Dr.Naser Alkenani - Academia.edu (original) (raw)

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Papers by Dr.Naser Alkenani

Journal of King Saud University - Science, 2022

Structural Engineering and Mechanics, 2021

List of top 10 significant genes with the highest degree with their general centrality p... more List of top 10 significant genes with the highest degree with their general centrality parameters obtained from network analysis.</p

Journal of Natural Pesticide Research

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Epigenetic alterations are associated with human cancer development and the inactivation of tumor... more Epigenetic alterations are associated with human cancer development and the inactivation of tumor suppressor genes. Two such tumor suppressor genes, PCNA and RB1, showed an aberrant gene expression in HCC. DNA methylation alters the expression of genes and is one of the processes that transform normal cells into cancer cells. PCNA and RB1 genes screened methylation in the promoter regions in HepG2 cells. The current study examined the effect of DNA methyltransferase inhibitor (5-Azacytidine) on gene expression of PCNA and RB1 genes after treatment and showed the impact of 5-aza-CR on the Methylation degree of HepG2 cells. HepG2 cell line originated from hepatocellular carcinoma (HCC). Since HepG2 exhibits the characteristics of human liver carcinoma, it was a good model for detecting the changes in methylation patterns and the gene expression level that was detectable in a clinical setting. The human HepG2 cell line was treated with 5, 10, and 25 µM of 5-aza-CR for 24 h, 48 h, and 72 h. Methylation of PCNA and RB1 was detected by methylation-specific polymerase chain reaction (MSP). PCNA and RB1 gene expression detected by reverse transcription-polymerase chain reaction. The influence of 5-aza-CR on Cell viability was assessed by SRB assay for 24 h, 48 h, and 72 h. The IC50 is 20.52 µM for 24 h, 12.6117 µM for 48 h, and 10.63 µM for 72 h after exposure to 5-aza-CR, which showed that 5-aza-CR inhibited the growth of HepG2 cells in a time, and dose-dependent manner. Although other genes may be demethylated due to the 5-aza-CR treatment, we concentrated on the PCNA and RB1 genes. In HepG2 cells, PCNA and RB1 gene methylation were found before 5-aza-CR treatment. In contrast, no PCNA or RB1 gene expression was detected. Treatment with different concentrations of 5-aza-CR significantly decreased the methylation degree of the PCNA and RB1. 5-aza-CR at 25 µM for 72h showed the highest induction activity of PCNA and RB1 gene expression. Methylation-specific PCR results showed that 5-aza-CR promoted the expression of PCNA and RB1 by demethylation. Our results illustrate that 5-aza-CR could reverse the abnormal methylation degree of the PCNA and RB1 genes that are hypermethylated in HepG2 cells and induces the expression of the PCNA and RB1 genes by demethylation.

Saudi Journal of Biological Sciences, 2017

Vector borne diseases remain the major source of illness and death worldwide. Aedes aegypti is th... more Vector borne diseases remain the major source of illness and death worldwide. Aedes aegypti is the primary carrier of dengue and dengue haemorrhagic fever in many developing countries in the tropical world. Because A. aegypti populations are becoming more and more resistant to conventional and non conventional insecticides, alternative strategies have to be rapidly implemented in the future for dengue vector control. The present study aimed to evaluate the larvicidal efficacy of slow-release formulations (SRFs) of bacterial insecticide Bactimos briquets blended with tow insect growth regulators (IGRs), Altosid XR-briquets and Dudim DT tablet respectively, against mosquito larvae of A. aegypti the primary vector of dengue fever in Jeddah governorate, Saudi Arabia. Semi-field trials were conducted at dengue mosquito research station,

Control Network and Disease Network represent significant changes with strong altered co... more Control Network and Disease Network represent significant changes with strong altered connections of network connectivity from normal to obese state. The average connectivity of nodes in the control state is 15.42, and it has decreased to 14.75 in disease state depicts the overall loss in the interaction in obese condition.</p

SPIN, developed from HINNF... more SPIN, developed from HINNF and their first level interacting partners.</p

Genes enriched are more closed to inflammatory diseases and pathways.</p

A) Genes with their number of functional partners in obese and normal conditions, B) The... more A) Genes with their number of functional partners in obese and normal conditions, B) The fold change of these connected genes to the family of NF-κB proteins.</p

An overview of the top 10 significant genes gene pairs with the highest absolute <i&g... more An overview of the top 10 significant genes gene pairs with the highest absolute DPCC score.</p

International Journal of Biomathematics, Apr 28, 2022

Archives Of Pharmacy Practice, 2021

Ecotoxicology and Environmental Safety, 2019

Cadmium (Cd) is a toxic heavy metal and an abiotic stressor to plants; however, inoculation of en... more Cadmium (Cd) is a toxic heavy metal and an abiotic stressor to plants; however, inoculation of endophytic bacteria can raise resistance in plants against Cd, as well as improve plant growth. In the present study, two endophytic bacterial strains were isolated from Solanum nigrum, identified as Serratia sp. IU01 and Enterobacter sp. IU02 by 16S DNA sequencing. Both IU01 and IU02 were tolerant up to 9.0 mM of Cd in culture broth and successive increase in Cd concentration from 0 mM to 9.0 mM, led to an increase in the SOD enzyme activity of the isolates. Both strains were capable of indole-3-acetic acid (IAA) synthesis and phosphate solubilization, detected through gas spectrometry-mass chromatography (GC-MS) and Pikovskaya agar medium respectively. Brassica juncea plants stressed with 0-25 mg/kg Cd showed retardation in all growth attributes, however, inoculation of strain IU01 and IU02 significantly promoted the plant growth attributes as compared to control. Moreover, antioxidant enzymes and metabolites against reactive oxygen species (ROS) including polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), alcohol dehydrogenase (ADH), reduced glutathione (GSH), malondialdehyde (MDA), flavonoid and polyphenolic contents were also significantly relieved by inoculation of IU01 and IU02 in plant exposed to different concentration of Cd stress as compared to control plants. Phytohormone production, phosphate solubilization, and/or antioxidative support of IU01 and IU02 might be responsible for growth promotion and Cd resistance in the plant.

Plants, 2021

Apricot (Prunus armeniaca L.) is an important temperate fruit crop worldwide. The availability of... more Apricot (Prunus armeniaca L.) is an important temperate fruit crop worldwide. The availability of wild apricot germplasm and its characterization through genomic studies can guide us towards its conservation, increasing productivity and nutritional composition. Therefore, in this study, we carried out the genomic characterization of 50 phenotypically variable accessions by using SSR markers in the erstwhile States of Jammu and Kashmir to reveal genetic variability among accessions and their genetic associations. The genetic parameter results revealed that the number of alleles per locus (Na) ranged from 1 to 6 with a mean Na value of 3.89 and the mean effective number of alleles (Ne) per locus 1.882 with a range of 1.22 to 2. Similarly, the polymorphic information content (PIC) values ranged from 0.464 to 0.104. The observed heterozygosity (Ho) (0.547) was found to have higher than expected heterozygosity (He) (0.453) with average heterozygosity of 0.4483. The dendrogram clustered g...

International Journal of Drug Development and Research

Membrane Water Treatment, 2020

The present research was undertaken to study the synergistic effect of 3 pyrethroid and 3 organop... more The present research was undertaken to study the synergistic effect of 3 pyrethroid and 3 organophosphorus insecticides against field strain of the females and the 2nd larval instars of the housefly, M. domestica using CDC bottle and dipping application bioassay techniques under laboratory conditions, respectively. It was found that pyrethroid insecticides, Cypermethrin and Cyfluthrin showed the highest co-toxicity factor 14.4, while the lowest co-toxicity factor was 1.5. Whereas, the preliminary toxicity screening of the three organophosphorus insecticides (Diazinon, Propetamphos and Pirimiphos methyl) against M. domestica 2nd larval instar showed additive effects with different degrees according to the estimated co-toxicity factor. The mixture of Diazinon and Pirimiphos methyl showed the highest co-toxicity factor 8.10. So it is concluded that mixture of these chemicals has better insecticidal effect against the housefly compare to alone and can be used for the control of housefly.