Doreen Ciolek - Academia.edu (original) (raw)
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St Francis Xavier University (Nova Scotia, Canada)
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Papers by Doreen Ciolek
Analytical Biochemistry, 1997
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Journal of Protein Chemistry, 1994
During purification of recombinant Interleukin-2 (rIL-2) by reversed-phase HPLC, early fractions ... more During purification of recombinant Interleukin-2 (rIL-2) by reversed-phase HPLC, early fractions are discarded due to the presence of an unidentified form of rIL-2. A procedure has been developed to isolate and purify this unidentified form of rIL-2. The purification process involves two chromatography steps and utilizes a Bakerbond Carboxy-Sulfon (CS) column under two different conditions. This material, designated as a high-molecular-weight form of rIL-2 (HMWrIL-2), exhibits lower mobility during SDS-PAGE and has apI which is approximately one unit less than that of rIL-2, but has similar bioactivity to rIL-2. Structural analysis through enzymatic cleavage, HPLC peptide mapping, mass spectrometry, sequencing, and amino acid composition revealed that the difference between these two proteins is a C-terminal extension of 11 amino acids. This extension could be the result of a nonstandard translation event.
... Sequencing MeriLisa Bozzini, Jindong Zhao, Pau-Miau Yuan, Doreen Ciolek^, Yu-Ching Pan^, John... more ... Sequencing MeriLisa Bozzini, Jindong Zhao, Pau-Miau Yuan, Doreen Ciolek^, Yu-Ching Pan^, John Horton^, Daniel R. Marshak^, and Victoria L. Boyd. ... We also wish to thank Anne Marie Bozzini and Karen Felker for their editorial assistance and desktop publishing expertise. ...
Analytical Biochemistry, 1997
IngentaConnect. ...
Journal of Protein Chemistry, 1994
During purification of recombinant Interleukin-2 (rIL-2) by reversed-phase HPLC, early fractions ... more During purification of recombinant Interleukin-2 (rIL-2) by reversed-phase HPLC, early fractions are discarded due to the presence of an unidentified form of rIL-2. A procedure has been developed to isolate and purify this unidentified form of rIL-2. The purification process involves two chromatography steps and utilizes a Bakerbond Carboxy-Sulfon (CS) column under two different conditions. This material, designated as a high-molecular-weight form of rIL-2 (HMWrIL-2), exhibits lower mobility during SDS-PAGE and has apI which is approximately one unit less than that of rIL-2, but has similar bioactivity to rIL-2. Structural analysis through enzymatic cleavage, HPLC peptide mapping, mass spectrometry, sequencing, and amino acid composition revealed that the difference between these two proteins is a C-terminal extension of 11 amino acids. This extension could be the result of a nonstandard translation event.
... Sequencing MeriLisa Bozzini, Jindong Zhao, Pau-Miau Yuan, Doreen Ciolek^, Yu-Ching Pan^, John... more ... Sequencing MeriLisa Bozzini, Jindong Zhao, Pau-Miau Yuan, Doreen Ciolek^, Yu-Ching Pan^, John Horton^, Daniel R. Marshak^, and Victoria L. Boyd. ... We also wish to thank Anne Marie Bozzini and Karen Felker for their editorial assistance and desktop publishing expertise. ...