Dr Anita Tilwari - Academia.edu (original) (raw)
Papers by Dr Anita Tilwari
Current Journal of Applied Science and Technology
In the Rabi season of 2022, a comprehensive agricultural experiment was conducted at the Instruct... more In the Rabi season of 2022, a comprehensive agricultural experiment was conducted at the Instructional Farm of the Department of Agronomy, located within the Faculty of Agriculture at SAGE University in Bhopal, Madhya Pradesh. The primary objective of this experiment was to assess the influence of varying levels of phosphorus, in conjunction with the application of Phosphorus Solubilizing Bacteria (PSB), on the growth and yield parameters of field pea. This research was undertaken using a randomized block design, with a factorial arrangement that was replicated three times to ensure the reliability and robustness of the findings. The experiment encompassed seven distinct treatment combinations, which spanned the spectrum from a control group to different percentages of the recommended dose of phosphorus (RDF), including 100%, 80%, 60%, 40%, and 20% of the RDF. The results obtained from this meticulous study unveiled significant impacts of phosphorus levels and the application of Pho...
PubMed, Jun 1, 2011
Background: Various compounds of plant origin have been widely investigated since ancient times f... more Background: Various compounds of plant origin have been widely investigated since ancient times for their possible immunomodulatory properties as well as for the treatment of a wide range of diseases. Objective: To study the immunomodulatory functions of the aqueous extract of the seeds of Abrus precatorius commonly known as Indian liquorice (Fabaceae), a medicinal plant native to central India. Methods: Swiss albino mice were intraperitoneally treated with three doses (0.75, 1.25 and 2.5 µg/kg b.w.) of extract for 7 days. Relative organ weight, delayed type hypersensitivity (DTH) response, haemagglutination titre (HT) and Phagocytic index (PI) were studied in various groups of animals. Results: The results showed no significant difference in relative organ weight of spleen, liver, thymus and kidney in various groups of animals. Treatment of rats with increasing concentrations of the extract decreased the footpad thickness indicating a dose related inhibitory effect of the extract on delayed type hypersensitivity. In the HT test, the plant extract showed a suppressive effect at all doses, and these changes were significant as the dose increased. Phagocytic index was also increased in a dose dependent manner. Conclusion: The reduction of antibody titre, delayed type hypersensitivity response and the increase in phagocytic index indicates that Abrus precatorius has an inhibitory effect on the immune functions in mice.
Inorganic Chemistry Communications
Journal of Medicinal Plants Research, Jun 25, 2013
Rhizosphere biology, 2022
Journal of Ethnopharmacology, 1987
The interference of an aqueous extract of the stem bark of Azadirachta indica with different part... more The interference of an aqueous extract of the stem bark of Azadirachta indica with different parts of the human immune system was investigated. The extract showed strong anticomplementary effects which were dose-and time-dependent and most pronounced in the classical complement pathway assay. Moreover, a dose-dependent decrease in the chemiluminescence of polymorphonuclear leukocytes was observed and a dose-dependent increase in the production of migration inhibition factor by lymphocytes.
The Lancet Neurology, 2012
With thanks to our 2016 peer reviewers We are grateful to the following people for their signific... more With thanks to our 2016 peer reviewers We are grateful to the following people for their significant contribution to Health Promotion and Chronic Disease Prevention in Canada as peer reviewers in 2016. Their expertise ensures the quality of our journal and promotes the sharing of new knowledge among peers in Canada and internationally.
Frontiers in Plant Science
Non-rhizobial endophytes (NREs) are active colonizers inhabiting the root nodules. Though their a... more Non-rhizobial endophytes (NREs) are active colonizers inhabiting the root nodules. Though their active role in the lentil agroecosystem is not well defined, here we observed that these NREs might promote the growth of lentils, modulate rhizospheric community structure and could be used as promising organisms for optimal use of rice fallow soil. NREs from root nodules of lentils were isolated and examined for plant growth-promoting traits, exopolysaccharide (EPS) and biofilm production, root metabolites, and the presence of nifH and nifK elements. The greenhouse experiment with the chosen NREs, i.e., Serratia plymuthica 33GS and Serratia sp. R6 significantly increased the germination rate, vigour index, development of nodules (in non-sterile soil) and fresh weight of nodules (33GS 94%, R6 61% growth) and length of the shoot (33GS 86%, R6 51.16%) as well as chlorophyll levels when compared to the uninoculated control. Scanning Electron Microscopy (SEM) revealed that both isolates coul...
Journal of Inorganic and Organometallic Polymers and Materials
Design of antimicrobial tiles seems necessary to combat against contagious diseases, especially C... more Design of antimicrobial tiles seems necessary to combat against contagious diseases, especially COVID-19. In addition to personal hygiene, this technology facilitates public hygiene as antimicrobial tiles can be installed at hospitals, schools, banks, offices, lobbies, railway stations, etc. This review is primarily focused on preparing antimicrobial tiles using an antimicrobial layer or coatings that fight against germs. The salient features and working mechanisms of antimicrobial tiles are highlighted. This challenge is a component of the exploratory nature of nanoarchitectonics, that also extends farther than the realm of nanotechnology. This nanoarchitectonics has been successful at the laboratory scale as antimicrobial metal nanoparticles are mainly used as additives in preparing tiles. A detailed description of various materials for developing unique antimicrobial tiles is reported here. Pure metal (Ag, Zn) nanoparticles and a mixture of nanoparticles with other inorganic materials (SiO 2, , TiO 2 , anatase, nepheline) have been predominantly used to combat microbes. The developed antimicrobial tiles have shown excellent activity against a wide range of Gram-positive and Gram-negative bacteria. The last section discussed a hypothetical overview of utilizing the antimicrobial tiles against SARS-CoV-2. Overall, this review gives descriptive knowledge about the importance of antimicrobial tiles to create a clean and sustainable environment.
Advanced Nanocarbon Materials, 2022
Ashoka is the most ancient tree of India, generally known as a “ashok briksh”, botanically known ... more Ashoka is the most ancient tree of India, generally known as a “ashok briksh”, botanically known as a Saraca asoca (Roxb.). Saraca asoca has many uses mainly in the ayurvedic medicine. The endophytic fungus have developed a strong bonding with the plant during the co-evolution between them which resulted in production of bioactive Secondary metabolites which are originally the phytochemicals associated with the host plant. The aim of the present study was to isolate and observe antibacterial activity of the endophytic fungi against some pathogenic bacteria. Endophytic fungi (Syncephalastrum rasemosum, Syncephalastrum racemosums328) were isolated from mature healthy leaves and bark of medicinal plant Saraca asoca were collected from the Human Herbal Garden in M.P. Council of Science and Technology, Bhopal, Madhya Pradesh (India). Screening of endophytic fungi for in-vitro antibacterial activity against three pathogenic bacteria i.e. Streptococcus mutans, Lactobacillus acidophilus and...
Future Journal of Pharmaceutical Sciences, 2021
Background Fumaria parviflora (Lam.), commonly known as “fine-leaved fumitory,” is well known for... more Background Fumaria parviflora (Lam.), commonly known as “fine-leaved fumitory,” is well known for its therapeutic properties in the Indian traditional medicinal system. The presence of important bioactive compounds in plants makes them pharmacologically valuable. Therefore, in the present study, the high-performance thin layer chromatography (HPTLC) analysis of Fumaria parviflora (whole plant) methanolic extract was performed for its phytochemical profiling. Results The HPTLC densitometric analysis of the methanolic extract of Fumaria parviflora (whole plant) was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks. The phytochemical profile of the plant was determined and presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values. Conclusion The study concluded that F. parviflora methanolic extract of the whole ...
Molecular Biology Reports, 2021
Gloriosa superba L., an endangered medicinal plant with global interest due to presence of colchi... more Gloriosa superba L., an endangered medicinal plant with global interest due to presence of colchicine, an important alkaloid used in formulations of Indian and Traditional medicine. The plant has become endangered due to its unscientifically exploitation and high medicinal values. In the Present study 10 randomly amplified polymorphic DNA (RAPD) and 6 ISSR markers were employed to assess genetic divergence among micro propagated, wild and field cultivated plants of Gloriosa superba collected from different parts of India. In RAPD analysis, all the 10 accession with 10 RAPD primers amplified 466 fragments, with 96.43 % polymorphism and with an average of 46.6 bands per primer. The size of amplicons varied from 1656 to 100 bp. While, ISSR primers produced 328 fragments of which 298 were polymorphic with an average of 49.7 bands per primer with 91.83% polymorphism. The size of amplicons ranges from 2395 to 181 bp. RAPD, ISSR markers were also assessed by calculating polymorphic information content (PIC) to discriminate the genotypes, Average PIC value for RAPD, ISSR and combined RAPD + ISSR markers obtained was ≤ 0.50 suggesting the informativeness of markers. Jaccard's coefficient ranges from 0.18 to 0.75 (RAPD) and 0.17 to 0.61 (ISSR) and 0.21-0.52 for pooled ISSR and RAPD markers. The clustering pattern based on UPGMA analysis of the genotypes in the combined analysis revealed that the majority of the genotypes remained similar to the ISSR dendrogram, while the RAPD-based dendrogram showed some variation in the clustering of genotypes. The result of PCA scattered plot obtained were in agreement with the UPGMA dendrogram, which further confirms the genetic relationships explain by cluster analysis. Results confirmed that the genotype studied had good genetic diversity and can be used for identification, conservation, and future breeding program of Gloriosa species and consequently for the benefit of the pharmaceutical industries.
Frontiers in Genetics, 2021
Hot springs are considered to be a unique environment with extremophiles, that are sources of ind... more Hot springs are considered to be a unique environment with extremophiles, that are sources of industrially important enzymes, and other biotechnological products. The objective of this study was to undertake, analyze, and characterize the microbiome of two major hot springs located in the state of Madhya Pradesh explicitly, Chhoti Anhoni (Hotspring 1), and Badi Anhoni (Hotspring 2) to find out the inhabitant microbial population, and their functional characteristics. The taxonomic analysis of the microbiome of the hot springs revealed the phylum Proteobacteria was the most abundant taxa in both the hot-springs, however, its abundance in hot-spring 1 (~88%) was more than the hot-spring 2 (~52%). The phylum Bacteroides (~10–22%) was found to be the second most abundant group in the hot-springs followed by Spirocheates (~2–11%), Firmicutes (~6–8%), Chloroflexi (1–5%), etc. The functional analysis of the microbiome revealed different features related to several functions including metab...
Journal of Sol-Gel Science and Technology, 2020
The main objective of this paper is to synthesize advanced hybrid titanium compounds/F-MWCNTs nan... more The main objective of this paper is to synthesize advanced hybrid titanium compounds/F-MWCNTs nanocomposites for biomedical applications using a low temperature chemical method followed by an annealing processing. The uniqueness of our approach consists of utilizing the raw materials namely titanium compounds with varying morphological shapes i.e., spherical and nanoflowers nanoparticles for developing advanced nanocomposites A & B having hybrid organic-inorganic titanium compounds namely titanium oxide and sodium titanium oxide, which are conformally impregnated on F-MWCNTs and are useful for biomedical applications. Various techniques such as X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), ThermoGravimetric Analysis (TGA), X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Transmission Scanning Electron Microscopy (TEM), and Energy Dispersive X-ray Analysis (EDX), were performed to study and confirm the structures of the developed advanced hybrid titanium compounds F-MWCNTS nanocomposite. The developed nanocomposite A has shown antibacterial potential against test microbes, gram positive, and gram negative bacteria namely Staphylococcus aureus, Escheria coli, Lactobacillus planetarum, Lactobacillus acidophilus, and Enterococcus faecalis using the disc diffusion method at different concentrations. The developed material is useful for various applications like antibacterial agents, sodium insertion material of sodium ion batteries, shielding radiations like UV, X-rays while performing dental radiographic examinations of the patients.
Medicinal & Aromatic Plants, 2016
The RAPD markers are an efficient tool for assessing the genetic diversity and are less expensive... more The RAPD markers are an efficient tool for assessing the genetic diversity and are less expensive then molecular techniques like AFLP, RFLPs etc. therefore more convenient for use in research.This book basically covers the basic methodology used for assessing the genetic diversity like DNA isolation,quantitative and qualitative Estimation of DNA,RAPD-PCR,Agarose gel electrophoresis, Gel documentation,and use of Bioinformatics tool for data analysis. Rapid molecular testing techniques for the verification of elite genotypes/cultivars identity assume importance in the face of demands for exacting quality in international trade, need for ensuring homogeneity in planting stocks and protection of intellectual property rights on indigenous biodiversity of O. sanctum present in central India. The present study has shown the usefulness of RAPD markers for diversity analysis of O sanctum genotypes as well as cultivars obtained from different localities, particularly identification of the spe...
Current Journal of Applied Science and Technology
In the Rabi season of 2022, a comprehensive agricultural experiment was conducted at the Instruct... more In the Rabi season of 2022, a comprehensive agricultural experiment was conducted at the Instructional Farm of the Department of Agronomy, located within the Faculty of Agriculture at SAGE University in Bhopal, Madhya Pradesh. The primary objective of this experiment was to assess the influence of varying levels of phosphorus, in conjunction with the application of Phosphorus Solubilizing Bacteria (PSB), on the growth and yield parameters of field pea. This research was undertaken using a randomized block design, with a factorial arrangement that was replicated three times to ensure the reliability and robustness of the findings. The experiment encompassed seven distinct treatment combinations, which spanned the spectrum from a control group to different percentages of the recommended dose of phosphorus (RDF), including 100%, 80%, 60%, 40%, and 20% of the RDF. The results obtained from this meticulous study unveiled significant impacts of phosphorus levels and the application of Pho...
PubMed, Jun 1, 2011
Background: Various compounds of plant origin have been widely investigated since ancient times f... more Background: Various compounds of plant origin have been widely investigated since ancient times for their possible immunomodulatory properties as well as for the treatment of a wide range of diseases. Objective: To study the immunomodulatory functions of the aqueous extract of the seeds of Abrus precatorius commonly known as Indian liquorice (Fabaceae), a medicinal plant native to central India. Methods: Swiss albino mice were intraperitoneally treated with three doses (0.75, 1.25 and 2.5 µg/kg b.w.) of extract for 7 days. Relative organ weight, delayed type hypersensitivity (DTH) response, haemagglutination titre (HT) and Phagocytic index (PI) were studied in various groups of animals. Results: The results showed no significant difference in relative organ weight of spleen, liver, thymus and kidney in various groups of animals. Treatment of rats with increasing concentrations of the extract decreased the footpad thickness indicating a dose related inhibitory effect of the extract on delayed type hypersensitivity. In the HT test, the plant extract showed a suppressive effect at all doses, and these changes were significant as the dose increased. Phagocytic index was also increased in a dose dependent manner. Conclusion: The reduction of antibody titre, delayed type hypersensitivity response and the increase in phagocytic index indicates that Abrus precatorius has an inhibitory effect on the immune functions in mice.
Inorganic Chemistry Communications
Journal of Medicinal Plants Research, Jun 25, 2013
Rhizosphere biology, 2022
Journal of Ethnopharmacology, 1987
The interference of an aqueous extract of the stem bark of Azadirachta indica with different part... more The interference of an aqueous extract of the stem bark of Azadirachta indica with different parts of the human immune system was investigated. The extract showed strong anticomplementary effects which were dose-and time-dependent and most pronounced in the classical complement pathway assay. Moreover, a dose-dependent decrease in the chemiluminescence of polymorphonuclear leukocytes was observed and a dose-dependent increase in the production of migration inhibition factor by lymphocytes.
The Lancet Neurology, 2012
With thanks to our 2016 peer reviewers We are grateful to the following people for their signific... more With thanks to our 2016 peer reviewers We are grateful to the following people for their significant contribution to Health Promotion and Chronic Disease Prevention in Canada as peer reviewers in 2016. Their expertise ensures the quality of our journal and promotes the sharing of new knowledge among peers in Canada and internationally.
Frontiers in Plant Science
Non-rhizobial endophytes (NREs) are active colonizers inhabiting the root nodules. Though their a... more Non-rhizobial endophytes (NREs) are active colonizers inhabiting the root nodules. Though their active role in the lentil agroecosystem is not well defined, here we observed that these NREs might promote the growth of lentils, modulate rhizospheric community structure and could be used as promising organisms for optimal use of rice fallow soil. NREs from root nodules of lentils were isolated and examined for plant growth-promoting traits, exopolysaccharide (EPS) and biofilm production, root metabolites, and the presence of nifH and nifK elements. The greenhouse experiment with the chosen NREs, i.e., Serratia plymuthica 33GS and Serratia sp. R6 significantly increased the germination rate, vigour index, development of nodules (in non-sterile soil) and fresh weight of nodules (33GS 94%, R6 61% growth) and length of the shoot (33GS 86%, R6 51.16%) as well as chlorophyll levels when compared to the uninoculated control. Scanning Electron Microscopy (SEM) revealed that both isolates coul...
Journal of Inorganic and Organometallic Polymers and Materials
Design of antimicrobial tiles seems necessary to combat against contagious diseases, especially C... more Design of antimicrobial tiles seems necessary to combat against contagious diseases, especially COVID-19. In addition to personal hygiene, this technology facilitates public hygiene as antimicrobial tiles can be installed at hospitals, schools, banks, offices, lobbies, railway stations, etc. This review is primarily focused on preparing antimicrobial tiles using an antimicrobial layer or coatings that fight against germs. The salient features and working mechanisms of antimicrobial tiles are highlighted. This challenge is a component of the exploratory nature of nanoarchitectonics, that also extends farther than the realm of nanotechnology. This nanoarchitectonics has been successful at the laboratory scale as antimicrobial metal nanoparticles are mainly used as additives in preparing tiles. A detailed description of various materials for developing unique antimicrobial tiles is reported here. Pure metal (Ag, Zn) nanoparticles and a mixture of nanoparticles with other inorganic materials (SiO 2, , TiO 2 , anatase, nepheline) have been predominantly used to combat microbes. The developed antimicrobial tiles have shown excellent activity against a wide range of Gram-positive and Gram-negative bacteria. The last section discussed a hypothetical overview of utilizing the antimicrobial tiles against SARS-CoV-2. Overall, this review gives descriptive knowledge about the importance of antimicrobial tiles to create a clean and sustainable environment.
Advanced Nanocarbon Materials, 2022
Ashoka is the most ancient tree of India, generally known as a “ashok briksh”, botanically known ... more Ashoka is the most ancient tree of India, generally known as a “ashok briksh”, botanically known as a Saraca asoca (Roxb.). Saraca asoca has many uses mainly in the ayurvedic medicine. The endophytic fungus have developed a strong bonding with the plant during the co-evolution between them which resulted in production of bioactive Secondary metabolites which are originally the phytochemicals associated with the host plant. The aim of the present study was to isolate and observe antibacterial activity of the endophytic fungi against some pathogenic bacteria. Endophytic fungi (Syncephalastrum rasemosum, Syncephalastrum racemosums328) were isolated from mature healthy leaves and bark of medicinal plant Saraca asoca were collected from the Human Herbal Garden in M.P. Council of Science and Technology, Bhopal, Madhya Pradesh (India). Screening of endophytic fungi for in-vitro antibacterial activity against three pathogenic bacteria i.e. Streptococcus mutans, Lactobacillus acidophilus and...
Future Journal of Pharmaceutical Sciences, 2021
Background Fumaria parviflora (Lam.), commonly known as “fine-leaved fumitory,” is well known for... more Background Fumaria parviflora (Lam.), commonly known as “fine-leaved fumitory,” is well known for its therapeutic properties in the Indian traditional medicinal system. The presence of important bioactive compounds in plants makes them pharmacologically valuable. Therefore, in the present study, the high-performance thin layer chromatography (HPTLC) analysis of Fumaria parviflora (whole plant) methanolic extract was performed for its phytochemical profiling. Results The HPTLC densitometric analysis of the methanolic extract of Fumaria parviflora (whole plant) was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks. The phytochemical profile of the plant was determined and presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values. Conclusion The study concluded that F. parviflora methanolic extract of the whole ...
Molecular Biology Reports, 2021
Gloriosa superba L., an endangered medicinal plant with global interest due to presence of colchi... more Gloriosa superba L., an endangered medicinal plant with global interest due to presence of colchicine, an important alkaloid used in formulations of Indian and Traditional medicine. The plant has become endangered due to its unscientifically exploitation and high medicinal values. In the Present study 10 randomly amplified polymorphic DNA (RAPD) and 6 ISSR markers were employed to assess genetic divergence among micro propagated, wild and field cultivated plants of Gloriosa superba collected from different parts of India. In RAPD analysis, all the 10 accession with 10 RAPD primers amplified 466 fragments, with 96.43 % polymorphism and with an average of 46.6 bands per primer. The size of amplicons varied from 1656 to 100 bp. While, ISSR primers produced 328 fragments of which 298 were polymorphic with an average of 49.7 bands per primer with 91.83% polymorphism. The size of amplicons ranges from 2395 to 181 bp. RAPD, ISSR markers were also assessed by calculating polymorphic information content (PIC) to discriminate the genotypes, Average PIC value for RAPD, ISSR and combined RAPD + ISSR markers obtained was ≤ 0.50 suggesting the informativeness of markers. Jaccard's coefficient ranges from 0.18 to 0.75 (RAPD) and 0.17 to 0.61 (ISSR) and 0.21-0.52 for pooled ISSR and RAPD markers. The clustering pattern based on UPGMA analysis of the genotypes in the combined analysis revealed that the majority of the genotypes remained similar to the ISSR dendrogram, while the RAPD-based dendrogram showed some variation in the clustering of genotypes. The result of PCA scattered plot obtained were in agreement with the UPGMA dendrogram, which further confirms the genetic relationships explain by cluster analysis. Results confirmed that the genotype studied had good genetic diversity and can be used for identification, conservation, and future breeding program of Gloriosa species and consequently for the benefit of the pharmaceutical industries.
Frontiers in Genetics, 2021
Hot springs are considered to be a unique environment with extremophiles, that are sources of ind... more Hot springs are considered to be a unique environment with extremophiles, that are sources of industrially important enzymes, and other biotechnological products. The objective of this study was to undertake, analyze, and characterize the microbiome of two major hot springs located in the state of Madhya Pradesh explicitly, Chhoti Anhoni (Hotspring 1), and Badi Anhoni (Hotspring 2) to find out the inhabitant microbial population, and their functional characteristics. The taxonomic analysis of the microbiome of the hot springs revealed the phylum Proteobacteria was the most abundant taxa in both the hot-springs, however, its abundance in hot-spring 1 (~88%) was more than the hot-spring 2 (~52%). The phylum Bacteroides (~10–22%) was found to be the second most abundant group in the hot-springs followed by Spirocheates (~2–11%), Firmicutes (~6–8%), Chloroflexi (1–5%), etc. The functional analysis of the microbiome revealed different features related to several functions including metab...
Journal of Sol-Gel Science and Technology, 2020
The main objective of this paper is to synthesize advanced hybrid titanium compounds/F-MWCNTs nan... more The main objective of this paper is to synthesize advanced hybrid titanium compounds/F-MWCNTs nanocomposites for biomedical applications using a low temperature chemical method followed by an annealing processing. The uniqueness of our approach consists of utilizing the raw materials namely titanium compounds with varying morphological shapes i.e., spherical and nanoflowers nanoparticles for developing advanced nanocomposites A & B having hybrid organic-inorganic titanium compounds namely titanium oxide and sodium titanium oxide, which are conformally impregnated on F-MWCNTs and are useful for biomedical applications. Various techniques such as X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), ThermoGravimetric Analysis (TGA), X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Transmission Scanning Electron Microscopy (TEM), and Energy Dispersive X-ray Analysis (EDX), were performed to study and confirm the structures of the developed advanced hybrid titanium compounds F-MWCNTS nanocomposite. The developed nanocomposite A has shown antibacterial potential against test microbes, gram positive, and gram negative bacteria namely Staphylococcus aureus, Escheria coli, Lactobacillus planetarum, Lactobacillus acidophilus, and Enterococcus faecalis using the disc diffusion method at different concentrations. The developed material is useful for various applications like antibacterial agents, sodium insertion material of sodium ion batteries, shielding radiations like UV, X-rays while performing dental radiographic examinations of the patients.
Medicinal & Aromatic Plants, 2016
The RAPD markers are an efficient tool for assessing the genetic diversity and are less expensive... more The RAPD markers are an efficient tool for assessing the genetic diversity and are less expensive then molecular techniques like AFLP, RFLPs etc. therefore more convenient for use in research.This book basically covers the basic methodology used for assessing the genetic diversity like DNA isolation,quantitative and qualitative Estimation of DNA,RAPD-PCR,Agarose gel electrophoresis, Gel documentation,and use of Bioinformatics tool for data analysis. Rapid molecular testing techniques for the verification of elite genotypes/cultivars identity assume importance in the face of demands for exacting quality in international trade, need for ensuring homogeneity in planting stocks and protection of intellectual property rights on indigenous biodiversity of O. sanctum present in central India. The present study has shown the usefulness of RAPD markers for diversity analysis of O sanctum genotypes as well as cultivars obtained from different localities, particularly identification of the spe...