Dr. Arif Hasan Khan Robin (original) (raw)

Papers by Dr. Arif Hasan Khan Robin

Table S1. Probe numbers, identified SNP positions (red colour), probe sequences, and forward and ... more Table S1. Probe numbers, identified SNP positions (red colour), probe sequences, and forward and reverse primer sequences for the validation of ddRAD-seq-identified SNPs through high resolution melting. (DOCX 33 kb)

Plant Cell Reports, 2021

KEY MESSAGE 5-aminolevulinic acid (ALA) modulates various defense systems in plants and confers a... more KEY MESSAGE 5-aminolevulinic acid (ALA) modulates various defense systems in plants and confers abiotic stress tolerance. Enhancement of crop production is a challenge due to numerous abiotic stresses such as, salinity, drought, temperature, heavy metals, and UV. Plants often face one or more abiotic stresses in their life cycle because of the challenging growing environment which results in reduction of growth and yield. Diverse studies have been conducted to discern suitable mitigation strategies to enhance crop production by minimizing abiotic stress. Exogenous application of different plant growth regulators is a well-renowned approach to ameliorate adverse effects of abiotic stresses on crop plants. Among the numerous plant growth regulators, 5-aminolevulinic acid (ALA) is a novel plant growth regulator, also well-known to alleviate the injurious effects of abiotic stresses in plants. ALA enhances abiotic stress tolerance as well as growth and yield by regulating photosynthetic and antioxidant machineries and nutrient uptake in plants. However, the regulatory roles of ALA in plants under different stresses have not been studied and assembled systematically. Also, ALA-mediated abiotic stress tolerance mechanisms have not been fully elucidated yet. Therefore, this review discusses the role of ALA in crop growth enhancement as well as its ameliorative role in abiotic stress mitigation and also discusses the ALA-mediated abiotic stress tolerance mechanisms and its limitation and future promises for sustainable crop production.

Plants

Grasses have a segmental morphology. Compared to leaf development, data on root development at th... more Grasses have a segmental morphology. Compared to leaf development, data on root development at the phytomer level are scarce. Leaf appearance interval was recorded over time to allow inference about the age of segmental sites that later form roots. Hydroponically grown Lolium perenne cv. Aberdart tillers were studied in both spring and autumn in increasing and decreasing day length conditions, respectively, and dissected to define the development status of roots of known age on successive phytomers basipetally on the tiller axis. Over a 90-day observation period spring and autumn tillers produced 10.4 and 18.1 root bearing phytomers (Pr), respectively. Four stages of root development were identified: (0) main axis elongation (~0–10 days), (1) primary branching (~10–18 days), (2) secondary branching (~18–25 days), and (3) tertiary and quaternary branching without further increase in root dry weight. The individual spring roots achieved significantly greater dry weight (35%) than autu...

Alternaria blight is one of the most deadly diseases of oilseed Brassica. This recalcitrant disea... more Alternaria blight is one of the most deadly diseases of oilseed Brassica. This recalcitrant disease causes up to 50% yield loss across the globe. The disease is mainly caused by Alternaria brassicae and Alternaria brassicicola. These pathogens lack sexual stages and survive as conidia or condiospores on the debris of previous crops and susceptible weeds. Developing resistant oilseed Brassica cultivars to this disease has become a prime concern for researchers over the years. In absence of resistant oilseed Brassica cultivar, identification and introgression of resistance related genes can be a potential source for Alternaria blight resistance. As resistance toward Alternaria blight is governed by polygenes, intercrossing between the tolerant genotypes and subsequent selection will be the most appropriate way to transfer the quantitative resistance. For that reason, future breeding goal should focus on screening of germplasms for selecting genotypes containing resistance genes and st...

Salinity screening for 27 rice genotypes was performed at the seedling and reproductive stages re... more Salinity screening for 27 rice genotypes was performed at the seedling and reproductive stages respectively, in the hydroponic system and in sustained water bath. Three selected SSR markers were used to determine salinity tolerance in rice genotypes. Phenotyping of the germplasm was done at EC 12dS/m and 6dS/m at seedling and reproductive stages, respectively. Based on modified standard evaluation score for visual salt injury at seedling stage, eight genotypes were salt tolerant, four were moderately tolerant and the rest fifteen were susceptible. At the reproductive stage, six genotypes were tolerant to EC 6dS/m whereas eleven of them were susceptible. SSR based marker identified seven genotypes as tolerant but ten of them were susceptible for all three markers compared to two checks. Six genotypes were tolerant in both phenotypic and SSR screening. The indentified salt tolerant genotypes can be potential germplam sources for future breeding program.

The obligate biotroph Plasmodiophora brassicae causes clubroot disease in oilseeds and vegetables... more The obligate biotroph Plasmodiophora brassicae causes clubroot disease in oilseeds and vegetables of the Brassicaceae family, and cytokinins play a vital role in clubroot formation. In this study, we examined the expression patterns of 17 cytokinin-related genes involved in the biosynthesis, signaling, and degradation in Chinese cabbage inoculated with the Korean pathotype group 4 isolate of P. brassicae, Seosan. This isolate produced the most severe clubroot symptoms in Chinese cabbage cultivar “Bullam-3-ho” compared to three other Korean geographical isolates investigated. BrIPT1, a cytokinin biosynthesis gene, was induced on Day 1 and Day 28 in infected root tissues and the upregulation of this biosynthetic gene coincided with the higher expression of the response regulators BrRR1, on both Days and BrRR6 on Day 1 and 3. BrRR3 and 4 genes were also induced during gall enlargement on Day 35 in leaf tissues. The BrRR4 gene, which positively interact with phytochrome B, was consisten...

Wheat is one of the major cereal crops in Bangladesh. Over the last two decades, wheat consumptio... more Wheat is one of the major cereal crops in Bangladesh. Over the last two decades, wheat consumption has passionately amplified in Bangladesh but its production has declined due to various stress environments. Recurrent drought event due to climate change that threatens the country’s food safety has become a serious concern. To safeguard the food security, adopting suitable breeding strategies can add momentum. Developing drought tolerant wheat varieties are the definitive means of protecting the crop against hostile effects of drought. Plant breeders are exploring various breeding strategies to breed for the varieties that can cope with water deficient conditions well. Besides, breeders are consistently looking for new prospects and strategies that can boost genetic gain in yield. To endorse drought tolerance in wheat, understanding the physiological and genetic adaptation mechanisms of wheat cultivars during drought stress would provide the estimated benchmarks to adjust for suitabl...

Horticulture, Environment, and Biotechnology

Blackleg disease, caused by Leptosphaeria maculans, greatly affects the production of cabbage (Br... more Blackleg disease, caused by Leptosphaeria maculans, greatly affects the production of cabbage (Brassica oleracea). However, definitive R-gene(s) are yet to be identified in this crop. In contrast, a number of R-loci have been identified in A- or B-genome crops. Identification of few resistant cabbage genotypes indicates the presence of R-genes in this C-genome crop. High ancestral synteny between Brassica genomes suggests that the collinear regions of known A- or B-genome R-loci may also contain functional R-genes in the C-genome. Strong resistance was observed in the cotyledons of cabbage inbred line SCNU-98 against two L. maculans isolates, 03–02 s and 00–100 s. We investigated the collinear region of the Brassica napus blackleg resistance locus LepR2’ in B. oleracea since both isolates of L. maculans contain corresponding avirulence genes. The locus was collinear to a 5.8 Mbp genomic segment of B. oleracea chromosome C09 containing 13 genes that have putative disease resistance-r...

The Plant Pathology Journal

Clubroot is one of the most economically important diseases of the Brassicaceae family. Clubroot ... more Clubroot is one of the most economically important diseases of the Brassicaceae family. Clubroot disease is caused by the obligate parasite Plasmodiophora brassicae, which is difficult to study because it is nonculturable in the laboratory and its races are genetically variable worldwide. In Korea, there are at least five races that belongs to four pathotype groups. A recent study conducted in Korea attempted to develop molecular markers based on ribosomal DNA polymorphism to detect P. brassicae isolates, but none of those markers was either race-specific or pathotype-specific. Our current study aimed to develop race-and isolate-specific markers by exploiting genomic sequence variations. A total of 119 markers were developed based on unique variation exists in genomic sequences of each of the races. Only 12 markers were able to detect P. brassicae strains of each isolate or race. Ycheon14 markers was specific to isolates of race 2, Yeoncheon and Hoengseong. Ycheon9 and Ycheon10 markers were specific to Yeoncheon isolate (race 2, pathotype 3), ZJ1-3, ZJ1-4 and ZJ1-5 markers were specific to Haenam2 (race 4) isolate, ZJ1-35, ZJ1-40, ZJ1-41 and ZJ1-49 markers were specific to Hoengseong isolate and ZJ1-56 and ZJ1-64 markers were specific to Pyeongchang isolate (race 4, pathotype 3). The PCR-based sequence characterized amplified region (SCAR) markers developed in this study are able to detect five Korean isolates of P. brassicae. These markers can be utilized in identifying four Korean P. brassicae isolates from different regions. Additional effort is required to develop race-and isolate-specific markers for the remaining Korean isolates.

Biology and Life Sciences Forum

Wheat is an important cereal crop that often suffers from osmotic stress under various growing co... more Wheat is an important cereal crop that often suffers from osmotic stress under various growing conditions. The objective of this study was to investigate the effects of PEG-induced osmotic stress at the phytomer level on root growth and root hair morphology of 22 hydroponically grown wheat varieties. Two treatments, 0% and 10% PEG, were imposed on 20-day old wheat seedlings for a duration of 15 days. PEG stress significantly reduced plant height, number of live leaves per tiller, chlorophyll content, shoot dry weights, number of root bearing phytomers and roots per tiller. By contrast, PEG stress significantly increased leaf injury scores, root dry weight, main axis length and diameter of developed roots, length and diameter and density of both first and second order lateral roots, and the density and length of root hairs. An increase in root dry weight in PEG stress tolerant wheat genotypes was achieved through an increase in the length and diameter of main axis and lateral roots.

BMC Genomics

Background CONSTANS-like (CO-like, COL) are putative zinc-finger transcription factors known to p... more Background CONSTANS-like (CO-like, COL) are putative zinc-finger transcription factors known to play vital role in various plant biological processes such as control of flowering time, regulation of plant growth and development and responses to stresses. However, no systematic analysis of COL family gene regarding the plant development and stress response has been previously performed in any solanaceous crop. In the present study, a comprehensive genome-wide analysis of COL family genes in petunia has been conducted to figure out their roles in development of organs and stress response. Results A total of 33 COL genes, 15 PaCOL genes in P. axillaris and 18 PiCOL genes in P. inflata, were identified in petunia. Subsequently, a genome-wide systematic analysis was performed in 15 PaCOL genes. Considering the domain composition and sequence similarity the 15 PaCOL and 18 PiCOL genes were phylogenetically classified into three groups those are conserved among the flowering plants. Moreov...

Journal of Phytology

Maize (Zea mays L.) is one of the most important cereal crops cultivated around the world. Waterl... more Maize (Zea mays L.) is one of the most important cereal crops cultivated around the world. Waterlogging stress is a major production constraint of maize production in rain-fed agricultural systems. The main objective of this experiment was to investigate the effect of continuous waterlogging on morphological and biochemical traits of maize genotypes at the vegetative stage. Ten maize genotypes were treated under no waterlogging (control) and continuous waterlogging of five centimeters depth for 10 days. The treatments were applied to the plants at their 45 days of age. Visual leaf injury scores from Leaf 4 (youngest leaf is the reference point) to Leaf 7 separated tolerant and susceptible genotypes. Waterlogging stress significantly reduced the total number of live leaves and chlorophyll content in leaf tissues in susceptible genotypes. The anatomical study revealed that tolerant maize genotypes produce a large number of aerenchyma cells under waterlogging stress compared to suscept...

In Vitro Cellular & Developmental Biology - Plant

Embryo rescue technique is widely utilized to save developing embryo from abortion in interspecif... more Embryo rescue technique is widely utilized to save developing embryo from abortion in interspecific hybridization of plants. In Brassica species, success of an embryo rescue depends on the age of embryo, developmental stage of embryo, and media composition. In this study, ten crosses were conducted between diploid oilseed species B. rapa × tetraploid B. napus and B. rapa × tetraploid B. juncea along with their reciprocal crosses. The immature embryos from parents and crosses were cultured at 14 d after pollination in MS and ½ MS medium without any growth regulators for direct embryogenesis. Embryos were found at different stages of development during isolation, from globular to cotyledonary, indicating that rate of embryo development depends on genotype. Embryos isolated at the cotyledonary stage showed the highest regeneration percentage (av. 83.29%) and required the lowest time to regenerate (5.8 d) compared with another type of embryo, torpedo stage, cultured. Embryo rescued in ½ MS media accounted for 10–51%, 4.0–61%, 6.0–21%, and 5.0–43% greater shoot length, root length, number of leaves, and days to flowering, respectively, in different cross combinations compared with full-strength MS media. The results indicated that the rate of regeneration from embryo is higher in ½ MS media compared with full-strength MS media. Percent regeneration from the cultured embryos differed significantly in different cross combinations. Days required for regeneration from embryos, length of siliqua, and the number of ovules per siliqua accounted for high heterosis and broad sense heritability. The findings of this experiment would be useful in developing cost-effective embryo rescue protocol and desired interspecific hybrids through embryo rescue in Brassica species.

Plant Breeding and Biotechnology

Wheat is globally an important cereal crop. Environmental stress, especially drought stress can p... more Wheat is globally an important cereal crop. Environmental stress, especially drought stress can play an important role in the reduction of plant growth, specifically during germination in arid and semi-arid regions. Polyethylene glycol (PEG) treated hydroponic conditions create negative osmotic potential which is compared with moisture deficit stress. The main objective of this study was to investigate the effects of PEG 6000 induced moderate osmotic stress on germination indices of 22 wheat varieties. In order to study the effects of osmotic stress on germination indices in wheat cultivars, an experiment was conducted, using a completely randomized design with three replications under two different levels of PEG-6000: 0% and 10%. PEG stress significantly reduced percent germination, shoot length and root length. PEG stress significantly increased root-shoot ratio and oven dry weight. Principal component analysis revealed response of traits of tolerant wheat varieties under osmotic stress. Correlation study revealed the significant relationships among germination indices. The variety BARI Gom-30 recorded comparatively higher root length (6 cm), shoot length (7.8 cm), root-shoot ratio (1.37) followed by the variety Sonalika whereas the variety Kalaysona recorded the lowest root length (2.7 cm) and shoot length (2.8 cm) under PEG stress. Evolved information of this research including selected traits such as germination percentage, root-shoot ratio and dry weight of seedlings could be exploited in wheat breeding program for drought and osmotic stress tolerance.