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Papers by Dr. Rubina Noreen

Research paper thumbnail of Effect of Endophytic Pseudomonas Aeruginosa and Trichoderma Harzianum on Soil-Borne Diseases , Mycorrhizae and Induction of Systemic Resistance in Okra Grown in Soil Amended with Vernonia Anthelmintica ( L . ) Seed ’ S Powder

Biostimulants are used in agricultural practices for plant growth improvement. These fertilizers ... more Biostimulants are used in agricultural practices for plant growth improvement. These fertilizers improve microbial activity and cause a negative impact on soil-borne pathogens. In recent years, stimulating plant’s natural defense is considered as most promising alternative strategy for crop productivity. The present study was carried out to examine the effect of endophytic Pseudomonas aeruginosa and Trichoderma harzianum in soil amendment with Vernonia anthelmintica seed’s powder, on root rotting fungi, plant growth, mycorrhizal population around roots, phosphorous uptake and stimulation of plant defense markers like poylphenol and antioxidant status in okra. Combine application of Vernonia with Pseudomonas aeruginosa and Trichoderma harzianum significantly (p<0.05) suppressed Rhizoctonia solani and Fusarium oxysporum with complete reduction of Macrophomina phaseolina and Fusarium solani. Pseudomonas aeruginosa and T. harzianum alone or in Vernonia amended soil significantly redu...

Research paper thumbnail of Effects of fungicides and storage temperature in maintaining the shelf life and fruit quality of stored mango (Mangifera indica L.)

Pakistan Journal of Botany, 2021

Research paper thumbnail of Impact of Endo-Nodule Fluorescent Pseudomonas and Rhizobia on Root Rotting Fungi and Growth of Soybean (Glycine Max L. Merr)

Rhizobia and fluorescent Pseudomonas associated with root nodules of mungbean (Vigna radiata) wer... more Rhizobia and fluorescent Pseudomonas associated with root nodules of mungbean (Vigna radiata) were studied for their synergistic effect on disease suppression and promotion of plant growth. Fluorescent Pseudomonas viz., NAFP-19, NAFP-31 and NAFP-32 and Rhizobium vignae (NFB-103), R. vignae (NFB-107) and R. vignae (NFB109) were applied simultaneously in naturally infested soil in screen house experiment caused suppression of root rotting fungi of soybean as compared to untreated control. Combined application of both bacteria produced healthier plants than untreated plants or plants inoculated with either component alone.

Research paper thumbnail of Role of Mungbean Root Nodule Associated Fluorescent Pseudomonas and Rhizobia in Suppressing the Root Rotting Fungi and Root Knot Nematodes in Chickpea ( C Icer Arietinum L . )

Three isolates each of fluorescent Pseudomonas (NAFP-19, NAFP-31 and NAFP-32) and rhizobia (NFB10... more Three isolates each of fluorescent Pseudomonas (NAFP-19, NAFP-31 and NAFP-32) and rhizobia (NFB103, NFB107 and NFB-109) which were originally isolated from root nodules of mungbean (Vigna radiata) showed significant biocontrol activity in the screen house and under field condition, against root rotting fungi viz., Macrophomina phaseolina, Fusarium solani, F. oxysporum and Rhizoctonia solani evaluated on chickpea. Biocontrol potential of these isolates was also evaluated against Meloidogyne incognita, the root knot nematode. Application of Pseudomonas and rhizobial isolates as a soil drench, separately or mixed significantly reduced root rot disease under screen house and field conditions. Nematode’s penetration in roots was also found significantly less in rhizobia or Pseudomonas treatments used separately or mixed as compared to control. Fluorescent Pseudomonas treated plants produced greater number of nodules per plant than control plants and about equal to rhizobia treated plants...

Research paper thumbnail of Protective role of epiphytic fluorescent Pseudomonas on natural postharvest decay of tomato at room temperature

Journal of applied botany and food quality, 2017

Following harvest, tomato fruits are susceptible to attack by many fungal pathogens, but healthy... more Following harvest, tomato fruits are susceptible to attack by many fungal pathogens, but healthy fruits may also harbor beneficial microflora, which can delayed the spoilage of fruit. In this study, 30 isolates of epiphytic fluorescent Pseudomonas were isolated from healthy fruits of lemon, melon, grapefruit, tomato and orange. Twelve isolates were identified on molecular basis by amplifying 16S rDNA using a genus-specific primer set PA-GS-F 5’-GACGGGTGAGTAATGCCTA-3’ and PA-GS-R F 5’-CACTGGTGTTCCTTCCTATA-3’ on a conserved sequence of the genus Pseudomonas with a product size ~618 bp. Three potential isolates were examined for their ability to delayed the postharvest natural spoilage and maintained the physiochemical properties during storage for fifteen days in season 2013 and 2014. All three isolates showed promising control of postharvest diseases of tomato in comparison with control in both seasons up to fifteen days of storage at room temperature (23±4 oC, Rh 25-70%). The...

Research paper thumbnail of Role of Rhizobia in Suppressing the Root Diseases of Soybean Under Soil Amendment

Planta Daninha, 2019

ABSTRACT: Rhizobia are soil bacteria, characterized by their unique ability to colonize the roots... more ABSTRACT: Rhizobia are soil bacteria, characterized by their unique ability to colonize the roots of leguminous crops, where they form nitrogen fixing nodules. Considerable evidence has been accumulated to identify the benefits associated with use of rhizobia as biocontrol agents against soil-borne pathogens, in addition to biological nitrogen fixation. In this study, out of four rhizobial isolates tested, Bradyrhizobium sp. inhibited the radial growth of all the test fungi viz: Macrophomina phaseolina, Rhizoctonia solani, Fusarium solani and F. oxysporum, while other 3 rhizobial isolates inhibited the growth of at least three fungi. Cell free culture filtrates of rhizobia also showed nematicidal activity by killing second-stage juveniles of Meloidogyne javanica at varying degrees. Application of rhizobial isolates alone or in mustard cake amended soil significantly (p<0.05) by suppression of root rotting fungi and root knot nematode on soybean roots. Rhizobia-treated plants show...

Research paper thumbnail of Role of fluorescent pseudomonas associated with root nodules of mungbean in the induction of nodulation by the rhizobia in mungbean

Pakistan Journal of Botany, 2019

Fluorescent Pseudomonas has been reported to associate with root nodules of a number of plants an... more Fluorescent Pseudomonas has been reported to associate with root nodules of a number of plants and play role in plant disease suppression and increased plant growth. However their role in nodule formation is still unclear. In a gnotobiotic experiment, combined effect of two isolates each of fluorescent Pseudomonas (NAFP-19 and NAFP-32) and rhizobia (Rhizobium vignae (NFB-103) and R. vignae (NFB-109) on nodules of mungbean was evaluated. The results showed an increase in the number of nodule primordia, mature nodules and the concentration of nitrogen and phosphorus in mungbean plants. In screen house experiment, garden soil which was naturally infested with root rotting fungi and rhizobia was used. Dual application of Pseudomonas and rhizobia in the garden soil produced greater number of nodules per plant and higher nitrogen fixation than when rhizobia were used alone. Dual application of bacteria also suppressed root rotting fungi. The results showed that nodule associated fluorescent Pseudomonas played a role in plant-rhizobia symbiosis.

Research paper thumbnail of Evaluation of biocontrol potential of epiphytic yeast against postharvest Penicillium digitatum rot of stored Kinnow fruit (Citrus reticulata) and their effect on its physiochemical properties

Postharvest Biology and Technology, 2019

Twenty five isolates of epiphytic yeasts were isolated from healthy surface of tomato, mango, lem... more Twenty five isolates of epiphytic yeasts were isolated from healthy surface of tomato, mango, lemon, orange, grape fruit and green chili and evaluated for their potential against postharvest Penicillium digitatum decay of kinnow fruits. Identification of yeast was confirmed by molecular biology tools. Twenty isolates have shown maximum in-vitro activity against P. digitatum. Eight effective lead isolates were examined for their potential against postharvest natural infection of P. digitatum on kinnow fruit stored for fifteen days at the room temperature (25-30°C). All eight isolates showed least infection of P. digitatum with minimum quality loss as compared to a set of control and positive control (1% K sorbate). Isolates HAB-31 and HAB-53 which showed no postharvest decay of kinnow due to P. digitatum were further evaluated on kinnow by artificially spraying of P. digitatum on kinnow fruits. Yeast treated fruit showed least fruit decay with minimum quality changes as compared to control and positive control set.

Research paper thumbnail of Role of Endophytic and Rhizospheric Fluorescent Pseudomonas Associated with Mungbean in Suppressing the Root Rotting Fungi of Mungbeaan

Research paper thumbnail of Evaluation of biocontrol potential of fluorescent Pseudomonas associated with root nodules of mungbean

Crop Protection, 2015

Thirty two isolates of fluorescent Pseudomonas and sixteen rhizobia were isolated from the root n... more Thirty two isolates of fluorescent Pseudomonas and sixteen rhizobia were isolated from the root nodules of mungbean (Vigna radiata) collected from the experimental fields of Karachi University. Pseudomonas and rhizobia were characterized for their plant growth promoting and biocontrol potential through the determination of in vitro activity against root rotting fungi viz., Macrophomina phaseolina, Fusarium solani, Fusarium oxysporum and Rhizoctonia solani and nematicidal activity against the second stage juveniles of Meloidogyne javanica. Pseudomonas isolates NAFP-19, NAFP-21, NAFP-22, NAFP-27, NAFP-31 and NAFP-32, and rhizobial isolates NFB-102, NFB-103, NFB-107 and NFB-109 inhibited the growth of all the test fungi and showed maximum nematicidal activity against second stage juveniles of M. javanica. Strains of Pseudomonas (NAFP-19, NAFP-31 and NAFP-32) and rhizobia (NFB-103, NFB-107 and NFB-109) used as a soil drench significantly reduced root rot disease under screen house condition and thereby enhanced plant growth and yield in mungbean. In field plot experiments, mixed or separate application of NAFP and rhizobia showed effective control of M. phaseolina and F. oxysporum with some protection from F. solani and R. solani.

Research paper thumbnail of Effect of Endophytic Pseudomonas Aeruginosa and Trichoderma Harzianum on Soil-Borne Diseases , Mycorrhizae and Induction of Systemic Resistance in Okra Grown in Soil Amended with Vernonia Anthelmintica ( L . ) Seed ’ S Powder

Biostimulants are used in agricultural practices for plant growth improvement. These fertilizers ... more Biostimulants are used in agricultural practices for plant growth improvement. These fertilizers improve microbial activity and cause a negative impact on soil-borne pathogens. In recent years, stimulating plant’s natural defense is considered as most promising alternative strategy for crop productivity. The present study was carried out to examine the effect of endophytic Pseudomonas aeruginosa and Trichoderma harzianum in soil amendment with Vernonia anthelmintica seed’s powder, on root rotting fungi, plant growth, mycorrhizal population around roots, phosphorous uptake and stimulation of plant defense markers like poylphenol and antioxidant status in okra. Combine application of Vernonia with Pseudomonas aeruginosa and Trichoderma harzianum significantly (p<0.05) suppressed Rhizoctonia solani and Fusarium oxysporum with complete reduction of Macrophomina phaseolina and Fusarium solani. Pseudomonas aeruginosa and T. harzianum alone or in Vernonia amended soil significantly redu...

Research paper thumbnail of Effects of fungicides and storage temperature in maintaining the shelf life and fruit quality of stored mango (Mangifera indica L.)

Pakistan Journal of Botany, 2021

Research paper thumbnail of Impact of Endo-Nodule Fluorescent Pseudomonas and Rhizobia on Root Rotting Fungi and Growth of Soybean (Glycine Max L. Merr)

Rhizobia and fluorescent Pseudomonas associated with root nodules of mungbean (Vigna radiata) wer... more Rhizobia and fluorescent Pseudomonas associated with root nodules of mungbean (Vigna radiata) were studied for their synergistic effect on disease suppression and promotion of plant growth. Fluorescent Pseudomonas viz., NAFP-19, NAFP-31 and NAFP-32 and Rhizobium vignae (NFB-103), R. vignae (NFB-107) and R. vignae (NFB109) were applied simultaneously in naturally infested soil in screen house experiment caused suppression of root rotting fungi of soybean as compared to untreated control. Combined application of both bacteria produced healthier plants than untreated plants or plants inoculated with either component alone.

Research paper thumbnail of Role of Mungbean Root Nodule Associated Fluorescent Pseudomonas and Rhizobia in Suppressing the Root Rotting Fungi and Root Knot Nematodes in Chickpea ( C Icer Arietinum L . )

Three isolates each of fluorescent Pseudomonas (NAFP-19, NAFP-31 and NAFP-32) and rhizobia (NFB10... more Three isolates each of fluorescent Pseudomonas (NAFP-19, NAFP-31 and NAFP-32) and rhizobia (NFB103, NFB107 and NFB-109) which were originally isolated from root nodules of mungbean (Vigna radiata) showed significant biocontrol activity in the screen house and under field condition, against root rotting fungi viz., Macrophomina phaseolina, Fusarium solani, F. oxysporum and Rhizoctonia solani evaluated on chickpea. Biocontrol potential of these isolates was also evaluated against Meloidogyne incognita, the root knot nematode. Application of Pseudomonas and rhizobial isolates as a soil drench, separately or mixed significantly reduced root rot disease under screen house and field conditions. Nematode’s penetration in roots was also found significantly less in rhizobia or Pseudomonas treatments used separately or mixed as compared to control. Fluorescent Pseudomonas treated plants produced greater number of nodules per plant than control plants and about equal to rhizobia treated plants...

Research paper thumbnail of Protective role of epiphytic fluorescent Pseudomonas on natural postharvest decay of tomato at room temperature

Journal of applied botany and food quality, 2017

Following harvest, tomato fruits are susceptible to attack by many fungal pathogens, but healthy... more Following harvest, tomato fruits are susceptible to attack by many fungal pathogens, but healthy fruits may also harbor beneficial microflora, which can delayed the spoilage of fruit. In this study, 30 isolates of epiphytic fluorescent Pseudomonas were isolated from healthy fruits of lemon, melon, grapefruit, tomato and orange. Twelve isolates were identified on molecular basis by amplifying 16S rDNA using a genus-specific primer set PA-GS-F 5’-GACGGGTGAGTAATGCCTA-3’ and PA-GS-R F 5’-CACTGGTGTTCCTTCCTATA-3’ on a conserved sequence of the genus Pseudomonas with a product size ~618 bp. Three potential isolates were examined for their ability to delayed the postharvest natural spoilage and maintained the physiochemical properties during storage for fifteen days in season 2013 and 2014. All three isolates showed promising control of postharvest diseases of tomato in comparison with control in both seasons up to fifteen days of storage at room temperature (23±4 oC, Rh 25-70%). The...

Research paper thumbnail of Role of Rhizobia in Suppressing the Root Diseases of Soybean Under Soil Amendment

Planta Daninha, 2019

ABSTRACT: Rhizobia are soil bacteria, characterized by their unique ability to colonize the roots... more ABSTRACT: Rhizobia are soil bacteria, characterized by their unique ability to colonize the roots of leguminous crops, where they form nitrogen fixing nodules. Considerable evidence has been accumulated to identify the benefits associated with use of rhizobia as biocontrol agents against soil-borne pathogens, in addition to biological nitrogen fixation. In this study, out of four rhizobial isolates tested, Bradyrhizobium sp. inhibited the radial growth of all the test fungi viz: Macrophomina phaseolina, Rhizoctonia solani, Fusarium solani and F. oxysporum, while other 3 rhizobial isolates inhibited the growth of at least three fungi. Cell free culture filtrates of rhizobia also showed nematicidal activity by killing second-stage juveniles of Meloidogyne javanica at varying degrees. Application of rhizobial isolates alone or in mustard cake amended soil significantly (p<0.05) by suppression of root rotting fungi and root knot nematode on soybean roots. Rhizobia-treated plants show...

Research paper thumbnail of Role of fluorescent pseudomonas associated with root nodules of mungbean in the induction of nodulation by the rhizobia in mungbean

Pakistan Journal of Botany, 2019

Fluorescent Pseudomonas has been reported to associate with root nodules of a number of plants an... more Fluorescent Pseudomonas has been reported to associate with root nodules of a number of plants and play role in plant disease suppression and increased plant growth. However their role in nodule formation is still unclear. In a gnotobiotic experiment, combined effect of two isolates each of fluorescent Pseudomonas (NAFP-19 and NAFP-32) and rhizobia (Rhizobium vignae (NFB-103) and R. vignae (NFB-109) on nodules of mungbean was evaluated. The results showed an increase in the number of nodule primordia, mature nodules and the concentration of nitrogen and phosphorus in mungbean plants. In screen house experiment, garden soil which was naturally infested with root rotting fungi and rhizobia was used. Dual application of Pseudomonas and rhizobia in the garden soil produced greater number of nodules per plant and higher nitrogen fixation than when rhizobia were used alone. Dual application of bacteria also suppressed root rotting fungi. The results showed that nodule associated fluorescent Pseudomonas played a role in plant-rhizobia symbiosis.

Research paper thumbnail of Evaluation of biocontrol potential of epiphytic yeast against postharvest Penicillium digitatum rot of stored Kinnow fruit (Citrus reticulata) and their effect on its physiochemical properties

Postharvest Biology and Technology, 2019

Twenty five isolates of epiphytic yeasts were isolated from healthy surface of tomato, mango, lem... more Twenty five isolates of epiphytic yeasts were isolated from healthy surface of tomato, mango, lemon, orange, grape fruit and green chili and evaluated for their potential against postharvest Penicillium digitatum decay of kinnow fruits. Identification of yeast was confirmed by molecular biology tools. Twenty isolates have shown maximum in-vitro activity against P. digitatum. Eight effective lead isolates were examined for their potential against postharvest natural infection of P. digitatum on kinnow fruit stored for fifteen days at the room temperature (25-30°C). All eight isolates showed least infection of P. digitatum with minimum quality loss as compared to a set of control and positive control (1% K sorbate). Isolates HAB-31 and HAB-53 which showed no postharvest decay of kinnow due to P. digitatum were further evaluated on kinnow by artificially spraying of P. digitatum on kinnow fruits. Yeast treated fruit showed least fruit decay with minimum quality changes as compared to control and positive control set.

Research paper thumbnail of Role of Endophytic and Rhizospheric Fluorescent Pseudomonas Associated with Mungbean in Suppressing the Root Rotting Fungi of Mungbeaan

Research paper thumbnail of Evaluation of biocontrol potential of fluorescent Pseudomonas associated with root nodules of mungbean

Crop Protection, 2015

Thirty two isolates of fluorescent Pseudomonas and sixteen rhizobia were isolated from the root n... more Thirty two isolates of fluorescent Pseudomonas and sixteen rhizobia were isolated from the root nodules of mungbean (Vigna radiata) collected from the experimental fields of Karachi University. Pseudomonas and rhizobia were characterized for their plant growth promoting and biocontrol potential through the determination of in vitro activity against root rotting fungi viz., Macrophomina phaseolina, Fusarium solani, Fusarium oxysporum and Rhizoctonia solani and nematicidal activity against the second stage juveniles of Meloidogyne javanica. Pseudomonas isolates NAFP-19, NAFP-21, NAFP-22, NAFP-27, NAFP-31 and NAFP-32, and rhizobial isolates NFB-102, NFB-103, NFB-107 and NFB-109 inhibited the growth of all the test fungi and showed maximum nematicidal activity against second stage juveniles of M. javanica. Strains of Pseudomonas (NAFP-19, NAFP-31 and NAFP-32) and rhizobia (NFB-103, NFB-107 and NFB-109) used as a soil drench significantly reduced root rot disease under screen house condition and thereby enhanced plant growth and yield in mungbean. In field plot experiments, mixed or separate application of NAFP and rhizobia showed effective control of M. phaseolina and F. oxysporum with some protection from F. solani and R. solani.