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Papers by Dr. Brajesh Barse

Research paper thumbnail of Electrochemical Sensors and Their Applications: A Review

Chemosensors

The world of sensors is diverse and is advancing at a rapid pace due to the fact of its high dema... more The world of sensors is diverse and is advancing at a rapid pace due to the fact of its high demand and constant technological improvements. Electrochemical sensors provide a low-cost and convenient solution for the detection of variable analytes and are widely utilized in agriculture, food, and oil industries as well as in environmental and biomedical applications. The popularity of electrochemical sensing stems from two main advantages: the variability of the reporting signals, such as the voltage, current, overall power output, or electrochemical impedance, and the low theoretical detection limits that originate from the differences in the Faradaic and nonFaradaic currents. This review article attempts to cover the latest advances and applications of electrochemical sensors in different industries. The role of nanomaterials in electrochemical sensor research and advancements is also examined. We believe the information presented here will encourage further efforts on the understa...

Research paper thumbnail of India—GMOs/Synthetic Biology Rules/Regulations and Biodiversity – A Legal Perspective from India

Research paper thumbnail of Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study

Bioprocess and Biosystems Engineering, 2005

Research paper thumbnail of Cyclodextrins: Emerging Applications

Chimica oggi, 2003

Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glu... more Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glucopyranose subunits They are produced from starch by enzymatic degradation. These macrocyclic carbohydrates with apolar internal cavities can form complexes and solubilize many ...

Research paper thumbnail of Anaerobic Digestion of Organic Waste: An Overview

Environmental …, 2005

CHAPTER 50 ANAEROBIC DIGESTION OF ORGANIC WASTE: AN OVERVIEW Brajesh Barse, Mani Shankar Bhattach... more CHAPTER 50 ANAEROBIC DIGESTION OF ORGANIC WASTE: AN OVERVIEW Brajesh Barse, Mani Shankar Bhattacharya, Praveen Kaul, Anirban Banerjee, and UC Banerjee* Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and ...

Research paper thumbnail of Biocatalysis: An Emerging Field In the Synthesis of Eenantio-Pure Compounds

… in biochemistry and …, 2005

Research paper thumbnail of Biopolymer: A Sustainable Material for Food and Medical Applications

Polymers, 2022

Biopolymers are a leading class of functional material suitable for high-value applications and a... more Biopolymers are a leading class of functional material suitable for high-value applications and are of great interest to researchers and professionals across various disciplines. Interdisciplinary research is important to understand the basic and applied aspects of biopolymers to address several complex problems associated with good health and well-being. To reduce the environmental impact and dependence on fossil fuels, a lot of effort has gone into replacing synthetic polymers with biodegradable materials, especially those derived from natural resources. In this regard, many types of natural or biopolymers have been developed to meet the needs of ever-expanding applications. These biopolymers are currently used in food applications and are expanding their use in the pharmaceutical and medical industries due to their unique properties. This review focuses on the various uses of biopolymers in the food and medical industry and provides a future outlook for the biopolymer industry.

Research paper thumbnail of Market Analysis of Biomass for Biofuels and Biomaterials

Biomass for Bioenergy and Biomaterials

Research paper thumbnail of Bwnpub

Research paper thumbnail of Enantioselective nitrilase from Pseudomonas putida: cloning, heterologous expression, and bioreactor studies

Molecular biotechnology, 2009

Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic a... more Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase gene from P. putida was cloned in pET 21b(+) and over-expressed as histidine-tagged protein in Escherichia coli. The histidine-tagged enzyme was purified from crude cell extracts of IPTG-induced cells of E. coli BL21 (DE3). Inducer replacement studies led to the identification of lactose as a suitable and cheap alternative to the costly IPTG. Effects of medium components, various physico-chemical, and process parameters (pH, temperature, aeration, and agitation) for the production of nitrilase by engineered E. coli were optimized and scaled up to a laboratory scale bioreactor (6.6 l). Finally, the recombinant E. coli whole-cells were utilized for the production of (R)-(-)...

Research paper thumbnail of Hofmeister Challenges: Ion Binding and Charge of the BSA Protein as Explicit Examples

Langmuir, 2012

Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic l... more Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic light scattering (ELS) are used to study specific-ion binding. The effect is appreciable at a physiological concentration of 0.1 M. We found that anions bind to the protein surface at an acidic pH, where the protein carries a positive charge (Z(p) > 0), according to a Hofmeister series (Cl(-) < Br(-) < NO(3)(-) < I(-) < SCN(-)), as well as at the isoionic point (Z(p) = 0). The results obtained require critical interpretation. The measurements performed depend on electrostatic theories that ignore the very specific effects they are supposed to reveal. Notwithstanding this difficulty, we can still infer that different 1:1 sodium salts affect the BSA surface charge/pH curve because anions bind to the BSA surface with an efficiency which follows a Hofmeister series.

Research paper thumbnail of Studies on the production of enantioselective nitrilase in a stirred tank bioreactor by Pseudomonas putida MTCC 5110

Bioresource Technology, 2008

Nitrilases constitute an important class of hydrolases, having numerous industrial applications. ... more Nitrilases constitute an important class of hydrolases, having numerous industrial applications. The present work aims to address the production of nitrile hydrolyzing enzymes from Pseudomonas putida MTCC 5110 in a 6l bioreactor. Effect of various physico-chemical conditions and process parameters like pH, temperature, aeration and agitation rates and inducer concentration was studied. Further, the enzyme activity was enhanced by adopting the inducer feeding strategy. Various biochemical engineering parameters pertaining to the cultivation of P. putida in different physico-chemical conditions were reported. Finally, segregation of growth phase from the enzyme production phase allowed significant reduction in total fermentation time.

Research paper thumbnail of Advances in Biochemistry and Biotechnology Volume 1 Pages 53-80 Edited by: Dr. Chiranjib Chakraborty Published by: DAYA PUBLISHING HOUSE

Advances in biochemistry and …, 2005

Research paper thumbnail of Measurements and Theoretical Interpretation of Points of Zero Charge/Potential of BSA Protein

Langmuir, Aug 23, 2011

The points of zero charge/potential of proteins depend not only on pH but also on how they are me... more The points of zero charge/potential of proteins depend not only on pH but also on how they are measured. They depend also on background salt solution type and concentration. The protein isoelectric point (IEP) is determined by electrokinetical measurements, whereas the isoionic point (IIP) is determined by potentiometric titrations. Here we use potentiometric titration and zeta potential (ζ) measurements at different NaCl concentrations to study systematically the effect of ionic strength on the IEP and IIP of bovine serum albumin (BSA ...

Research paper thumbnail of Cyclodextrins: Emerging Applications

Chimica oggi, 2003

Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glu... more Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glucopyranose subunits They are produced from starch by enzymatic degradation. These macrocyclic carbohydrates with apolar internal cavities can form complexes and solubilize many ...

Research paper thumbnail of Correction to: Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study

Bioprocess and biosystems engineering, Jun 24, 2018

In the original version of our paper entitled "Release of an enantioselective nitrilase from... more In the original version of our paper entitled "Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study" (2005) 27:415-424, some references to already published articles were inadvertently left out.

Research paper thumbnail of Ion Specific Surface Charge Density of SBA-15 Mesoporous Silica

Langmuir

Potentiometric titrations were used to estimate the surface charge density of SBA-15 mesoporous s... more Potentiometric titrations were used to estimate the surface charge density of SBA-15 mesoporous silica in different salt solutions. It was found that surface charge depends both on cation type, following a Hofmeister series (Cs(+) < Guanidinium(+) < K(+) < Na(+) < Li(+)), and on salt concentration (in the range 0.05-1 M). The surface charge series is reproduced by theoretical calculations performed using a modified Poisson-Boltzmann equation that includes ionic dispersion forces with ab initio ion polarizabilities and hydrated ions. The hydration model assigns an explicit hydration shell to kosmotropic (strong hydrated) ions only. The Hofmeister series appears to be due to the combination of ion-surface dispersion interactions and ion hydration.

Research paper thumbnail of Electrochemical Sensors and Their Applications: A Review

Chemosensors

The world of sensors is diverse and is advancing at a rapid pace due to the fact of its high dema... more The world of sensors is diverse and is advancing at a rapid pace due to the fact of its high demand and constant technological improvements. Electrochemical sensors provide a low-cost and convenient solution for the detection of variable analytes and are widely utilized in agriculture, food, and oil industries as well as in environmental and biomedical applications. The popularity of electrochemical sensing stems from two main advantages: the variability of the reporting signals, such as the voltage, current, overall power output, or electrochemical impedance, and the low theoretical detection limits that originate from the differences in the Faradaic and nonFaradaic currents. This review article attempts to cover the latest advances and applications of electrochemical sensors in different industries. The role of nanomaterials in electrochemical sensor research and advancements is also examined. We believe the information presented here will encourage further efforts on the understa...

Research paper thumbnail of India—GMOs/Synthetic Biology Rules/Regulations and Biodiversity – A Legal Perspective from India

Research paper thumbnail of Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study

Bioprocess and Biosystems Engineering, 2005

Research paper thumbnail of Cyclodextrins: Emerging Applications

Chimica oggi, 2003

Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glu... more Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glucopyranose subunits They are produced from starch by enzymatic degradation. These macrocyclic carbohydrates with apolar internal cavities can form complexes and solubilize many ...

Research paper thumbnail of Anaerobic Digestion of Organic Waste: An Overview

Environmental …, 2005

CHAPTER 50 ANAEROBIC DIGESTION OF ORGANIC WASTE: AN OVERVIEW Brajesh Barse, Mani Shankar Bhattach... more CHAPTER 50 ANAEROBIC DIGESTION OF ORGANIC WASTE: AN OVERVIEW Brajesh Barse, Mani Shankar Bhattacharya, Praveen Kaul, Anirban Banerjee, and UC Banerjee* Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and ...

Research paper thumbnail of Biocatalysis: An Emerging Field In the Synthesis of Eenantio-Pure Compounds

… in biochemistry and …, 2005

Research paper thumbnail of Biopolymer: A Sustainable Material for Food and Medical Applications

Polymers, 2022

Biopolymers are a leading class of functional material suitable for high-value applications and a... more Biopolymers are a leading class of functional material suitable for high-value applications and are of great interest to researchers and professionals across various disciplines. Interdisciplinary research is important to understand the basic and applied aspects of biopolymers to address several complex problems associated with good health and well-being. To reduce the environmental impact and dependence on fossil fuels, a lot of effort has gone into replacing synthetic polymers with biodegradable materials, especially those derived from natural resources. In this regard, many types of natural or biopolymers have been developed to meet the needs of ever-expanding applications. These biopolymers are currently used in food applications and are expanding their use in the pharmaceutical and medical industries due to their unique properties. This review focuses on the various uses of biopolymers in the food and medical industry and provides a future outlook for the biopolymer industry.

Research paper thumbnail of Market Analysis of Biomass for Biofuels and Biomaterials

Biomass for Bioenergy and Biomaterials

Research paper thumbnail of Bwnpub

Research paper thumbnail of Enantioselective nitrilase from Pseudomonas putida: cloning, heterologous expression, and bioreactor studies

Molecular biotechnology, 2009

Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic a... more Nitrilases have attracted tremendous attention for the preparation of optically pure carboxylic acids. This article aims to address the production and utilization of a highly enantioselective nitrilase from Pseudomonas putida MTCC 5110 for the hydrolysis of racemic mandelonitrile to (R)-mandelic acid. The nitrilase gene from P. putida was cloned in pET 21b(+) and over-expressed as histidine-tagged protein in Escherichia coli. The histidine-tagged enzyme was purified from crude cell extracts of IPTG-induced cells of E. coli BL21 (DE3). Inducer replacement studies led to the identification of lactose as a suitable and cheap alternative to the costly IPTG. Effects of medium components, various physico-chemical, and process parameters (pH, temperature, aeration, and agitation) for the production of nitrilase by engineered E. coli were optimized and scaled up to a laboratory scale bioreactor (6.6 l). Finally, the recombinant E. coli whole-cells were utilized for the production of (R)-(-)...

Research paper thumbnail of Hofmeister Challenges: Ion Binding and Charge of the BSA Protein as Explicit Examples

Langmuir, 2012

Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic l... more Experiments on bovine serum albumin (BSA) via potentiometric titration (PT) and electrophoretic light scattering (ELS) are used to study specific-ion binding. The effect is appreciable at a physiological concentration of 0.1 M. We found that anions bind to the protein surface at an acidic pH, where the protein carries a positive charge (Z(p) &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 0), according to a Hofmeister series (Cl(-) &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; Br(-) &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; NO(3)(-) &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; I(-) &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; SCN(-)), as well as at the isoionic point (Z(p) = 0). The results obtained require critical interpretation. The measurements performed depend on electrostatic theories that ignore the very specific effects they are supposed to reveal. Notwithstanding this difficulty, we can still infer that different 1:1 sodium salts affect the BSA surface charge/pH curve because anions bind to the BSA surface with an efficiency which follows a Hofmeister series.

Research paper thumbnail of Studies on the production of enantioselective nitrilase in a stirred tank bioreactor by Pseudomonas putida MTCC 5110

Bioresource Technology, 2008

Nitrilases constitute an important class of hydrolases, having numerous industrial applications. ... more Nitrilases constitute an important class of hydrolases, having numerous industrial applications. The present work aims to address the production of nitrile hydrolyzing enzymes from Pseudomonas putida MTCC 5110 in a 6l bioreactor. Effect of various physico-chemical conditions and process parameters like pH, temperature, aeration and agitation rates and inducer concentration was studied. Further, the enzyme activity was enhanced by adopting the inducer feeding strategy. Various biochemical engineering parameters pertaining to the cultivation of P. putida in different physico-chemical conditions were reported. Finally, segregation of growth phase from the enzyme production phase allowed significant reduction in total fermentation time.

Research paper thumbnail of Advances in Biochemistry and Biotechnology Volume 1 Pages 53-80 Edited by: Dr. Chiranjib Chakraborty Published by: DAYA PUBLISHING HOUSE

Advances in biochemistry and …, 2005

Research paper thumbnail of Measurements and Theoretical Interpretation of Points of Zero Charge/Potential of BSA Protein

Langmuir, Aug 23, 2011

The points of zero charge/potential of proteins depend not only on pH but also on how they are me... more The points of zero charge/potential of proteins depend not only on pH but also on how they are measured. They depend also on background salt solution type and concentration. The protein isoelectric point (IEP) is determined by electrokinetical measurements, whereas the isoionic point (IIP) is determined by potentiometric titrations. Here we use potentiometric titration and zeta potential (ζ) measurements at different NaCl concentrations to study systematically the effect of ionic strength on the IEP and IIP of bovine serum albumin (BSA ...

Research paper thumbnail of Cyclodextrins: Emerging Applications

Chimica oggi, 2003

Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glu... more Cyclodextrins (CDs) are a family of cyclic oligosaccharides that are composed of α-1,4-linked glucopyranose subunits They are produced from starch by enzymatic degradation. These macrocyclic carbohydrates with apolar internal cavities can form complexes and solubilize many ...

Research paper thumbnail of Correction to: Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study

Bioprocess and biosystems engineering, Jun 24, 2018

In the original version of our paper entitled "Release of an enantioselective nitrilase from... more In the original version of our paper entitled "Release of an enantioselective nitrilase from Alcaligenes faecalis MTCC 126: a comparative study" (2005) 27:415-424, some references to already published articles were inadvertently left out.

Research paper thumbnail of Ion Specific Surface Charge Density of SBA-15 Mesoporous Silica

Langmuir

Potentiometric titrations were used to estimate the surface charge density of SBA-15 mesoporous s... more Potentiometric titrations were used to estimate the surface charge density of SBA-15 mesoporous silica in different salt solutions. It was found that surface charge depends both on cation type, following a Hofmeister series (Cs(+) < Guanidinium(+) < K(+) < Na(+) < Li(+)), and on salt concentration (in the range 0.05-1 M). The surface charge series is reproduced by theoretical calculations performed using a modified Poisson-Boltzmann equation that includes ionic dispersion forces with ab initio ion polarizabilities and hydrated ions. The hydration model assigns an explicit hydration shell to kosmotropic (strong hydrated) ions only. The Hofmeister series appears to be due to the combination of ion-surface dispersion interactions and ion hydration.