Dr.M.A.Badhul Haq - Academia.edu (original) (raw)

Papers by Dr.M.A.Badhul Haq

Thalassas: An International Journal of Marine Sciences

y shoulder girdle (Bigelow and Schroeder 1953; Compagno 1973). These electric rays are generally ... more y shoulder girdle (Bigelow and Schroeder 1953; Compagno 1973). These electric rays are generally distributed from the inshore to deep waters that are found in habitats such as sandy beaches, muddy bays, estuaries, coral reefs, river mouths, and on the upper continental slopes under warmtemperate to tropical continental waters around the world (Carvalho et al. 1999). They are notably absent on the Mediterranean Sea, Eastern Atlantic, SouthEast Pacific and from isolated islands of the Pacific Plate (Carvalho et al. 1999). The electric rays belonging to the Order: Torpediniformes consists of five families viz., Hypnidae, Narcinidae, Narkidae, Platyrhinidae, and Torpedinidae (Last et al. 2016; Nelson et al. 2016) with 73 valid species (Fricke et al. 2023). The electric rays of the Family: Narcinidae is commonly called Numbfishes so far 5 genera Benthobatis, Diplobatis, Discopyge, Narcine, Narcinops (Last et al. 2016) and 31 valid species have been reported (Froese and Pauly 2023). The species occurring under the genus Narcine is easily characterized from the other species of electric rays by the basis of frontal disc shape, arrangement of the mouth, the position of teeth externally on tooth bands, and unique anterior orbital cartilages (Henle 1834; Carvalho et al. 2002a). In India total of 7 species belonging to the family: narcinidae have been recorded and reported which are Benthobatis B.

369-375<span style="mso-ansi-language: EN-GB;mso-fareast-language:EN-GB" lang="... more 369-375<span style="mso-ansi-language: EN-GB;mso-fareast-language:EN-GB" lang="EN-GB">Outbreak of disease is one of the major stumbling blocks in the development and sustainability of aquaculture. White spot syndrome virus (WSSV), the causative virus of the white spot disease, was found in most shrimp farming areas of the world, where it causes large economic losses to the shrimp farming industry. The objective of the present investigation was to determine the level of WSSV transmission from the infected tiger prawn Penaeus monodon to specific pathogen free (SPF) Litopenaeus vannamei in WSSV impact region of Tamil Nadu. In our study, we performed polymerase chain reaction (PCR), histological observation and scanning electron microscopy to detect WSSV. Our nested PCR revealed the bands ranging from 296 to 910 bps in WSSV infected L. vannamei shrimps. The infected shrimp showed 20 to 2000 copies of viral DNA. Histological surveillance was performed on infecte...

IJMS Vol.49(10) [October 2020], Oct 1, 2020

Grouper fishes have been considered as commercially important candidate species for aquaculture. ... more Grouper fishes have been considered as commercially important candidate species for aquaculture. Among them, the Tiger grouper (Epinepheleus fuscoguttatus) is one of the most relished finfish that demands an excellent price in the fish markets of Southeast Asia. Rajiv Gandhi Centre for Aquaculture (RGCA), has launched a project on breeding, seed production and grow out farming of grouper at Andaman & Nicobar Islands (ANI). The project developed technology for breeding and grow-out farming of Tiger grouper, which is widely distributed in Andamans and also form a candidate species for captive breeding and sea cage culture. Its wild catches are extremely limited and insufficient to meet the huge market demands. Hence, development of a standard technology for the seed production of Tiger grouper is an imperative to boost the country's export revenue. The paper presented here, is one such initiative by RGCA on seed production and cage culture of E. fuscoguttatus.

Progress over the past years has revealed much strength of the ornamental fish as an alternative ... more Progress over the past years has revealed much strength of the ornamental fish as an alternative model for the environmental sideline in the aquaculture both in fresh water and marine waters. These include low rearing costs, an earlier life stage. Aquarium fish are one of the largest groups of pets in the world. There is an increasing demand for the veterinary services that are related to the ornamental fish. Early clinical signs in many infectious and noninfectious diseases of fish are manifested by the skin. More diseases in fish have been described and have been associated with the research for the future has been discussed. This article also describes the more common environmental and pathogen related dermatologic diseases of the aquarium fishes. Disease prevention, control and various treatment methods also have been discussed.

The objective of the present investigation was to determine the level of WSSV transmission from t... more The objective of the present investigation was to determine the level of WSSV transmission from the infected tiger prawn Penaeus monodon to SPF Litopenaeus vannamei in laboratory captivity condition, in connection with to PCR detection and viral genome sequence. The nested PCR product band was ranged between 296 to 910 bp and was found during entire period from the experimental studies of SPF L.vannamei shrimps. WSSV was used as an out group for this phylogeny analysis. The constructed Phylogram showed healthy Inter-specific variations as the clades of other geographical gene were clearly segregated. Nucleotide composition of the test organisms showed average amount of G+C content (~47.0%). G+C content of the WSSV genome were analyzed and there was a little fluctuation in the G+C content of white spot syndrome which differed from the other nucleotide composition of test organism. M.A.BADHUL HAQ

Abundance of anemone fishes in North Bay Island and mass culture of live food

Bioactive peptides isolated from various fish protein hydrolysates have shown a numerous bioactiv... more Bioactive peptides isolated from various fish protein hydrolysates have shown a numerous bioactivities such as antihypertensive, antithrombotic, immunomodulatory and antioxidative activities. Fish protein hydrolysate extracts performs the regulation of the immune system, gastrointestinal functions, blood pressure, glucose inhibitory activity, antihypertensive effect, antioxidant properties and angiotensin converting enzyme inhibitory activity. Fish waste is rich in potentially valuable oils, minerals, enzymes, pigments and flavors etc. These may also have many alternative uses in food, pharmaceutical, agricultural, aquaculture and industrial applications. Fish proteins can have not only nutritional but also functional and biological applications. Intake of fish oil, which is an excellent source of omega- 3 fatty acids, has been linked to promotion of human health to fight against numerous diseases. Peptides derived from fish proteins have shown the ability of exerting potent antioxi...

International Journal of Pure & Applied Bioscience

The preparation viral inoculums were used in this study was isolated from infected polychaete P. ... more The preparation viral inoculums were used in this study was isolated from infected polychaete P. cultrifera from the experimental tank. Viral DNA was isolated from purified virions by treatment with proteinase K followed by phenol and chloroform extraction and dialysis against TE. The inoculum (viral DNA) was used to challenge WSSV-negative animals under experimental conditions. Triplicate tanks were maintained to each species shrimp with oral route and intramuscular injection inoculation. Tissues and hemolymph were collected from experimental shrimps for PCR analysis. Shrimps were sacrificed at 24h, 48h, 3, 6, 12, 18 and 25 days interval. PCR analysis showed the appearance of a prominent band of PCR amplified product of WSSVDNA at 848 bp at 24 hr up to day 7 of post infection in the pleopods although these band 296 bp (200 copies) continued from day 8 to 12. On days 13 and 14 the product band observed as 650 bp (2000 copies) and an equal amount of product band of 910 bp (20,000) during the days 15 to 18. The same product band was ranged between 296 to 910 bp during 19 to 25 days in the L. vannamei shrimps which exposed to WSSV by oral route. No band was observed in the control group. Histological observation of low, moderate and severely WSSV infected L. vannamei shrimps were revealed degenerated cells characterized by basophilic intranuclear inclusions in the tissues of WSSV infected mid-gut gland, lymphoid organ, gill lamellae, gut epithelium tissue of L. vannamei on the 9 th day of post-inoculum. The survival days of L. vannamei extends up to 23 days for oral rooted and 24 days for intramuscularly injected shrimps of which mortality rate on the concerning days was 93.30% and 90.0 % respectively.

Characterization and propensity of white spot syndrome virus extracted from imported specific pat... more Characterization and propensity of white spot syndrome virus extracted from imported specific pathogen free (SPF) pacific Litopenaeus vannamei brooders progeny by performing SF9 cell line culture

Indian Journal of Biotechnology, 2015

Outbreak of disease is one of the major stumbling blocks in the development and sustainability of... more Outbreak of disease is one of the major stumbling blocks in the development and sustainability of aquaculture. White spot syndrome virus (WSSV), the causative virus of the white spot disease, was found in most shrimp farming areas of the world, where it causes large economic losses to the shrimp farming industry. The objective of the present investigation was to determine the level of WSSV transmission from the infected tiger prawn Penaeus monodon to specific pathogen free (SPF) Litopenaeus vannamei in WSSV impact region of Tamil Nadu. In our study, we performed polymerase chain reaction (PCR), histological observation and scanning electron microscopy to detect WSSV. Our nested PCR revealed the bands ranging from 296 to 910 bps in WSSV infected L. vannamei shrimps. The infected shrimp showed 20 to 2000 copies of viral DNA. Histological surveillance was performed on infected L. vannamei at cephalothorax region. We observed intranuclear inclusion bodies characteristic in the gill regi...

Imperial journal of interdisciplinary research, 2016

: The shrimp industries make a remarkable economic development in the aquaculture industries in I... more : The shrimp industries make a remarkable economic development in the aquaculture industries in India. The task behaving the forming is the viral contaminations in the cultural industries. India was means with pre diagnosis carried mostly against WSSV and very less enquiries on IHHNV. This review article deals on major DNA viruses White Spot Syndrome virus (WSSV) , Monodon Baculovius (MBV), Infectious Hypodermal and Hematopoietic Necrosis virus (IHHNV) and Hepatopancreatic Parvovirus (HPV) with making an details molecular understanding and diagnostic awareness.

Introduction The application of scientific knowledge for the development of fishing industry lies... more Introduction The application of scientific knowledge for the development of fishing industry lies in an intimate knowledge on the biology of fishes. Without the information on the life history, habits and behavior of fishes, it is not possible to plan, control and manage the fishery resources in a satisfied manner. The food and feeding habits of fishes is in higher concern for the fishery biologists. The stomach content analyses are said to be an important parameter in the studies on fish biology. Such studies help to gain information on the main prey organisms and the preference or dietary overlap between year classes or different species living in the same or comparable habitats. Such studies allow determining the seasonal and geographical variations in dietary composition to discern the diet rhythm in feeding behavior and to estimate the energy resources and to help in modeling energy flow in marine ecosystem. In addition, the quality and quantity of food is one of the critical d...

Archives of Applied Science Research, 2011

In the recent years there has been an increased research interest to develop cardio protective an... more In the recent years there has been an increased research interest to develop cardio protective and other pharmacological therapies from natural sources like animals and plants from marine origin. Fish proteins extracts and their and their hydrolysates are the best protein compounds in terms of nutrition and other health promoting benefits. The fish extracts are studied to larger extents and said has been proved that they contain promising health benefit molecules. Chemicals produced by or found in marine organisms have been shown to have a wide variety of applications as pharmaceuticals for humans and other animals. Several studies have stated that the compounds of fish muscle have antioxdative properties which include ascorbic acid, uric acid, glutathione, polyamines, histidine containing dipeptides and free amino acids Uses have included antibacterial, and antiviral and anti-tumor agents. Particular attention has been paid to a variety of toxins formed by marine organisms. Nutrace...

International journal of pharma and bio sciences, 2012

The measurement of some proximate profiles such as protein contents, lipids and moisture contents... more The measurement of some proximate profiles such as protein contents, lipids and moisture contents in fish by products is often necessary to ensure that they meet the requirements of food regulations in nutrition aspects and commercial specifications. Minerals are essential nutrients, they are components of many enzymes and metabolism, and contribute also to the growth of the fish. The human body usually contains small amount of these minerals and the deficiency in these principal nutritional elements induces a lot of malfunctioning; as it reduces productivity and causes diseases. Fish received increased attention as a potential source of animal protein and essential nutrients for human. It should be considered that fish tissue presents elevated nutritional significance and therefore is a particularly optional dietary module. The objective of this work was to analyze the proximate and mineral composition of twenty edible fishes of parangipettai coast.

A rapid and highly sensitive detection and quantification method for white spot syndrome virus (W... more A rapid and highly sensitive detection and quantification method for white spot syndrome virus (WSSV) was developed using GeneAmp 5700 Sequencer Detection System and SYBR Green chemistry. The RT-PCR mixture contained the fluorescent dye SYBR Green, which exhibited florescence enhancement upon binding to double strand cDNA. About 10 and 10 copies μg of viral DNA load were observed in the juvenile offsprings of shrimps (Litopenaeus vannamei) originated from Hawaii (Hawaii A) and Singapore, respectively. The inocula of known virus content, derived from three different place of origin, viz., Hawaii (HawaiiA), Singapore and Thailand, showed different logs of WSSV copies as 10, 10 and 10 WSSV copies ng at DNA, respectively. In the positive control, a higher amount DNA copies were noticed. An appropriate quantity of 10, 10 and 10 WSSV copies of ng DNA was observed in the juvenile offsprings of shrimp, respectively originated from Hawaii-A, Singapore and Thailand. More importantly, there wa...

A total of 27 isolates of bacteria were isolated from the jelly fish Crambionella stuhlmanni was ... more A total of 27 isolates of bacteria were isolated from the jelly fish Crambionella stuhlmanni was obtained from the Parangipettai fish landing centre, south east coast of India. The viable count of the bacteria in Crambionella stuhlmanni ranged from 10-10 per g. The morphological and, biochemical investigations were done to characterize the isolates. Based on biochemical and morphological identification the isolates were found to be Vibrio, Staphylococcus, Lactobacillus, salmonella and Klebsiella species were predominant. All isolates were primarily screened for amylase production by starch plate method. Among 27 isolates, 2 isolates hydrolyzed the starch on starch agar and only one isolate was selected for further study.

International Journal on Environmental Sciences, 2011

The exponential growth of the human population has led to the accumulation of huge amounts of non... more The exponential growth of the human population has led to the accumulation of huge amounts of non-degradable waste materials across our planet. Living conditions in the biosphere are therefore changing dramatically, in such a way that the presence of non biodegradable residues is affecting the potential survival of many species. The present interest in hydrocarbon biodegradation is motivated in large part by their presence as environmental pollutants. Bioremediation has proven to be an alternative to diminish the effects caused by hydrocarbon pollution of soil and water, using the metabolic capacities of microorganisms that can use hydrocarbons as source of carbon and energy, or that can modify them by co metabolism. The bacteria are well-known for their metabolic diversity and this variety is the fact that many bio-hazardous or relentless anthropogenic chemical compounds are degraded by microbial activities. This review paper highlights the role of various microbes and their deriva...

The changes in water temperature in clown fish Amphiprionocellaris were reared in captive conditi... more The changes in water temperature in clown fish Amphiprionocellaris were reared in captive conditions at different water temperatures. Assisting the physiological and ecological responses of marine fishes to the change in climate on water gives a dramatic response. The results illustrated that water temperature has drastic change on the survival, growth and plasma levels of A. ocellaris. Specific growth rate (SGR), FCR (feed conversion ratio) increased with the increase of water temperature from 23 to 33 o C and sudden decrease was observed at extreme high temperature treatment (37 oC) which was noted as thermal stress in clown fish A. ocellaris. The plasma level concentration at different water temperature also deliberately increased with the increase of temperature. This study put forward that the reduce growth, survival and feeding of juveniles at lower temperature which have ecological impacts on clown fish juveniles in settlement and population replacement in the wild. This stud...

This work was supported by the SERB, Department and Science and Technology Grant no: SR / FT / LS... more This work was supported by the SERB, Department and Science and Technology Grant no: SR / FT / LS-125 / 2011. The authors thank the authorities of Annamalai University for their constant support and encouragement.

The length-weight also helps in predicting the condition, reproductive history and life history o... more The length-weight also helps in predicting the condition, reproductive history and life history of fish species. In the present study, the length - weight relationship in E.malabaricus has been studied with the objectives of provides a set of estimating equations and to determine whether any differences exists in their relationship between males, females and juveniles. The regression parameters of the length - weight relationship of E.malabaricus and the details of sums of squares and products of length - weight data of males, females and juveniles were analyzed. While analyzing of length - weight data of E.malabaricus separately for males, females, and juveniles for the year 2009 and 2010 the regression lines indicated a close relationship without any inflection. Slope values (b value) of males, females and juveniles are centered around 2.05, 2.78 and 2.01 for 2009 and 2.66, 2.80 and 2.35 for 2010 respectively. The correlation coefficients (r) were greater than 0.9. The study obser...