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Papers by Dr Jagdish kandpal

Research paper thumbnail of Non-Invasive Prenatal Genetic Screening to Detect Trisomies In Singleton and Twin Pregnancies

International Journal Of Medical Science And Clinical Invention, Oct 12, 2022

Research paper thumbnail of Phage amplification technology for the rapid detect ion of Mycobacterium tuberculosis complex-clinical utility for the diagnosis of pulm onary tuberculosis

Advances in Applied Science Research, 2013

Research paper thumbnail of Prevalence of hepatitis B virus genotypes and sub-genotypes in north and east regions of India: DNA sequencing methodology

Current Trends in Biotechnology and Pharmacy, Jul 29, 2020

Introduction: Hepatitis B virus (HBV) is a highly prevalent infecting virus among liver-related d... more Introduction: Hepatitis B virus (HBV) is a highly prevalent infecting virus among liver-related diseases. The genetic distribution and identification of HBV genotypes and sub-genotypes represented a challenge to control the spread of infection. To find out molecular prevalence, the present methodology was carried out for the distribution pattern of HBV genotypes and subgenotypes in north and east regions of India. Methods:A total of 67 HBV DNA positive subjects were studied. At first, the DNA samples for HBV positive cases were screened by Real-time PCR, and then the selected region of HBV polymerase gene was amplified for the sequence analysis to determine genotypes and sub-genotypes. Results:The prevalent genotype found was the genotype D (62.68 %), followed by genotype A (29.85 %), and genotype C (7.46 %). Subgenotype C1 was identified in the east region only. The frequency of sub-genotype A1 was higher in the north region (n = 13, 30.95 %) followed by east region (n = 7, 28 %). HBV sub-genotype D1 was found to be predominant in 15 (35.71 %) subjects followed by sub-genotypes D2 in 10 (23.80 %) subjects from the north region. HBV sub-genotype D2 was found to be predominant in 8 (32 %) subjects from the east region. Conclusions:In conclusion, the method clearly demonstrates the high prevalence of subgenotypes D1, D2, A1 in this region. Also, the identification of the sub-genotype C1 in the east region emphasizes the high transmission infection risk and transmission route towards other regions of India.

Research paper thumbnail of 30. DNA prevalence of hepatitis B virus infection in the suspected population of Indian regions

Journal of clinical and experimental hepatology, Jul 1, 2018

Research paper thumbnail of Non-Invasive Prenatal Genetic Screening to Detect Trisomies In Singleton and Twin Pregnancies

International Journal of Medical Science and Clinical Invention

Background Screening for fetal anomalies is one of the most critical parts of prenatal testing to... more Background Screening for fetal anomalies is one of the most critical parts of prenatal testing to reduce the burden of chromosomal aberrations and improve clinical outcomes in pregnancy. The available literature suggests that India carries a strong economic, emotional, and health care burden of new-born genetic diseases. Material and Methods: We present a single-center retrospective study of 200 singletons and 6 dichorionic diamniotic twin gestations screened for chromosomal abnormalities and trisomies using NIPT. The patients were presented with a gestation period of 10-12 (plus a few days) weeks, clinically confirmed with singleton or diamniotic dizygotic twins, who underwent NIPT between the years 2021-2022 (mid) at our center. All the patients underwent sonographic examination and biochemical investigations with regular follow-up and genetic counseling. The clinical history and family history of the presence of any genetic disease or any other disease were previously taken by t...

Research paper thumbnail of Phage amplification technology for the rapid detect ion of Mycobacterium tuberculosis complex-clinical utility for the diagnosis of pulm onary tuberculosis

Advances in Applied Science Research, 2013

Rapid, low cost and sensitive methods play signific ant role in the treatment and management of T... more Rapid, low cost and sensitive methods play signific ant role in the treatment and management of Tubercu losis. Study includes utility of Phage Amplification Technology in parallel with in-house mpb-64 based PCR, microscopy, and BACTEC 460 Tuberculosis culture method for Mycobact erium Tuberculosis complex detection. Six hundred pulmonary specimens which include 300 sputum and 30 0 bronchial alveolar lavage (BAL) specimens), were considered for the study. The sensitivity, of AFB s mear, PCR and FAST Plaque Tuberculosis (FPA) assay for the BAL were 50.9%, 90.2% and 88.67%respectively. While in case of sputum specimens the sensitivity, of AF B smear, PCR method and FASTPlaque TB assay test were 58.25% , 87.37%, 85.85% respectively. In conclusion, FPA p roved to be sensitive, cheap, relative to the PCR and rap id than the culture. It gives result within 48 hour s comparative to the culture which takes 2-6 weeks for the detection . In contrast to PCR, where it can not differentiat e dead...

Research paper thumbnail of Hepatitis B Virus Drug Resistance and Surface Gene Escape Mutation in India: Association with Genotype and Viral Load

The Hepatitis B virus (HBV), reverse transcriptase (RT) region of the polymerase gene is largely ... more The Hepatitis B virus (HBV), reverse transcriptase (RT) region of the polymerase gene is largely overlapped by the surface gene (HBsAg). In-spite the high conservation of HBVRT, infrequent mutations has been described. The aim of the study was to identify mutation for drug resistance within RT and HBsAg escape region, with respective of viral load and genotype in the Indian subcontinent. The study was done for 87 individual (mean age 33.44 ±15.04 years), between Apr 2017 to May 2018. HBV DNA of an infected individual was ranged, 608 to >7.0 ×10 IU/ ml (median log 6.41 log 10). Sub-genotyping and mutation determination was done in the amplified reverse transcriptase region of the HBV polymerase gene. Sub-genotype C1 (10.16%) and D5 (6.77%) was identified only in higher viral load cases. The frequency of HBV drug resistance mutation was 11.53% (3/26) for each sub-genotype D1, D2 and 9.52% (2/21) for sub-genotype A1. The drug resistance mutation was related to LAM (80V), ADV (236T, ...

Research paper thumbnail of Prevalence of hepatitis B virus genotypes and sub-genotypes in north and east regions of India: DNA sequencing methodology

Current Trends in Biotechnology and Pharmacy, 2020

Research paper thumbnail of Dna Prevalence of Hepatitis B Virus Infection Across Different Demographics in Indian Population

Introduction: Diagnosis has been the major aspects of liver-related infection, for providing earl... more Introduction: Diagnosis has been the major aspects of liver-related infection, for providing early advice to patients. Data about the hepatitis B virus (HBV) DNA level among the Indian population were limited. Objectives: To the best of HBV diagnosis, the present study was conducted to determine the virus DNA level and the frequency of infection among the age group and genders in the Indian region. Methods: A total of 926 suspected subjects, 520 (56.15%) male and 406 (43.84%) female, from east (n = 370), north (n = 410), south (n = 45) and west (n = 101) of ages between 6 and 75 years (mean age 39.65 ± 15.20 years) included in this study. Viral DNAs were isolated from the plasma and performed with the artus® HBV RG PCR assay for HBV specific target. Results: HBV DNA was identified in a total of 636/926 (68.68%) subjects; 367 (70.57%) male and 269 (66.25%) female. Demographic data evaluation of viral infection among genders within different regions and age groups indicated no signifi...

Research paper thumbnail of Real Time PCR Usage in the Quantification of Hepatitis B Virus DNA-Clinical Applications in Disease Management

Simultaneous quantification and detection of Hepatitis B virus (HBV) DNA plays significant role i... more Simultaneous quantification and detection of Hepatitis B virus (HBV) DNA plays significant role in diagnosing and monitoring infection related to HBV as well as assessing therapeutic response. Variability among HBV genotypes and the huge range of clinical HBV DNA levels presents challenges for PCR-based amplification techniques. High sensitivity, wide linear range, good reproducibility, and genotype inclusivity, combined with a small sample volume requirement and low cost, make this novel quantitative HBV Real-Time PCR assay particularly well suited for application to large clinical and epidemiological studies. Serum DNA levels are a prognostic factor, and contribute to define the phase of chronic hepatitis B (CHB) infection, the treatment indication, and allow an assessment of the efficacy of antiviral therapy. High levels of HBV DNA are an independent risk factor for cirrhosis and hepatocellular carcinoma HCC in Asia. Recent advances in antiviral therapy, based on the development ...

Research paper thumbnail of 30. DNA prevalence of hepatitis B virus infection in the suspected population of Indian regions

Journal of Clinical and Experimental Hepatology, 2018

Research paper thumbnail of 31. Viral load dynamics of hepatitis B virus DNA infection among diverse group of India

Journal of Clinical and Experimental Hepatology, 2018

Research paper thumbnail of Tuberculosis and Molecular Diagnosis

Biotechnology (Reading, Mass.)

The incidence of Tuberculosis varies considerably around the world and most Mycobacterial infecti... more The incidence of Tuberculosis varies considerably around the world and most Mycobacterial infections in developing nations are still being caused by Mycobacterium tuberculosis members. A quick and correct diagnosis is of great importance because of the high morbidity. Unfortunately, conventional bacteriological methods are time consuming, their sensitivity is low, and so treatment occasionally becomes empirical. PCR method has high specificity in identifying M. tuberculosis in various specimens. Molecular diagnostic tools for Tuberculosis (TB) have evolved quickly with new innovations which can provide unprecedented opportunities for the rapid, sensitive and specific diagnosis of M. tuberculosis in clinical specimens and the status of its drug sensitivity. Microscopy and culture methods can not be replaced but the molecular assays can be applied in parallel with any new molecular tests for the diagnosis of TB. For extra pulmonary specimens, the use of the amplification methods is ad...

Research paper thumbnail of Nondisjunction in trisomy 21: Origin and mechanisms

Cytogenetic and Genome Research, 2000

Chromosomal aneuploidy is a fundamental characteristic of the human species. In this review we su... more Chromosomal aneuploidy is a fundamental characteristic of the human species. In this review we summarize the knowledge about the origin and mechanisms of nondisjunction in human trisomy 21 that has accumulated during the last decade by using DNA polymorphism analysis. The first molecular correlate of nondisjunction in humans is altered recombination, meiosis I errors being associated with reduced recombination and maternal meiosis II errors with increased recombination between the nondisjoined chromosomes. Thus, virtually all maternal meiotic errors of chromosome 21 seem to be initiated in meiosis I. Advanced maternal age remains the only well documented risk factor for maternal meiotic nondisjunction, but there is, however, still a surprising lack of understanding of the basic mechanisms behind the maternal age effect.

Research paper thumbnail of Non-Invasive Prenatal Genetic Screening to Detect Trisomies In Singleton and Twin Pregnancies

International Journal Of Medical Science And Clinical Invention, Oct 12, 2022

Research paper thumbnail of Phage amplification technology for the rapid detect ion of Mycobacterium tuberculosis complex-clinical utility for the diagnosis of pulm onary tuberculosis

Advances in Applied Science Research, 2013

Research paper thumbnail of Prevalence of hepatitis B virus genotypes and sub-genotypes in north and east regions of India: DNA sequencing methodology

Current Trends in Biotechnology and Pharmacy, Jul 29, 2020

Introduction: Hepatitis B virus (HBV) is a highly prevalent infecting virus among liver-related d... more Introduction: Hepatitis B virus (HBV) is a highly prevalent infecting virus among liver-related diseases. The genetic distribution and identification of HBV genotypes and sub-genotypes represented a challenge to control the spread of infection. To find out molecular prevalence, the present methodology was carried out for the distribution pattern of HBV genotypes and subgenotypes in north and east regions of India. Methods:A total of 67 HBV DNA positive subjects were studied. At first, the DNA samples for HBV positive cases were screened by Real-time PCR, and then the selected region of HBV polymerase gene was amplified for the sequence analysis to determine genotypes and sub-genotypes. Results:The prevalent genotype found was the genotype D (62.68 %), followed by genotype A (29.85 %), and genotype C (7.46 %). Subgenotype C1 was identified in the east region only. The frequency of sub-genotype A1 was higher in the north region (n = 13, 30.95 %) followed by east region (n = 7, 28 %). HBV sub-genotype D1 was found to be predominant in 15 (35.71 %) subjects followed by sub-genotypes D2 in 10 (23.80 %) subjects from the north region. HBV sub-genotype D2 was found to be predominant in 8 (32 %) subjects from the east region. Conclusions:In conclusion, the method clearly demonstrates the high prevalence of subgenotypes D1, D2, A1 in this region. Also, the identification of the sub-genotype C1 in the east region emphasizes the high transmission infection risk and transmission route towards other regions of India.

Research paper thumbnail of 30. DNA prevalence of hepatitis B virus infection in the suspected population of Indian regions

Journal of clinical and experimental hepatology, Jul 1, 2018

Research paper thumbnail of Non-Invasive Prenatal Genetic Screening to Detect Trisomies In Singleton and Twin Pregnancies

International Journal of Medical Science and Clinical Invention

Background Screening for fetal anomalies is one of the most critical parts of prenatal testing to... more Background Screening for fetal anomalies is one of the most critical parts of prenatal testing to reduce the burden of chromosomal aberrations and improve clinical outcomes in pregnancy. The available literature suggests that India carries a strong economic, emotional, and health care burden of new-born genetic diseases. Material and Methods: We present a single-center retrospective study of 200 singletons and 6 dichorionic diamniotic twin gestations screened for chromosomal abnormalities and trisomies using NIPT. The patients were presented with a gestation period of 10-12 (plus a few days) weeks, clinically confirmed with singleton or diamniotic dizygotic twins, who underwent NIPT between the years 2021-2022 (mid) at our center. All the patients underwent sonographic examination and biochemical investigations with regular follow-up and genetic counseling. The clinical history and family history of the presence of any genetic disease or any other disease were previously taken by t...

Research paper thumbnail of Phage amplification technology for the rapid detect ion of Mycobacterium tuberculosis complex-clinical utility for the diagnosis of pulm onary tuberculosis

Advances in Applied Science Research, 2013

Rapid, low cost and sensitive methods play signific ant role in the treatment and management of T... more Rapid, low cost and sensitive methods play signific ant role in the treatment and management of Tubercu losis. Study includes utility of Phage Amplification Technology in parallel with in-house mpb-64 based PCR, microscopy, and BACTEC 460 Tuberculosis culture method for Mycobact erium Tuberculosis complex detection. Six hundred pulmonary specimens which include 300 sputum and 30 0 bronchial alveolar lavage (BAL) specimens), were considered for the study. The sensitivity, of AFB s mear, PCR and FAST Plaque Tuberculosis (FPA) assay for the BAL were 50.9%, 90.2% and 88.67%respectively. While in case of sputum specimens the sensitivity, of AF B smear, PCR method and FASTPlaque TB assay test were 58.25% , 87.37%, 85.85% respectively. In conclusion, FPA p roved to be sensitive, cheap, relative to the PCR and rap id than the culture. It gives result within 48 hour s comparative to the culture which takes 2-6 weeks for the detection . In contrast to PCR, where it can not differentiat e dead...

Research paper thumbnail of Hepatitis B Virus Drug Resistance and Surface Gene Escape Mutation in India: Association with Genotype and Viral Load

The Hepatitis B virus (HBV), reverse transcriptase (RT) region of the polymerase gene is largely ... more The Hepatitis B virus (HBV), reverse transcriptase (RT) region of the polymerase gene is largely overlapped by the surface gene (HBsAg). In-spite the high conservation of HBVRT, infrequent mutations has been described. The aim of the study was to identify mutation for drug resistance within RT and HBsAg escape region, with respective of viral load and genotype in the Indian subcontinent. The study was done for 87 individual (mean age 33.44 ±15.04 years), between Apr 2017 to May 2018. HBV DNA of an infected individual was ranged, 608 to >7.0 ×10 IU/ ml (median log 6.41 log 10). Sub-genotyping and mutation determination was done in the amplified reverse transcriptase region of the HBV polymerase gene. Sub-genotype C1 (10.16%) and D5 (6.77%) was identified only in higher viral load cases. The frequency of HBV drug resistance mutation was 11.53% (3/26) for each sub-genotype D1, D2 and 9.52% (2/21) for sub-genotype A1. The drug resistance mutation was related to LAM (80V), ADV (236T, ...

Research paper thumbnail of Prevalence of hepatitis B virus genotypes and sub-genotypes in north and east regions of India: DNA sequencing methodology

Current Trends in Biotechnology and Pharmacy, 2020

Research paper thumbnail of Dna Prevalence of Hepatitis B Virus Infection Across Different Demographics in Indian Population

Introduction: Diagnosis has been the major aspects of liver-related infection, for providing earl... more Introduction: Diagnosis has been the major aspects of liver-related infection, for providing early advice to patients. Data about the hepatitis B virus (HBV) DNA level among the Indian population were limited. Objectives: To the best of HBV diagnosis, the present study was conducted to determine the virus DNA level and the frequency of infection among the age group and genders in the Indian region. Methods: A total of 926 suspected subjects, 520 (56.15%) male and 406 (43.84%) female, from east (n = 370), north (n = 410), south (n = 45) and west (n = 101) of ages between 6 and 75 years (mean age 39.65 ± 15.20 years) included in this study. Viral DNAs were isolated from the plasma and performed with the artus® HBV RG PCR assay for HBV specific target. Results: HBV DNA was identified in a total of 636/926 (68.68%) subjects; 367 (70.57%) male and 269 (66.25%) female. Demographic data evaluation of viral infection among genders within different regions and age groups indicated no signifi...

Research paper thumbnail of Real Time PCR Usage in the Quantification of Hepatitis B Virus DNA-Clinical Applications in Disease Management

Simultaneous quantification and detection of Hepatitis B virus (HBV) DNA plays significant role i... more Simultaneous quantification and detection of Hepatitis B virus (HBV) DNA plays significant role in diagnosing and monitoring infection related to HBV as well as assessing therapeutic response. Variability among HBV genotypes and the huge range of clinical HBV DNA levels presents challenges for PCR-based amplification techniques. High sensitivity, wide linear range, good reproducibility, and genotype inclusivity, combined with a small sample volume requirement and low cost, make this novel quantitative HBV Real-Time PCR assay particularly well suited for application to large clinical and epidemiological studies. Serum DNA levels are a prognostic factor, and contribute to define the phase of chronic hepatitis B (CHB) infection, the treatment indication, and allow an assessment of the efficacy of antiviral therapy. High levels of HBV DNA are an independent risk factor for cirrhosis and hepatocellular carcinoma HCC in Asia. Recent advances in antiviral therapy, based on the development ...

Research paper thumbnail of 30. DNA prevalence of hepatitis B virus infection in the suspected population of Indian regions

Journal of Clinical and Experimental Hepatology, 2018

Research paper thumbnail of 31. Viral load dynamics of hepatitis B virus DNA infection among diverse group of India

Journal of Clinical and Experimental Hepatology, 2018

Research paper thumbnail of Tuberculosis and Molecular Diagnosis

Biotechnology (Reading, Mass.)

The incidence of Tuberculosis varies considerably around the world and most Mycobacterial infecti... more The incidence of Tuberculosis varies considerably around the world and most Mycobacterial infections in developing nations are still being caused by Mycobacterium tuberculosis members. A quick and correct diagnosis is of great importance because of the high morbidity. Unfortunately, conventional bacteriological methods are time consuming, their sensitivity is low, and so treatment occasionally becomes empirical. PCR method has high specificity in identifying M. tuberculosis in various specimens. Molecular diagnostic tools for Tuberculosis (TB) have evolved quickly with new innovations which can provide unprecedented opportunities for the rapid, sensitive and specific diagnosis of M. tuberculosis in clinical specimens and the status of its drug sensitivity. Microscopy and culture methods can not be replaced but the molecular assays can be applied in parallel with any new molecular tests for the diagnosis of TB. For extra pulmonary specimens, the use of the amplification methods is ad...

Research paper thumbnail of Nondisjunction in trisomy 21: Origin and mechanisms

Cytogenetic and Genome Research, 2000

Chromosomal aneuploidy is a fundamental characteristic of the human species. In this review we su... more Chromosomal aneuploidy is a fundamental characteristic of the human species. In this review we summarize the knowledge about the origin and mechanisms of nondisjunction in human trisomy 21 that has accumulated during the last decade by using DNA polymorphism analysis. The first molecular correlate of nondisjunction in humans is altered recombination, meiosis I errors being associated with reduced recombination and maternal meiosis II errors with increased recombination between the nondisjoined chromosomes. Thus, virtually all maternal meiotic errors of chromosome 21 seem to be initiated in meiosis I. Advanced maternal age remains the only well documented risk factor for maternal meiotic nondisjunction, but there is, however, still a surprising lack of understanding of the basic mechanisms behind the maternal age effect.