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Papers by Dr Disha Anilkumar Patel

Indian Journal of Microbiology Research, Nov 15, 2017

Influenza is a widespread viral infection and a major cause of morbidity and mortality worldwide.... more Influenza is a widespread viral infection and a major cause of morbidity and mortality worldwide. The present study was performed to investigate the Influenza A H1N1 2009 pandemic (influenza A(H1N1)pdm09) virus outbreaks, its pattern and its clino-epidemiological correlation in and around the region of Gujarat, during the period of August 2009 to March 2016 with special reference to outbreak of 2015. Materials and Method: This is a retrospective study. A total of 17206 patients attending various hospitals in Gujarat were included from August 2009 to March 2016. Nasal swabs, nasopharyngeal swabs, or throat swabs were collected and tested by real time Reverse Transcriptase PCR (rRTPCR) for presence of influenza A(H1N1)pdm09 virus. Results: Out of 17206 samples, 6078 clinical samples were positive for swine flu and 2605 were positive for seasonal Influenza A flu. Over all people in the age group between 30 years to 60 years were highest affected and females were affected more than males. Case fatality rate of the patients admitted in our hospital in 2015 outbreak was 23.07% with highest mortality in pregnant women. Conclusions: Influenza A(H1N1)pdm09 is still active six years after 2009 pandemic and unable to replace seasonal influenza A virus unlike previous pandemic strains. Middle age patients and pregnant women were highly affected with high mortality in pregnant women.

IP international journal of medical microbiology and tropical diseases, Apr 15, 2023

Background and Aim: Dengue is the most rapidly spreading mosquito borne viral disease of mankind,... more Background and Aim: Dengue is the most rapidly spreading mosquito borne viral disease of mankind, with a 30 fold increase in global incidence over the last five decades. It is a major public health concern throughout the tropical and subtropical regions of the world. The present study was conducted with objective to study epidemiological and demographic characteristics of dengue infection during the year 2019 in Kutch region, Gujarat, India.

BJ Kines: National Journal of Basic & Applied Sciences, Dec 10, 2022

Background & Aims: Neonatal septicemia is a major cause of neonatal mortality in developi... more Background & Aims: Neonatal septicemia is a major cause of neonatal mortality in developing countries. Rapid treatment with antimicrobials is very crucial for essential outcome. Methicillin Resistance Staphylococcus (MRS) are important cause of nosocomial infection, which causes septicemia especially in neonates. They are frequently resistance to other antibiotics also. So, rapid, sensitive and specific procedures are required urgently to initiate timely treatment. This study was undertaken to perform comparative evaluation of conventional methods with rapid agglutination method in diagnosis of MRS and to estimate the prevalence of MRS among neonatal septicemia cases. Materials and Method: This study was done in one of the major tertiary care hospitals in Ahmedabad, Gujarat. All the blood samples collected from the patients with sign and symptoms of septicemia, from January 2012 to July 2012, were cultured and identified by standard microbiological method. MRS detection was done by conventional methods and latex agglutination method. Results: Staphylococcus coagulase negative constituted 61.96% and S.aureus 4.33% of total bacterial isolates. Methicillin resistance in S.aureusand in CONS, was 44.44% and 25.91% respectively. Prevalence of MRS among neonates was 32.72%. The sensitivity and specificity of oxacillin disk was 68% and 100% respectively for detection of MRS. Latex agglutination test method shows sensitivity and specificity of 100% for detection of MRS. Conclusion: Along with cefoxitin disc diffusion method, latex agglutination method is to be used for early diagnosis & treatment and forprevention of over use of vancomycinfor methicillin resistant staphylococci. Keywords:Septicemia, MRS, Blood culture, S.aureus, Cefoxitin, CoNS

Indian Journal of Medical Research

Background & objectives: An outbreak of respiratory illness of unknown aetiology was reported fro... more Background & objectives: An outbreak of respiratory illness of unknown aetiology was reported from Hubei province of Wuhan, People's Republic of China, in December 2019. The outbreak was attributed to a novel coronavirus (CoV), named as severe acute respiratory syndrome (SARS)-CoV-2 and the disease as COVID-19. Within one month, cases were reported from 25 countries. In view of the novel viral strain with reported high morbidity, establishing early countrywide diagnosis to detect imported cases became critical. Here we describe the role of a countrywide network of VRDLs in early diagnosis of COVID-19. Methods: The Indian Council of Medical Research (ICMR)-National Institute of Virology (NIV), Pune, established screening as well as confirmatory assays for SARS-CoV-2. A total of 13 VRDLs were provided with the E gene screening real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay. VRDLs were selected on the basis of their presence near an international airport/seaport

National journal of integrated research in medicine, 2016

s: Background and Aim: Microbes are notorious for rapidly develop drugs resistant due to gene tra... more s: Background and Aim: Microbes are notorious for rapidly develop drugs resistant due to gene transfer and spontaneous mutation. So their continues surveillance of antibiogram pattern is necessary to detect muti-drugs resistant organisms to improve patients outcome admitted in ICUs. Objective is to detect bacterial organisms causing infection in different ICUs and to know their antibiotic resistance pattern. Methodology: Total 602 different samples were collected from different ICUs and processed for culture, bacterial identification and antibiotic susceptibility testing done according to CLSI recommendation. Results: Out of total 602 samples, 248 (41.02%) were culture positive. The number of isolated Gram negative and Gram positive organisms were 196 (79.03%) and 52 (20.97%) respectively. Most common isolated of Gram negative organism was Pseudomonas spp. 58(23.38%), followed by Acinetobacter spp. 46 (18.55%). While, most common isolated gram positive cocci was coagulase negative s...

Viruses, 2021

The number of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) cases is increasing in... more The number of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) cases is increasing in India. This study looks upon the geographic distribution of the virus clades and variants circulating in different parts of India between January and August 2020. The NPS/OPS from representative positive cases from different states and union territories in India were collected every month through the VRDLs in the country and analyzed using next-generation sequencing. Epidemiological analysis of the 689 SARS-CoV-2 clinical samples revealed GH and GR to be the predominant clades circulating in different states in India. The northern part of India largely reported the ‘GH’ clade, whereas the southern part reported the ‘GR’, with a few exceptions. These sequences also revealed the presence of single independent mutations—E484Q and N440K—from Maharashtra (first observed in March 2020) and Southern Indian States (first observed in May 2020), respectively. Furthermore, this study indicates tha...

International Journal of Medical Science and Public Health, 2015

and Kerala. [4] All ages and both sexes were affected. [4] The disease is characterized by acute ... more and Kerala. [4] All ages and both sexes were affected. [4] The disease is characterized by acute fever with or without chills, headache, nausea, abdominal pain, photophobia, conjunctival injection, skin rash, and disabling arthralgia. The incubation period ranges between 2 and 10 days. The disease usually affects adults as seen in the 2006 epidemic: of the 333 seropositive patients for chikungunya infection, 299 (90%) were aged older than 15 years. [5] Chikungunya originated from the word "kungunyala" (meaning "that which bends up"). The patient with this disease develops a stooped posture because of severe arthritis, typically affecting the wrists, hands, ankles, and feet. The fever and skin rash are short-lasting, but the joint pains may recur or linger for a long Background: Chikungunya is viral fever with the main symptom of joints pain for prolong duration. It persists as major public health problem till date. Objective: To know the seroprevalence, clinical presentation, and seasonal trends of chikungunya infection in a tertiary-care hospital in Ahmedabad, Gujarat, India. Materials and Methods: A retrospective observational study was conducted at a tertiary-care hospital in Ahmedabad. Totally, 2193 blood samples were received from different wards from suspected cases of chikungunya fever and tested for IgM antibody using ELISA in civil hospital Ahmedabad for duration of 1 year (September 2013 to August 2014). Result: Of the 2193 cases tested, 724 (33.01%) were positive for IgM antibodies; 33.28% were aged younger than 30 years and 66.71% older than 30 years. All presented with fever (100%), followed by joint pain, headache, body ache, joint swelling, and rash (93.6%, 73%, 39.7%, and 17.4%, respectively). Maximum cases were reported during September to January (31.98%, 35%, 36.41%, 43.34%, and 42.18%, respectively), with male subjects of 41.02% and female subjects, 58.97%. Conclusion: Seroprevalence of chikungunya in our study (33.01%), which was high in late monsoon and winter, suggests that it continues to be a major health problem in our setup and indicates the need of appropriate strategies to reduce the severity of disease.

Journal of Global Infectious Diseases, 2012

V iral hepatitis, an important world health problem, is responsible for acute infection and chron... more V iral hepatitis, an important world health problem, is responsible for acute infection and chronic sequel. [1] hepatitis B virus (HBV) infection is a common viral disease and the present data show that more than one-third of the world's population is infected with this virus. [2] HBVinfected patients show a variety of clinical symptoms ranging from an apparently healthy inactive carrier state to fulminant hepatitis or chronic liver disease, including cirrhosis and hepatocellular carcinoma. [3,4] More than 2000 million people alive today have been infected with HBV at some time in their lives. [5] It is