely contaminated by heavy crud... more The tidal flats near Sinduri beach in Taean, Korea, have been severely contaminated by heavy crude oils due to the Korea's worst oil spill accident, say the Hebei Spirit Oil Spill, in 2007. Crude oil compounds, including polycyclic aromatic hydrocarbons (PAHs), pose significant environmental damages due to their wide distribution, persistence, high toxicity, mutagenicity, and carcinogenicity. Microbial community of Sinduri beach sediments samples was analyzed by metagenomic data with 16S rRNA gene amplicons. Three phyla (Proteobacteria, Firmicutes, and Bacteroidetes) accounted for approximately ≥93.0% of the total phyla based on metagenomic analysis. Proteobacteria was the dominant phylum in Sinduri beach sediments. Cultivable bacteria were isolated from PAH-enriched cultures, and bacterial diversity was investigated through performing culture characterization followed by molecular biology methods. Sixty-seven isolates were obtained, comprising representatives of Actinobacteria,...

International journal of systematic and evolutionary microbiology, 2017

A novel Gram-stain-positive, irregularly rod-shaped actinomycete, designated strain 002-2T, was i... more A novel Gram-stain-positive, irregularly rod-shaped actinomycete, designated strain 002-2T, was isolated from sand beach sediment collected from the Taean seashore, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies of cells were bright yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid and two unknown amino phospholipids. The major fatty acids were iso-C16 : 0 and C17 : 1ω8c. The DNA G+C content was 68.8 mol%. 16S rRNA gene sequence analysis revealed that strain 002-2T belongs to the family Nocardioidaceae and formed a cluster with Nocardioides rubroscoriae Sco-A25T (98.1 % sequence similarity) and Nocardioides plantarum NCIMB 12834T (97.6 %). On the basis of the phenotypic and phylogenetic data, strain 002-2T (=KCTC 39838T=DSM 10...

Mycobiology, 2017

Despite the huge worldwide diversity of Trichoderma (Hypocreaceae, Ascomycota), only about 22 spe... more Despite the huge worldwide diversity of Trichoderma (Hypocreaceae, Ascomycota), only about 22 species have been reported in Korea. Thus, between 2013 and 2015, soil-derived Trichoderma spp. were isolated to reveal the diversity of Korean Trichoderma. Phylogenetic analysis of translation elongation factor 1 alpha gene was used for identification. Among the soil-derived Trichoderma, Trichoderma albolutescens, T. asperelloides, T. orientale, T. spirale, and T. tomentosum have not been previously reported in Korea. Thus, we report the five Trichoderma species as new in Korea with morphological descriptions and images.

International journal of systematic and evolutionary microbiology, Jan 17, 2016

The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain GSE06T, isolated from the ... more The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain GSE06T, isolated from the surface-sterilized root of a cucumber plant grown in a field in Gunsan, Korea, was characterized by not only cultural and morphological features but also physiological, biochemical, and molecular analyses. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSE06T was most related to species of the genus Chryseobacterium. Furthermore, strain GSE06T exhibited the highest sequence similarities with type strains of Chryseobacterium indologenes ATCC 29897T (98.9 %), Chryseobacterium gleum ATCC 35910T (98.8 %), Chryseobacterium arthrosphaerae CC-VM-7T (98.7 %), Chryseobacterium contaminans C26T (98.5 %), Chryseobacterium artocarpi UTM-3T (98.3 %), and Chryseobacterium gallinarum 100T (97.9 %). Average nucleotide identity values between genome sequences of strain GSE06T and the above-mentioned reference strains ranged from 81.2 to 86.9 %, which were lower than the thres...

Food chemistry, Jan 15, 2017

Two different doenjang manufacturing processes, the industrial process (IP) and the modified indu... more Two different doenjang manufacturing processes, the industrial process (IP) and the modified industrial process (mIP) with specific microbial assortments, were subjected to metabolite profiling using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). The multivariate analyses indicated that both primary and secondary metabolites exhibited distinct patterns according to the fermentation processes (IP and mIP). Microbial community analysis for doenjang using denaturing gradient gel electrophoresis (DGGE), exhibited that both bacteria and fungi contributed proportionally for each step in the process viz., soybean, steaming, drying, meju fermentation, cooling, brining, and aging. Further, correlation analysis indicated that Aspergillus population was linked to sugar metabolism, Bacillus spp. with that of fatty acids, whereas Tetragenococcus and Zygosaccharomyces were found associated with amino acids. These results sugges...

The plant pathology journal, 2015

Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a toleran... more Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a tolerance to hydrolysis by proteases, and a favorable degradability under field conditions, which make them an attractive proposition for use as agricultural fungicides. Antimicrobial cyclic peptides are classified according to the types of bonds within the ring structure; homodetic, heterodetic, and complex cyclic peptides, which in turn reflect diverse physicochemical features. Most antimicrobial cyclic peptides affect the integrity of the cell envelope. This is achieved through direct interaction with the cell membrane or disturbance of the cell wall and membrane component biosynthesis such as chitin, glucan, and sphingolipid. These are specific and selective targets providing reliable activity and safety for non-target organisms. Synthetic cyclic peptides produced through combinatorial chemistry offer an alternative approach to develop antimicrobials for agricultural uses. Those synthesized...

International Journal of Systematic and Evolutionary Microbiology, 2007

A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB11... more A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB118T, was isolated from fresh water collected from the Woopo wetland (Republic of Korea). Cells were rod-shaped and sometimes filamentous. The major fatty acids were iso-C15 : 02-OH and/or C16 : 1ω7c(45.6 %), C16 : 1ω5c(18.5 %), iso-C15 : 0(9.5 %) and C16 : 0(8.8 %). The predominant menaquinone and polar lipid were MK-7 and phosphatidylethanolamine, respectively. The DNA G+C content was 53.3 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB118Thad an evolutionary lineage within the radiation encompassing the members of the family ‘Flexibacteraceae’, its closest neighbour beingSpirosoma lingualeLMG 10896T(93.7 % gene sequence similarity). Data from this polyphasic study indicated that strain WPCB118Tcould not be assigned to any recognized species. Strain WPCB118Trepresents a novel species of the genusSpirosoma, for which the nameSpirosoma riguisp. nov. is pr...

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008

A Gram-positive, cream-coloured, non-motile, rod-shaped actinomycete, designated DLS-10 T , was i... more A Gram-positive, cream-coloured, non-motile, rod-shaped actinomycete, designated DLS-10 T , was isolated from a rock collected on the peak of Darangshi Oreum (a small mountain 300 m above sea level) in Jeju, Republic of Korea. The temperature and pH ranges for growth were 4-37 6C and pH 5.1-9.1. The organism grew at NaCl concentrations up to 3 % and had the following chemotaxonomic characteristics: meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, the polar lipids included diphosphatidylglycerol, phosphatidylmethylethanolamine and two unknown phospholipids, the major menaquinone was MK-8(H 4) and the major fatty acids were anteiso-C 15 : 0 , C 16 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0. The DNA G+C content was 74.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism was related to members of the family Nakamurellaceae in the suborder Frankineae. The most closely related described organisms were the type strains of Humicoccus flavidus (97.1 % sequence similarity) and Nakamurella multipartita (95.2 %). On the basis of the morphological, cultural, physiological, chemotaxonomic and phylogenetic evidence presented here, the organism represents a novel genus and species of the family Nakamurellaceae, for which the name Saxeibacter lacteus gen. nov., sp. nov. is proposed. The type strain of Saxeibacter lacteus is DLS-10 T (5KCTC 19285 T 5DSM 19367 T).

International Journal of Systematic and Evolutionary Microbiology, 2009

A novel Gram-stain-positive, coccoid actinobacterium, designated strain Sco-D01T, was isolated fr... more A novel Gram-stain-positive, coccoid actinobacterium, designated strain Sco-D01T, was isolated from volcanic ash collected from Oreum (a parasitic volcanic cone) on Jeju Island, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies were vivid yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated, 10-methyl and hydroxyl components. The DNA G+C content of strain Sco-D01T was 72.0 mol%. 16S rRNA gene sequence analysis revealed that strain Sco-D01T belonged to the family Nocardioidaceae and formed a distinct sublineage within the radiation of the genus Marmoricola. The level of DNA–DNA relatedness between strain Sco-D01T and its closest phy...

International Journal of Systematic and Evolutionary Microbiology, 2010

A novel actinomycete, designated strain Sco-A36T, was isolated from volcanic ash. Cells were aero... more A novel actinomycete, designated strain Sco-A36T, was isolated from volcanic ash. Cells were aerobic, Gram-stain-positive, oxidase-negative, catalase-positive, non-motile cocci; colonies were yellow-coloured, smooth, entire and convex. Phylogenetic analysis showed that the organism formed a distinct phyletic line within the radiation of the genus Marmoricola. Its closest phylogenetic neighbours were Marmoricola aurantiacus DSM 12652T (97.9 % 16S rRNA gene sequence similarity), Marmoricola scoriae Sco-D01T (97.9 %), Marmoricola aequoreus SST-45T (97.4 %) and Marmoricola bigeumensis MSL-05T (96.3 %). The diagnostic diamino acid in the cell walls was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids contained phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The predominant fatty acids were C16 : 0, C17 : 1ω8c, C18 : 1ω9c and C16 : 1ω7c and/or iso-C15 : 0 2-OH. The DNA G+C content of str...

International Journal of Systematic and Evolutionary Microbiology, 2010

A novel actinomycete, designated strain Sco-B14T, was isolated from volcanic ash collected near D... more A novel actinomycete, designated strain Sco-B14T, was isolated from volcanic ash collected near Darangshi Oreum (a parasitic or satellite volcano) in Jeju, Republic of Korea. The organism formed well-developed, branched substrate mycelium, on which short chains of non-motile spores were arranged singly or in clusters. Aerial mycelium was not produced. Globose bodies were observed. The reverse colour of colonies was light brown to brown. Diffusible pigments were produced on ISP medium 3 and oatmeal-nitrate agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Sco-B14T formed a lineage within the family Micromonosporaceae and was distinct from established genera. The 16S rRNA gene sequence similarity of strain Sco-B14T to members of related genera of the family was 95.0–95.7 % to type strains of Catellatospora species, 94.7 % to Hamadaea tsunoensis IMSNU 22005T, 94.7 % to Longispora albida K97-0003T and 94.0 % to Catelliglobosispora koreensis LM 042T. 3-Hydro...

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009

A novel, Gram-negative bacterium, designated CS5-B2 T , was isolated from soil that had been coll... more A novel, Gram-negative bacterium, designated CS5-B2 T , was isolated from soil that had been collected from a cliff on Mara Island, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the radiation of the genus Dyella. The closest relatives were the type strains of Dyella koreensis, Dyella ginsengisoli and Dyella japonica and 16S rRNA gene sequence similarities between strain CS5-B2 T and these strains were 99.0, 97.9 and 97.8 %, respectively. The sequence similarities between the novel isolate and other related taxa compared in the phylogenetic analysis were less than 96.7 %. The cells of strain CS5-B2 T were aerobic, oxidase-negative, catalase-positive, motile rods. The temperature range for growth was 20-37 6C, with optimal growth at 30-37 6C. Growth occurred at pH 5.1-9.1, with optimal growth at pH 6.1-9.1. NaCl tolerance for growth was from 1 to 2 % (w/v). Ubiquinone-8 was the predominant respiratory lipoquinone. The major fatty acids were iso-C 15 : 0 and iso-C 17 : 1 v9c. The G+C content of the DNA was 65.7-66.6 mol%. The level of DNA-DNA relatedness with D. koreensis KCTC 12359 T was 20.2 and 29.6 % in duplicate measurements. On the basis of phenotypic features, phylogenetic analysis and DNA-DNA relatedness, a novel species of the genus Dyella is proposed, with the name Dyella marensis sp. nov. The type strain is CS5-B2 T (5JCM 14959 T 5KCTC 22144 T).

International journal of systematic and evolutionary microbiology, Jan 31, 2017

A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isola... more A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2T belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were 'Echinicola shivajiensis' AK12T (98.1 % 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754T (96.4 %), Echinicola jeungdonensis HMD 3054T (96.0 %), Echinicola pacifica KMM 6172T (96.0 %) and Echinicola rosea JL 3085T (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω6c and summed feature 9 (comprising iso-C17 : 1...

PLOS ONE, 2019

Probiotics are dietary supplements containing viable, non-pathogenic microorganisms that interact... more Probiotics are dietary supplements containing viable, non-pathogenic microorganisms that interact with the gastrointestinal microflora and directly with the immune system. The possible health effects of probiotics include modulating the immune system and exerting antibacterial, anticancer, and anti-mutagenic effects. The purpose of this study was to isolate, identify, and characterize novel strains of probiotics from the faeces of Korean infants. Various assays were conducted to determine the physiological features of candidate probiotic isolates, including Gram staining, 16S rRNA gene sequencing, tolerance assays to stimulated gastric juice and bile salts, adherence ability assays, antibiotic susceptibility testing, and assays of immunomodulatory effects. Based on these morphological and biochemical characteristics, five potential probiotic isolates (Enterococcus faecalis BioE EF71, Lactobacillus fermentum BioE LF11, Lactobacillus plantarum BioE LPL59, Lactobacillus paracasei BioE LP08, and Streptococcus thermophilus BioE ST107) were selected. E. faecalis BioE EF71 and L. plantarum BioE LPL59 showed high tolerance to stimulated gastric juice and bile salts, and S. thermophilus BioE ST107 as well as these two strains exhibited stronger adherence ability than reference strain Lactobacillus rhamnosus GG. All five strains inhibited secretion of lipopolysaccharide-induced pro-inflammatory cytokines IL-6 and TNF-α in RAW264.7 macrophages in vitro. L. fermentum BioE LF11, L. plantarum BioE LPL59, and S. thermophilus BioE ST107 enhanced the production of anti-inflammatory cytokine IL-10. Overall, our findings demonstrate that the five novel strains have potential as safe probiotics and encouraged varying degrees of immunomodulatory effects.

International Journal of Systematic and Evolutionary Microbiology, 2018

A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8, was isolate... more A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-S2-8, was isolated from a sea-tidal flat sediment sample from Gopado, Republic of Korea. Cells were aerobic, catalase-negative, oxidase-positive, non-motile and cocci, occurring singly, in pairs or in tetrads, and often tending to form aggregates. The strain grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-11.0 (pH 7.0-9.0) and in the presence of 0-11 % (w/v) NaCl (0-3 %). Phylogenetic analyses based on 16S rRNA gene sequences represented that the isolate belongs to the genus Blastococcus. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. Whole-cell sugar analysis of strain GP-S2-8 revealed rhamnose, glucose and mannose as characteristic sugars. The predominant respiratory quinone was MK-9(H4) and the major fatty acids were iso-C16 : 0, iso-C16 : 1 H, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C14 : 0. The polar lipid profile included diphosphadidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, one unidentified glycophospholipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content was 74.2 mol%. DNA-DNA relatedness values between strain GP-S2-8 and type strains of the genus Blastococcus ranged from 14.6 to 48.6 %. On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Blastococcus, for which the name Blastococcuslitoris sp. nov. is proposed. The type strain is GP-S2-8 (=KCCM 43275=JCM 32354=DSM 106127=KCTC 49078).

International Journal of Systematic and Evolutionary Microbiology, 2018

A novel Gram-stain-negative, moderately halophilic and aerobic bacterium, designated strain SDRB-... more A novel Gram-stain-negative, moderately halophilic and aerobic bacterium, designated strain SDRB-Phe2, was isolated from coastal sediment of the yellow sea in Sindu-ri, Republic of Korea. Cells were oxidase-positive, catalase-positive, rod-shaped and surrounded by a capsule with gliding motility. Colonies were yellow-coloured, circular, pulvinate with entire margins. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SDRB-Phe2 formed a distinct lineage within the genus Maribacter of the family Flavobacteriaceae. Stain SDRB-Phe2 exhibited 16S rRNA gene sequence similarity values of 97.1-98.9 % to the type strains of Maribacterstanieri, Maribacterspongiicola, Maribacter forsetii, Maribacter dokdonensis, Maribacter aquivivus, Maribactercaenipelagi, Maribacterlitorisediminis, Maribactersedimenticola, Maribacterulvicola, Maribacter confluentis and Maribacter orientalis, and of 94.8-96.7 % to the type strains of the other species of the genus Maribacter. Strain SDRB-Phe2 contained MK-6 as the predominant respiratory quinone and iso-C15 : 1 G, iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The polar lipids of strain SDRB-Phe2 were phosphatidylethanolamine, one unidentified amino lipid and two unidentified lipids. The DNA G+C content was 36.2 mol%. DNA-DNA relatedness values of strain SDRB-Phe2 to the type strains of the 11 phylogenetically related species of the genus Maribacter were 21.9-38.6 %. On the basis of the phenotypic features, phylogenetic and DNA-DNA hybridization analyses presented here, strain SDRB-Phe2 (=JCM 32373=KCTC 62273=DSM 106042) represents a novel species of the genus Maribacter, for which the name Maribacterlitoralis sp. nov. is proposed.

Environmental pollution (Barking, Essex : 1987), 2018

Crude oil and its derivatives are considered as one group of the most pervasive environmental pol... more Crude oil and its derivatives are considered as one group of the most pervasive environmental pollutants in marine environments. Bioremediation using oil-degrading bacteria has emerged as a promising green cleanup alternative in more recent years. The employment of biosurfactant-producing and hydrocarbon-utilizing indigenous bacteria enhances the effectiveness of bioremediation by making hydrocarbons bioavailable for degradation. In this study, the best candidates of biosurfactant-producing indigenous bacteria were selected by screening of biochemical tests. The selected bacteria include Bacillus algicola (003-Phe1), Rhodococcus soli (102-Na5), Isoptericola chiayiensis (103-Na4), and Pseudoalteromonas agarivorans (SDRB-Py1). In general, these isolated species caused low surface tension values (33.9-41.3 mN m), high oil spreading (1.2-2.4 cm), and hydrocarbon emulsification (up to 65%) warranting active degradation of hydrocarbons. FT-IR and LC-MS analyses indicated that the monorham...

International journal of systematic and evolutionary microbiology, Jan 24, 2018

A Gram-stain-negative, strictly aerobic, motile and rod-shaped bacterial strain, designated 102-N... more A Gram-stain-negative, strictly aerobic, motile and rod-shaped bacterial strain, designated 102-Na3, was isolated from sediment of Sinduri beach in Taean, Republic of Korea. Strain 102-Na3 grew optimally at 28-37 °C, at pH 7.0-11.0 and in the presence of 1-3 % (w/v) NaCl, but NaCl was not an absolute requirement for growth. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain 102-Na3 joined the clade comprising the type strains of Oceanimonasspecies. Strain 102-Na3 exhibited 16S rRNA gene sequence similarity values of 98.8, 98.3 and 98.0 % to the type strains of Oceanimonas doudoroffii MBIC1298, Oceanimonas baumannii GB6 and Oceanimonas smirnovii 31-13, respectively. Strain 102-Na3 contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C12 : 0 as major fatty acids. The major quinone was ubiquinone-8. The polar lipids were composed of phosphatidylethanolamine,...

International journal of systematic and evolutionary microbiology, Jan 21, 2018

A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4, was isolate... more A novel Gram-stain-negative, motile, rod-shaped bacterium, designated strain 102-Py4, was isolated from Sinduri beach sediment in Taean, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. The isolate grew optimally with 1-3 % (w/v) NaCl, but NaCl is not an absolute requirement for growth. 16S rRNA gene sequence analysis revealed that strain 102-Py4 clustered together with Zobellella aerophila and fell within the clade formed by recognized species of the genus Zobellella. Its closest phylogenetic neighbours were Z. aerophila JC2671 (98.1 % 16S rRNA gene sequence similarity), Zobellella denitrificans ZD1 (96.4 %) and Zobellella taiwanensis ZT1 (96.0 %). The major fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C12 : 0, summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and two unidentified amino lipids. The DNA G+C content was 62.1 mol%. Th...

The Journal of antibiotics, 2018

Hahella chejuensis MB-1084 is a Gram-negative marine bacterial strain that produces unusual 17-me... more Hahella chejuensis MB-1084 is a Gram-negative marine bacterial strain that produces unusual 17-membered carbocyclic tetraenes, chejuenolide A and B. Two fosmid clones responsible for chejuenolide production were identified from the genomic DNA library of the MB-1084 strain. Systematic inactivation of the open reading frames (ORFs) in the sequenced region defines the boundaries of the chejuenolide (che) biosynthetic gene cluster (24.9 kbp) that encodes one non-ribosomal peptide synthase (NRPS)-polyketide synthase (PKS) hybrid protein, three modular PKSs, two PKS domains, and an amine oxidase homolog. Based on the results, we found that the che PKSs have non-canonical features such as trans-AT system and insufficient number of KS domains (five KS domains) for chejuenolide production (requires eight rounds of Claisen condensation reaction). Heterologous expression of the che PKSs in the E. coli BAP1 strain provides strong evidence of the iterative characteristic of the modular PKSs. Ad...

Environmental pollution (Barking, Essex : 1987), Jan 4, 2017

The tidal flats near Sinduri beach in Taean, Korea, have been severely contaminated by heavy crud... more The tidal flats near Sinduri beach in Taean, Korea, have been severely contaminated by heavy crude oils due to the Korea's worst oil spill accident, say the Hebei Spirit Oil Spill, in 2007. Crude oil compounds, including polycyclic aromatic hydrocarbons (PAHs), pose significant environmental damages due to their wide distribution, persistence, high toxicity, mutagenicity, and carcinogenicity. Microbial community of Sinduri beach sediments samples was analyzed by metagenomic data with 16S rRNA gene amplicons. Three phyla (Proteobacteria, Firmicutes, and Bacteroidetes) accounted for approximately ≥93.0% of the total phyla based on metagenomic analysis. Proteobacteria was the dominant phylum in Sinduri beach sediments. Cultivable bacteria were isolated from PAH-enriched cultures, and bacterial diversity was investigated through performing culture characterization followed by molecular biology methods. Sixty-seven isolates were obtained, comprising representatives of Actinobacteria,...

International journal of systematic and evolutionary microbiology, 2017

A novel Gram-stain-positive, irregularly rod-shaped actinomycete, designated strain 002-2T, was i... more A novel Gram-stain-positive, irregularly rod-shaped actinomycete, designated strain 002-2T, was isolated from sand beach sediment collected from the Taean seashore, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies of cells were bright yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid and two unknown amino phospholipids. The major fatty acids were iso-C16 : 0 and C17 : 1ω8c. The DNA G+C content was 68.8 mol%. 16S rRNA gene sequence analysis revealed that strain 002-2T belongs to the family Nocardioidaceae and formed a cluster with Nocardioides rubroscoriae Sco-A25T (98.1 % sequence similarity) and Nocardioides plantarum NCIMB 12834T (97.6 %). On the basis of the phenotypic and phylogenetic data, strain 002-2T (=KCTC 39838T=DSM 10...

Mycobiology, 2017

Despite the huge worldwide diversity of Trichoderma (Hypocreaceae, Ascomycota), only about 22 spe... more Despite the huge worldwide diversity of Trichoderma (Hypocreaceae, Ascomycota), only about 22 species have been reported in Korea. Thus, between 2013 and 2015, soil-derived Trichoderma spp. were isolated to reveal the diversity of Korean Trichoderma. Phylogenetic analysis of translation elongation factor 1 alpha gene was used for identification. Among the soil-derived Trichoderma, Trichoderma albolutescens, T. asperelloides, T. orientale, T. spirale, and T. tomentosum have not been previously reported in Korea. Thus, we report the five Trichoderma species as new in Korea with morphological descriptions and images.

International journal of systematic and evolutionary microbiology, Jan 17, 2016

The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain GSE06T, isolated from the ... more The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain GSE06T, isolated from the surface-sterilized root of a cucumber plant grown in a field in Gunsan, Korea, was characterized by not only cultural and morphological features but also physiological, biochemical, and molecular analyses. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSE06T was most related to species of the genus Chryseobacterium. Furthermore, strain GSE06T exhibited the highest sequence similarities with type strains of Chryseobacterium indologenes ATCC 29897T (98.9 %), Chryseobacterium gleum ATCC 35910T (98.8 %), Chryseobacterium arthrosphaerae CC-VM-7T (98.7 %), Chryseobacterium contaminans C26T (98.5 %), Chryseobacterium artocarpi UTM-3T (98.3 %), and Chryseobacterium gallinarum 100T (97.9 %). Average nucleotide identity values between genome sequences of strain GSE06T and the above-mentioned reference strains ranged from 81.2 to 86.9 %, which were lower than the thres...

Food chemistry, Jan 15, 2017

Two different doenjang manufacturing processes, the industrial process (IP) and the modified indu... more Two different doenjang manufacturing processes, the industrial process (IP) and the modified industrial process (mIP) with specific microbial assortments, were subjected to metabolite profiling using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). The multivariate analyses indicated that both primary and secondary metabolites exhibited distinct patterns according to the fermentation processes (IP and mIP). Microbial community analysis for doenjang using denaturing gradient gel electrophoresis (DGGE), exhibited that both bacteria and fungi contributed proportionally for each step in the process viz., soybean, steaming, drying, meju fermentation, cooling, brining, and aging. Further, correlation analysis indicated that Aspergillus population was linked to sugar metabolism, Bacillus spp. with that of fatty acids, whereas Tetragenococcus and Zygosaccharomyces were found associated with amino acids. These results sugges...

The plant pathology journal, 2015

Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a toleran... more Antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a tolerance to hydrolysis by proteases, and a favorable degradability under field conditions, which make them an attractive proposition for use as agricultural fungicides. Antimicrobial cyclic peptides are classified according to the types of bonds within the ring structure; homodetic, heterodetic, and complex cyclic peptides, which in turn reflect diverse physicochemical features. Most antimicrobial cyclic peptides affect the integrity of the cell envelope. This is achieved through direct interaction with the cell membrane or disturbance of the cell wall and membrane component biosynthesis such as chitin, glucan, and sphingolipid. These are specific and selective targets providing reliable activity and safety for non-target organisms. Synthetic cyclic peptides produced through combinatorial chemistry offer an alternative approach to develop antimicrobials for agricultural uses. Those synthesized...

International Journal of Systematic and Evolutionary Microbiology, 2007

A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB11... more A Gram-negative, yellow-pigmented bacterium capable of gliding motility, designated strain WPCB118T, was isolated from fresh water collected from the Woopo wetland (Republic of Korea). Cells were rod-shaped and sometimes filamentous. The major fatty acids were iso-C15 : 02-OH and/or C16 : 1ω7c(45.6 %), C16 : 1ω5c(18.5 %), iso-C15 : 0(9.5 %) and C16 : 0(8.8 %). The predominant menaquinone and polar lipid were MK-7 and phosphatidylethanolamine, respectively. The DNA G+C content was 53.3 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB118Thad an evolutionary lineage within the radiation encompassing the members of the family ‘Flexibacteraceae’, its closest neighbour beingSpirosoma lingualeLMG 10896T(93.7 % gene sequence similarity). Data from this polyphasic study indicated that strain WPCB118Tcould not be assigned to any recognized species. Strain WPCB118Trepresents a novel species of the genusSpirosoma, for which the nameSpirosoma riguisp. nov. is pr...

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008

A Gram-positive, cream-coloured, non-motile, rod-shaped actinomycete, designated DLS-10 T , was i... more A Gram-positive, cream-coloured, non-motile, rod-shaped actinomycete, designated DLS-10 T , was isolated from a rock collected on the peak of Darangshi Oreum (a small mountain 300 m above sea level) in Jeju, Republic of Korea. The temperature and pH ranges for growth were 4-37 6C and pH 5.1-9.1. The organism grew at NaCl concentrations up to 3 % and had the following chemotaxonomic characteristics: meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, the polar lipids included diphosphatidylglycerol, phosphatidylmethylethanolamine and two unknown phospholipids, the major menaquinone was MK-8(H 4) and the major fatty acids were anteiso-C 15 : 0 , C 16 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0. The DNA G+C content was 74.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the organism was related to members of the family Nakamurellaceae in the suborder Frankineae. The most closely related described organisms were the type strains of Humicoccus flavidus (97.1 % sequence similarity) and Nakamurella multipartita (95.2 %). On the basis of the morphological, cultural, physiological, chemotaxonomic and phylogenetic evidence presented here, the organism represents a novel genus and species of the family Nakamurellaceae, for which the name Saxeibacter lacteus gen. nov., sp. nov. is proposed. The type strain of Saxeibacter lacteus is DLS-10 T (5KCTC 19285 T 5DSM 19367 T).

International Journal of Systematic and Evolutionary Microbiology, 2009

A novel Gram-stain-positive, coccoid actinobacterium, designated strain Sco-D01T, was isolated fr... more A novel Gram-stain-positive, coccoid actinobacterium, designated strain Sco-D01T, was isolated from volcanic ash collected from Oreum (a parasitic volcanic cone) on Jeju Island, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies were vivid yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated, 10-methyl and hydroxyl components. The DNA G+C content of strain Sco-D01T was 72.0 mol%. 16S rRNA gene sequence analysis revealed that strain Sco-D01T belonged to the family Nocardioidaceae and formed a distinct sublineage within the radiation of the genus Marmoricola. The level of DNA–DNA relatedness between strain Sco-D01T and its closest phy...

International Journal of Systematic and Evolutionary Microbiology, 2010

A novel actinomycete, designated strain Sco-A36T, was isolated from volcanic ash. Cells were aero... more A novel actinomycete, designated strain Sco-A36T, was isolated from volcanic ash. Cells were aerobic, Gram-stain-positive, oxidase-negative, catalase-positive, non-motile cocci; colonies were yellow-coloured, smooth, entire and convex. Phylogenetic analysis showed that the organism formed a distinct phyletic line within the radiation of the genus Marmoricola. Its closest phylogenetic neighbours were Marmoricola aurantiacus DSM 12652T (97.9 % 16S rRNA gene sequence similarity), Marmoricola scoriae Sco-D01T (97.9 %), Marmoricola aequoreus SST-45T (97.4 %) and Marmoricola bigeumensis MSL-05T (96.3 %). The diagnostic diamino acid in the cell walls was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids contained phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The predominant fatty acids were C16 : 0, C17 : 1ω8c, C18 : 1ω9c and C16 : 1ω7c and/or iso-C15 : 0 2-OH. The DNA G+C content of str...

International Journal of Systematic and Evolutionary Microbiology, 2010

A novel actinomycete, designated strain Sco-B14T, was isolated from volcanic ash collected near D... more A novel actinomycete, designated strain Sco-B14T, was isolated from volcanic ash collected near Darangshi Oreum (a parasitic or satellite volcano) in Jeju, Republic of Korea. The organism formed well-developed, branched substrate mycelium, on which short chains of non-motile spores were arranged singly or in clusters. Aerial mycelium was not produced. Globose bodies were observed. The reverse colour of colonies was light brown to brown. Diffusible pigments were produced on ISP medium 3 and oatmeal-nitrate agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Sco-B14T formed a lineage within the family Micromonosporaceae and was distinct from established genera. The 16S rRNA gene sequence similarity of strain Sco-B14T to members of related genera of the family was 95.0–95.7 % to type strains of Catellatospora species, 94.7 % to Hamadaea tsunoensis IMSNU 22005T, 94.7 % to Longispora albida K97-0003T and 94.0 % to Catelliglobosispora koreensis LM 042T. 3-Hydro...